##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938021_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1601055 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.096352092838785 33.0 31.0 34.0 31.0 34.0 2 32.2521312509564 34.0 31.0 34.0 31.0 34.0 3 32.30730861837976 34.0 31.0 34.0 30.0 34.0 4 35.82471932569462 37.0 35.0 37.0 35.0 37.0 5 35.8653631511722 37.0 35.0 37.0 35.0 37.0 6 35.915533819887514 37.0 37.0 37.0 35.0 37.0 7 35.870341118824776 37.0 36.0 37.0 35.0 37.0 8 35.864935932869265 37.0 36.0 37.0 35.0 37.0 9 37.540827142103176 39.0 38.0 39.0 35.0 39.0 10-11 37.484675729440895 39.0 38.0 39.0 35.0 39.0 12-13 37.38918057156063 39.0 37.0 39.0 35.0 39.0 14-15 38.63372089028796 40.0 38.0 41.0 34.5 41.0 16-17 38.58453956922155 40.0 38.0 41.0 34.5 41.0 18-19 38.590156490564034 40.0 38.0 41.0 34.0 41.0 20-21 38.52898651201863 40.0 38.0 41.0 34.0 41.0 22-23 38.38697421387772 40.0 38.0 41.0 34.0 41.0 24-25 38.321246303218814 40.0 38.0 41.0 34.0 41.0 26-27 38.30620746944983 40.0 38.0 41.0 34.0 41.0 28-29 38.2051063205199 40.0 38.0 41.0 34.0 41.0 30-31 37.96713136025933 40.0 37.5 41.0 34.0 41.0 32-33 37.687091011864055 40.0 37.0 41.0 33.0 41.0 34-35 37.45846207656827 40.0 36.0 41.0 33.0 41.0 36-37 37.24360874548344 40.0 36.0 41.0 32.5 41.0 38-39 37.01049339341871 40.0 35.0 41.0 31.5 41.0 40-41 36.775004606337696 39.0 35.0 41.0 31.0 41.0 42-43 36.46080802970542 39.0 35.0 41.0 30.5 41.0 44-45 36.14756925901983 39.0 35.0 41.0 30.0 41.0 46-47 35.86018718907221 39.0 35.0 41.0 28.5 41.0 48-49 35.51931226597462 39.0 35.0 41.0 27.0 41.0 50-51 34.83520616093763 38.5 34.5 40.5 25.5 40.5 52-53 34.894267529847504 38.5 35.0 40.0 26.0 41.0 54-55 35.02321063298887 39.0 35.0 41.0 26.0 41.0 56-57 34.83720203240988 38.5 35.0 41.0 25.5 41.0 58-59 34.5995424891712 38.0 35.0 40.0 25.0 41.0 60-61 34.25142234339232 38.0 34.0 40.0 23.0 41.0 62-63 33.873672047493685 37.0 34.0 40.0 22.5 41.0 64-65 33.48412640415226 36.5 34.0 40.0 21.5 41.0 66-67 33.08673062449448 36.0 33.0 39.0 20.0 41.0 68-69 32.63732788692456 35.5 33.0 39.0 18.5 41.0 70-71 32.185093578921396 35.0 32.5 38.5 14.5 40.0 72-73 31.73677169116614 35.0 32.0 37.5 10.0 40.0 74-75 31.25369209677369 35.0 31.5 37.0 7.0 39.0 76-77 30.754931279687455 35.0 31.0 36.5 3.5 39.0 78-79 30.22814300570561 35.0 30.5 36.0 2.0 38.5 80-81 29.717749234098765 34.0 30.0 35.0 2.0 37.0 82-83 29.227268894572642 34.0 29.0 35.0 2.0 37.0 84-85 28.765506806449494 34.0 29.0 35.0 2.0 36.0 86-87 28.30473187991668 34.0 29.0 35.0 2.0 36.0 88-89 27.795782156140795 34.0 27.0 35.0 2.0 35.5 90-91 27.243274590816682 33.0 26.0 35.0 2.0 35.0 92-93 26.67820749443336 33.0 25.0 35.0 2.0 35.0 94-95 26.143185274709488 33.0 24.5 35.0 2.0 35.0 96-97 25.38164647685432 32.5 21.0 35.0 2.0 35.0 98-99 24.423954829784112 32.0 8.5 34.5 2.0 35.0 100-101 21.646088985075465 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2825370022017779 1101 2 0.22808687594964283 1101 3 0.1436230228415738 1101 4 0.13393776030864757 1101 5 0.16579594381551743 1101 6 0.11436586417093508 1101 7 0.1255436189095036 1101 8 0.08097794193108143 1101 9 0.04958845078562035 1101 10-11 0.10277111144733908 1101 12-13 0.03400802805391834 1101 14-15 0.16148435350663704 1101 16-17 0.2284953708597115 1101 18-19 -0.003451220840226199 1101 20-21 0.3682865332511369 1101 22-23 0.3246498486347278 1101 24-25 0.19657830184920044 1101 26-27 0.3754459208791374 1101 28-29 0.5129091239925501 1101 30-31 0.3933880869420108 1101 32-33 0.47400011803268427 1101 34-35 0.5352458377858298 1101 36-37 0.5248135814907755 1101 38-39 0.6729392953120694 1101 40-41 0.5335625893436386 1101 42-43 0.5281390161871684 1101 44-45 0.6485657551210693 1101 46-47 0.6278187554129389 1101 48-49 0.518136679001266 1101 50-51 0.6750659895207676 1101 52-53 0.6172020860144869 1101 54-55 0.6022998211022639 1101 56-57 0.6845654664175385 1101 58-59 0.5447839177370355 1101 60-61 0.45810300164691853 1101 62-63 0.4406553069273613 1101 64-65 0.40450874641738466 1101 66-67 0.4198567535847104 1101 68-69 0.38136085628299554 1101 70-71 0.4816981129757565 1101 72-73 0.5223222519772293 1101 74-75 0.5986204231303027 1101 76-77 0.46839774315090565 1101 78-79 0.5774520328753958 1101 80-81 0.41249662766584194 1101 82-83 0.31629853088336546 1101 84-85 0.5682867602475703 1101 86-87 0.6680924282909935 1101 88-89 0.7264476599864231 1101 90-91 0.6195140937972106 1101 92-93 0.7806334595038393 1101 94-95 0.854821920211343 1101 96-97 0.7203743344838642 1101 98-99 0.682426448698731 1101 100-101 0.6050117171151967 1102 1 0.21038022753943864 1102 2 0.19532294118667437 1102 3 0.11477442315698738 1102 4 0.180304580843476 1102 5 0.12325758956168187 1102 6 0.13165803417268762 1102 7 0.055362269496328054 1102 8 0.13717149521045258 1102 9 0.25486085885587784 1102 10-11 0.22448725493347865 1102 12-13 0.08981883069281338 1102 14-15 0.1307426379582779 1102 16-17 0.22941445233495728 1102 18-19 0.2803844157160782 1102 20-21 0.2690744476416995 1102 22-23 0.25358997251635174 1102 24-25 0.22906627220054787 1102 26-27 0.29550982943749204 1102 28-29 0.3819776889104176 1102 30-31 0.41402638542704295 1102 32-33 0.32338138182490894 1102 34-35 0.3526296279722416 1102 36-37 0.4033894803428595 1102 38-39 0.5050576653270724 1102 40-41 0.5878697458927249 1102 42-43 0.5428697565108962 1102 44-45 0.3827383562224327 1102 46-47 0.40971088260422306 1102 48-49 0.4001069401163946 1102 50-51 0.43795640717251416 1102 52-53 0.49750888165012697 1102 54-55 0.46754015970820006 1102 56-57 0.43693180125499964 1102 58-59 0.3320535114502263 1102 60-61 0.38309371484454857 1102 62-63 0.5346701427741394 1102 64-65 0.448027134286086 1102 66-67 0.38774889421425485 1102 68-69 0.3974975752917089 1102 70-71 0.36613357617842013 1102 72-73 0.49154190141123877 1102 74-75 0.5870668730761146 1102 76-77 0.49551784574247293 1102 78-79 0.4630239895158681 1102 80-81 0.6055742883707183 1102 82-83 0.7890286876023573 1102 84-85 0.825316653672516 1102 86-87 0.697239549478244 1102 88-89 0.7403987408550314 1102 90-91 0.7302170975378459 1102 92-93 0.589927172986549 1102 94-95 0.5828107587707265 1102 96-97 0.7079009830472671 1102 98-99 0.6724826110560329 1102 100-101 0.7347963400902167 1103 1 0.3402550660146204 1103 2 0.3035802130489458 1103 3 0.20706618765709806 1103 4 0.017791821692071608 1103 5 0.012072943631835642 1103 6 0.0742970798860938 1103 7 0.12892375915486554 1103 8 0.14823876377960232 1103 9 0.1822326490268793 1103 10-11 0.2091915318511326 1103 12-13 0.12496098392287536 1103 14-15 0.2687011190760984 1103 16-17 0.1421227265507028 1103 18-19 0.14052157004613264 1103 20-21 0.26997424964426386 1103 22-23 0.2258742033666934 1103 24-25 0.24507482248309742 1103 26-27 0.18927329743063837 1103 28-29 0.16266216699565206 1103 30-31 0.2554359977993528 1103 32-33 0.1953113853822046 1103 34-35 0.17594094488975287 1103 36-37 0.2640399613531841 1103 38-39 0.3304764911617539 1103 40-41 0.37888771323749637 1103 42-43 0.33990849468376894 1103 44-45 0.2542456737466381 1103 46-47 0.39111155844639 1103 48-49 0.44926332165065475 1103 50-51 0.30866321747348024 1103 52-53 0.3086613706468526 1103 54-55 0.29991918518915384 1103 56-57 0.17674554611205195 1103 58-59 0.3160754783704931 1103 60-61 0.2534593701468211 1103 62-63 0.32520279549792974 1103 64-65 0.23938215730380108 1103 66-67 0.29300596065475304 1103 68-69 0.3603358209078209 1103 70-71 0.41031018788527973 1103 72-73 0.4563416743996953 1103 74-75 0.20879065777406325 1103 76-77 0.33957835543441917 1103 78-79 0.3452139222094921 1103 80-81 0.41579600268141803 1103 82-83 0.2566838687174027 1103 84-85 0.4666901008820794 1103 86-87 0.5091704548872151 1103 88-89 0.5967273938211441 1103 90-91 0.6234697439942316 1103 92-93 0.6522970478614631 1103 94-95 0.7290815338120282 1103 96-97 0.805294358445753 1103 98-99 0.7383487553167711 1103 100-101 0.6546105677567517 1104 1 0.3524863731635506 1104 2 0.2563090957999279 1104 3 0.2635921401956267 1104 4 0.2433870316489859 1104 5 0.21445656915351918 1104 6 0.21629994324189283 1104 7 0.2544893394571375 1104 8 0.27458687085830036 1104 9 0.12497608491877088 1104 10-11 0.36575703280333016 1104 12-13 0.17285318431726182 1104 14-15 0.32411279400417925 1104 16-17 0.4427793061911629 1104 18-19 0.31305542889079874 1104 20-21 0.2828175653292391 1104 22-23 0.19869321598167033 1104 24-25 0.47616586909366987 1104 26-27 0.42088708374595996 1104 28-29 0.5183598398437468 1104 30-31 0.3615110055655322 1104 32-33 0.5325806370603772 1104 34-35 0.41379574101070915 1104 36-37 0.5769075885496662 1104 38-39 0.4910149826999941 1104 40-41 0.5476003174055748 1104 42-43 0.6644878548858202 1104 44-45 0.6246121360165233 1104 46-47 0.6779483522307501 1104 48-49 0.7614024476518253 1104 50-51 0.6991761566001529 1104 52-53 0.5320951352844077 1104 54-55 0.6432344480911354 1104 56-57 0.5265609727790732 1104 58-59 0.7164227615590875 1104 60-61 0.6767369248814887 1104 62-63 0.4581055139976442 1104 64-65 0.6364541396374364 1104 66-67 0.764799542415723 1104 68-69 0.7525796890043637 1104 70-71 0.723335249001714 1104 72-73 0.6655823279741568 1104 74-75 0.5967743133474741 1104 76-77 0.7009043964398352 1104 78-79 0.6586359447246544 1104 80-81 0.6421591778323545 1104 82-83 0.8007073310143014 1104 84-85 0.6402770364835924 1104 86-87 0.6313712544658152 1104 88-89 0.7132495983349827 1104 90-91 0.5860519641754891 1104 92-93 0.7591379104474001 1104 94-95 0.8199428535219084 1104 96-97 0.7924041924521568 1104 98-99 0.8885191891421869 1104 100-101 0.8752126322092657 1105 1 0.380024710261992 1105 2 0.2884868121022066 1105 3 0.3709918543026589 1105 4 0.2929257261136371 1105 5 0.2908623425322858 1105 6 0.22531386776104512 1105 7 0.24075945726035997 1105 8 0.21521063443502442 1105 9 0.20284323083905065 1105 10-11 0.2956165352347 1105 12-13 0.21317793288461218 1105 14-15 0.3193569517047763 1105 16-17 0.2801673101732476 1105 18-19 0.33762295993698643 1105 20-21 0.3355653764611972 1105 22-23 0.30448099310002164 1105 24-25 0.3395402995412766 1105 26-27 0.2828789137800385 1105 28-29 0.3614441655064766 1105 30-31 0.2625309351133325 1105 32-33 0.3114449384094371 1105 34-35 0.41716798578104175 1105 36-37 0.41662684867397104 1105 38-39 0.22923166946851126 1105 40-41 0.14566782771175468 1105 42-43 0.08056063953439008 1105 44-45 0.21938996271943267 1105 46-47 0.08525030912982601 1105 48-49 0.1235137321280888 1105 50-51 0.26708610669651733 1105 52-53 0.10254334270996424 1105 54-55 0.08434600059206332 1105 56-57 0.33722887267824575 1105 58-59 0.2450718853737115 1105 60-61 0.016688911773812265 1105 62-63 0.036721493691580065 1105 64-65 0.03815529852500532 1105 66-67 0.13936240696116897 1105 68-69 0.18083084406300998 1105 70-71 0.22903954760671041 1105 72-73 0.2138750268548364 1105 74-75 0.2982548703920571 1105 76-77 0.13616407723523238 1105 78-79 0.08668308994296581 1105 80-81 0.14293353776394468 1105 82-83 0.166770583238268 1105 84-85 0.30493571186744006 1105 86-87 0.5363612992642217 1105 88-89 0.41561858532940477 1105 90-91 0.38201340691393426 1105 92-93 0.7086022998617025 1105 94-95 0.8759596082538188 1105 96-97 1.0649836745264096 1105 98-99 1.0746786670046262 1105 100-101 0.7884887071795923 1106 1 0.07632746539443502 1106 2 0.03274719249783686 1106 3 -0.006870765996893624 1106 4 0.05720359904847783 1106 5 0.047230612247147974 1106 6 0.05362586073334796 1106 7 0.10793157472033954 1106 8 0.02134061527452502 1106 9 0.017879759264047834 1106 10-11 -0.509279918672533 1106 12-13 -0.30361693699306613 1106 14-15 -0.1637452247397988 1106 16-17 -0.0765942157498003 1106 18-19 -0.05442426148321289 1106 20-21 -0.02782391229185066 1106 22-23 -0.29506198459642263 1106 24-25 -0.18707753985788855 1106 26-27 -0.10677314880199162 1106 28-29 -0.3440383383116341 1106 30-31 -0.16693889370748138 1106 32-33 -0.1350791575441619 1106 34-35 -0.43791388060617464 1106 36-37 -0.43807828777511304 1106 38-39 -0.2310784415795908 1106 40-41 0.03948194129976201 1106 42-43 -0.4520461514205607 1106 44-45 -0.5134659492948543 1106 46-47 -0.21047598209525376 1106 48-49 -0.15355167777517664 1106 50-51 -0.17231482176543977 1106 52-53 -0.09124615402101455 1106 54-55 -0.5103769428171603 1106 56-57 -0.3798737846002709 1106 58-59 -0.1580352834615013 1106 60-61 -0.43044562088360294 1106 62-63 -0.22151834734946618 1106 64-65 -0.20292463762454105 1106 66-67 -0.4341909980064571 1106 68-69 -0.7967026312198584 1106 70-71 -0.5471769258252976 1106 72-73 -0.40400961824639126 1106 74-75 -0.4556762537892496 1106 76-77 -0.29053654689028363 1106 78-79 -0.029311578705318198 1106 80-81 -0.36056017707699084 1106 82-83 -0.157232309434157 1106 84-85 -0.18490005688594735 1106 86-87 0.013137480269442392 1106 88-89 -0.26431127580007896 1106 90-91 -0.33510727205119295 1106 92-93 -0.22928806616642206 1106 94-95 -0.2446729606149063 1106 96-97 -0.12914919285046267 1106 98-99 -0.2816683601264458 1106 100-101 -0.26609802295408613 1107 1 0.05969980499251193 1107 2 0.11202564537389748 1107 3 0.03166199470086184 1107 4 0.014094890432758689 1107 5 0.14827247937704868 1107 6 0.10467190906216928 1107 7 -0.011834760164845193 1107 8 0.0025810911789463375 1107 9 0.020777384890813266 1107 10-11 0.16698920300912334 1107 12-13 0.03525590672248313 1107 14-15 0.11828540646786223 1107 16-17 0.09511959013082816 1107 18-19 0.17528076497519152 1107 20-21 0.2222718535960695 1107 22-23 0.25154085518624214 1107 24-25 0.16664914995813263 1107 26-27 0.2777828535190281 1107 28-29 0.2596842433395139 1107 30-31 0.3059119791771252 1107 32-33 0.23091962497834828 1107 34-35 0.285484656302863 1107 36-37 0.21274942528435759 1107 38-39 0.25921113474628754 1107 40-41 0.27626761815553635 1107 42-43 0.2346975126023949 1107 44-45 0.18903261832498686 1107 46-47 0.28755885441255913 1107 48-49 0.023314679787077353 1107 50-51 -0.05879673178092304 1107 52-53 0.058959856753695306 1107 54-55 0.10043724433768375 1107 56-57 -0.0015061392652668815 1107 58-59 -0.10225955055575753 1107 60-61 0.02044647168412439 1107 62-63 0.12146065280718688 1107 64-65 0.18973157032382204 1107 66-67 0.18318967988822266 1107 68-69 0.16630270501244127 1107 70-71 0.1979063311570357 1107 72-73 0.2606739307260355 1107 74-75 0.27850849823644097 1107 76-77 0.21604984562606333 1107 78-79 0.18959061739181493 1107 80-81 0.21946979609395356 1107 82-83 0.22729959165292257 1107 84-85 0.07738636875133764 1107 86-87 0.24172453812591144 1107 88-89 0.2548597951236431 1107 90-91 0.2448427288434729 1107 92-93 0.082188977956001 1107 94-95 0.11813989439900752 1107 96-97 -0.034079319356379756 1107 98-99 0.22119147038345233 1107 100-101 0.42557781252797255 1108 1 0.25926158324758575 1108 2 0.199698951742306 1108 3 0.18270978725445275 1108 4 0.15226308206915462 1108 5 0.12505830984979838 1108 6 0.09895300446638089 1108 7 0.06271262877286432 1108 8 0.17577234504797445 1108 9 0.2066462529859976 1108 10-11 0.2139900760206217 1108 12-13 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8 1649.0 9 2146.0 10 2728.0 11 3644.0 12 5079.0 13 7084.0 14 8238.0 15 10724.0 16 14845.0 17 22059.0 18 18911.0 19 11847.0 20 10965.0 21 11106.0 22 11311.0 23 12341.0 24 13625.0 25 15218.0 26 17601.0 27 20340.0 28 24078.0 29 28805.0 30 34936.0 31 43385.0 32 54673.0 33 71911.0 34 102006.0 35 165226.0 36 230002.0 37 318342.0 38 263721.0 39 33865.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.53374991761424 18.791424347897046 14.474472966483901 21.20035276800482 2 15.736336253765106 20.751896614215546 40.18672432540525 23.32504280661409 3 19.16449125324437 26.328825014264922 29.950521193016094 24.556162539474613 4 12.304837407215736 17.01830309485058 36.7513819199124 33.92547757802129 5 12.91176557000038 38.52551091529992 33.04207530409876 15.520648210600935 6 30.587085506977235 36.4298653200548 17.24339746872485 15.739651704243116 7 27.8600475283864 34.95642814550537 19.928294891010985 17.25522943509725 8 27.270312765595783 33.68435722070572 19.279129295777466 19.766200717921027 9 28.014600793532257 16.467950308307717 20.11621141779055 35.40123748036948 10-11 25.367975569453815 27.211955490700245 25.75480505225495 21.665263887590985 12-13 26.80909667878682 25.197668929164337 27.066433642954735 20.926800749094117 14-15 23.336675798293438 25.692910484955316 26.896854072812825 24.073559643938424 16-17 21.19206870819641 29.53638092000085 27.17734909458627 22.094201277216474 18-19 21.085803032386284 26.63593865230054 31.4497268852418 20.828531430071372 20-21 22.25017002040245 25.46364938792655 31.70680481657799 20.579375775093013 22-23 21.796944727085453 25.705380290352174 31.72226916701875 20.775405815543614 24-25 21.38297742653708 26.06108591556824 31.84484616305519 20.71109049483949 26-27 21.024518875890575 26.357057184876286 31.88391394486456 20.734509994368576 28-29 20.66095634948041 26.679258573366553 32.016204058889876 20.64358101826316 30-31 20.840875509096865 26.238114822750536 31.89230898085621 21.028700687296393 32-33 20.669332679955897 26.460598590404853 31.752237025999253 21.117831703639997 34-35 21.431169871844958 26.432360601778033 31.384953299009478 20.751516227367535 36-37 21.418037528364405 26.13697481118782 31.413402969430347 21.031584691017425 38-39 20.97919030032489 26.384394740385662 31.489421129373053 21.14699382991639 40-41 21.080912027529415 26.422859863733887 31.52313796358176 20.973090145154934 42-43 21.127575968799217 26.38778712958366 31.047065178778695 21.437571722838427 44-45 20.947350336576758 26.585565464569633 31.045902509878644 21.421181688974965 46-47 21.368024123777747 26.939728354466837 30.417345978344706 21.27490154341071 48-49 21.308444731008663 26.9966924324554 30.22497582114394 21.469887015391997 50-51 21.213407655091064 27.275322877306625 29.92394140226714 21.587328065335175 52-53 21.555814969813156 27.390511581645676 29.673634719665476 21.380038728875693 54-55 21.107810665268048 27.276586559441917 29.73712464283494 21.878478132455093 56-57 21.33545126263573 27.52196024040684 29.542770926266073 21.599817570691357 58-59 21.570858103870076 27.068449145744466 29.52026373593421 21.84042901445125 60-61 21.365769846538328 27.190971923932995 30.03797102581124 21.405287203717442 62-63 21.19104245811382 27.237683098780725 30.003117372511024 21.56815707059443 64-65 21.088404137483813 27.275919092037586 29.887679196942806 21.747997573535795 66-67 21.192706428336944 27.388906467130546 29.552664811112333 21.865722293420177 68-69 21.16104833817796 27.252969402333793 29.77814052599324 21.80784173349501 70-71 21.234469235902655 27.506042345154754 29.48745666632508 21.772031752617515 72-73 21.326061127889574 27.440349680695487 29.369516908408766 21.864072283006177 74-75 21.078573789409823 27.954084951087804 29.13101421349673 21.836327046005643 76-77 21.237930456061722 27.592326095641734 29.262281486238745 21.9074619620578 78-79 21.37159042069386 27.721697618167095 29.116264280502552 21.790447680636486 80-81 21.41568247362271 28.065642508268724 28.96102886032679 21.557646157781782 82-83 21.53052401675794 27.95259996726379 28.817329449969197 21.69954656600907 84-85 21.35161542022416 27.852715856337955 28.770578904986415 22.02508981845147 86-87 21.58133352322628 27.696261232730045 28.749417515875425 21.972987728168246 88-89 21.54729412235186 27.744681223318636 28.601984043669955 22.106040610659548 90-91 21.62204640265819 27.75935460103607 28.70886773651979 21.909731259785957 92-93 21.909996875976255 27.504592314901593 28.677694470477977 21.907716338644175 94-95 21.723918664510634 27.36381461257841 28.773671423996 22.138595298914954 96-97 21.818092064358943 27.220516678674556 28.75341256247903 22.207978694487473 98-99 21.834212699169413 27.383105366761 28.306197381365276 22.476484552704314 100-101 22.170401550595177 26.88866171073492 28.605740655699552 22.335196082970356 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5792.0 1 3665.0 2 2267.0 3 2890.0 4 2125.5 5 1949.5 6 3967.5 7 8181.0 8 12391.0 9 12876.0 10 9020.5 11 5478.0 12 4798.0 13 4791.5 14 4663.5 15 4438.0 16 4321.0 17 4392.5 18 4543.0 19 4634.5 20 4732.5 21 5107.5 22 5812.5 23 6719.5 24 7569.5 25 8636.0 26 10245.0 27 11918.5 28 13538.0 29 15517.0 30 17846.0 31 20296.0 32 23515.5 33 26242.5 34 28200.0 35 30064.5 36 32678.0 37 35922.5 38 38394.0 39 39028.0 40 39409.5 41 42036.5 42 45158.5 43 48384.0 44 51045.0 45 53602.5 46 54205.0 47 55689.0 48 61817.5 49 61833.5 50 56257.0 51 52404.0 52 50276.5 53 50705.5 54 51132.5 55 48474.0 56 44049.5 57 40401.0 58 37460.5 59 33255.0 60 28332.0 61 23923.0 62 19814.0 63 15461.5 64 11786.5 65 9375.5 66 7164.5 67 5720.5 68 4162.5 69 2670.5 70 1985.5 71 1556.0 72 1316.0 73 1153.0 74 891.5 75 708.5 76 492.5 77 266.5 78 148.5 79 90.0 80 48.5 81 33.5 82 24.0 83 16.0 84 11.0 85 6.0 86 4.5 87 7.0 88 4.5 89 1.5 90 1.5 91 3.0 92 2.5 93 1.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4959230007713664 2 0.05215311154207694 3 0.060959804628822875 4 0.06914190955338823 5 0.08294530793757866 6 0.11130161050057617 7 0.07301435615890771 8 0.05490129945567142 9 0.0542142524772728 10-11 0.07835458494555152 12-13 0.11180128103032064 14-15 0.05861759902064576 16-17 0.06327078082889095 18-19 0.062146522136965934 20-21 0.0778861438239161 22-23 0.06664355690466599 24-25 0.09090880700538083 26-27 0.06895453310473407 28-29 0.06779904500470002 30-31 0.07263960326159938 32-33 0.07263960326159938 34-35 0.07470074419679525 36-37 0.06986018593989587 38-39 0.04194109509042475 40-41 0.045626165247290074 42-43 0.04437698892292894 44-45 0.023734350162861365 46-47 0.029761625927903785 48-49 0.030167608233321156 50-51 0.02791909084947113 52-53 0.027513108544053765 54-55 0.026826061565655143 56-57 0.027169585054854454 58-59 0.022672550287154408 60-61 0.030760966987392686 62-63 0.021735668043883563 64-65 0.023234679633116914 66-67 0.01720740386807449 68-69 0.01948715066003354 70-71 0.0426593714769324 72-73 0.03894307191195805 74-75 0.03335300786044202 76-77 0.02395295601962456 78-79 0.016926339195093235 80-81 0.0183316625599995 82-83 0.02379680897907942 84-85 0.020361574087086328 86-87 0.009556198881362603 88-89 0.04290920674180462 90-91 0.054120564252945716 92-93 0.0346646430010212 94-95 0.05574449347461517 96-97 0.044845430044564365 98-99 0.035258001755092735 100-101 0.16870126260496984 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1601055.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.807278063926546 #Duplication Level Percentage of deduplicated Percentage of total 1 73.48008733671901 34.39402880131414 2 13.832791990310431 12.949506821818325 3 5.099262844935007 7.160478417117661 4 2.4372046424950713 4.56315661599838 5 1.3105070504178367 3.0670633956821938 6 0.8014735886524885 2.2508878274970066 7 0.5482847169060606 1.796460064169626 8 0.3935577459223848 1.4737093478080976 9 0.28009863160142073 1.179958908122372 >10 1.5889059448469511 13.809748453236967 >50 0.1455098334737196 4.700503954312929 >100 0.07576794527594563 6.405217654610122 >500 0.004676948888310264 1.5403502258506463 >1k 0.0013362711109457897 1.3868593640226734 >5k 1.3362711109457897E-4 0.32305635751486533 >10k+ 4.0088133328373694E-4 2.9990137909240584 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20746 1.2957706012597943 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15994 0.9989663065915911 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11208 0.7000384121719742 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5165 0.32259978576626036 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3805 0.23765579570970394 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3362 0.209986540125105 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2615 0.16332980441021702 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2597 0.16220554571829202 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2355 0.1470905121935224 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2080 0.12991433773355693 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1767 0.11036472825730534 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 6.245881621805621E-5 0.0 16-17 0.0 0.0 0.0 6.245881621805621E-5 0.0 18-19 0.0 0.0 0.0 1.2491763243611243E-4 0.0 20-21 0.0 0.0 0.0 3.122940810902811E-4 0.0 22-23 0.0 0.0 0.0 6.245881621805622E-4 0.0 24-25 0.0 0.0 0.0 0.0010305704675979276 0.0 26-27 0.0 0.0 0.0 0.003154170219011839 0.0 28-29 0.0 0.0 0.0 0.010212016451652192 0.0 30-31 0.0 0.0 0.0 0.027794173217035017 0.0 32-33 0.0 0.0 0.0 0.060991034036931895 0.0 34-35 0.0 0.0 0.0 0.11045841648163243 0.0 36-37 0.0 0.0 0.0 0.1875325956947138 0.0 38-39 0.0 0.0 0.0 0.299896006070997 0.0 40-41 0.0 0.0 0.0 0.42172192710431555 0.0 42-43 0.0 0.0 0.0 0.535553119661723 0.0 44-45 0.0 0.0 0.0 0.674399068114462 0.0 46-47 0.0 0.0 0.0 0.8328883142677797 0.0 48-49 0.0 0.0 0.0 1.002307853259257 0.0 50-51 0.0 0.0 0.0 1.1781606503211943 0.0 52-53 0.0 0.0 0.0 1.3447070837666413 0.0 54-55 0.0 0.0 0.0 1.5236828216394813 0.0 56-57 0.0 0.0 0.0 1.724331768739987 0.0 58-59 0.0 0.0 0.0 1.9404392728544617 0.0 60-61 0.0 0.0 0.0 2.172910986818067 0.0 62-63 0.0 0.0 0.0 2.3991992779760842 0.0 64-65 0.0 0.0 0.0 2.6249566691962487 0.0 66-67 0.0 0.0 0.0 2.876415863290143 0.0 68-69 0.0 0.0 0.0 3.1558566070497265 0.0 70-71 0.0 0.0 0.0 3.453566554553092 0.0 72-73 0.0 0.0 0.0 3.7467170084725385 0.0 74-75 0.0 0.0 0.0 4.039523938902786 0.0 76-77 0.0 0.0 0.0 4.342261821111704 0.0 78-79 0.0 3.122940810902811E-5 0.0 4.677696893610776 0.0 80-81 0.0 6.245881621805621E-5 0.0 5.038927457207903 0.0 82-83 0.0 6.245881621805621E-5 0.0 5.394286892080535 0.0 84-85 0.0 6.245881621805621E-5 0.0 5.748272232996367 0.0 86-87 0.0 6.245881621805621E-5 0.0 6.115405154726103 0.0 88-89 0.0 6.245881621805621E-5 0.0 6.506366114843026 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9265 0.0 37.91234 1 GTATCAA 14345 0.0 33.6023 1 GTACATG 11395 0.0 27.34935 1 ATCAACG 17765 0.0 27.176817 3 ACCGTAT 70 0.008355494 27.167482 8 TATCAAC 17825 0.0 27.138655 2 TCAACGC 17875 0.0 27.036161 4 CAACGCA 18020 0.0 26.844982 5 AACGCAG 18345 0.0 26.369396 6 TACATGG 11490 0.0 26.303118 2 ACATGGG 11645 0.0 24.810423 3 GAGTACT 11300 0.0 24.485266 12-13 ACGCAGA 20520 0.0 23.493517 7 CATGGGA 7520 0.0 23.317322 4 CGCAGAG 20680 0.0 23.26577 8 ATGGGAG 2510 0.0 23.097034 5 GTACTTT 11855 0.0 23.040602 14-15 TATCTCG 105 0.002191413 22.628242 5 GCAGAGT 21515 0.0 22.053455 9 AGTACTT 11310 0.0 22.025661 12-13 >>END_MODULE