##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938019_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 697287 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.957825113618927 33.0 31.0 34.0 30.0 34.0 2 32.06870772006362 33.0 31.0 34.0 30.0 34.0 3 32.108762962739874 34.0 31.0 34.0 30.0 34.0 4 35.64714816137401 37.0 35.0 37.0 35.0 37.0 5 35.700578097684314 37.0 35.0 37.0 35.0 37.0 6 35.7817139857763 37.0 36.0 37.0 35.0 37.0 7 35.735454697993795 37.0 36.0 37.0 35.0 37.0 8 35.72952887405042 37.0 36.0 37.0 35.0 37.0 9 37.3745502210711 39.0 38.0 39.0 35.0 39.0 10-11 37.302325297904595 39.0 37.0 39.0 35.0 39.0 12-13 37.194738321523275 39.0 37.0 39.0 34.5 39.0 14-15 38.311579736894565 40.0 38.0 41.0 34.0 41.0 16-17 38.18264072039203 40.0 38.0 41.0 34.0 41.0 18-19 38.47518166838045 40.0 38.0 41.0 34.0 41.0 20-21 38.52841082653197 40.0 38.5 41.0 34.5 41.0 22-23 38.414099215961286 40.0 38.0 41.0 34.0 41.0 24-25 38.30725440170259 40.0 38.0 41.0 34.0 41.0 26-27 38.1130130061223 40.0 38.0 41.0 34.0 41.0 28-29 37.90559482680733 40.0 38.0 41.0 34.0 41.0 30-31 37.53425060269301 40.0 37.0 41.0 33.0 41.0 32-33 37.06657875451572 39.5 35.5 41.0 33.0 41.0 34-35 36.74473064892935 39.0 35.0 41.0 31.5 41.0 36-37 36.430045304157396 39.0 35.0 41.0 31.0 41.0 38-39 36.077488179185906 39.0 35.0 41.0 30.0 41.0 40-41 35.775828317464686 39.0 35.0 41.0 30.0 41.0 42-43 35.35178628025476 39.0 35.0 41.0 27.0 41.0 44-45 34.69524313517963 39.0 35.0 40.5 23.0 41.0 46-47 33.83386252719468 38.0 34.5 40.0 10.0 41.0 48-49 33.064281995792264 38.0 33.0 40.0 2.0 41.0 50-51 32.14476320367366 37.5 32.5 39.5 2.0 40.5 52-53 32.08497505331377 38.0 33.0 40.0 2.0 40.5 54-55 32.077427228673415 38.0 32.5 40.0 2.0 41.0 56-57 31.67506493022242 38.0 31.5 40.0 2.0 41.0 58-59 31.318943275867756 38.0 31.0 40.0 2.0 41.0 60-61 30.881384566182934 37.0 30.5 40.0 2.0 41.0 62-63 30.375704695484067 36.5 29.0 40.0 2.0 41.0 64-65 29.892400116451334 36.0 28.0 39.0 2.0 41.0 66-67 29.331116885873392 35.5 27.5 39.0 2.0 40.5 68-69 28.727399908502527 35.0 26.0 38.5 2.0 40.0 70-71 28.047719231822764 35.0 25.0 38.0 2.0 40.0 72-73 27.346217554608074 34.0 22.0 37.0 2.0 39.5 74-75 26.62639773866428 34.0 19.0 36.5 2.0 39.0 76-77 25.853513689485105 33.0 13.0 36.0 2.0 39.0 78-79 25.100098668123742 32.5 4.5 35.5 2.0 37.5 80-81 24.342802174714286 32.0 2.0 35.0 2.0 37.0 82-83 23.55457508888019 31.0 2.0 35.0 2.0 36.5 84-85 22.772892653957406 31.0 2.0 35.0 2.0 36.0 86-87 22.02151911623191 30.0 2.0 34.0 2.0 35.5 88-89 21.222905346005305 29.0 2.0 34.0 2.0 35.0 90-91 20.39574522398955 29.0 2.0 34.0 2.0 35.0 92-93 19.521400800531204 26.5 2.0 34.0 2.0 35.0 94-95 18.614348180878174 25.5 2.0 34.0 2.0 35.0 96-97 17.53579946277501 22.0 2.0 33.5 2.0 35.0 98-99 16.289307702567235 3.5 2.0 33.0 2.0 35.0 100-101 13.282885669745744 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5483038137330887 1101 2 0.6255388971485232 1101 3 0.5358435576913507 1101 4 0.5229110396696584 1101 5 0.3344641641328039 1101 6 0.34016208007332693 1101 7 0.3596312718970225 1101 8 0.4755906843119888 1101 9 0.40225262481350654 1101 10-11 0.5137938081441078 1101 12-13 0.49567696075143886 1101 14-15 0.8251535204122504 1101 16-17 0.6350194337713049 1101 18-19 0.5955446319270408 1101 20-21 0.640245965922972 1101 22-23 0.769155790650224 1101 24-25 0.6590505793595085 1101 26-27 0.728633590522584 1101 28-29 0.736268830774705 1101 30-31 0.8605179888156158 1101 32-33 0.976567780745242 1101 34-35 0.9436378543211674 1101 36-37 0.9231263388966866 1101 38-39 1.339834202898487 1101 40-41 1.2356583692346916 1101 42-43 1.4109595608544296 1101 44-45 1.5907190400358218 1101 46-47 1.893849839590139 1101 48-49 2.1861245957032978 1101 50-51 2.3117053636410887 1101 52-53 2.2667814737476775 1101 54-55 2.384148616951535 1101 56-57 2.4671533208509757 1101 58-59 2.6150114104171998 1101 60-61 2.671781723657947 1101 62-63 2.488570631127626 1101 64-65 2.525705133432762 1101 66-67 2.8390492732488433 1101 68-69 3.1236146934639706 1101 70-71 3.2734318558793447 1101 72-73 3.3791390316085703 1101 74-75 3.456972596994987 1101 76-77 3.3331192343452507 1101 78-79 3.5925599115914046 1101 80-81 3.9018521180442782 1101 82-83 3.8627752602034846 1101 84-85 4.3465607378548015 1101 86-87 4.551354873376663 1101 88-89 4.787699187396235 1101 90-91 5.275476042572841 1101 92-93 5.268965174554701 1101 94-95 5.331417700200177 1101 96-97 5.284662474343165 1101 98-99 5.120134539704033 1101 100-101 4.6622060740109035 1102 1 0.6181364126039419 1102 2 0.6356561764929936 1102 3 0.5436687509277291 1102 4 0.46078255331939744 1102 5 0.4929700844166405 1102 6 0.47169515904329984 1102 7 0.5649053226101302 1102 8 0.4393509444947341 1102 9 0.7914510986333241 1102 10-11 0.6728248808347743 1102 12-13 0.744510502925543 1102 14-15 0.8957379460532024 1102 16-17 0.9487128179773165 1102 18-19 0.8598666503144159 1102 20-21 0.7504139302182367 1102 22-23 0.756654910703169 1102 24-25 0.6378164008076865 1102 26-27 0.707043209399913 1102 28-29 0.9563231763052755 1102 30-31 0.7332241428133912 1102 32-33 0.8165370881917084 1102 34-35 0.9449077939216011 1102 36-37 1.0554731929099432 1102 38-39 1.0168147877465046 1102 40-41 1.290234797916746 1102 42-43 1.5628158971772308 1102 44-45 1.4304630810343113 1102 46-47 1.2399055648777093 1102 48-49 1.0958575141709233 1102 50-51 1.3181902215120331 1102 52-53 1.3404154439280305 1102 54-55 1.263578974421229 1102 56-57 1.5078997975429331 1102 58-59 1.641609746261313 1102 60-61 1.8502955282674698 1102 62-63 1.8198685882425245 1102 64-65 1.9749216285684206 1102 66-67 2.4018310322982224 1102 68-69 2.0936134263402018 1102 70-71 2.1091506670356672 1102 72-73 2.4280950624137567 1102 74-75 2.6670687576099645 1102 76-77 2.855537469664217 1102 78-79 3.163114771011063 1102 80-81 3.0521315892312906 1102 82-83 3.314217669428146 1102 84-85 3.5605568238502663 1102 86-87 3.836166283122253 1102 88-89 3.6010448165231885 1102 90-91 3.7497928516736074 1102 92-93 3.742330232912792 1102 94-95 4.193533515312737 1102 96-97 4.444237002339559 1102 98-99 4.8016795859540515 1102 100-101 4.397754527415945 1103 1 0.3576988507542005 1103 2 0.18349912495377652 1103 3 0.23305332927964884 1103 4 0.12779959041586864 1103 5 0.18575673289613803 1103 6 0.20072508763192332 1103 7 0.03581922776940161 1103 8 0.08924797303254195 1103 9 0.1950805083527598 1103 10-11 0.12734358954589453 1103 12-13 0.2548798628977238 1103 14-15 0.45431667241657436 1103 16-17 0.48442786785829384 1103 18-19 0.4635201312847528 1103 20-21 0.4337285414675378 1103 22-23 0.5264414789451664 1103 24-25 0.41999565138940653 1103 26-27 0.3713495561864022 1103 28-29 0.4661429093818583 1103 30-31 0.7578664172791392 1103 32-33 0.8863236564337029 1103 34-35 0.764369400276415 1103 36-37 0.9855288242453639 1103 38-39 1.1575500307324091 1103 40-41 1.2272603936802042 1103 42-43 0.9430132814346379 1103 44-45 1.1358376564442025 1103 46-47 1.7136785997275226 1103 48-49 1.947185444845715 1103 50-51 1.7961195590177184 1103 52-53 1.7087991931590878 1103 54-55 1.9193172931780822 1103 56-57 2.0017362200201347 1103 58-59 2.1613842788778577 1103 60-61 2.308830124004178 1103 62-63 2.338264373099033 1103 64-65 2.342008204834169 1103 66-67 2.4338417841236257 1103 68-69 2.645174045773725 1103 70-71 2.883096212516957 1103 72-73 3.2217469386340376 1103 74-75 3.39771814646765 1103 76-77 3.507063319513101 1103 78-79 3.356651640143692 1103 80-81 3.585328518809508 1103 82-83 3.6341526770507073 1103 84-85 3.773834793306527 1103 86-87 3.9138268249928565 1103 88-89 4.091977978683179 1103 90-91 4.512019709840448 1103 92-93 4.696550725571058 1103 94-95 4.834517494557971 1103 96-97 5.037567928547801 1103 98-99 5.154104095836637 1103 100-101 5.0045479693373665 1104 1 0.4939875483178575 1104 2 0.3141243920967156 1104 3 0.348123244404519 1104 4 0.30629687319049026 1104 5 0.2857139900250374 1104 6 0.35495052574634656 1104 7 0.22295667663060925 1104 8 0.2260862217960522 1104 9 0.3306059403610675 1104 10-11 0.398837147584608 1104 12-13 0.4056324427353033 1104 14-15 0.6137170509962928 1104 16-17 0.5779736943222389 1104 18-19 0.5638208579135622 1104 20-21 0.5938998182581585 1104 22-23 0.5277084997670514 1104 24-25 0.4387948820177314 1104 26-27 0.5739736631657024 1104 28-29 0.6037138930785062 1104 30-31 0.634965397556364 1104 32-33 0.6360183502115575 1104 34-35 0.5505252896334412 1104 36-37 0.683558072581441 1104 38-39 0.46392482465071083 1104 40-41 0.5632193605239522 1104 42-43 0.8019694394611392 1104 44-45 0.6896631434076212 1104 46-47 0.6485949458226585 1104 48-49 0.45220009954437757 1104 50-51 0.4843332123119737 1104 52-53 0.28441591172780534 1104 54-55 0.38145932163262586 1104 56-57 0.6991090599786105 1104 58-59 0.6311284322636972 1104 60-61 1.0007074519641215 1104 62-63 0.8196177955817809 1104 64-65 0.7698109159534177 1104 66-67 1.1410378517794832 1104 68-69 1.184421160629924 1104 70-71 1.190699863979372 1104 72-73 1.5219850774873045 1104 74-75 1.6810117899921089 1104 76-77 1.714503632146677 1104 78-79 2.04365170120494 1104 80-81 2.141329312605688 1104 82-83 2.2383002620784893 1104 84-85 2.473955389264269 1104 86-87 2.639119918966113 1104 88-89 2.8830111349389043 1104 90-91 3.04771763986426 1104 92-93 3.464930160143936 1104 94-95 3.3777946515464308 1104 96-97 3.6208859965700277 1104 98-99 3.5058702923317178 1104 100-101 2.971284856435126 1105 1 0.5874044880233491 1105 2 0.5552459226205784 1105 3 0.2660891992872081 1105 4 0.2577242072017114 1105 5 0.2671473497207373 1105 6 0.3433040074621658 1105 7 0.38037768367313163 1105 8 0.3892692896226606 1105 9 0.40164311130219943 1105 10-11 0.4385125138332384 1105 12-13 0.3911849226810702 1105 14-15 0.6277973809025141 1105 16-17 0.7290018267366563 1105 18-19 0.5335935766270836 1105 20-21 0.551446988069479 1105 22-23 0.6040605127222705 1105 24-25 0.6121792453665051 1105 26-27 0.6634692003931377 1105 28-29 0.7139603812342656 1105 30-31 0.8667941927960712 1105 32-33 0.7940325105700836 1105 34-35 0.9495621216298247 1105 36-37 1.1548842880749817 1105 38-39 1.1359863619534636 1105 40-41 1.1332396972090706 1105 42-43 1.200897997439526 1105 44-45 1.5128045963507901 1105 46-47 1.586045516379663 1105 48-49 1.6647890725417795 1105 50-51 1.6914284557872818 1105 52-53 1.8849977668854123 1105 54-55 1.999416802896512 1105 56-57 2.197965121440596 1105 58-59 2.1425772327758814 1105 60-61 2.0553952461869933 1105 62-63 2.000859086584679 1105 64-65 2.0694804448202113 1105 66-67 2.294772834643947 1105 68-69 2.4837517447447155 1105 70-71 2.595654106437852 1105 72-73 2.8316581841042634 1105 74-75 2.7297821737528842 1105 76-77 2.9757594224528567 1105 78-79 2.963571295977655 1105 80-81 3.3111123879644033 1105 82-83 3.4743799894162635 1105 84-85 3.77149239073303 1105 86-87 4.1526259258203275 1105 88-89 4.335728342034148 1105 90-91 4.4327777084064195 1105 92-93 4.723286089794069 1105 94-95 4.632855284996509 1105 96-97 4.861296684627874 1105 98-99 4.811386618170609 1105 100-101 4.432461559632504 1106 1 0.404464062567957 1106 2 0.4580843898302973 1106 3 0.3904485886166569 1106 4 0.26653541361771005 1106 5 0.3527149532524163 1106 6 0.15753140851591496 1106 7 0.2140751535981451 1106 8 0.042818047961837635 1106 9 0.2148770038774117 1106 10-11 0.27417002763446163 1106 12-13 0.2751647108524722 1106 14-15 0.5279048236746249 1106 16-17 0.4120861086233347 1106 18-19 0.3685902499471041 1106 20-21 0.40767821930071335 1106 22-23 0.41280113957666487 1106 24-25 0.2959468453017706 1106 26-27 0.3820860208063621 1106 28-29 0.34425160059734594 1106 30-31 0.5144628195066119 1106 32-33 0.5669776113594978 1106 34-35 0.6634991125129233 1106 36-37 0.4435232264145199 1106 38-39 0.5863963527826073 1106 40-41 0.7509637086456564 1106 42-43 0.3400948290016217 1106 44-45 0.8732571147374415 1106 46-47 0.6813234290228465 1106 48-49 1.070315856811085 1106 50-51 1.4050440174567385 1106 52-53 1.337046372377138 1106 54-55 1.4893266228961508 1106 56-57 1.2556065952497306 1106 58-59 1.6126146354480149 1106 60-61 1.8527599761864586 1106 62-63 1.8280672828048559 1106 64-65 2.0470433370537613 1106 66-67 2.021286024480485 1106 68-69 2.004127659581709 1106 70-71 2.4326894951199485 1106 72-73 2.8031341510892105 1106 74-75 2.9562256831305262 1106 76-77 2.627912323857249 1106 78-79 3.0728459898331906 1106 80-81 3.230689899607782 1106 82-83 3.2427126933777117 1106 84-85 3.5114291838135365 1106 86-87 3.547606235471296 1106 88-89 3.49451886908815 1106 90-91 3.566890114225412 1106 92-93 3.891002458857745 1106 94-95 4.275805870312368 1106 96-97 4.414656181849647 1106 98-99 3.972065260885177 1106 100-101 3.5004224846293575 1107 1 0.5255342982057165 1107 2 0.26967215361836594 1107 3 0.3081303067536112 1107 4 0.20674876809510323 1107 5 0.2549259654786056 1107 6 0.3148359057293604 1107 7 0.30767862049447814 1107 8 0.3318878461363397 1107 9 0.2943016485524339 1107 10-11 0.35500047872287865 1107 12-13 0.38517191076862645 1107 14-15 0.5320037632819137 1107 16-17 0.527489827972424 1107 18-19 0.5335708602994913 1107 20-21 0.5630175495262293 1107 22-23 0.44707365184614645 1107 24-25 0.5059599691995658 1107 26-27 0.5279526765364437 1107 28-29 0.6445108887170221 1107 30-31 0.5296361871079043 1107 32-33 0.5876842056624554 1107 34-35 0.7791056143106232 1107 36-37 0.6701360304173107 1107 38-39 0.7636475797303532 1107 40-41 0.9770467719821383 1107 42-43 0.8593546047105605 1107 44-45 0.9301432877085958 1107 46-47 1.4279239749405335 1107 48-49 1.0868130064645314 1107 50-51 0.9113917916345571 1107 52-53 0.9491702565040541 1107 54-55 0.7630886246551185 1107 56-57 0.7548717936803371 1107 58-59 0.9289233569160764 1107 60-61 1.0132132974895534 1107 62-63 1.070120033582949 1107 64-65 1.2018871034301561 1107 66-67 1.5149701486653697 1107 68-69 1.6386328020534684 1107 70-71 1.7528545007933012 1107 72-73 1.5293265802406673 1107 74-75 1.840785508509775 1107 76-77 1.905580735820049 1107 78-79 1.8554472557425008 1107 80-81 1.98937308962633 1107 82-83 2.4052496613016316 1107 84-85 2.248488701601431 1107 86-87 1.9864741625789826 1107 88-89 2.638680101640883 1107 90-91 2.7502054477683657 1107 92-93 2.8858747970532974 1107 94-95 2.9024651547865226 1107 96-97 3.379761651507472 1107 98-99 3.3717870615933236 1107 100-101 3.0385752048761603 1108 1 0.294967418588552 1108 2 0.2595661223465129 1108 3 0.3089580071260727 1108 4 0.12453290954623952 1108 5 0.1257999504753471 1108 6 0.06294622772383462 1108 7 0.0506562487999247 1108 8 0.21207821722200748 1108 9 0.1135656611867617 1108 10-11 0.20609646818159177 1108 12-13 0.20303144906403503 1108 14-15 0.4617681540896825 1108 16-17 0.5080174631753707 1108 18-19 0.4789176619986577 1108 20-21 0.38961313227010663 1108 22-23 0.36161358838911895 1108 24-25 0.36506062993279187 1108 26-27 0.525510009057939 1108 28-29 0.5848213239256808 1108 30-31 0.44627629442353367 1108 32-33 0.44249890067389686 1108 34-35 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3.6064227869948553 2316 96-97 4.042818301815046 2316 98-99 3.9429864162325643 2316 100-101 4.221262762846685 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4437.0 3 690.0 4 642.0 5 598.0 6 634.0 7 748.0 8 943.0 9 1218.0 10 1655.0 11 2446.0 12 4069.0 13 5995.0 14 7323.0 15 10474.0 16 14169.0 17 20210.0 18 18253.0 19 10546.0 20 9191.0 21 8601.0 22 8996.0 23 9575.0 24 10939.0 25 12172.0 26 13721.0 27 15650.0 28 17896.0 29 20588.0 30 23766.0 31 26963.0 32 31519.0 33 37347.0 34 45404.0 35 59270.0 36 71306.0 37 94921.0 38 69242.0 39 5170.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.756176505737 19.63595367782678 15.291846105334997 19.316023711101224 2 15.891140380825053 21.999718842434135 40.923774741148556 21.185366035592253 3 18.928535560051078 28.034835506965667 31.68079886368528 21.355830069297983 4 11.316393915352645 19.414288850025752 38.09331839382067 31.17599884080094 5 11.130689824394247 39.72638907622593 34.14899081936915 14.993930280010675 6 27.36205333057842 38.278694169909436 18.442869933537658 15.916382565974493 7 24.381429766258137 37.61116474978158 20.694038169373545 17.31336731458674 8 25.92671761419798 33.74099965999911 20.044702226647175 20.287580499155737 9 27.40549667952582 15.822213678144264 21.220620367009595 35.55166927532032 10-11 23.82354164783788 28.129630984505166 26.73529669144176 21.31153067621519 12-13 26.280827292563576 27.450869282667856 27.28424302328751 18.98406040148106 14-15 23.028983293131557 27.37974074337065 29.153783091154388 20.4374928723434 16-17 18.560075170172937 30.43940122079804 29.894632648816145 21.105890960212886 18-19 17.859566123615895 27.19932693645632 35.79566238375237 19.145444556175413 20-21 20.87397827248697 25.664575689430702 35.85218978756676 17.60925625051557 22-23 19.53039716204217 26.900781930253142 35.27285794001329 18.295962967691402 24-25 18.515978525045984 26.765141363389848 36.30297360968037 18.415906501883793 26-27 18.455748067192413 27.066120741475057 35.912418602582626 18.565712588749903 28-29 18.053348960212425 27.443750049312175 35.872223007232215 18.630677983243185 30-31 18.89481148011939 27.118055630275368 35.97898854957725 18.00814434002799 32-33 18.02526590021605 27.30781590265484 36.117168245187685 18.549749951941426 34-35 18.53329986012983 27.622135351289316 35.780870064196826 18.063694724384032 36-37 18.668426413416157 27.43469092056752 35.1358543618288 18.761028304187526 38-39 18.21975763217667 27.684323691644785 35.39607280071317 18.699845875465368 40-41 19.11383245518352 27.246453515431984 35.06532158068401 18.57439244870049 42-43 18.545355969543955 27.444146347935284 34.837919883993365 19.1725777985274 44-45 19.302498198156204 27.767901004371037 34.07037410221564 18.859226695257117 46-47 19.154000849169194 27.824348205269438 33.171819058110714 19.849831887450655 48-49 19.154563716465724 27.87937379021298 32.97835489763382 19.987707595687475 50-51 19.613086722015804 28.490319638043317 31.695449222293696 20.201144417647182 52-53 19.50110289161257 28.849149095456777 31.47457612533668 20.175171887593976 54-55 19.85279459367206 28.468842908712283 31.36296946463069 20.315393032984968 56-57 19.909757311421895 28.816633224856613 30.813173826178126 20.460435637543366 58-59 19.854660605838962 28.781473888316413 31.034277523092022 20.3295879827526 60-61 19.99427751472034 28.450217605032346 31.085357537007912 20.4701473432394 62-63 19.61742285710188 28.25807284383538 31.369891501552537 20.754612797510198 64-65 19.599745278107257 28.416694036840013 31.043872116988485 20.939688568064245 66-67 19.60527985876012 28.609393857470778 30.724549966403895 21.060776317365203 68-69 20.065658083165474 28.257213891460502 30.69314598740621 20.983982037967813 70-71 19.8864129425273 28.118139719682805 30.713616175003533 21.28183116278636 72-73 19.868558917203163 27.96601235379797 30.957071520214228 21.208357208784637 74-75 20.060468545169257 28.078144407376705 30.418073440023406 21.443313607430632 76-77 19.67245090994623 28.0919455165302 30.747276041644255 21.48832753187931 78-79 19.836827128834425 28.062162596308855 30.277337945831828 21.823672329024895 80-81 19.86492097561535 28.241383835696404 30.003377692741832 21.89031749594641 82-83 20.200902461833266 28.41772278988646 29.918646479366156 21.46272826891412 84-85 20.393516825361313 28.117636260112928 29.699204447178996 21.789642467346766 86-87 20.16798045558227 28.14565381225857 29.76773435768952 21.918631374469637 88-89 20.847931846430694 27.828680815599355 29.710750410790503 21.612636927179448 90-91 20.94291867871469 27.91870381672929 29.47868800961623 21.659689494939787 92-93 20.97072158324046 27.7561915252803 29.70720148937971 21.565885402099532 94-95 21.066851616494006 27.903420615498494 29.240625080688424 21.78910268731908 96-97 21.45454910569887 27.60503439288719 29.043217203400346 21.897199298013597 98-99 21.663165640264694 27.285773401713914 28.790844212333607 22.260216745687785 100-101 22.458035914251422 26.541530519074065 28.98421932156865 22.016214245105864 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3003.0 1 1700.5 2 507.0 3 641.0 4 477.5 5 357.0 6 655.5 7 1324.5 8 1956.0 9 2080.5 10 1751.0 11 1438.0 12 1495.0 13 1659.5 14 1800.0 15 1992.0 16 2203.0 17 2433.5 18 2732.0 19 3041.0 20 3378.0 21 3765.0 22 4229.5 23 4787.0 24 5518.5 25 6346.0 26 7557.0 27 9038.5 28 10062.0 29 11144.5 30 12481.0 31 13904.5 32 15254.5 33 16794.0 34 18869.5 35 20873.0 36 22593.5 37 23944.5 38 23773.0 39 22751.0 40 22520.5 41 22575.0 42 22744.5 43 22987.0 44 23029.5 45 23270.0 46 23603.5 47 23976.0 48 24519.0 49 24690.0 50 24601.0 51 23415.0 52 20755.5 53 17995.0 54 16035.5 55 14559.0 56 12661.5 57 10435.0 58 8525.5 59 6795.0 60 5021.0 61 3628.0 62 2599.5 63 1855.5 64 1348.5 65 997.5 66 774.0 67 592.0 68 432.5 69 321.0 70 231.0 71 185.0 72 169.5 73 163.0 74 148.5 75 104.5 76 67.5 77 43.0 78 31.0 79 21.5 80 18.0 81 11.0 82 3.5 83 4.0 84 4.5 85 2.0 86 1.0 87 1.5 88 1.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3958197987342371 2 0.02423679202394423 3 0.04259365225509726 4 0.036426894521194284 5 0.055213993664014964 6 0.05033795266511494 7 0.033845461051188394 8 0.03298498322785309 9 0.03556641669785899 10-11 0.030977201640070732 12-13 0.0312640275811825 14-15 0.025599215244225117 16-17 0.028969420052288367 18-19 0.02524068281783541 20-21 0.0350644713009134 22-23 0.02380655311227658 24-25 0.04144634849065019 26-27 0.03499276481563546 28-29 0.02832406168478689 30-31 0.034203993477578104 32-33 0.03169426649285015 34-35 0.0304035497578472 36-37 0.031120614610626617 38-39 0.01771150186365155 40-41 0.01183157007086035 42-43 0.012261808982527999 44-45 0.012118396011972114 46-47 0.019360751025044205 48-49 0.015560307305313308 50-51 0.01097109224752505 52-53 0.004158976146120607 54-55 0.004302389116676491 56-57 0.008031126351129448 58-59 0.0032267918375073674 60-61 0.005377986395845613 62-63 0.006023344763347087 64-65 0.008031126351129448 66-67 0.004804334513622081 68-69 0.005521399366401497 70-71 0.01613395918753684 72-73 0.013265699776419178 74-75 0.009321843086132396 76-77 0.0068838225866823845 78-79 0.007314061498350034 80-81 0.009393549571410338 82-83 0.01190327655613829 84-85 0.005162866940011788 86-87 5.019453969455905E-4 88-89 0.022300716921439808 90-91 0.0190022185986545 92-93 0.012046689526694174 94-95 0.023089488259497165 96-97 0.01756808889309567 98-99 0.015847133246425074 100-101 0.10598218524079754 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 697287.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.73077973508256 #Duplication Level Percentage of deduplicated Percentage of total 1 78.84233817195032 49.45841349663507 2 12.771158082080921 16.022894092178753 3 4.2322063917882655 7.964688208700345 4 1.7333976232224917 4.34949537982743 5 0.8897813568758646 2.790833915528137 6 0.47799164479274436 1.7990873150822082 7 0.268910197126178 1.1808262441107928 8 0.17256740885246846 0.8660230491342507 9 0.1061571467357397 0.5993388530266074 >10 0.4445818505966634 4.859315423205773 >50 0.03366096376799024 1.5092734992281345 >100 0.023814393865090495 2.8842238137980853 >500 0.0020608610072236884 0.9537948165713666 >1k 6.869536690745628E-4 0.9706011408995068 >5k 4.579691127163752E-4 2.203783143951492 >10k+ 2.289845563581876E-4 1.5874076081220139 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11051 1.5848567376130631 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9595 1.3760474524836976 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5747 0.8241943417846598 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3176 0.4554795944854845 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2043 0.292992698845669 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1538 0.2205691487149481 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 924 0.1325135847936359 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 808 0.11587768020915347 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 797 0.11430013753303878 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 784 0.11243576891581228 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 764 0.10956750950469461 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 725 0.10397440365301519 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 714 0.10239686097690047 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 7.170648527794151E-5 0.0 16-17 0.0 0.0 0.0 1.4341297055588302E-4 0.0 18-19 0.0 0.0 0.0 2.8682594111176604E-4 0.0 20-21 0.0 0.0 0.0 6.453583675014735E-4 0.0 22-23 0.0 0.0 0.0 0.0022229010436161864 0.0 24-25 0.0 0.0 0.0 0.006381877189736794 0.0 26-27 0.0 0.0 0.0 0.014413003540866243 0.0 28-29 0.0 0.0 0.0 0.03793273071203106 0.0 30-31 0.0 0.0 0.0 0.0979510588896681 0.0 32-33 0.0 0.0 0.0 0.20178204957212742 0.0 34-35 0.0 0.0 0.0 0.36247628307999435 0.0 36-37 0.0 0.0 0.0 0.588423418190788 0.0 38-39 0.0 0.0 0.0 0.8783327381695055 0.0 40-41 0.0 0.0 0.0 1.2063899083160878 0.0 42-43 0.0 0.0 0.0 1.4950085115598024 0.0 44-45 0.0 0.0 0.0 1.827870016220007 0.0 46-47 0.0 0.0 0.0 2.1839644221102645 0.0 48-49 0.0 0.0 0.0 2.5655863367594693 0.0 50-51 0.0 0.0 0.0 2.9540920739953562 0.0 52-53 0.0 0.0 0.0 3.309182589091723 0.0 54-55 0.0 0.0 0.0 3.6904459713145377 0.0 56-57 0.0 0.0 0.0 4.097021742840466 0.0 58-59 0.0 0.0 0.0 4.534861541947577 0.0 60-61 0.0 0.0 0.0 4.982740248993599 0.0 62-63 0.0 0.0 0.0 5.413122573631805 0.0 64-65 0.0 0.0 0.0 5.85182285056225 0.0 66-67 0.0 0.0 0.0 6.323436404235272 0.0 68-69 0.0 0.0 0.0 6.836854838825333 0.0 70-71 0.0 0.0 0.0 7.3861265160543645 0.0 72-73 0.0 0.0 0.0 7.940345940767575 0.0 74-75 0.0 0.0 0.0 8.464018402752382 0.0 76-77 0.0 0.0 0.0 9.006047724968342 0.0 78-79 0.0 0.0 0.0 9.592463361571347 0.0 80-81 0.0 0.0 0.0 10.218962923444721 0.0 82-83 0.0 0.0 0.0 10.852131188448947 0.0 84-85 0.0 0.0 0.0 11.470241091544802 0.0 86-87 0.0 0.0 0.0 12.067054168513108 0.0 88-89 0.0 0.0 0.0 12.71076328685319 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5065 0.0 49.382854 1 GTATCAA 8345 0.0 37.809372 1 TATCAAC 10320 0.0 30.623907 2 ATCAACG 10470 0.0 30.094385 3 TCAACGC 10590 0.0 29.708496 4 CAACGCA 10645 0.0 29.599646 5 AACGCAG 10895 0.0 29.003521 6 GTGTAGC 550 0.0 26.904404 1 ACGCAGA 12210 0.0 25.642698 7 CGCAGAG 12155 0.0 25.485115 8 CATTCCG 150 1.0569765E-6 25.339188 9 CGAGACA 95 0.0012223716 25.012957 4 GCAGAGT 12245 0.0 24.444197 9 TCGAGAC 120 1.7038768E-4 23.762312 3 GCGTGCC 50 0.0016557415 23.745268 78-79 GAGTACT 7970 0.0 23.545156 12-13 CATGGGT 1455 0.0 23.51734 4 TAGCCCT 915 0.0 23.372765 4 GTACTAG 125 2.1809789E-4 22.912134 1 GTACATG 6880 0.0 22.687344 1 >>END_MODULE