FastQCFastQC Report
Thu 26 May 2016
SRR938019_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938019_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences697287
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110511.5848567376130631No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95951.3760474524836976No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57470.8241943417846598No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31760.4554795944854845No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20430.292992698845669No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15380.2205691487149481No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9240.1325135847936359No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8080.11587768020915347No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7970.11430013753303878No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7840.11243576891581228No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7640.10956750950469461No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7250.10397440365301519No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7140.10239686097690047No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50650.049.3828541
GTATCAA83450.037.8093721
TATCAAC103200.030.6239072
ATCAACG104700.030.0943853
TCAACGC105900.029.7084964
CAACGCA106450.029.5996465
AACGCAG108950.029.0035216
GTGTAGC5500.026.9044041
ACGCAGA122100.025.6426987
CGCAGAG121550.025.4851158
CATTCCG1501.0569765E-625.3391889
CGAGACA950.001222371625.0129574
GCAGAGT122450.024.4441979
TCGAGAC1201.7038768E-423.7623123
GCGTGCC500.001655741523.74526878-79
GAGTACT79700.023.54515612-13
CATGGGT14550.023.517344
TAGCCCT9150.023.3727654
GTACTAG1252.1809789E-422.9121341
GTACATG68800.022.6873441
Produced by FastQC (version 0.11.5)