FastQCFastQC Report
Thu 26 May 2016
SRR938021_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938021_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1601055
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200331.2512374652963203No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158710.9912838721967703No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113630.7097195286857728No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49810.31110736358213803No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39490.246649865245104No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34100.2129845633035717No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25290.1579583462154642No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23380.14602871231781542No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20840.13016417299842917No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19490.12173223280899158No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17810.11123915168435813No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90950.036.8782731
GTATCAA142750.034.0461541
TATCACG753.07587E-431.6721322
ATACCGT1859.094947E-1230.8093936
TATCAAC177800.027.2277032
ATCAACG178400.027.1318913
TCAACGC180100.026.9268574
CAACGCA180700.026.7585935
AATACCG908.951494E-426.3876745
AACGCAG185350.026.164166
GTACATG116150.025.1550081
TACATGG117650.024.067052
GAGTACT113550.023.11112-13
ACGCAGA210050.022.9065887
CGCAGAG210600.022.7791028
AGTACTT114950.022.72622712-13
ACATGGG120400.022.5668163
ACCGTCG1903.290079E-722.4989648
CGTTTAA1302.938775E-421.9268631
GCAGAGT217750.021.8130049
Produced by FastQC (version 0.11.5)