FastQCFastQC Report
Thu 26 May 2016
SRR938018_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938018_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences698767
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109781.5710530119481887No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93891.3436524621225674No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58150.8321801115393258No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33420.4782710116533837No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20670.29580675675869067No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14730.21079988036069247No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9740.139388379817593No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8470.12121350893788631No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8230.11777888766928031No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7570.10833367918061385No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7550.10804746074156335No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7140.10217998274102813No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52150.047.8124541
GTATCAA87150.038.6763651
CATTCCG1500.038.001039
TAACGCA600.00394107931.6811314
TATCAAC106600.031.6514112
ATCAACG109400.030.7978823
TCAACGC110000.030.5866934
CAACGCA111600.030.2333375
AACGCAG113100.029.7860836
GTGTAGC6500.027.138261
CGCAGAG125650.026.5387238
ACGCAGA126700.026.4687527
GCAGAGT127300.025.6350239
GACGTGG2600.025.5776187
ACGAAGG951.1794327E-825.02396494-95
GAGTACT78750.024.88162812-13
GTGTCGA500.001655630223.74554686-87
GTACTTT84250.023.14454314-15
AGTACTT79300.023.06178512-13
CATGGGT13900.022.9061414
Produced by FastQC (version 0.11.5)