FastQCFastQC Report
Thu 26 May 2016
SRR938021_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938021_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1601055
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207461.2957706012597943No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159940.9989663065915911No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112080.7000384121719742No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51650.32259978576626036No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38050.23765579570970394No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33620.209986540125105No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26150.16332980441021702No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25970.16220554571829202No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23550.1470905121935224No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20800.12991433773355693No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17670.11036472825730534No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92650.037.912341
GTATCAA143450.033.60231
GTACATG113950.027.349351
ATCAACG177650.027.1768173
ACCGTAT700.00835549427.1674828
TATCAAC178250.027.1386552
TCAACGC178750.027.0361614
CAACGCA180200.026.8449825
AACGCAG183450.026.3693966
TACATGG114900.026.3031182
ACATGGG116450.024.8104233
GAGTACT113000.024.48526612-13
ACGCAGA205200.023.4935177
CATGGGA75200.023.3173224
CGCAGAG206800.023.265778
ATGGGAG25100.023.0970345
GTACTTT118550.023.04060214-15
TATCTCG1050.00219141322.6282425
GCAGAGT215150.022.0534559
AGTACTT113100.022.02566112-13
Produced by FastQC (version 0.11.5)