FastQCFastQC Report
Thu 26 May 2016
SRR938019_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938019_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences697287
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102521.4702697741389126No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93161.336035233698606No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57680.8272060141663331No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30410.4361188434604402No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT21940.31464805739960733No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20810.29844239172679254No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16310.2339065549766452No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT13190.18916170816320968RNA PCR Primer, Index 26 (100% over 22bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8650.12405221953083881No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT7750.11114505218080935RNA PCR Primer, Index 26 (100% over 24bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7690.11028457435747405No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7600.10899385762247109No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7390.10598218524079754No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7230.10368757771190343No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51650.047.683451
CATTCCG1253.6379788E-1241.7949189
GTATCAA83200.036.344781
GTGTAGC6150.030.9238171
TATCAAC101150.029.8010542
ATCAACG103650.029.0759983
TCAACGC105150.028.6591644
CAACGCA106650.028.2115125
AACGCAG107750.027.9455596
CGGCGTC350.00839715127.13955786-87
GTAGCCC6850.027.0637973
TAGCCCT10850.026.7229024
TCGAGAC1257.3431456E-626.6196733
TCGTATC751.0615076E-625.33025494-95
CGCAGAG119600.024.8590188
ACGCAGA121350.024.6179397
GAGTACT74800.024.44555712-13
CCTGTAC2551.8189894E-1124.2335973
GCAGAGT122450.023.698639
GTATAGC6250.023.5825041
Produced by FastQC (version 0.11.5)