##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938020_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1674092 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17445875137089 33.0 31.0 34.0 31.0 34.0 2 32.26009562198493 34.0 31.0 34.0 31.0 34.0 3 32.26428057717258 34.0 31.0 34.0 30.0 34.0 4 35.782307662900244 37.0 35.0 37.0 35.0 37.0 5 35.81289678225569 37.0 35.0 37.0 35.0 37.0 6 35.868799325246165 37.0 37.0 37.0 35.0 37.0 7 35.81320620372118 37.0 36.0 37.0 35.0 37.0 8 35.81529091591143 37.0 36.0 37.0 35.0 37.0 9 37.46788229081795 39.0 38.0 39.0 35.0 39.0 10-11 37.404358900227706 39.0 37.5 39.0 35.0 39.0 12-13 37.26286787106085 39.0 37.0 39.0 34.0 39.0 14-15 38.446028055805776 40.0 38.0 41.0 34.0 41.0 16-17 38.31002477761079 40.0 38.0 41.0 34.0 41.0 18-19 38.52344255871242 40.0 38.0 41.0 34.0 41.0 20-21 38.55576605108919 40.0 38.0 41.0 34.0 41.0 22-23 38.448940679484764 40.0 38.0 41.0 34.0 41.0 24-25 38.32805544737087 40.0 38.0 41.0 34.0 41.0 26-27 38.158384963311455 40.0 38.0 41.0 34.0 41.0 28-29 37.9857552631516 40.0 38.0 41.0 33.0 41.0 30-31 37.72326998755146 40.0 37.0 41.0 33.0 41.0 32-33 37.38865247549119 40.0 36.5 41.0 33.0 41.0 34-35 37.141522986789255 40.0 36.0 41.0 32.0 41.0 36-37 36.88926594237354 39.0 35.0 41.0 31.0 41.0 38-39 36.61014567897105 39.0 35.0 41.0 31.0 41.0 40-41 36.4730618150018 39.0 35.0 41.0 30.0 41.0 42-43 36.21747699648526 39.0 35.0 41.0 30.0 41.0 44-45 35.83674314195397 39.0 35.0 41.0 29.5 41.0 46-47 35.35806036944206 39.0 35.0 40.5 26.5 41.0 48-49 34.8879219302165 38.0 35.0 40.0 24.5 41.0 50-51 34.11952628648844 37.5 33.5 39.5 23.5 40.5 52-53 34.187281224687766 38.0 34.0 40.0 23.0 40.5 54-55 34.286287432231916 38.0 34.0 40.0 23.0 41.0 56-57 34.01419515773327 38.0 34.0 40.0 22.0 41.0 58-59 33.64779862755452 37.5 33.5 40.0 20.0 41.0 60-61 33.198473859262215 37.0 33.0 40.0 17.5 41.0 62-63 32.721759019217586 36.0 32.5 39.5 13.5 41.0 64-65 32.24717608112338 35.5 32.0 39.0 9.0 40.5 66-67 31.75045726280276 35.0 31.0 39.0 6.5 40.0 68-69 31.15014019540145 35.0 31.0 38.0 2.0 40.0 70-71 30.628828045292614 35.0 30.0 37.5 2.0 40.0 72-73 30.004406567858876 35.0 29.0 37.0 2.0 39.0 74-75 29.40086028724825 34.0 29.0 36.0 2.0 39.0 76-77 28.72842173548407 34.0 27.5 36.0 2.0 38.5 78-79 28.03657594684163 34.0 26.5 35.0 2.0 37.0 80-81 27.36390801700265 33.0 25.5 35.0 2.0 37.0 82-83 26.68601008785658 33.0 24.5 35.0 2.0 36.0 84-85 26.01356675738251 32.0 22.5 35.0 2.0 36.0 86-87 25.3283409752869 32.0 19.5 35.0 2.0 35.0 88-89 24.513225975633357 31.0 15.0 34.0 2.0 35.0 90-91 23.686269631537574 31.0 4.5 34.0 2.0 35.0 92-93 22.803880850036915 30.0 2.0 34.0 2.0 35.0 94-95 21.77960679580334 29.5 2.0 34.0 2.0 35.0 96-97 20.567948475949947 29.0 2.0 34.0 2.0 35.0 98-99 19.06449615672257 26.5 2.0 34.0 2.0 35.0 100-101 14.922790981618691 10.5 2.0 29.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.08855297129709072 1101 2 0.09105902213248385 1101 3 0.20035032757214566 1101 4 0.0031469676341941977 1101 5 -0.03780051991537192 1101 6 -0.025478043698484498 1101 7 0.08352784548511494 1101 8 -0.007047520073754754 1101 9 0.08371102249729034 1101 10-11 0.03124978441012871 1101 12-13 0.07208385359393787 1101 14-15 0.1781181893458097 1101 16-17 0.1819498158231312 1101 18-19 0.17243229601335486 1101 20-21 0.08026591333427291 1101 22-23 0.051048038181946254 1101 24-25 0.1435249010363222 1101 26-27 0.1422298262746935 1101 28-29 0.05551922294025502 1101 30-31 0.06692530652445328 1101 32-33 0.06462254792165822 1101 34-35 0.06605187509232735 1101 36-37 -0.022297607410266096 1101 38-39 0.04750831445331727 1101 40-41 0.1083885077887814 1101 42-43 0.09223984558803977 1101 44-45 0.21366113012573607 1101 46-47 0.031161994873038168 1101 48-49 0.21831728265457429 1101 50-51 0.03127327681858816 1101 52-53 0.03817289844329963 1101 54-55 -0.14130830804071337 1101 56-57 -0.17959705164995654 1101 58-59 -0.057551686560486814 1101 60-61 -0.3307009154208629 1101 62-63 -0.4212280338780161 1101 64-65 -0.4493034534323286 1101 66-67 -0.4163258108202079 1101 68-69 -0.51390705919356 1101 70-71 -0.6335880680941024 1101 72-73 -0.6099241420945631 1101 74-75 -0.5692531494265225 1101 76-77 -0.768507599530281 1101 78-79 -0.7245620130238564 1101 80-81 -0.9174430294921088 1101 82-83 -1.1097778354263141 1101 84-85 -1.2586139234211338 1101 86-87 -1.0147663819182 1101 88-89 -0.994665985847508 1101 90-91 -0.91148156638506 1101 92-93 -1.2275178059782235 1101 94-95 -1.5394782484226752 1101 96-97 -1.6591125682583474 1101 98-99 -1.6502033689656805 1101 100-101 -1.6672443093725544 1102 1 0.21452576283751768 1102 2 0.14370115365984049 1102 3 0.17902688552378976 1102 4 0.14471041443511723 1102 5 0.11128535307221199 1102 6 0.15415793919226672 1102 7 0.0673653967237513 1102 8 0.1774561334639344 1102 9 0.23211000069488108 1102 10-11 0.20201554214791173 1102 12-13 0.27050919369968796 1102 14-15 0.17955695545999362 1102 16-17 0.23420208814535926 1102 18-19 0.14949372297230212 1102 20-21 0.27519180295585244 1102 22-23 0.2877872557902137 1102 24-25 0.24910805614830878 1102 26-27 0.2099576525682778 1102 28-29 0.027974741861420682 1102 30-31 0.044523254008552726 1102 32-33 0.015743485960030057 1102 34-35 -0.07064761400646802 1102 36-37 0.08944903608762189 1102 38-39 -0.04915634722314621 1102 40-41 0.08565785965713246 1102 42-43 0.057754014185434244 1102 44-45 -0.20909105308581388 1102 46-47 -0.144692353727919 1102 48-49 -0.18852121909792885 1102 50-51 -0.23758291340300275 1102 52-53 -0.25033033924457726 1102 54-55 -0.2985218172191182 1102 56-57 -0.1220074211622233 1102 58-59 -0.007616291800189856 1102 60-61 -0.06556509589968584 1102 62-63 -0.16162078918009826 1102 64-65 -0.15016819827875594 1102 66-67 -0.14381236995999203 1102 68-69 -0.2209538571499543 1102 70-71 -0.5504608031977689 1102 72-73 -0.5492861839171717 1102 74-75 -0.6005291400401944 1102 76-77 -0.8463977557709974 1102 78-79 -0.8716633170100714 1102 80-81 -0.5598660230167312 1102 82-83 -0.29353704352944376 1102 84-85 -0.42331087286567737 1102 86-87 -0.5216668898490475 1102 88-89 -0.6764276296424363 1102 90-91 -0.45779498348242953 1102 92-93 -0.6629923945839415 1102 94-95 -1.0117555459929264 1102 96-97 -1.3114157079244784 1102 98-99 -1.3892000570189111 1102 100-101 -1.5583492074893481 1103 1 0.17470882683767996 1103 2 0.19523563036922553 1103 3 0.24843306264389042 1103 4 0.12141851300486906 1103 5 0.13648890084014909 1103 6 0.14605601567875937 1103 7 0.21491431010372963 1103 8 0.21923631219384276 1103 9 0.2054166070969643 1103 10-11 0.28911025248576294 1103 12-13 0.20803305703115882 1103 14-15 0.2696681198873705 1103 16-17 0.24387254198722275 1103 18-19 0.21925333538020908 1103 20-21 0.2702566080246811 1103 22-23 0.3001813541948479 1103 24-25 0.10369587836778038 1103 26-27 0.12170976286072488 1103 28-29 0.15843170531054085 1103 30-31 0.18595219029732846 1103 32-33 0.23422131499022214 1103 34-35 0.08933216020771795 1103 36-37 0.18802845841441496 1103 38-39 0.145616809237751 1103 40-41 0.21304032307529042 1103 42-43 0.18994913110286404 1103 44-45 0.1910467092818422 1103 46-47 0.2416994847547329 1103 48-49 0.2093488818246172 1103 50-51 0.3247526230962876 1103 52-53 0.08989756202056753 1103 54-55 0.10249566363862073 1103 56-57 0.26565626839259693 1103 58-59 0.2086022601986457 1103 60-61 0.20680774479129838 1103 62-63 0.42268578110450505 1103 64-65 0.24004435305965188 1103 66-67 0.022493766914916336 1103 68-69 -0.04805370132885045 1103 70-71 -0.08440800460944686 1103 72-73 -0.05392018490782391 1103 74-75 -0.13622287933394261 1103 76-77 -0.009487502535904468 1103 78-79 -0.13482233193174764 1103 80-81 -0.24672042315707543 1103 82-83 -0.40084270571754743 1103 84-85 -0.40597384007100956 1103 86-87 -0.7305103825120405 1103 88-89 -0.7616693845389335 1103 90-91 -0.5684337726182953 1103 92-93 -0.6673916319892612 1103 94-95 -0.6935663987780956 1103 96-97 -0.7711505282658422 1103 98-99 -0.9068350589795742 1103 100-101 -1.0585730068412982 1104 1 0.238793514217015 1104 2 0.13651695484978177 1104 3 0.1869488981787768 1104 4 0.181666034299937 1104 5 0.23965568671584947 1104 6 0.1495463918575055 1104 7 0.20298738644326875 1104 8 0.19591750068779845 1104 9 0.20543908422046542 1104 10-11 0.14960799233071498 1104 12-13 0.20309872635574777 1104 14-15 0.3157839525323425 1104 16-17 0.19706433366282283 1104 18-19 0.33208789331539634 1104 20-21 0.29855731340497726 1104 22-23 0.45025006997242656 1104 24-25 0.4632979449787271 1104 26-27 0.2856227812092911 1104 28-29 0.18219765102346486 1104 30-31 0.3018562804113287 1104 32-33 0.34345130493171894 1104 34-35 0.2680485719597243 1104 36-37 0.2835012446385008 1104 38-39 0.29345868427939337 1104 40-41 0.27728455789380035 1104 42-43 0.369780970108863 1104 44-45 0.4251876938280219 1104 46-47 0.11563493206153197 1104 48-49 0.23036479161428502 1104 50-51 0.14936503319782446 1104 52-53 0.07158332220090102 1104 54-55 0.03384350434966876 1104 56-57 0.08009918492781765 1104 58-59 0.03897222562402192 1104 60-61 0.030563615936372912 1104 62-63 0.19533650025204707 1104 64-65 0.15544146362542222 1104 66-67 0.051208693571258124 1104 68-69 0.09910262144585502 1104 70-71 -0.12381341244481092 1104 72-73 -0.14412308911273186 1104 74-75 -0.23877395257645162 1104 76-77 -0.17124412184809046 1104 78-79 -0.3065329052509185 1104 80-81 -0.17363986916020124 1104 82-83 -0.4926561359593933 1104 84-85 -0.23195273886856427 1104 86-87 -0.13001295574130367 1104 88-89 -0.002685960614766003 1104 90-91 0.119721425497481 1104 92-93 -0.23901378191007083 1104 94-95 -0.39810902543869275 1104 96-97 -0.9723270049927848 1104 98-99 -1.1989886965407734 1104 100-101 -1.3974632068957238 1105 1 0.06017770980484016 1105 2 0.0696133052414254 1105 3 0.15141439987810656 1105 4 0.028773208046146692 1105 5 0.035742498912071596 1105 6 -0.010009771424705605 1105 7 0.016629411756639456 1105 8 -0.01086597740358286 1105 9 0.12806077843786312 1105 10-11 0.10163645769232943 1105 12-13 0.04343368999366248 1105 14-15 -0.001976216061045477 1105 16-17 0.03950861805453343 1105 18-19 0.11984553422609423 1105 20-21 0.14200658007245437 1105 22-23 0.18839013709306585 1105 24-25 0.12200710615352506 1105 26-27 0.13215399352245072 1105 28-29 0.08772132292416046 1105 30-31 -0.10126114415409404 1105 32-33 -0.10021981918664125 1105 34-35 -0.10721523588766502 1105 36-37 0.10749306469185882 1105 38-39 -0.15315010962005715 1105 40-41 -0.1811236415434152 1105 42-43 -0.03189521851108168 1105 44-45 0.05572651659787198 1105 46-47 -0.045510549781788256 1105 48-49 -0.059824982538792426 1105 50-51 -0.08237360136625682 1105 52-53 -0.11653832024467903 1105 54-55 0.049426820425729545 1105 56-57 -0.061291923719899444 1105 58-59 0.006477037357335291 1105 60-61 0.05377147367961044 1105 62-63 -0.013171055387431352 1105 64-65 -0.2593360393895239 1105 66-67 -0.28556124771341374 1105 68-69 -0.20530765190932243 1105 70-71 -0.3420426489019128 1105 72-73 -0.24242025323547267 1105 74-75 -0.4373923303523384 1105 76-77 -0.46905539226749937 1105 78-79 -0.49060188053444875 1105 80-81 -0.3437944080256088 1105 82-83 -0.3343946487074412 1105 84-85 -0.673265200039701 1105 86-87 -0.8911861507322314 1105 88-89 -0.794002533077034 1105 90-91 -0.9397411678440548 1105 92-93 -1.0678885215282996 1105 94-95 -0.9307058539582655 1105 96-97 -1.0715126648093332 1105 98-99 -1.2552612325069568 1105 100-101 -1.3783004028124495 1106 1 0.26116693343944775 1106 2 0.2377483768058326 1106 3 0.3066887301086467 1106 4 0.20143777282110875 1106 5 0.2581080445698305 1106 6 0.22957502155563958 1106 7 0.3568551982978576 1106 8 0.2866430307037149 1106 9 0.5236547296606275 1106 10-11 0.4143201646952903 1106 12-13 0.47111818937643335 1106 14-15 0.3149306668001941 1106 16-17 0.01834603005867308 1106 18-19 0.2686448702952191 1106 20-21 0.4980468079442133 1106 22-23 0.6001008850996001 1106 24-25 0.43225779010241183 1106 26-27 0.3778132118189177 1106 28-29 0.3864916745739819 1106 30-31 0.3321084718336351 1106 32-33 0.4115705838427459 1106 34-35 0.2629185094831712 1106 36-37 0.2683115513298304 1106 38-39 0.27005369046668193 1106 40-41 0.206150321690167 1106 42-43 0.10507138476519629 1106 44-45 0.35149812771454236 1106 46-47 0.5068200894601986 1106 48-49 0.6270092099010682 1106 50-51 0.6637577084792738 1106 52-53 0.3455861334302952 1106 54-55 0.2740089443372469 1106 56-57 0.20522002036310738 1106 58-59 0.3119865792783969 1106 60-61 0.5594134264370894 1106 62-63 0.3462559706026269 1106 64-65 0.09244556405357685 1106 66-67 0.033416457221029816 1106 68-69 0.125790544801049 1106 70-71 -0.05242219883018251 1106 72-73 0.039364603135258136 1106 74-75 0.15533841898873746 1106 76-77 -0.34209529484648726 1106 78-79 -0.1191961570499771 1106 80-81 0.11509171448465239 1106 82-83 0.0268452588760546 1106 84-85 -0.08860504756914622 1106 86-87 0.059849156043867424 1106 88-89 -0.1508726745376805 1106 90-91 -0.2662564899673008 1106 92-93 0.11213162160051837 1106 94-95 0.05971865309808777 1106 96-97 0.010391048532675029 1106 98-99 -0.33946263877259497 1106 100-101 -0.7847313990423892 1107 1 0.11786370273399172 1107 2 0.12118960694564862 1107 3 0.12783417965285082 1107 4 0.11276829347647066 1107 5 0.10878583797359198 1107 6 0.05475391329853352 1107 7 0.10956772788715341 1107 8 0.23109380302788907 1107 9 0.28563553681397025 1107 10-11 0.2204327028078552 1107 12-13 0.1648086475794983 1107 14-15 0.17012676004473093 1107 16-17 0.2078651241129208 1107 18-19 0.19280678136655638 1107 20-21 0.16590866903820967 1107 22-23 0.3771663244158816 1107 24-25 0.3314916646631687 1107 26-27 0.2734721571809047 1107 28-29 0.44432281316617406 1107 30-31 0.3577797759321584 1107 32-33 0.3833422997700211 1107 34-35 0.30246278591594944 1107 36-37 0.2916532917033834 1107 38-39 0.2756809835159615 1107 40-41 0.3120333127368653 1107 42-43 0.23597345630639666 1107 44-45 0.21094113224349087 1107 46-47 0.22852904315174527 1107 48-49 0.22748476943414886 1107 50-51 0.16062908851235846 1107 52-53 0.11466023548479143 1107 54-55 0.07852840644164161 1107 56-57 -0.0824572732673019 1107 58-59 -0.09970133744153742 1107 60-61 0.20690061248062364 1107 62-63 0.15818796268373347 1107 64-65 0.1115607327561392 1107 66-67 -0.008349486130242667 1107 68-69 0.05641541646777526 1107 70-71 0.05310920747006165 1107 72-73 0.1600976428033185 1107 74-75 -0.09582597545254146 1107 76-77 -0.17029142295893251 1107 78-79 0.07058909537430935 1107 80-81 -0.030770045879833674 1107 82-83 0.04326068288919416 1107 84-85 -0.04543063441542117 1107 86-87 -0.19157519802812217 1107 88-89 -0.16003228045282825 1107 90-91 -0.526829408450002 1107 92-93 -0.9577953882438557 1107 94-95 -0.9970747777879083 1107 96-97 -1.2246907167133187 1107 98-99 -1.4708547981421844 1107 100-101 -0.763809878956538 1108 1 -0.10740601320994614 1108 2 -0.03149350707430898 1108 3 -0.0470808267491023 1108 4 0.041331660263821846 1108 5 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-0.622872652947283 2316 82-83 -0.7053148905784887 2316 84-85 -0.6303529115860798 2316 86-87 -0.6372071722076704 2316 88-89 -0.8755258183105532 2316 90-91 -0.34447160510042707 2316 92-93 -0.24160517043999974 2316 94-95 -0.17455905469428856 2316 96-97 -0.30480806708035146 2316 98-99 -0.27594874309940565 2316 100-101 -0.0467704594669307 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4701.0 3 1194.0 4 937.0 5 1095.0 6 1308.0 7 1641.0 8 2072.0 9 2680.0 10 3446.0 11 4772.0 12 6692.0 13 8845.0 14 10723.0 15 14026.0 16 17954.0 17 24650.0 18 23210.0 19 15836.0 20 15344.0 21 16175.0 22 17108.0 23 18923.0 24 21467.0 25 24466.0 26 27412.0 27 32545.0 28 37998.0 29 44615.0 30 53154.0 31 64844.0 32 80178.0 33 102155.0 34 135834.0 35 195204.0 36 230204.0 37 258012.0 38 143311.0 39 9361.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.62631604134763 18.695145003828483 14.501339969372129 21.17719898545176 2 15.717285960521062 20.778856750793093 40.06225212109042 23.441605167595426 3 19.12169236199604 26.323922680689915 29.959078916668165 24.595306040645884 4 12.311615198547232 17.092802917018574 36.69373458289171 33.90184730154249 5 12.919011382721104 38.50026754003225 33.02144270513497 15.559278372111676 6 30.598799420360294 36.33881269628831 17.297971560304617 15.764416323046785 7 27.841229343503667 34.95709240779146 19.905688262670335 17.295989986034538 8 27.350983757377506 33.567010457185795 19.331237472509013 19.750768312927683 9 28.02514170469083 16.424346711021716 20.11186850426714 35.43864308002031 10-11 25.329693657276465 27.19824673655004 25.762777236277962 21.709282369895533 12-13 26.806759214139202 25.23014969190229 27.02560894878838 20.937482145170126 14-15 23.32822279475674 25.664903401249862 26.946705437369783 24.060168366623618 16-17 21.19469802058079 29.51847551641899 27.136215696936087 22.150610766064123 18-19 21.110416585666393 26.61348063918938 31.417958434147526 20.858144340996706 20-21 22.34749470324409 25.434806535317488 31.61789424193882 20.5998045194996 22-23 21.826194509858844 25.724783085047896 31.68450138066034 20.764521024432913 24-25 21.392483834344194 26.080142381690568 31.820158706735562 20.707215077229677 26-27 21.06414761585133 26.366460036061646 31.784770355268243 20.784621992818778 28-29 20.765897686720194 26.67848332484516 31.917866350545644 20.637752637889008 30-31 20.811803639022852 26.236972685055214 31.81811522904665 21.133108446875283 32-33 20.687729896228955 26.45384132300607 31.709770029008045 21.14865875175693 34-35 21.448317241218497 26.48623488159837 31.346544841529767 20.718903035653373 36-37 21.524189539304807 26.148658969929393 31.28131513945093 21.045836351314872 38-39 20.989446198082284 26.36705308457475 31.47069203499785 21.172808682345114 40-41 21.088373366295325 26.427791837364122 31.46336186028052 21.02047293606003 42-43 21.15566404971657 26.409179658022236 31.0017582150904 21.43339807717079 44-45 20.974935780463454 26.634113429763985 30.92445953847988 21.466491251292684 46-47 21.399612050636026 26.933254599913397 30.34494979997388 21.32218354947669 48-49 21.385575559205623 27.062865830391846 30.031640435095575 21.519918175306955 50-51 21.249581999392163 27.31194305808199 29.84570659958336 21.59276834294249 52-53 21.59496845274604 27.367283888837267 29.552781562864016 21.48496609555267 54-55 21.157637271264367 27.322438990134128 29.611775094617027 21.90814864398448 56-57 21.457823953575637 27.529630665318678 29.347294820041057 21.665250561064624 58-59 21.65431239157947 27.024559631478525 29.453149630417663 21.867978346524342 60-61 21.43825649943379 27.165018838722016 29.891061093631883 21.505663568212306 62-63 21.223496814283532 27.291464144590076 29.85536748623796 21.62967155488843 64-65 21.11294669041466 27.331078648348324 29.71558878920189 21.840385872035124 66-67 21.263544248728937 27.396522298261505 29.3911990170933 21.948734435916254 68-69 21.22916047754792 27.25915038168806 29.601760469299084 21.90992867146494 70-71 21.286394943257477 27.49517084914163 29.342590002701456 21.875844204899437 72-73 21.416137909477406 27.47476443870954 29.158051607578244 21.95104604423481 74-75 21.186206698722433 27.932976919132702 28.943295241715045 21.937521140429826 76-77 21.34592807019116 27.54453416501615 29.08347598008024 22.02606178471245 78-79 21.463434744655338 27.69664911849883 28.916327443912483 21.92358869293335 80-81 21.516837836876984 28.04583340428704 28.73072337226974 21.70660538656624 82-83 21.67415912939256 27.8693921545431 28.61926578645167 21.837182929612673 84-85 21.492769165967132 27.803146854191375 28.554848885875007 22.14923509396649 86-87 21.71492178285616 27.61299392832774 28.563358216713645 22.108726072102453 88-89 21.66774159034111 27.668296286821114 28.399742496504754 22.26421962633302 90-91 21.676307664338992 27.679301327174578 28.50163168378883 22.1427593246976 92-93 22.045146251831625 27.36111202447305 28.483072546910766 22.11066917678456 94-95 21.77952894294625 27.268200271283145 28.58007963935722 22.37219114641339 96-97 21.999568420838695 27.03962996570081 28.512647899548455 22.44815371391203 98-99 21.966883116689065 27.291738948661926 27.998047975222555 22.743329959426454 100-101 22.80444031598283 26.20711146786549 28.132568278620916 22.855879937530766 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5806.0 1 3686.5 2 2274.5 3 2903.0 4 2233.5 5 2060.5 6 4013.5 7 8151.0 8 12319.5 9 12844.0 10 9145.0 11 5648.0 12 4822.0 13 4890.5 14 4875.0 15 4716.0 16 4554.0 17 4590.5 18 4709.0 19 4788.5 20 5068.0 21 5586.0 22 6210.0 23 6951.0 24 7820.0 25 9166.0 26 10895.5 27 12757.0 28 14444.5 29 16461.0 30 19049.5 31 21493.5 32 24422.0 33 27022.0 34 29180.5 35 31200.5 36 34234.5 37 37675.0 38 39814.5 39 40387.0 40 41050.0 41 43946.0 42 46926.0 43 49898.0 44 52633.5 45 55527.5 46 56522.0 47 58024.5 48 63729.0 49 64158.0 50 59141.5 51 55190.0 52 52915.5 53 52954.5 54 53082.5 55 50373.5 56 46254.5 57 42587.0 58 39321.0 59 34857.5 60 29949.0 61 25679.0 62 21274.5 63 16912.5 64 13108.0 65 10284.5 66 7869.5 67 6253.0 68 4705.0 69 3127.0 70 2289.5 71 1735.5 72 1409.0 73 1221.0 74 985.0 75 822.5 76 580.0 77 309.0 78 186.0 79 129.0 80 70.5 81 38.5 82 26.0 83 17.5 84 11.5 85 7.5 86 7.0 87 5.5 88 5.0 89 4.0 90 1.5 91 2.5 92 2.5 93 1.5 94 2.0 95 1.5 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1440781032344698 2 0.053521550786934054 3 0.1542328617543122 4 0.1342817479565042 5 0.19795805726328064 6 0.20303543652320186 7 0.16880792692396834 8 0.07592175340423346 9 0.1271734169926145 10-11 0.10644576283740678 12-13 0.15683128525791892 14-15 0.14234582089873196 16-17 0.13971753045830215 18-19 0.13661136902870333 20-21 0.1382241836171489 22-23 0.1991228677993802 24-25 0.21817797349249624 26-27 0.1499320228517907 28-29 0.12893556626517538 30-31 0.10109958114607799 32-33 0.12794995735001422 34-35 0.0947080566659419 36-37 0.0883165321858058 38-39 0.053969554839280044 40-41 0.06295950282302286 42-43 0.06502032146381442 44-45 0.043635594698499244 46-47 0.05588103879595625 48-49 0.04530814316059094 50-51 0.056657579153355966 52-53 0.029359198897073755 54-55 0.053730619344695515 56-57 0.029150130339312297 58-59 0.054925296817618145 60-61 0.0404398324584312 62-63 0.05498503069126428 64-65 0.04106703813171558 66-67 0.04109690506853864 68-69 0.06743954334648275 70-71 0.05495516375444121 72-73 0.05420849033386457 74-75 0.045278276223767865 76-77 0.02251967036459167 78-79 0.024670089815852416 80-81 0.02780611818227433 82-83 0.024490888194914018 84-85 0.021324992891669035 86-87 0.024968759184083075 88-89 0.06600593037897559 90-91 0.1130762228121274 92-93 0.08332875373635379 94-95 0.07771376961361741 96-97 0.06964969667138962 98-99 0.05719518401617116 100-101 0.13314680435722767 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1674092.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.82075178440903 #Duplication Level Percentage of deduplicated Percentage of total 1 73.47758718179135 34.40275871155927 2 13.836029505462804 12.95626606314067 3 5.128528547775786 7.203646864639973 4 2.443100637581245 4.575512341460916 5 1.3258699413714716 3.103911371168131 6 0.8007759788710168 2.2495760004982226 7 0.5288140614862472 1.7331630339066955 8 0.39120094676401246 1.465305794101091 9 0.2834640567939147 1.1944800218654552 >10 1.553582567705455 13.562055235266548 >50 0.14459797718001113 4.651397950239422 >100 0.07968103680493155 6.677197284693809 >500 0.004724523306426375 1.5307436725665258 >1k 0.0015322778291112567 1.475322668642562 >5k 1.2768981909260473E-4 0.3182971142299712 >10k+ 3.830694572778142E-4 2.9003658720207723 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21144 1.2630130243738098 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16142 0.9642241883958588 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11227 0.6706321994251212 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5324 0.3180231432920055 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3908 0.23343997820908288 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3311 0.19777885564234224 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2735 0.16337214442217035 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2652 0.1584142329095414 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2440 0.14575065169656148 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2156 0.12878623158106006 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1764 0.10537055311177641 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.9733873646131755E-5 0.0 10-11 0.0 0.0 0.0 5.9733873646131755E-5 0.0 12-13 0.0 0.0 0.0 5.9733873646131755E-5 0.0 14-15 0.0 0.0 0.0 1.1946774729226351E-4 0.0 16-17 0.0 0.0 0.0 2.6880243140759287E-4 0.0 18-19 0.0 0.0 0.0 3.285363050537246E-4 0.0 20-21 0.0 0.0 0.0 4.181371155229223E-4 0.0 22-23 0.0 0.0 0.0 6.272056732843834E-4 0.0 24-25 0.0 0.0 0.0 0.0013141452202148985 0.0 26-27 0.0 0.0 0.0 0.0033450969241833784 0.0 28-29 0.0 0.0 0.0 0.009587286720204147 0.0 30-31 0.0 0.0 0.0 0.027178912508989947 0.0 32-33 0.0 0.0 0.0 0.05973387364613175 0.0 34-35 0.0 0.0 0.0 0.10737163787892182 0.0 36-37 0.0 0.0 0.0 0.18263631867304783 0.0 38-39 0.0 0.0 0.0 0.29386079140214516 0.0 40-41 0.0 0.0 0.0 0.4185253857016221 0.0 42-43 0.0 0.0 0.0 0.5392176774036314 0.0 44-45 0.0 0.0 0.0 0.6756199778745733 0.0 46-47 0.0 0.0 0.0 0.8353184890675065 0.0 48-49 0.0 0.0 0.0 1.0010799884355222 0.0 50-51 0.0 0.0 0.0 1.1838955087295084 0.0 52-53 0.0 0.0 0.0 1.3564368027563598 0.0 54-55 0.0 0.0 0.0 1.5402379319655073 0.0 56-57 0.0 0.0 0.0 1.7444083120879856 0.0 58-59 0.0 0.0 0.0 1.9608241363079209 0.0 60-61 0.0 0.0 0.0 2.196504134778734 0.0 62-63 0.0 0.0 0.0 2.4264198144426947 0.0 64-65 0.0 0.0 0.0 2.653229332677057 0.0 66-67 0.0 0.0 0.0 2.908382573956509 0.0 68-69 0.0 0.0 0.0 3.1855178807377373 5.9733873646131755E-5 70-71 0.0 0.0 0.0 3.4871739426507027 5.9733873646131755E-5 72-73 0.0 0.0 0.0 3.780168592884979 5.9733873646131755E-5 74-75 0.0 0.0 0.0 4.064203162072335 5.9733873646131755E-5 76-77 0.0 0.0 0.0 4.3702795306351145 5.9733873646131755E-5 78-79 0.0 0.0 0.0 4.706581239262836 5.9733873646131755E-5 80-81 0.0 0.0 0.0 5.069852791841787 5.9733873646131755E-5 82-83 0.0 0.0 0.0 5.4373654494496115 5.9733873646131755E-5 84-85 0.0 0.0 0.0 5.794723348537595 5.9733873646131755E-5 86-87 0.0 0.0 0.0 6.155784747791639 5.9733873646131755E-5 88-89 0.0 0.0 0.0 6.540560494883197 5.9733873646131755E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9215 0.0 40.35937 1 GTATCAA 14800 0.0 35.875816 1 TAGGACG 70 2.0374768E-4 33.97449 4 GTACATG 11930 0.0 28.859346 1 TATCAAC 18645 0.0 28.29027 2 ATCAACG 18675 0.0 28.271141 3 TACATGG 11985 0.0 28.136894 2 TCAACGC 18880 0.0 27.913788 4 CAACGCA 18975 0.0 27.748968 5 ATACCGT 260 0.0 27.43518 6 ACATGGG 11945 0.0 27.196657 3 AACGCAG 19450 0.0 27.065617 6 CATGGGA 7840 0.0 24.692177 4 GAGTACT 11995 0.0 24.532978 12-13 ACGCAGA 21510 0.0 24.293058 7 CGCAGAG 21675 0.0 24.084028 8 CATGGGG 3990 0.0 23.841747 4 GTAAGCG 150 0.0 23.751547 94-95 TACCGTC 265 3.092282E-11 23.325039 7 ATGGGAG 2800 0.0 23.272526 5 >>END_MODULE