FastQCFastQC Report
Thu 26 May 2016
SRR938020_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938020_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1674092
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211441.2630130243738098No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161420.9642241883958588No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112270.6706321994251212No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53240.3180231432920055No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39080.23343997820908288No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33110.19777885564234224No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27350.16337214442217035No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26520.1584142329095414No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24400.14575065169656148No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21560.12878623158106006No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17640.10537055311177641No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92150.040.359371
GTATCAA148000.035.8758161
TAGGACG702.0374768E-433.974494
GTACATG119300.028.8593461
TATCAAC186450.028.290272
ATCAACG186750.028.2711413
TACATGG119850.028.1368942
TCAACGC188800.027.9137884
CAACGCA189750.027.7489685
ATACCGT2600.027.435186
ACATGGG119450.027.1966573
AACGCAG194500.027.0656176
CATGGGA78400.024.6921774
GAGTACT119950.024.53297812-13
ACGCAGA215100.024.2930587
CGCAGAG216750.024.0840288
CATGGGG39900.023.8417474
GTAAGCG1500.023.75154794-95
TACCGTC2653.092282E-1123.3250397
ATGGGAG28000.023.2725265
Produced by FastQC (version 0.11.5)