FastQCFastQC Report
Thu 26 May 2016
SRR938022_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938022_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1597415
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199101.2463886967381674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159120.9961093391510659No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111880.7003815539480974No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49290.30856101889615406No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39670.2483387222481321No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33750.21127884738781094No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25530.1598207103351352No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23520.1472378812018167No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20470.12814453351195526No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20200.12645430273285277No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17190.10761135960285838No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89150.041.7782671
GTATCAA139150.034.311411
TATCAAC172100.027.7145962
ATCAACG172250.027.6327083
TCAACGC175600.027.3185354
CAACGCA177500.026.9993535
AACGCAG181300.026.5120476
GTACATG113100.025.2445471
GAGTACT116850.024.4087112-13
TACATGG114700.024.395382
GTATAGG4300.024.3058011
ACGCAGA203800.023.4918357
ACATGGG114850.023.4099433
CGCAGAG204400.023.3764028
ATGGGCG1852.5448026E-723.106355
GTACTTT123550.022.93127814-15
GCACCGT4000.022.5607836
GCAGAGT213300.022.2674089
GGACCGA2806.548362E-1122.0518936
AGTACTT118900.021.65100112-13
Produced by FastQC (version 0.11.5)