FastQCFastQC Report
Thu 26 May 2016
SRR938020_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938020_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1674092
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203071.2130157721319974No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164230.9810094068904217No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116050.693211603663359No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51090.3051803604580871No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41490.24783584175780063No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35060.20942696100333794No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27380.16355134604310875No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23850.14246528864602423No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22310.13326627210451994No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20530.12263364259550849No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17480.10441481113343831No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96850.036.8376771
GTATCAA150950.034.9649471
CGTAAGG856.403379E-427.944932
TATCAAC189350.027.7486442
ATCAACG189900.027.6624893
TCAACGC191000.027.5239334
CAACGCA191850.027.3277285
CTATCGT700.00840182627.1367744
GTACATG121350.026.9339831
AACGCAG195900.026.7385166
TACATGG122050.026.0788822
ACATGGG121000.025.396863
GAGTACT117750.023.9758612-13
ACGCAGA218100.023.9072697
CATGGGG38900.023.8057164
GGGTACG1000.001652667323.7531911
CGCAGAG219800.023.7007568
AGTACTT119550.022.93959212-13
GCAGAGT226000.022.7984129
ATGGGAG28250.022.6940275
Produced by FastQC (version 0.11.5)