##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938020_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1674092 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.55113219584109 34.0 31.0 34.0 31.0 34.0 2 32.74996953572444 34.0 31.0 34.0 31.0 34.0 3 32.78638569445407 34.0 31.0 34.0 31.0 34.0 4 36.245249365028926 37.0 35.0 37.0 35.0 37.0 5 36.232060125727855 37.0 37.0 37.0 35.0 37.0 6 36.28199107336992 37.0 37.0 37.0 35.0 37.0 7 36.23719186281279 37.0 37.0 37.0 35.0 37.0 8 36.23422906267995 37.0 37.0 37.0 35.0 37.0 9 37.90912984471582 39.0 38.0 39.0 35.0 39.0 10-11 37.90217801650088 39.0 38.0 39.0 35.0 39.0 12-13 37.81869634404799 39.0 38.0 39.0 35.0 39.0 14-15 39.05813181115494 40.0 38.5 41.0 36.0 41.0 16-17 38.96490425854732 40.0 38.0 41.0 36.0 41.0 18-19 39.249438202918356 40.5 39.0 41.0 36.0 41.0 20-21 39.25411566389422 41.0 39.0 41.0 36.0 41.0 22-23 39.14838790221803 40.5 39.0 41.0 35.5 41.0 24-25 39.04037143717311 40.0 38.5 41.0 35.0 41.0 26-27 38.8617725310198 40.0 38.0 41.0 35.0 41.0 28-29 38.729200366527046 40.0 38.0 41.0 35.0 41.0 30-31 38.439707913304645 40.0 38.0 41.0 34.5 41.0 32-33 38.161394953204486 40.0 37.0 41.0 34.0 41.0 34-35 37.965631817128326 40.0 37.0 41.0 34.0 41.0 36-37 37.925909388492386 40.0 37.0 41.0 33.5 41.0 38-39 37.77292765272159 40.0 37.0 41.0 33.0 41.0 40-41 37.58070135930403 40.0 36.5 41.0 33.0 41.0 42-43 37.31203034241845 40.0 36.0 41.0 33.0 41.0 44-45 36.966077431825724 40.0 36.0 41.0 32.0 41.0 46-47 36.45683809491952 39.5 35.0 41.0 30.5 41.0 48-49 35.970890787364134 39.0 35.0 41.0 29.5 41.0 50-51 35.58074496503179 39.0 35.0 40.5 28.0 41.0 52-53 35.100004659242146 38.0 35.0 40.0 27.0 41.0 54-55 34.811885189105496 38.0 34.5 40.0 26.0 41.0 56-57 34.48410123219034 38.0 34.0 40.0 25.0 41.0 58-59 34.07580138964883 37.5 34.0 40.0 23.5 41.0 60-61 33.633916475319154 37.0 33.0 40.0 22.5 41.0 62-63 33.21304802842377 36.0 33.0 39.0 22.0 41.0 64-65 32.76188345682316 36.0 32.5 39.0 20.0 40.0 66-67 32.24169131684519 35.0 31.5 38.5 18.5 40.0 68-69 31.70855245709316 35.0 31.0 38.0 14.5 40.0 70-71 31.16195406226181 35.0 30.5 37.5 9.5 39.5 72-73 30.63109076442633 34.5 30.0 37.0 7.5 39.0 74-75 30.07728846443326 34.0 29.0 36.0 4.5 39.0 76-77 28.69948903644483 32.5 28.0 35.0 2.0 37.0 78-79 29.418314226458286 34.0 29.0 35.0 2.0 37.0 80-81 29.1182715764725 34.0 29.0 35.0 2.0 37.0 82-83 28.602867703806005 34.0 28.5 35.0 2.0 36.5 84-85 28.063634196925854 33.5 27.0 35.0 2.0 36.0 86-87 27.468677348676177 33.0 26.0 35.0 2.0 35.5 88-89 26.780395282935466 33.0 25.0 35.0 2.0 35.0 90-91 26.28713804259264 32.0 24.5 35.0 2.0 35.0 92-93 25.51887351471723 32.0 21.5 34.0 2.0 35.0 94-95 24.756814738974917 31.5 18.5 34.0 2.0 35.0 96-97 23.773788716510204 31.0 4.5 34.0 2.0 35.0 98-99 22.398630123075673 31.0 2.0 34.0 2.0 35.0 100-101 18.017578185667215 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.07511304539630004 1101 2 0.14557220893028955 1101 3 0.08251736752674077 1101 4 0.0271591345848492 1101 5 0.031589958642392446 1101 6 0.029040147752979806 1101 7 0.020256830211003773 1101 8 0.033020699116569574 1101 9 0.07296101506097585 1101 10-11 0.07313071528454174 1101 12-13 0.13405606211198773 1101 14-15 0.18840657543757544 1101 16-17 0.09500872983082331 1101 18-19 0.175987359992277 1101 20-21 0.1248991193359501 1101 22-23 0.11785991266304308 1101 24-25 0.17204792334830188 1101 26-27 0.13800144142805237 1101 28-29 0.10362748916558928 1101 30-31 0.1560330477429872 1101 32-33 0.004977991056513531 1101 34-35 0.17362909913358493 1101 36-37 0.35484868777181333 1101 38-39 0.3188346549870076 1101 40-41 0.2734736618366824 1101 42-43 0.34772372033057763 1101 44-45 0.2865167515889695 1101 46-47 0.16899767796672194 1101 48-49 0.12556830530210306 1101 50-51 -0.15371351000489852 1101 52-53 -0.3103377710459725 1101 54-55 -0.30733776813077185 1101 56-57 -0.09887341564048313 1101 58-59 -0.02214470689095549 1101 60-61 -0.2024822229517298 1101 62-63 -0.3592729140616626 1101 64-65 -0.4143625165168672 1101 66-67 -0.6204733574181418 1101 68-69 -0.4965122014350669 1101 70-71 -0.36805396953512215 1101 72-73 -0.4416738365592785 1101 74-75 -0.5687846112506598 1101 76-77 -0.6704795481329455 1101 78-79 -0.7967454028525509 1101 80-81 -0.735431041284464 1101 82-83 -0.8200070156950439 1101 84-85 -0.9468147041287018 1101 86-87 -1.0728344431671104 1101 88-89 -1.0514739594420455 1101 90-91 -1.351286066011312 1101 92-93 -1.2867717765209186 1101 94-95 -1.340268266319967 1101 96-97 -1.344225936698784 1101 98-99 -1.3144155621441733 1101 100-101 -1.690083286398572 1102 1 0.1763130691591428 1102 2 0.17299973224758247 1102 3 0.16921091591383686 1102 4 0.12701878527099808 1102 5 0.10430426981687191 1102 6 0.06690847187820737 1102 7 0.09517291765827451 1102 8 0.11822535663453948 1102 9 0.21836364679625575 1102 10-11 0.15910395237835218 1102 12-13 0.1447010748845159 1102 14-15 0.19203040957290085 1102 16-17 0.26734320675115697 1102 18-19 0.1899517454256454 1102 20-21 0.06289665394059796 1102 22-23 0.12743188438525976 1102 24-25 0.2634049304177495 1102 26-27 0.17994100953831094 1102 28-29 0.15639771728892526 1102 30-31 0.1396886646858988 1102 32-33 0.011913771963072861 1102 34-35 0.1368133948622159 1102 36-37 0.22533931629910597 1102 38-39 0.40144582055464895 1102 40-41 0.31732354005208663 1102 42-43 0.2720056368041739 1102 44-45 0.35119439372050465 1102 46-47 0.23527002989448675 1102 48-49 0.3009622487465222 1102 50-51 0.2743147677827764 1102 52-53 0.42975980514366086 1102 54-55 0.609941534429673 1102 56-57 0.703170931772334 1102 58-59 0.5811030059350415 1102 60-61 0.5523223276334264 1102 62-63 0.665353192395898 1102 64-65 0.5718438935110512 1102 66-67 0.567622570223989 1102 68-69 0.8420025463817211 1102 70-71 0.6418354589684085 1102 72-73 0.4920630938749859 1102 74-75 0.5376877941435048 1102 76-77 0.49943223229835 1102 78-79 0.3981563526277583 1102 80-81 0.12543934723808192 1102 82-83 0.13285529345959546 1102 84-85 -0.003396270100306964 1102 86-87 -0.23719908405108825 1102 88-89 -0.17324555392906404 1102 90-91 -0.24180246237559544 1102 92-93 -0.33092797268322016 1102 94-95 -0.5810434549375643 1102 96-97 -0.26241676721028284 1102 98-99 -0.4547945795504589 1102 100-101 -0.7277566809212388 1103 1 0.11217875343049855 1103 2 0.12507313327538583 1103 3 0.08275729075131011 1103 4 0.08166942609819472 1103 5 0.12240686408348012 1103 6 0.06270981544826526 1103 7 0.08023189233476558 1103 8 0.06421425309089557 1103 9 0.13120315105812352 1103 10-11 0.1119861268567135 1103 12-13 0.17618777333456137 1103 14-15 0.1720935635571763 1103 16-17 0.1447108362065066 1103 18-19 0.1696227668278283 1103 20-21 0.16532839191348359 1103 22-23 0.19184353072294158 1103 24-25 0.12275187487939831 1103 26-27 0.0950023834470457 1103 28-29 0.1354979536003995 1103 30-31 0.12413364229306012 1103 32-33 0.17127438946869233 1103 34-35 0.1355628273019036 1103 36-37 0.21658663328670258 1103 38-39 0.24959957264429988 1103 40-41 0.2137102446937007 1103 42-43 0.18290640662235091 1103 44-45 0.13812644643137872 1103 46-47 0.12486019974981133 1103 48-49 -0.017691265999722816 1103 50-51 0.011980650184284514 1103 52-53 -0.04265547358005506 1103 54-55 0.06753961917159046 1103 56-57 0.07064559794367398 1103 58-59 0.16044084363343103 1103 60-61 -0.11651525724263223 1103 62-63 0.01623123400322868 1103 64-65 0.04972323018782987 1103 66-67 0.009116775949117084 1103 68-69 0.11443859265336442 1103 70-71 0.02213763324038709 1103 72-73 -0.22420640956132942 1103 74-75 -0.15540396488070485 1103 76-77 -0.0876212009256001 1103 78-79 -0.18319632419307652 1103 80-81 -0.10511848715813699 1103 82-83 -0.17131382403571038 1103 84-85 -0.26166975161847716 1103 86-87 -0.48955832909267727 1103 88-89 -0.6014188610514317 1103 90-91 -0.6091661963033204 1103 92-93 -0.49628086620890244 1103 94-95 -0.3605649757928262 1103 96-97 -1.0103058298070238 1103 98-99 -1.01569767120629 1103 100-101 -1.1174932555399408 1104 1 0.09471001469415086 1104 2 0.09783968988442382 1104 3 0.08267420566977535 1104 4 0.05059560560531651 1104 5 -0.05587806717264954 1104 6 -0.02627952477590867 1104 7 0.0162318955457863 1104 8 0.10269669404229376 1104 9 0.1164433070814681 1104 10-11 0.0759932280222344 1104 12-13 0.03141502909452498 1104 14-15 0.0502521011312993 1104 16-17 0.04846419473027197 1104 18-19 0.09714066624430728 1104 20-21 0.09728130876177232 1104 22-23 0.1516819282324775 1104 24-25 0.16227890000801892 1104 26-27 0.041597540750792916 1104 28-29 -0.11313495563777565 1104 30-31 -0.1658581776394925 1104 32-33 -0.03832839962122847 1104 34-35 -0.113857383324806 1104 36-37 -0.08841749688473755 1104 38-39 0.025000127026494567 1104 40-41 0.09289490309360815 1104 42-43 0.08664150248326763 1104 44-45 -0.022939202021596827 1104 46-47 0.08890884345608896 1104 48-49 0.25461446943972277 1104 50-51 0.13797857426137483 1104 52-53 0.054165337988600015 1104 54-55 -0.014550849645011965 1104 56-57 0.03458847875631932 1104 58-59 0.09133332190486954 1104 60-61 -0.09183637907587183 1104 62-63 -0.20988501607403975 1104 64-65 -0.09169359923729559 1104 66-67 -0.33876909003423705 1104 68-69 -0.15903334425655657 1104 70-71 -0.14428579883976766 1104 72-73 -0.5778331737144917 1104 74-75 -0.46080284953372797 1104 76-77 -0.45073371257557326 1104 78-79 -0.2818925707542803 1104 80-81 -0.21271507256197353 1104 82-83 -0.37548959193051346 1104 84-85 -0.49893565839781573 1104 86-87 -0.39254726472863055 1104 88-89 -0.6725629707205272 1104 90-91 -0.8818993984665795 1104 92-93 -0.7310604418889746 1104 94-95 -0.7353894736097324 1104 96-97 -1.1039204402584062 1104 98-99 -1.2868757373869713 1104 100-101 -1.4214269127044012 1105 1 0.06111315267516915 1105 2 0.06854597924623107 1105 3 0.014556202478196667 1105 4 0.00589445078932016 1105 5 0.011562334333000024 1105 6 -0.01784071906030249 1105 7 0.0028877104341376025 1105 8 0.03540094907634028 1105 9 0.043710760273654614 1105 10-11 0.058046300222592606 1105 12-13 0.1496500518132109 1105 14-15 0.013625826631049165 1105 16-17 0.026846416898351322 1105 18-19 0.09188240102643874 1105 20-21 0.1650700279879942 1105 22-23 0.2047625034477818 1105 24-25 0.1730771300210563 1105 26-27 0.18686696736271813 1105 28-29 0.07881589768354047 1105 30-31 0.11713607568913176 1105 32-33 0.22318321553755283 1105 34-35 0.25438829829681 1105 36-37 0.25348356413291384 1105 38-39 0.23972486847195995 1105 40-41 0.3148833062072214 1105 42-43 0.1806469354783644 1105 44-45 0.21867795150315317 1105 46-47 0.20635586870855605 1105 48-49 0.25113319560945513 1105 50-51 -0.0104911879537255 1105 52-53 -0.021608422228716506 1105 54-55 -0.0017807225908867963 1105 56-57 0.012601065397980449 1105 58-59 0.03711681217786378 1105 60-61 -0.08734517419348009 1105 62-63 -0.1607181616076474 1105 64-65 -0.051877267361689405 1105 66-67 -0.06559853845123342 1105 68-69 -0.047452903038884386 1105 70-71 -0.15471820007741854 1105 72-73 -0.12067701469584691 1105 74-75 -0.22585093641292175 1105 76-77 -0.17979772385573511 1105 78-79 -0.05125446791718602 1105 80-81 -0.3547419086341428 1105 82-83 -0.18269173650177706 1105 84-85 -0.3612363369131266 1105 86-87 -0.6464830646336104 1105 88-89 -1.012323876300922 1105 90-91 -0.9980955253257981 1105 92-93 -0.9035934388070324 1105 94-95 -0.8289620790110597 1105 96-97 -1.0028058996875764 1105 98-99 -1.3871824853861412 1105 100-101 -1.7676257956513766 1106 1 0.18408382628141595 1106 2 0.1755839801136716 1106 3 0.09961358745800908 1106 4 0.13162886175919652 1106 5 0.12376164704777892 1106 6 0.08318854060673431 1106 7 0.16606999115345644 1106 8 0.14507382132803315 1106 9 0.15960214585401644 1106 10-11 0.14500352005377692 1106 12-13 0.09504194618481421 1106 14-15 0.1685740300140921 1106 16-17 0.15924474205766614 1106 18-19 0.27076318539904776 1106 20-21 0.3401857571442406 1106 22-23 0.32612798237882146 1106 24-25 0.27680006399496193 1106 26-27 0.26542141636682715 1106 28-29 0.20125901315412875 1106 30-31 0.20871787430788658 1106 32-33 0.25663165246412234 1106 34-35 0.3222979586578063 1106 36-37 0.21076845386701137 1106 38-39 0.272296474584131 1106 40-41 0.16620115894134813 1106 42-43 0.13827046332008308 1106 44-45 0.07456971242131516 1106 46-47 0.05494772638046186 1106 48-49 0.07582437600515135 1106 50-51 0.095656636755173 1106 52-53 0.02627488107759035 1106 54-55 -0.07316455137012667 1106 56-57 -0.09027214549182361 1106 58-59 0.17979563978938273 1106 60-61 -0.10721514049648562 1106 62-63 -0.02269206311900973 1106 64-65 0.03772212208881598 1106 66-67 0.1319357006973263 1106 68-69 -0.18056770636187736 1106 70-71 -0.5446259920902321 1106 72-73 -0.4390483405557859 1106 74-75 -0.23438570103441592 1106 76-77 -0.15757918550455585 1106 78-79 -0.08390009898514705 1106 80-81 0.08679964543118501 1106 82-83 -0.030359012049775203 1106 84-85 -0.14121081394245039 1106 86-87 -0.2579465067314999 1106 88-89 -0.44366249772687283 1106 90-91 -0.4789685193537565 1106 92-93 -0.6065729954079728 1106 94-95 -0.44036129664025836 1106 96-97 -0.455435141004763 1106 98-99 -0.6625029821665791 1106 100-101 -1.4017569303392605 1107 1 0.011343557333610477 1107 2 0.03287156675074243 1107 3 0.07110248590328894 1107 4 0.08353904202628115 1107 5 0.038337982585538555 1107 6 0.02164823136712357 1107 7 -0.026404739298243385 1107 8 0.0057342970037908 1107 9 -0.03361914526590226 1107 10-11 -0.005792824239705396 1107 12-13 0.09990679429633076 1107 14-15 0.08302154800012573 1107 16-17 -0.015188751280625468 1107 18-19 0.11035821401010537 1107 20-21 0.049114847440492326 1107 22-23 0.08502801608446475 1107 24-25 0.11125575656848241 1107 26-27 0.0853856186471873 1107 28-29 0.030955623605976257 1107 30-31 0.004236766903105149 1107 32-33 0.042695917894143065 1107 34-35 0.10498681681127664 1107 36-37 0.0724298322341852 1107 38-39 0.04898805243448834 1107 40-41 -0.062052694931118424 1107 42-43 0.015467044715343548 1107 44-45 -0.12931825311370204 1107 46-47 -0.11448706481888138 1107 48-49 -0.1438018012710458 1107 50-51 -0.011924034025902586 1107 52-53 -0.16534829755845237 1107 54-55 0.0038542036905653276 1107 56-57 -0.024196296167680487 1107 58-59 0.039005610461593676 1107 60-61 -0.08309623317926906 1107 62-63 -0.08093541787673786 1107 64-65 -0.07475012886714438 1107 66-67 -0.11961262816128482 1107 68-69 -0.12354937746451355 1107 70-71 -0.22478673314951436 1107 72-73 -0.3955833614959019 1107 74-75 -0.09242488481541145 1107 76-77 -0.35565195870430344 1107 78-79 -0.18767687463339655 1107 80-81 0.06592035425702747 1107 82-83 -0.08174501142215362 1107 84-85 -0.13773971362747872 1107 86-87 -0.36339694199923045 1107 88-89 -0.44008640626447004 1107 90-91 -0.5818077356781366 1107 92-93 -0.4959690832286121 1107 94-95 -0.8821531972124781 1107 96-97 -0.9766435464952963 1107 98-99 -1.0183780310785053 1107 100-101 -1.7067252199133698 1108 1 -0.1403350583899865 1108 2 -0.07169344535716249 1108 3 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82-83 0.4413968296991193 2315 84-85 0.5470103879993609 2315 86-87 0.6328851856118334 2315 88-89 0.7715593957123303 2315 90-91 0.8168691577766367 2315 92-93 1.050380239009641 2315 94-95 1.1066868519369102 2315 96-97 1.0252626044329354 2315 98-99 1.4136765123031978 2315 100-101 2.0979710338854005 2316 1 -0.37221614543877024 2316 2 -0.41947305084843833 2316 3 -0.375026273371013 2316 4 -0.25856061300749644 2316 5 -0.2504077404799645 2316 6 -0.42142278331713356 2316 7 -0.3585754399423422 2316 8 -0.2109570636588174 2316 9 -0.3265398873790559 2316 10-11 -0.3515763361476516 2316 12-13 -0.47409534803667697 2316 14-15 -0.48950499921694757 2316 16-17 -0.3115739157651305 2316 18-19 -0.5964639358013955 2316 20-21 -0.5803145385083823 2316 22-23 -0.530645033534995 2316 24-25 -0.5153192939675222 2316 26-27 -0.4197393929500848 2316 28-29 -0.3523038151702522 2316 30-31 -0.46921615659423566 2316 32-33 -0.471798989144915 2316 34-35 -0.511875199512069 2316 36-37 -0.48356701330698115 2316 38-39 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quality scores pass #Quality Count 2 2.0 3 7.0 4 17.0 5 48.0 6 82.0 7 197.0 8 469.0 9 833.0 10 1430.0 11 2422.0 12 3829.0 13 6050.0 14 7553.0 15 10768.0 16 14836.0 17 22503.0 18 22322.0 19 14518.0 20 12221.0 21 12727.0 22 13263.0 23 14384.0 24 16209.0 25 18419.0 26 21582.0 27 25171.0 28 30360.0 29 37033.0 30 45204.0 31 55424.0 32 70260.0 33 91808.0 34 129345.0 35 197633.0 36 259371.0 37 324900.0 38 182223.0 39 8669.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.86624757833413 19.00993517731403 14.84995677890213 21.273860465449708 2 15.916594078996892 20.988889008374763 39.40494838807579 23.689568524552556 3 19.202781018675335 26.10514606740231 30.181328965141084 24.510743948781275 4 12.50411417792005 17.276799501101795 36.374111531704706 33.84497478927345 5 12.963815838597434 38.58214237713364 33.000612278422416 15.453429505846511 6 30.808196356046764 36.37460044765394 17.102124713496103 15.715078482803197 7 27.928692090996194 34.85555154674893 19.704412899649483 17.5113434626054 8 27.396881413924685 33.72926935915111 19.105819751841597 19.768029475082614 9 27.67052228909761 16.37866975052745 20.056424617046137 35.8943833433288 10-11 25.4223782205518 27.23843731407832 25.579508175177946 21.759676290191937 12-13 26.746246528324303 25.081903023355768 27.053130862349455 21.11871958597047 14-15 23.24881189325318 25.704829842087534 26.79365889090922 24.25269937375007 16-17 21.19709946847085 29.530213770535674 27.078327971049642 22.194358789943834 18-19 21.108517333575456 26.574704377059327 31.317753146183126 20.999025143182095 20-21 22.280914071628082 25.410491179696216 31.58309101291924 20.725503735756458 22-23 21.797846235451814 25.725109492190395 31.590109743072663 20.886934529285128 24-25 21.408873566006058 26.03973801096091 31.78688435965818 20.764504063374847 26-27 21.044045628266918 26.36694513698577 31.762733251005056 20.826275983742256 28-29 20.71819021132501 26.650589267350284 31.912821228140864 20.718399293183843 30-31 20.817563063159934 26.22279329715729 31.830199589495088 21.12944405018769 32-33 20.604751710180803 26.434240173180445 31.724779761207866 21.23622835543088 34-35 21.356630443775963 26.393455207365367 31.395601339712293 20.854313009146377 36-37 21.441629793124868 26.077645748180718 31.348100670369206 21.132623788325212 38-39 20.993023501701565 26.37488508400889 31.396333082245505 21.235758332044043 40-41 20.999548999286162 26.421832018661274 31.503982844052313 21.074636138000244 42-43 21.120821485747054 26.36944159040405 31.003112231487894 21.506624692360994 44-45 20.938245926747157 26.561562924857174 30.941041472033792 21.559149676361873 46-47 21.38524934613672 26.90983780128709 30.339256257957686 21.365656594618503 48-49 21.36879033549093 26.957966266925677 30.10954050095455 21.563702896628843 50-51 21.216790614918533 27.243964149739085 29.91478290114476 21.624462334197624 52-53 21.574074848471454 27.385327568099733 29.456546284160385 21.584051299268435 54-55 21.118073273758355 27.28126984430417 29.54991998059701 22.050736901340468 56-57 21.388990786223903 27.49650794181855 29.33499143863238 21.779509833325168 58-59 21.506492295999944 27.055027376863862 29.435207292211874 22.003273034924323 60-61 21.308462633018284 27.30929416529571 29.777547194833033 21.604696006852972 62-63 21.14166989049923 27.386933097134698 29.674903247681456 21.79649376468462 64-65 21.043788998096755 27.388101769740985 29.518058432132744 22.050050800029517 66-67 21.09742553602057 27.493618055957985 29.274588183142825 22.13436822487862 68-69 21.06820351257725 27.387914666810076 29.384947932771954 22.158933887840725 70-71 21.12635876424408 27.48631191303431 29.250789889769415 22.136539432952198 72-73 21.242130019441415 27.407559183717357 29.061289298379606 22.289021498461622 74-75 21.01483887830925 27.901213546917592 28.750143758634856 22.333803816138307 76-77 20.953827678299366 27.49643012911282 29.15066946281658 22.39907272977123 78-79 21.03452726223296 27.591157923416947 29.02118981808448 22.35312499626561 80-81 21.055488147660924 27.945431268142627 28.858126195942504 22.14095438825394 82-83 21.144075633126867 27.7289297068807 28.802966478328795 22.324028181663632 84-85 20.916489266678337 27.63412843181597 28.68959612550061 22.759786176005086 86-87 21.07216289098515 27.49021398084683 28.749026775839067 22.688596352328954 88-89 20.931903410610065 27.607757287954527 28.442463076644103 23.01787622479131 90-91 20.88191341954612 27.5407188408776 28.730690704112245 22.846677035464037 92-93 21.162313884856644 27.24944679673877 28.710375425377233 22.877863893027353 94-95 20.78092267453201 27.045408448415486 28.863950896157668 23.309717980894835 96-97 20.83216460477558 26.975629209103086 28.758907354551173 23.43329883157016 98-99 20.738078076876086 27.174269214765623 28.389454218184284 23.698198490174004 100-101 21.032359198966972 26.485777192190636 28.561422080651123 23.920441528191272 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5494.0 1 3485.0 2 2115.0 3 2760.5 4 2169.0 5 1954.5 6 4058.0 7 8288.0 8 12147.0 9 12686.0 10 9202.0 11 5708.0 12 4869.0 13 4880.5 14 4817.0 15 4579.5 16 4418.0 17 4495.5 18 4648.5 19 4739.5 20 4955.0 21 5332.0 22 5855.5 23 6634.5 24 7613.0 25 8856.0 26 10439.0 27 12331.5 28 14056.5 29 16059.5 30 18724.0 31 21151.5 32 24252.5 33 27083.5 34 29248.5 35 31230.5 36 34111.5 37 37731.5 38 39608.0 39 39995.5 40 40961.0 41 44046.0 42 47117.0 43 50094.5 44 52789.0 45 56004.5 46 57128.0 47 58657.5 48 64688.5 49 64726.0 50 59580.0 51 55726.5 52 53356.5 53 53965.0 54 54313.5 55 51276.5 56 46879.0 57 42899.0 58 39541.0 59 35429.5 60 30347.0 61 25398.0 62 20994.0 63 16464.0 64 12607.0 65 9925.5 66 7383.5 67 5907.0 68 4311.0 69 2654.5 70 1978.5 71 1542.5 72 1355.0 73 1188.5 74 940.0 75 770.5 76 514.0 77 259.0 78 139.5 79 95.5 80 46.5 81 18.0 82 13.5 83 8.0 84 4.5 85 3.0 86 1.5 87 2.5 88 2.0 89 0.5 90 0.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008422476184104577 2 0.015172403906117465 3 0.010333960140780793 4 1.7920162093839527E-4 5 0.00101547585198424 6 0.0018517500830300844 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 5.0773792599212E-4 14-15 0.0 16-17 0.0032256291768911144 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0031360283664219175 26-27 0.018218831462070186 28-29 0.0063616575433130314 30-31 0.0033450969241833784 32-33 0.0 34-35 0.008989947983742828 36-37 8.661411678689105E-4 38-39 0.002568556566783665 40-41 0.002210153324906875 42-43 0.0031061614295988514 44-45 0.0 46-47 2.0906855776146115E-4 48-49 4.7787098916905404E-4 50-51 0.004390439712990684 52-53 0.008960081046919763 54-55 0.007347266458474206 56-57 0.017561758851962737 58-59 0.02484929143679081 60-61 0.00442030664981375 62-63 0.021474327575784366 64-65 0.02225086793318408 66-67 0.013679057064964171 68-69 0.03192775546385743 70-71 0.01687481930503222 72-73 0.00528644781768266 74-75 0.016695617684093826 76-77 0.021623662259899694 78-79 0.027358114129928342 80-81 0.012125976350164746 82-83 0.03539232013533306 84-85 0.022639138111883932 86-87 0.03171868690609596 88-89 0.023206609911522187 90-91 0.028731993223789373 92-93 0.02571543260465972 94-95 0.015023069222002135 96-97 0.01579960957940185 98-99 0.011379302929588098 100-101 0.010662496445834517 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1674092.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.028438621668634 #Duplication Level Percentage of deduplicated Percentage of total 1 71.76806888612137 32.31604084834403 2 14.562242881575823 13.11430119573736 3 5.551675125505795 7.499497879088465 4 2.589217473934777 4.66353680412896 5 1.4514508591393904 3.2678282961563125 6 0.8582121112555402 2.318637082562565 7 0.5796626240143694 1.8270912020694492 8 0.4099098980710363 1.4766082148564883 9 0.294027613077216 1.191564389567084 >10 1.6845993158954877 14.104076725681644 >50 0.15809774882194616 4.930359839206031 >100 0.08514354573699676 6.898893905757294 >500 0.0054373894975384505 1.648231812808509 >1k 0.0017240503284878012 1.5518495749956425 >5k 1.3261925603752319E-4 0.3050909869801338 >10k+ 3.978577681125695E-4 2.8863912420600446 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20307 1.2130157721319974 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16423 0.9810094068904217 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11605 0.693211603663359 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5109 0.3051803604580871 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4149 0.24783584175780063 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3506 0.20942696100333794 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2738 0.16355134604310875 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2385 0.14246528864602423 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2231 0.13326627210451994 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2053 0.12263364259550849 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1748 0.10441481113343831 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.9733873646131755E-5 0.0 10-11 0.0 0.0 0.0 5.9733873646131755E-5 0.0 12-13 0.0 0.0 0.0 5.9733873646131755E-5 0.0 14-15 0.0 0.0 0.0 1.1946774729226351E-4 0.0 16-17 0.0 0.0 0.0 3.285363050537246E-4 0.0 18-19 0.0 0.0 0.0 3.882701786998564E-4 0.0 20-21 0.0 0.0 0.0 4.7787098916905404E-4 0.0 22-23 0.0 0.0 0.0 6.869395469305151E-4 0.0 24-25 0.0 0.0 0.0 0.0012842782833918328 0.0 26-27 0.0 0.0 0.0 0.0032554961137141805 0.0 28-29 0.0 0.0 0.0 0.009437952036088817 0.0 30-31 0.0 0.0 0.0 0.026939977014405418 0.0 32-33 0.0 0.0 0.0 0.059793607519777886 0.0 34-35 0.0 0.0 0.0 0.10784950886809089 0.0 36-37 0.0 0.0 0.0 0.18341285903044754 0.0 38-39 0.0 0.0 0.0 0.29496586806459857 0.0 40-41 0.0 0.0 0.0 0.4201382002900677 0.0 42-43 0.0 0.0 0.0 0.5413083629812458 0.0 44-45 0.0 0.0 0.0 0.6782482683150031 0.0 46-47 0.0 0.0 0.0 0.8380363803184054 0.0 48-49 0.0 0.0 0.0 1.004693887791113 0.0 50-51 0.0 0.0 0.0 1.1885248839370837 0.0 52-53 0.0 0.0 0.0 1.3612453795848736 0.0 54-55 0.0 0.0 0.0 1.546002250772359 0.0 56-57 0.0 0.0 0.0 1.7511582398099985 0.0 58-59 0.0 0.0 0.0 1.9696050157339022 0.0 60-61 0.0 0.0 0.0 2.207017296540453 0.0 62-63 0.0 0.0 0.0 2.437948452056398 0.0 64-65 0.0 0.0 0.0 2.6655345106481603 0.0 66-67 0.0 0.0 0.0 2.9214642922850116 0.0 68-69 0.0 0.0 0.0 3.2004214822124473 0.0 70-71 0.0 0.0 0.0 3.502465814304112 0.0 72-73 0.0 0.0 0.0 3.7963266057062572 0.0 74-75 0.0 0.0 0.0 4.082750529839459 0.0 76-77 0.0 0.0 0.0 4.391933059831837 0.0 78-79 0.0 0.0 0.0 4.732416139614788 0.0 80-81 0.0 0.0 0.0 5.100257333527667 0.0 82-83 0.0 0.0 0.0 5.473564176879168 0.0 84-85 0.0 0.0 0.0 5.835850120542958 0.0 86-87 0.0 0.0 0.0 6.2016006288782215 0.0 88-89 0.0 0.0 0.0 6.594679384406591 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9685 0.0 36.837677 1 GTATCAA 15095 0.0 34.964947 1 CGTAAGG 85 6.403379E-4 27.94493 2 TATCAAC 18935 0.0 27.748644 2 ATCAACG 18990 0.0 27.662489 3 TCAACGC 19100 0.0 27.523933 4 CAACGCA 19185 0.0 27.327728 5 CTATCGT 70 0.008401826 27.136774 4 GTACATG 12135 0.0 26.933983 1 AACGCAG 19590 0.0 26.738516 6 TACATGG 12205 0.0 26.078882 2 ACATGGG 12100 0.0 25.39686 3 GAGTACT 11775 0.0 23.97586 12-13 ACGCAGA 21810 0.0 23.907269 7 CATGGGG 3890 0.0 23.805716 4 GGGTACG 100 0.0016526673 23.753191 1 CGCAGAG 21980 0.0 23.700756 8 AGTACTT 11955 0.0 22.939592 12-13 GCAGAGT 22600 0.0 22.798412 9 ATGGGAG 2825 0.0 22.694027 5 >>END_MODULE