##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938022_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1597415 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.51517295129944 33.0 31.0 34.0 31.0 34.0 2 32.72950798634043 34.0 31.0 34.0 31.0 34.0 3 32.67301296156603 34.0 31.0 34.0 31.0 34.0 4 36.20641473881239 37.0 35.0 37.0 35.0 37.0 5 36.18767696559755 37.0 35.0 37.0 35.0 37.0 6 36.26193631586031 37.0 37.0 37.0 35.0 37.0 7 36.21799532369484 37.0 37.0 37.0 35.0 37.0 8 36.22500352131412 37.0 37.0 37.0 35.0 37.0 9 37.91354344362611 39.0 38.0 39.0 35.0 39.0 10-11 37.89192413993859 39.0 38.0 39.0 35.0 39.0 12-13 37.831908740058154 39.0 38.0 39.0 35.0 39.0 14-15 39.06210158286983 40.0 38.0 41.0 36.0 41.0 16-17 39.01107821073422 40.0 38.0 41.0 36.0 41.0 18-19 39.056213632650255 40.0 38.5 41.0 36.0 41.0 20-21 39.09277113336234 40.0 39.0 41.0 35.5 41.0 22-23 39.09167999549272 40.0 39.0 41.0 35.0 41.0 24-25 39.02417343019816 40.0 38.5 41.0 35.0 41.0 26-27 38.84188517072896 40.0 38.0 41.0 35.0 41.0 28-29 38.74362798646563 40.0 38.0 41.0 35.0 41.0 30-31 38.47690831750046 40.0 38.0 41.0 34.5 41.0 32-33 38.210405561485274 40.0 37.0 41.0 34.0 41.0 34-35 37.973062416466604 40.0 37.0 41.0 34.0 41.0 36-37 37.85524957509476 40.0 37.0 41.0 33.0 41.0 38-39 37.78102559447608 40.0 37.0 41.0 33.0 41.0 40-41 37.63284368808356 40.0 36.5 41.0 33.0 41.0 42-43 37.405236585358224 40.0 36.0 41.0 33.0 41.0 44-45 37.07980988033792 40.0 36.0 41.0 32.5 41.0 46-47 36.60060535302348 40.0 35.0 41.0 31.0 41.0 48-49 36.144627726670905 39.0 35.0 41.0 30.0 41.0 50-51 35.82196110591174 39.0 35.0 41.0 29.0 41.0 52-53 35.37649295893678 39.0 35.0 40.0 27.5 41.0 54-55 35.07733024918384 38.0 35.0 40.0 27.0 41.0 56-57 34.81537483997583 38.0 35.0 40.0 26.0 41.0 58-59 34.45213955046121 38.0 34.0 40.0 26.0 41.0 60-61 34.055465549027645 37.0 34.0 40.0 24.5 41.0 62-63 33.66193568984892 36.5 33.0 40.0 23.0 41.0 64-65 33.251106318646066 36.0 33.0 39.0 22.0 41.0 66-67 32.756079353205024 35.5 32.5 39.0 21.0 40.0 68-69 32.25495159366852 35.0 32.0 38.5 19.5 40.0 70-71 31.699086336362186 35.0 31.0 37.5 17.5 40.0 72-73 31.265079206092345 35.0 31.0 37.0 14.0 39.0 74-75 30.740212155263347 34.5 30.5 36.5 9.5 39.0 76-77 29.498958004025255 33.5 28.5 35.0 8.0 37.0 78-79 30.221844354785702 34.0 30.0 35.0 7.0 37.5 80-81 30.0506624765637 34.0 30.0 35.0 4.5 37.0 82-83 29.635581861945706 34.0 30.0 35.0 2.0 37.0 84-85 29.1798465020048 34.0 29.0 35.0 2.0 36.0 86-87 28.679727872844566 34.0 29.0 35.0 2.0 36.0 88-89 28.110605885133168 33.5 27.5 35.0 2.0 35.0 90-91 27.621326643358174 33.0 27.0 35.0 2.0 35.0 92-93 27.039456559503947 33.0 25.5 35.0 2.0 35.0 94-95 26.34140846304811 32.5 24.5 35.0 2.0 35.0 96-97 25.59161864637555 32.0 22.5 34.0 2.0 35.0 98-99 24.655196364125793 32.0 10.5 34.0 2.0 35.0 100-101 21.225508086502252 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.0037068143135670084 1101 2 0.030751681934809483 1101 3 0.19380842828995526 1101 4 0.007971665915469828 1101 5 -0.07657480656015281 1101 6 -0.08592194816256438 1101 7 0.0636305096692169 1101 8 0.07279906160324856 1101 9 0.1194236772820858 1101 10-11 0.048778793051681646 1101 12-13 0.05214555898867701 1101 14-15 0.1690034786478094 1101 16-17 0.18535023936298956 1101 18-19 0.15879725142118417 1101 20-21 0.17855430391134774 1101 22-23 0.14066165104264172 1101 24-25 0.048119219113175404 1101 26-27 0.11398290858687687 1101 28-29 0.0927802255230219 1101 30-31 -0.08031398043959825 1101 32-33 0.05583845916848418 1101 34-35 0.049225450456447106 1101 36-37 0.06645867327284805 1101 38-39 0.00692643518929259 1101 40-41 0.10783103844956088 1101 42-43 0.14311785987737125 1101 44-45 0.12373682140636078 1101 46-47 0.087768840235114 1101 48-49 0.010169084124107997 1101 50-51 0.10507170868621074 1101 52-53 -0.09906708133622999 1101 54-55 -0.10971332745191376 1101 56-57 -0.07152945893395213 1101 58-59 -0.098231844279006 1101 60-61 -0.06266041198946937 1101 62-63 -0.012273326418288377 1101 64-65 0.011725579064545855 1101 66-67 -0.1477661568974611 1101 68-69 0.0022671003237277887 1101 70-71 -0.030181120073120127 1101 72-73 0.011005380966182088 1101 74-75 0.07496162445004373 1101 76-77 0.16149120932046301 1101 78-79 0.1995659960914864 1101 80-81 0.36843028894535834 1101 82-83 0.46488541358080226 1101 84-85 0.5843193800418476 1101 86-87 0.6244541369754977 1101 88-89 0.773969727947815 1101 90-91 1.0041371873526082 1101 92-93 1.3068997674344018 1101 94-95 1.2418103016985071 1101 96-97 1.357709970790257 1101 98-99 1.682450445468838 1101 100-101 2.195228581771577 1102 1 0.03307610179525966 1102 2 0.08260914460881708 1102 3 0.15771962019690733 1102 4 0.033435821178137815 1102 5 0.029893910300927473 1102 6 -0.021473800575499524 1102 7 0.05530180526415762 1102 8 0.0035906925009214774 1102 9 0.07410057808836257 1102 10-11 0.093398295147324 1102 12-13 0.04837172000805623 1102 14-15 0.20788310968014656 1102 16-17 0.10013924439589772 1102 18-19 0.20724950613006143 1102 20-21 0.16891603361560925 1102 22-23 0.15986917852164595 1102 24-25 0.17390992422519247 1102 26-27 0.19047805242030336 1102 28-29 0.003428443806448911 1102 30-31 -0.010743713308357883 1102 32-33 0.07901171574576438 1102 34-35 0.08155556878868708 1102 36-37 0.1408851069781818 1102 38-39 0.2205977644491739 1102 40-41 0.21151229886464762 1102 42-43 0.23621486538159076 1102 44-45 0.3866515312988028 1102 46-47 0.34118330605677727 1102 48-49 0.20769134506100073 1102 50-51 0.26919667291721794 1102 52-53 0.16013270320820538 1102 54-55 0.25323504737345104 1102 56-57 0.32838469548709526 1102 58-59 0.16159074152449904 1102 60-61 0.07214071241929076 1102 62-63 0.01011722046854402 1102 64-65 0.14395133610472755 1102 66-67 0.20899123676343123 1102 68-69 0.2782589534099671 1102 70-71 0.47977528383874457 1102 72-73 0.3547125633796213 1102 74-75 0.34053754729001895 1102 76-77 0.18860494629634061 1102 78-79 0.255921093803952 1102 80-81 0.4409597036804982 1102 82-83 0.43908629223555096 1102 84-85 0.5045621042082189 1102 86-87 0.6532932017636632 1102 88-89 0.7516683931332295 1102 90-91 0.7313502358806154 1102 92-93 0.9934018125790764 1102 94-95 1.139990712074038 1102 96-97 1.5482602241188772 1102 98-99 1.7048830735218239 1102 100-101 2.207659358253256 1103 1 0.20403669081605358 1103 2 0.266130936826265 1103 3 0.3723953198224379 1103 4 0.14731562402115372 1103 5 0.09931946802080205 1103 6 0.13644780216215935 1103 7 0.155962942714595 1103 8 0.13627554759380445 1103 9 0.1812301634788156 1103 10-11 0.2539640393251048 1103 12-13 0.18310859220311926 1103 14-15 0.2479903454395611 1103 16-17 0.13418639382766173 1103 18-19 0.3750003728775866 1103 20-21 0.28623424123481556 1103 22-23 0.30214032246502853 1103 24-25 0.4167809513771559 1103 26-27 0.4054906503760449 1103 28-29 0.3531125760992708 1103 30-31 0.4416372390725982 1103 32-33 0.3543127803444861 1103 34-35 0.3457308212582575 1103 36-37 0.28776869533334093 1103 38-39 0.3416892333667292 1103 40-41 0.34644926824977773 1103 42-43 0.45828599380723745 1103 44-45 0.6344371904556994 1103 46-47 0.4790992734041879 1103 48-49 0.5382539448543682 1103 50-51 0.4409066054967141 1103 52-53 0.41069726313730825 1103 54-55 0.5332118524338654 1103 56-57 0.4956210266030254 1103 58-59 0.592645008699165 1103 60-61 0.675815558569056 1103 62-63 0.6255202121257213 1103 64-65 0.8285369103224127 1103 66-67 0.7221016863287275 1103 68-69 0.7115490004123828 1103 70-71 0.7507293390737324 1103 72-73 0.6711315587283835 1103 74-75 0.6187053104076163 1103 76-77 0.5087604503983343 1103 78-79 0.7985907986810155 1103 80-81 1.0783100901947655 1103 82-83 1.204862322225626 1103 84-85 1.2687904436873936 1103 86-87 1.2591282808428872 1103 88-89 1.4009041443756658 1103 90-91 1.4782109559854781 1103 92-93 1.7448994787924619 1103 94-95 1.6768555566311925 1103 96-97 1.4287736163379563 1103 98-99 1.5420550060114842 1103 100-101 2.0919153846497522 1104 1 0.1011920438242484 1104 2 0.16321645999874335 1104 3 0.15426896457233852 1104 4 0.10872912690622627 1104 5 0.030515028313352843 1104 6 0.0589954885894457 1104 7 0.08918724349742035 1104 8 0.13147198994743547 1104 9 0.10922825234646893 1104 10-11 0.10478545870839184 1104 12-13 0.04944142325167178 1104 14-15 0.12192727807214965 1104 16-17 0.19240977579686813 1104 18-19 0.19472362621288397 1104 20-21 0.2319250053202282 1104 22-23 0.17988298114414647 1104 24-25 0.21341993112650925 1104 26-27 0.1948948916197466 1104 28-29 0.2035664700314257 1104 30-31 0.23014655584278643 1104 32-33 0.2815997074641956 1104 34-35 0.258746735110293 1104 36-37 0.3649361766814252 1104 38-39 0.42470404464240374 1104 40-41 0.5459843485540858 1104 42-43 0.5656144513029204 1104 44-45 0.5661546368888679 1104 46-47 0.5189610838345615 1104 48-49 0.6531019730803322 1104 50-51 0.7420055065956177 1104 52-53 0.692085774291705 1104 54-55 0.7660024731843507 1104 56-57 0.8139954615326417 1104 58-59 0.6962008174389211 1104 60-61 0.6557155916463486 1104 62-63 0.5703656676735136 1104 64-65 0.7088926749591096 1104 66-67 0.7359408571913093 1104 68-69 0.9068648885376334 1104 70-71 0.9065178649291532 1104 72-73 0.8784875806328998 1104 74-75 0.8334982098158967 1104 76-77 0.8494400049574864 1104 78-79 0.9257968702014665 1104 80-81 1.1087650867032757 1104 82-83 1.1303906400616377 1104 84-85 1.3250935051744008 1104 86-87 1.4527756034199761 1104 88-89 1.562986543581811 1104 90-91 1.6812467162118772 1104 92-93 1.82569994922504 1104 94-95 1.8888865022741754 1104 96-97 2.21351212197947 1104 98-99 2.618513163238873 1104 100-101 2.852931960047595 1105 1 0.11197504736264818 1105 2 0.17934730481035643 1105 3 0.20695769181192958 1105 4 0.11338599337344846 1105 5 0.08797755621810666 1105 6 0.049445609320208916 1105 7 0.04687680598128452 1105 8 0.10978525210783374 1105 9 0.05667012693833584 1105 10-11 0.09821929976899924 1105 12-13 0.0949273076234931 1105 14-15 0.1298248923107792 1105 16-17 0.1372749218127538 1105 18-19 0.1580188167245069 1105 20-21 0.1608658396533258 1105 22-23 0.20718597739313793 1105 24-25 0.2491285219934909 1105 26-27 0.16679613537440474 1105 28-29 0.14053969665597776 1105 30-31 0.23494845108796625 1105 32-33 0.28792402522148564 1105 34-35 0.2720954571865519 1105 36-37 0.3070552673282023 1105 38-39 0.42713954100529605 1105 40-41 0.5420811002131813 1105 42-43 0.4366216040311315 1105 44-45 0.3738741922351849 1105 46-47 0.3359879179550518 1105 48-49 0.5137999335656858 1105 50-51 0.6060205258062368 1105 52-53 0.6677718135633768 1105 54-55 0.5979513258624465 1105 56-57 0.644375953263733 1105 58-59 0.7787795202286318 1105 60-61 0.6792926391337772 1105 62-63 0.8424343399388476 1105 64-65 0.8248420439700226 1105 66-67 0.8812346353808067 1105 68-69 0.9456111109555891 1105 70-71 1.009475388961409 1105 72-73 1.0809002976065827 1105 74-75 1.0035209132738068 1105 76-77 1.030415798059888 1105 78-79 1.3031446659159052 1105 80-81 1.2935795865438173 1105 82-83 1.282407754976063 1105 84-85 1.357602351170545 1105 86-87 1.432428673322331 1105 88-89 1.5505702578371547 1105 90-91 1.5786688486664282 1105 92-93 1.847243556569115 1105 94-95 1.6833989474138136 1105 96-97 1.809140757190331 1105 98-99 1.972660910357277 1105 100-101 2.1117039231753587 1106 1 0.06517854717949234 1106 2 0.044423290027260975 1106 3 0.13975477569496775 1106 4 0.01170383582808654 1106 5 0.008362827921466476 1106 6 0.04278741027258803 1106 7 0.06658263315251389 1106 8 0.05006694686257873 1106 9 0.05855077222451399 1106 10-11 0.07567575307748342 1106 12-13 0.05314883959743355 1106 14-15 0.03674635191785569 1106 16-17 0.16499433787462436 1106 18-19 0.04936688053839333 1106 20-21 0.03108903040704547 1106 22-23 0.0037929984688673812 1106 24-25 0.031568908664887374 1106 26-27 0.008348791953388002 1106 28-29 -0.024452307251237926 1106 30-31 0.04108042682272384 1106 32-33 0.04366498159587451 1106 34-35 -0.1746873439177108 1106 36-37 -0.11991300739192923 1106 38-39 0.07762503933907539 1106 40-41 0.10345781297030499 1106 42-43 -0.016322059995985683 1106 44-45 0.20455841655462592 1106 46-47 0.0558998269216886 1106 48-49 0.2612313562745854 1106 50-51 0.30951029170088873 1106 52-53 0.3839745480186423 1106 54-55 0.40350332429003544 1106 56-57 0.5356547771680198 1106 58-59 0.5323565432996773 1106 60-61 0.5497309947678204 1106 62-63 0.546926595492188 1106 64-65 0.7214519686548755 1106 66-67 0.6628690843949201 1106 68-69 0.3594893201881746 1106 70-71 0.35566600270992055 1106 72-73 0.5782606191180086 1106 74-75 0.5621131393443477 1106 76-77 0.6516850326089667 1106 78-79 0.6692596220341827 1106 80-81 0.8247636038880408 1106 82-83 0.8179058653965967 1106 84-85 0.8540193719782536 1106 86-87 0.8253230951169677 1106 88-89 1.0492599111611156 1106 90-91 1.0290833947057614 1106 92-93 0.8938356873417135 1106 94-95 1.0760249714944123 1106 96-97 1.3702475832030956 1106 98-99 1.6889325124645538 1106 100-101 1.9553218647994157 1107 1 0.1276011569914317 1107 2 0.030482767568130953 1107 3 0.12666658319041346 1107 4 0.04659413038552174 1107 5 0.08342345850844879 1107 6 -0.00949243697948532 1107 7 0.07001505721356693 1107 8 0.07155858565373308 1107 9 0.02451136360648576 1107 10-11 0.07561095289668884 1107 12-13 0.08834634838246558 1107 14-15 0.0913855394571641 1107 16-17 0.01909465505254815 1107 18-19 0.13137087757238675 1107 20-21 0.10363707459679716 1107 22-23 0.10744928098177553 1107 24-25 0.07738606601994036 1107 26-27 0.06042413055688911 1107 28-29 0.154740408847438 1107 30-31 0.10227742875842694 1107 32-33 0.03177496067931429 1107 34-35 0.31107141746383604 1107 36-37 0.3401339808617294 1107 38-39 0.2511324872083236 1107 40-41 0.33528488204549234 1107 42-43 0.24961228641025457 1107 44-45 0.20870508661954545 1107 46-47 0.27126385953621934 1107 48-49 0.1834207792966609 1107 50-51 0.2237482451848294 1107 52-53 0.13919538486875638 1107 54-55 0.2503456602156149 1107 56-57 0.27557248274344204 1107 58-59 0.3200803566260575 1107 60-61 0.2997479619460748 1107 62-63 0.2968495211824447 1107 64-65 0.343801569059778 1107 66-67 0.3320479162877277 1107 68-69 0.3166829344642892 1107 70-71 0.5410595004540149 1107 72-73 0.44436088286737885 1107 74-75 0.5807418375574507 1107 76-77 0.6017286057667448 1107 78-79 0.5278728476247316 1107 80-81 0.5997356802446774 1107 82-83 0.887000005799635 1107 84-85 0.9273962808760601 1107 86-87 0.9603643253601355 1107 88-89 1.0883364056987688 1107 90-91 1.0427683264796528 1107 92-93 1.3844613213546246 1107 94-95 1.4275491656075978 1107 96-97 1.5331288844340456 1107 98-99 1.6866518710041376 1107 100-101 1.869336176433773 1108 1 0.02443502732635494 1108 2 0.051130464519197005 1108 3 0.13352469078209594 1108 4 0.05685598447621487 1108 5 0.04248712249548703 1108 6 0.026458871102036596 1108 7 0.058998060561258114 1108 8 0.00870952754376475 1108 9 0.008302804305039047 1108 10-11 0.06710192634440659 1108 12-13 0.002527436528289684 1108 14-15 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-3.0723086287880648 2316 100-101 -3.692128205561694 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 6.0 4 10.0 5 30.0 6 82.0 7 204.0 8 389.0 9 804.0 10 1366.0 11 2267.0 12 3444.0 13 5399.0 14 7217.0 15 9657.0 16 13384.0 17 20109.0 18 20028.0 19 12734.0 20 10082.0 21 10218.0 22 10489.0 23 11245.0 24 12540.0 25 14657.0 26 17079.0 27 20274.0 28 24709.0 29 30306.0 30 36715.0 31 45728.0 32 57446.0 33 76572.0 34 109446.0 35 176911.0 36 247772.0 37 344375.0 38 229057.0 39 14659.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.94955913147179 19.046396834886366 14.759345567682788 21.244698465959065 2 15.938645359458956 20.973625192993264 39.44832413602253 23.639405311525252 3 19.45766794964578 26.102844507438693 30.075175731410788 24.36431181150474 4 12.512841058835681 17.35841969682268 36.35561203569517 33.77312720864647 5 12.94804642774196 38.4775451716438 33.08718441824782 15.487223982366421 6 30.85349195637449 36.436891125281235 16.97894221535557 15.730674702988706 7 27.894754963487884 34.99572747219727 19.662830260139035 17.44668730417581 8 27.3720697152208 33.771166254437645 19.12592422873734 19.73083980160422 9 27.71771893966189 16.38440856007988 20.066294607224798 35.83157789303343 10-11 25.424169674129764 27.247646979651503 25.577542467048325 21.750640879170412 12-13 26.719575063461903 25.170760259544327 27.007853313008827 21.101811363984936 14-15 23.207901515886604 25.743435487960237 26.794383425722184 24.254279570430977 16-17 21.212865786285967 29.55493719540633 27.0894538989555 22.142743119352204 18-19 21.135928985266823 26.594654488658236 31.32551653765615 20.943899988418792 20-21 22.23933667832091 25.438724439172038 31.58484175996845 20.7370971225386 22-23 21.795306792536692 25.765533690368503 31.562743557560186 20.876415959534622 24-25 21.403841332274524 26.08811194020741 31.79276675649036 20.715279971027712 26-27 21.07722882377913 26.398630516098574 31.741226900290403 20.782913759831896 28-29 20.710186226220912 26.62431482923629 31.975345120732783 20.69015382381001 30-31 20.81323313805939 26.277095355368115 31.841119459326134 21.06855204724636 32-33 20.64638649969919 26.410595752859255 31.71614110058052 21.226876646861033 34-35 21.36605911326965 26.439404421943546 31.36946513554278 20.825071329244018 36-37 21.415902665260955 26.151135797336995 31.347962676830537 21.084998860571517 38-39 20.98905230717406 26.3707626568121 31.459597412676583 21.180587623337253 40-41 20.993910743077187 26.45401750074355 31.46774572264922 21.084326033530047 42-43 21.13043704209007 26.395679261827983 31.005028067454553 21.468855628627395 44-45 20.954945439872734 26.632366571932376 30.935571813110396 21.477116175084497 46-47 21.336834471077896 26.974327737418424 30.35688629951881 21.33195149198487 48-49 21.302939569090235 27.013398555537684 30.162775891739823 21.520885983632265 50-51 21.162096261772927 27.232998312899277 29.95333085015478 21.65157457517301 52-53 21.53300801607597 27.32258054419171 29.611747729926165 21.532663709806155 54-55 21.049476810972727 27.2836113345624 29.663205866978835 22.003705987486033 56-57 21.317159285470588 27.48947518334309 29.451081904201477 21.74228362698485 58-59 21.522772729691408 27.06882682333645 29.479221116616532 21.929179330355606 60-61 21.262132883439808 27.26958867920985 29.8781468810547 21.590131556295642 62-63 21.083719634534546 27.39310072836426 29.75022145153263 21.77295818556856 64-65 20.942209757639688 27.45607747517082 29.644801131828615 21.95691163536088 66-67 21.061497891120798 27.583075755323062 29.30589312403653 22.049533229519614 68-69 21.022966829169047 27.33523745432066 29.523588928971073 22.118206787539226 70-71 21.086286281273182 27.518929019697445 29.312639483164986 22.082145215864383 72-73 21.178497760444216 27.48518700525537 29.09704115711947 22.239274077180944 74-75 20.95742183465161 27.90211059743398 28.845572377873 22.294895190041412 76-77 20.92984603249625 27.535267917228772 29.23072589151848 22.304160158756492 78-79 21.029913954733114 27.622753010332318 29.05043460841422 22.296898426520347 80-81 20.995203180142887 27.936929252775972 28.990633289773303 22.077234277307834 82-83 21.092538557993336 27.75821820346701 28.89525409744215 22.253989141097495 84-85 20.84907556207191 27.66183219822343 28.80782471457396 22.681267525130703 86-87 21.006263598230753 27.49804448403645 28.870520898119416 22.62517101961338 88-89 20.871089693147606 27.62291279712886 28.618146966376262 22.887850543347273 90-91 20.889767984329037 27.524980451986387 28.86992761131099 22.715323952373588 92-93 21.157061112726584 27.268991088959844 28.819760604807627 22.75418719350595 94-95 20.793427615059617 27.122512615726325 28.98806255872784 23.095997210486217 96-97 20.7857158731058 26.999495120067834 28.961108594884404 23.25368041194196 98-99 20.698085840150092 27.206899163709274 28.577836261300316 23.517178734840314 100-101 20.723551385370087 26.77202102705007 28.93532560688782 23.569101980692025 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5364.0 1 3429.0 2 2107.0 3 2744.0 4 2118.0 5 1878.5 6 3924.5 7 8128.5 8 12076.0 9 12659.5 10 9047.5 11 5445.0 12 4632.5 13 4715.5 14 4667.0 15 4450.0 16 4312.0 17 4252.0 18 4348.5 19 4445.5 20 4633.5 21 5046.5 22 5765.0 23 6550.0 24 7263.5 25 8338.0 26 9863.0 27 11677.0 28 13340.5 29 15185.0 30 17650.5 31 20168.0 32 23214.0 33 25787.5 34 27834.5 35 29757.5 36 32601.5 37 36236.0 38 38294.5 39 38650.0 40 39199.0 41 41907.0 42 44775.5 43 47698.0 44 50535.0 45 53497.0 46 54235.0 47 55628.0 48 61623.5 49 61549.0 50 56609.5 51 53174.5 52 51012.0 53 51557.5 54 52109.0 55 49273.5 56 44754.0 57 40858.5 58 37607.0 59 33635.5 60 28794.0 61 24028.0 62 19784.5 63 15522.0 64 11810.5 65 9310.0 66 6940.5 67 5536.5 68 4026.5 69 2465.5 70 1744.5 71 1402.5 72 1272.0 73 1096.0 74 876.0 75 694.0 76 452.0 77 234.5 78 116.5 79 67.5 80 38.0 81 17.0 82 11.5 83 9.0 84 3.0 85 2.5 86 2.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.013083638253052587 3 0.0056967037369750504 4 0.0 5 5.008091197340704E-4 6 6.26011399667588E-5 7 0.0 8 1.252022799335176E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0016589302091191082 26-27 0.01984456136946254 28-29 1.878034199002764E-4 30-31 6.26011399667588E-5 32-33 0.0036308661180720105 34-35 0.015274678151889145 36-37 0.007699940215911331 38-39 0.020063665359346193 40-41 0.02128438758869799 42-43 0.01755961976067584 44-45 0.00879546016532961 46-47 0.0018467336290193845 48-49 2.504045598670352E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.56502849916897E-4 68-69 1.56502849916897E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.56502849916897E-4 82-83 0.0010016182394681408 84-85 0.00413167523780608 86-87 6.573119696509673E-4 88-89 1.252022799335176E-4 90-91 0.00413167523780608 92-93 9.077165295180026E-4 94-95 2.504045598670352E-4 96-97 3.4430626981717333E-4 98-99 2.504045598670352E-4 100-101 0.0027544501585373866 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1597415.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.62267319060071 #Duplication Level Percentage of deduplicated Percentage of total 1 72.20084231328443 32.93995432945071 2 14.581102423143925 13.304577412195426 3 5.290409593766573 7.240878838224932 4 2.4891067648891236 4.542388178841995 5 1.4077096897044519 3.2111739560314074 6 0.8331689534903539 2.2806836926587133 7 0.5758024711223871 1.8388753573649879 8 0.4093557847111554 1.4940724147647155 9 0.2956729352005314 1.2140450724563554 >10 1.676320570666211 14.214228320331229 >50 0.1511301290447917 4.726745876538301 >100 0.08265107651891516 6.813445193525597 >500 0.0043933351557366605 1.3749262248704974 >1k 0.001922084130634789 1.8594795151806502 >5k 0.0 0.0 >10k+ 4.118751708503119E-4 2.94452561756467 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19910 1.2463886967381674 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15912 0.9961093391510659 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11188 0.7003815539480974 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4929 0.30856101889615406 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3967 0.2483387222481321 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3375 0.21127884738781094 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2553 0.1598207103351352 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2352 0.1472378812018167 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2047 0.12814453351195526 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2020 0.12645430273285277 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1719 0.10761135960285838 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.26011399667588E-5 0.0 14-15 0.0 0.0 0.0 6.26011399667588E-5 0.0 16-17 0.0 0.0 0.0 1.252022799335176E-4 0.0 18-19 0.0 0.0 0.0 2.504045598670352E-4 0.0 20-21 0.0 0.0 0.0 3.756068398005528E-4 0.0 22-23 0.0 0.0 0.0 0.0010642193794348997 0.0 24-25 0.0 0.0 0.0 0.002723149588554008 0.0 26-27 0.0 0.0 0.0 0.0049454900573739444 0.0 28-29 0.0 0.0 0.0 0.011456008613916858 0.0 30-31 0.0 0.0 0.0 0.027294097025506833 0.0 32-33 0.0 0.0 0.0 0.06087960861767293 0.0 34-35 0.0 0.0 0.0 0.10864427841230989 0.0 36-37 0.0 0.0 0.0 0.1845481606220049 0.0 38-39 0.0 0.0 0.0 0.2976371199719547 0.0 40-41 0.0 0.0 0.0 0.4209300651364861 0.0 42-43 0.0 0.0 0.0 0.5397470287933943 0.0 44-45 0.0 0.0 0.0 0.6754036991013607 0.0 46-47 0.0 0.0 0.0 0.8336593809373269 0.0 48-49 0.0 0.0 0.0 0.9980186739200521 0.0 50-51 0.0 0.0 0.0 1.1795306792536693 0.0 52-53 0.0 0.0 0.0 1.348397254314001 0.0 54-55 0.0 0.0 0.0 1.5271235089190975 0.0 56-57 0.0 0.0 0.0 1.7307337166609804 0.0 58-59 0.0 0.0 0.0 1.9493055968549187 0.0 60-61 0.0 0.0 0.0 2.181023716441876 0.0 62-63 0.0 0.0 0.0 2.404353283273289 0.0 64-65 0.0 0.0 0.0 2.630437300263238 0.0 66-67 0.0 0.0 0.0 2.8803410510105385 0.0 68-69 0.0 0.0 0.0 3.160919360341552 0.0 70-71 0.0 0.0 0.0 3.4576487637839888 0.0 72-73 0.0 0.0 0.0 3.7525940347373723 0.0 74-75 0.0 0.0 0.0 4.044409248692419 0.0 76-77 0.0 0.0 0.0 4.3498402105902345 0.0 78-79 0.0 0.0 0.0 4.691423330818854 0.0 80-81 0.0 0.0 0.0 5.055167254595706 0.0 82-83 0.0 0.0 0.0 5.417659155573222 0.0 84-85 0.0 0.0 0.0 5.774485653383748 0.0 86-87 0.0 0.0 0.0 6.1433002694979075 0.0 88-89 0.0 0.0 0.0 6.531114331591978 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8915 0.0 41.778267 1 GTATCAA 13915 0.0 34.31141 1 TATCAAC 17210 0.0 27.714596 2 ATCAACG 17225 0.0 27.632708 3 TCAACGC 17560 0.0 27.318535 4 CAACGCA 17750 0.0 26.999353 5 AACGCAG 18130 0.0 26.512047 6 GTACATG 11310 0.0 25.244547 1 GAGTACT 11685 0.0 24.40871 12-13 TACATGG 11470 0.0 24.39538 2 GTATAGG 430 0.0 24.305801 1 ACGCAGA 20380 0.0 23.491835 7 ACATGGG 11485 0.0 23.409943 3 CGCAGAG 20440 0.0 23.376402 8 ATGGGCG 185 2.5448026E-7 23.10635 5 GTACTTT 12355 0.0 22.931278 14-15 GCACCGT 400 0.0 22.560783 6 GCAGAGT 21330 0.0 22.267408 9 GGACCGA 280 6.548362E-11 22.051893 6 AGTACTT 11890 0.0 21.651001 12-13 >>END_MODULE