##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938018_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 698767 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.960315813425648 33.0 31.0 34.0 31.0 34.0 2 32.103802841290445 34.0 31.0 34.0 30.0 34.0 3 32.134079027773204 34.0 31.0 34.0 30.0 34.0 4 35.654396959215305 37.0 35.0 37.0 35.0 37.0 5 35.70520788760774 37.0 35.0 37.0 35.0 37.0 6 35.779908038015535 37.0 37.0 37.0 35.0 37.0 7 35.7376249880146 37.0 36.0 37.0 35.0 37.0 8 35.72973394564998 37.0 36.0 37.0 35.0 37.0 9 37.380464446661044 39.0 38.0 39.0 35.0 39.0 10-11 37.320316357240685 39.0 37.5 39.0 35.0 39.0 12-13 37.238147336665875 39.0 37.0 39.0 35.0 39.0 14-15 38.37717579679636 40.0 38.0 41.0 34.0 41.0 16-17 38.26436709232119 40.0 38.0 41.0 34.0 41.0 18-19 38.509509607637455 40.0 38.5 41.0 34.5 41.0 20-21 38.551635953043004 40.0 38.5 41.0 35.0 41.0 22-23 38.44731290973958 40.0 38.0 41.0 34.0 41.0 24-25 38.355797426037576 40.0 38.0 41.0 34.0 41.0 26-27 38.178319096351146 40.0 38.0 41.0 34.0 41.0 28-29 37.96111221623231 40.0 38.0 41.0 34.0 41.0 30-31 37.61650163788502 40.0 37.0 41.0 33.0 41.0 32-33 37.192475460346586 40.0 35.5 41.0 33.0 41.0 34-35 36.90381414691879 40.0 35.0 41.0 32.5 41.0 36-37 36.62182386975916 39.0 35.0 41.0 31.5 41.0 38-39 36.28453976790547 39.0 35.0 41.0 30.5 41.0 40-41 35.94402569096709 39.0 35.0 41.0 30.0 41.0 42-43 35.54227875672434 39.0 35.0 41.0 28.0 41.0 44-45 34.9584296339123 39.0 35.0 41.0 24.0 41.0 46-47 34.14748836164272 39.0 35.0 41.0 11.5 41.0 48-49 33.42700070266627 39.0 34.0 40.5 2.0 41.0 50-51 32.59060888679632 38.5 33.5 39.5 2.0 40.5 52-53 32.597693508708915 38.0 33.0 40.0 2.0 40.5 54-55 32.65897645424011 38.5 33.5 40.0 2.0 41.0 56-57 32.364936380796465 38.0 33.0 40.0 2.0 41.0 58-59 32.080433105741974 38.0 33.0 40.0 2.0 41.0 60-61 31.729907823351702 38.0 32.0 40.0 2.0 41.0 62-63 31.27343091473982 37.5 31.0 40.0 2.0 41.0 64-65 30.842177721615357 37.0 31.0 40.0 2.0 41.0 66-67 30.389677102667985 36.0 30.0 39.0 2.0 41.0 68-69 29.863498133140233 35.5 29.0 39.0 2.0 40.0 70-71 29.418528636870374 35.0 28.5 38.5 2.0 40.0 72-73 28.861196221344166 35.0 27.5 38.0 2.0 40.0 74-75 28.263725963017716 35.0 26.0 37.0 2.0 39.0 76-77 27.649229285298247 34.0 25.5 36.5 2.0 39.0 78-79 26.99574321626522 34.0 24.0 36.0 2.0 38.5 80-81 26.382092314033144 33.5 21.0 35.5 2.0 37.0 82-83 25.727647413229302 33.0 18.5 35.0 2.0 37.0 84-85 25.13186512814715 32.5 10.5 35.0 2.0 36.0 86-87 24.51171062743375 32.0 4.5 35.0 2.0 36.0 88-89 23.796477938998265 31.5 2.0 35.0 2.0 35.0 90-91 23.091623531162746 31.0 2.0 34.0 2.0 35.0 92-93 22.316101075179567 30.5 2.0 34.0 2.0 35.0 94-95 21.509111764007173 29.5 2.0 34.0 2.0 35.0 96-97 20.48729118576006 29.0 2.0 34.0 2.0 35.0 98-99 19.237621410284113 28.0 2.0 34.0 2.0 35.0 100-101 15.799342270027061 13.0 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.33169069085192504 1101 2 0.2030025837503402 1101 3 0.10766017649009996 1101 4 0.1419199761350569 1101 5 0.21404800884656794 1101 6 0.2627096756009095 1101 7 0.3006372419414376 1101 8 0.34379538191815584 1101 9 0.3786075466501515 1101 10-11 0.36737129300799864 1101 12-13 0.4024601882089982 1101 14-15 0.5421472165119141 1101 16-17 0.644011212198933 1101 18-19 0.35265440168772955 1101 20-21 0.38205718402007705 1101 22-23 0.3386617372031182 1101 24-25 0.3410927189763484 1101 26-27 0.5363542000351984 1101 28-29 0.7282662321148194 1101 30-31 0.638750991939979 1101 32-33 0.6807650655142083 1101 34-35 0.5466785886162882 1101 36-37 0.3618136973889037 1101 38-39 0.45365958553575325 1101 40-41 0.39408601725515524 1101 42-43 0.3479625132382438 1101 44-45 0.49180968429114813 1101 46-47 0.49945694036738786 1101 48-49 0.5437283949462994 1101 50-51 0.2067584571742671 1101 52-53 0.26373878363910563 1101 54-55 0.30951266372893826 1101 56-57 0.4661601124731689 1101 58-59 0.6440637853335005 1101 60-61 0.6313510971080163 1101 62-63 0.36218931930875087 1101 64-65 0.2131384498348794 1101 66-67 0.13766819536867203 1101 68-69 0.6074997957300816 1101 70-71 0.8926965900067962 1101 72-73 0.8716754082374791 1101 74-75 0.9283746953524492 1101 76-77 0.7478812777749937 1101 78-79 0.9546177220054624 1101 80-81 1.1144849233973666 1101 82-83 1.2876689427814902 1101 84-85 1.8275471405348327 1101 86-87 1.7767634339038842 1101 88-89 2.0686870028949933 1101 90-91 2.0245590774253728 1101 92-93 2.3644872663350647 1101 94-95 2.4175307065270495 1101 96-97 2.872686797554831 1101 98-99 3.1339989529845056 1101 100-101 3.23848834465454 1102 1 0.38336187423042745 1102 2 0.24627661069351348 1102 3 0.09843822919815892 1102 4 0.10012271758275659 1102 5 0.13084624929273758 1102 6 0.10095640589429422 1102 7 0.09218509944899722 1102 8 0.18984227716971702 1102 9 0.19393294628095958 1102 10-11 0.21709439893471227 1102 12-13 0.2170707690974183 1102 14-15 0.41760772474018637 1102 16-17 0.3044173235064207 1102 18-19 0.20616761797931815 1102 20-21 0.25577384872295994 1102 22-23 0.39347498098772604 1102 24-25 0.1800817610406824 1102 26-27 0.2350423898156535 1102 28-29 0.2066174359167121 1102 30-31 0.36937037059752953 1102 32-33 0.3530560203078039 1102 34-35 0.7166365635583603 1102 36-37 0.7497560405804506 1102 38-39 0.5750609444768813 1102 40-41 0.5690987818755531 1102 42-43 0.6037380130222303 1102 44-45 0.6785973202834512 1102 46-47 0.9034944879892421 1102 48-49 0.8876151236686596 1102 50-51 0.7995552836932589 1102 52-53 0.6802346007711932 1102 54-55 0.7401909560191058 1102 56-57 0.7747575752593931 1102 58-59 0.7250975232071113 1102 60-61 0.7569667445688424 1102 62-63 0.7459723600377082 1102 64-65 0.9429683858153943 1102 66-67 0.8209327961306201 1102 68-69 1.1125624405594259 1102 70-71 1.0112072533742982 1102 72-73 1.1045924185670017 1102 74-75 0.8864556819594149 1102 76-77 1.1015476695113726 1102 78-79 1.364962955892583 1102 80-81 1.4894623398381945 1102 82-83 1.3615034347495971 1102 84-85 1.4230697044561254 1102 86-87 1.7507000428351915 1102 88-89 1.86732413419384 1102 90-91 2.03411094105995 1102 92-93 2.1445587482093025 1102 94-95 2.436673709551279 1102 96-97 2.482611780862637 1102 98-99 2.4530673320740917 1102 100-101 3.131756480234449 1103 1 0.5456838115523617 1103 2 0.39276180826566787 1103 3 0.38139375997977965 1103 4 0.22167294674168403 1103 5 0.4366402539997907 1103 6 0.4302206199411174 1103 7 0.43545587858933743 1103 8 0.41221314301884604 1103 9 0.5077351263874874 1103 10-11 0.526853782063661 1103 12-13 0.690616848621751 1103 14-15 0.8170534078815237 1103 16-17 0.8502103991908001 1103 18-19 0.780695364789679 1103 20-21 0.7921806987167628 1103 22-23 0.8051016996796605 1103 24-25 0.884471393147706 1103 26-27 0.7989792782090603 1103 28-29 0.7414619794569077 1103 30-31 0.6796515547917679 1103 32-33 0.7138404251139576 1103 34-35 0.7068802771716349 1103 36-37 0.6442949356628205 1103 38-39 0.3536361333781599 1103 40-41 0.44925330931268803 1103 42-43 0.5886754588292362 1103 44-45 0.5746505223149114 1103 46-47 0.5663815807676329 1103 48-49 0.6661330228387286 1103 50-51 0.4445633352230445 1103 52-53 0.4874020106559911 1103 54-55 0.6206133516588963 1103 56-57 0.6928564852061285 1103 58-59 0.5307351424316806 1103 60-61 0.7832116349441662 1103 62-63 0.80552890655078 1103 64-65 1.0290624648442588 1103 66-67 1.1683748870509731 1103 68-69 1.122188663772608 1103 70-71 1.3293835818767157 1103 72-73 1.4676885414457317 1103 74-75 1.3721286040453826 1103 76-77 1.3767117968494134 1103 78-79 1.6291955831686877 1103 80-81 1.6070193198951763 1103 82-83 1.5266542460960615 1103 84-85 1.5186196858756702 1103 86-87 1.449074253165925 1103 88-89 1.6692967589925516 1103 90-91 2.114779527706798 1103 92-93 2.405731794164957 1103 94-95 2.600754596458259 1103 96-97 2.9502430827330706 1103 98-99 3.3566146502953274 1103 100-101 3.488214736149226 1104 1 0.3635069677775782 1104 2 0.33895813770941885 1104 3 0.29555485661510517 1104 4 0.3379950267235614 1104 5 0.3562250506464437 1104 6 0.3145697759397592 1104 7 0.2847506559769002 1104 8 0.31072906009146095 1104 9 0.38751591261156904 1104 10-11 0.5100799630991162 1104 12-13 0.4628143675976233 1104 14-15 0.635250417057911 1104 16-17 0.6001183520626654 1104 18-19 0.562774496997676 1104 20-21 0.5342405718333438 1104 22-23 0.38591199762913675 1104 24-25 0.42744040866826793 1104 26-27 0.5750541054436766 1104 28-29 0.5752446915901359 1104 30-31 0.5149749942389121 1104 32-33 0.5530114345169181 1104 34-35 0.6291061183407791 1104 36-37 0.5113916276306512 1104 38-39 0.30613273471496427 1104 40-41 0.17874662527507468 1104 42-43 0.22732958820501636 1104 44-45 0.2988022726264532 1104 46-47 0.2532783180509668 1104 48-49 0.5732900410782236 1104 50-51 0.8290257799947724 1104 52-53 0.546313588768605 1104 54-55 0.8536653512057413 1104 56-57 0.595491865289965 1104 58-59 0.4785274274662328 1104 60-61 0.3050055626659258 1104 62-63 0.1847404166901221 1104 64-65 0.32540182136402507 1104 66-67 0.38672766829035155 1104 68-69 0.19910749236668934 1104 70-71 0.5640485303303251 1104 72-73 0.6554391928561785 1104 74-75 0.9006306432432929 1104 76-77 0.7557647027064434 1104 78-79 0.9901506075257736 1104 80-81 0.7377304901682002 1104 82-83 0.8157686111124853 1104 84-85 1.1258899928882435 1104 86-87 1.0082648048822485 1104 88-89 1.432956552806985 1104 90-91 1.5151406356709956 1104 92-93 1.5805307065927359 1104 94-95 1.8679036844836325 1104 96-97 2.0115818728089714 1104 98-99 2.302642462689114 1104 100-101 2.598834142516056 1105 1 0.17051611708086867 1105 2 0.17905143284642833 1105 3 0.12646570631754628 1105 4 0.07680385661942779 1105 5 0.11040147871037931 1105 6 0.13728001624102149 1105 7 0.14004583855904684 1105 8 0.23454355522267178 1105 9 0.34917459409277996 1105 10-11 0.17869165937622 1105 12-13 0.12886065474530994 1105 14-15 0.29893429071177735 1105 16-17 0.4369720025663284 1105 18-19 0.29148325833072875 1105 20-21 0.38170385137282636 1105 22-23 0.2523387980243612 1105 24-25 0.3364445079372729 1105 26-27 0.2925649646744901 1105 28-29 0.19928952462622362 1105 30-31 0.36861617666777846 1105 32-33 0.3106988585230184 1105 34-35 0.4210842693819572 1105 36-37 0.31623721630763413 1105 38-39 0.3717241459281979 1105 40-41 0.39609280947497183 1105 42-43 0.3822597929199034 1105 44-45 0.42700230204011547 1105 46-47 0.4115765932094817 1105 48-49 0.6912087558258548 1105 50-51 0.8896406910598955 1105 52-53 0.9835734376818479 1105 54-55 1.0974444670976027 1105 56-57 0.9285010270215608 1105 58-59 0.7898460912578287 1105 60-61 0.8776479651399853 1105 62-63 0.897728408876862 1105 64-65 0.9340811276160821 1105 66-67 1.0510536709955787 1105 68-69 0.9954075862003897 1105 70-71 0.9728962401045642 1105 72-73 0.9395564414781887 1105 74-75 0.9761843759808961 1105 76-77 1.3091038773427606 1105 78-79 1.1539007654688085 1105 80-81 1.2913845151780912 1105 82-83 1.2913532074722376 1105 84-85 1.4711648551929883 1105 86-87 1.5489633584347757 1105 88-89 1.6407805845903027 1105 90-91 1.8408844178939674 1105 92-93 1.7980881719643698 1105 94-95 1.6885642902806097 1105 96-97 1.7977425417831618 1105 98-99 1.8812048807399862 1105 100-101 2.153414076190339 1106 1 0.06700812435866865 1106 2 0.01889114983821827 1106 3 -0.007014507652861823 1106 4 0.12199789879793599 1106 5 0.1197480741574708 1106 6 0.13251126558356674 1106 7 0.23138066017894232 1106 8 0.046306473511066315 1106 9 0.04825824592722938 1106 10-11 0.21422060499142503 1106 12-13 0.21050018550648275 1106 14-15 0.19086014131429607 1106 16-17 0.20010652107445992 1106 18-19 0.21204791113529353 1106 20-21 0.14645035565262532 1106 22-23 0.0035222196029494057 1106 24-25 -0.0405312703937426 1106 26-27 0.13636208238549585 1106 28-29 0.095649066607983 1106 30-31 -0.030001119403848975 1106 32-33 -0.03213496376309877 1106 34-35 0.07615632660697003 1106 36-37 0.14219340146295423 1106 38-39 0.04617022670864657 1106 40-41 0.057768651311413066 1106 42-43 0.11320134845207974 1106 44-45 -0.02839390555638488 1106 46-47 0.23631818848124908 1106 48-49 0.3850814566763603 1106 50-51 0.03722261500143986 1106 52-53 -0.16218514042007826 1106 54-55 -0.24917143419236965 1106 56-57 -0.22027211057481821 1106 58-59 -0.07259162083275328 1106 60-61 -0.24462237315299973 1106 62-63 -0.36063346293569154 1106 64-65 -0.2513098982497155 1106 66-67 -0.027640454947746917 1106 68-69 -0.1630686343309513 1106 70-71 0.3069805347853034 1106 72-73 0.34793039100890866 1106 74-75 0.2686580095429285 1106 76-77 0.37705273015433605 1106 78-79 0.37687297502890615 1106 80-81 0.39925509658386105 1106 82-83 0.44754440164688347 1106 84-85 0.864640354957281 1106 86-87 0.6154443788850799 1106 88-89 0.6532995290756567 1106 90-91 0.8049590504001713 1106 92-93 0.8885892414268355 1106 94-95 0.9971694696736826 1106 96-97 0.945675109155399 1106 98-99 1.500373972690383 1106 100-101 1.9200413928720028 1107 1 0.25855767029934285 1107 2 0.16231002948509854 1107 3 0.07087849080148345 1107 4 0.06351626713207281 1107 5 0.13836393959113735 1107 6 0.1333426684246959 1107 7 0.16990597828500853 1107 8 0.0806358526420965 1107 9 0.14518659033963388 1107 10-11 0.07542262454450821 1107 12-13 0.13007140838669073 1107 14-15 0.26952218739668865 1107 16-17 0.1872670158347276 1107 18-19 0.2932504597629162 1107 20-21 0.34117718313276413 1107 22-23 0.30514761215736286 1107 24-25 0.2561538886876349 1107 26-27 0.41254182412153995 1107 28-29 0.4794302867294462 1107 30-31 0.6461013396590047 1107 32-33 0.3119921199402711 1107 34-35 0.2947869305455484 1107 36-37 0.356047218168861 1107 38-39 0.45753607041247335 1107 40-41 0.26370524550745955 1107 42-43 -0.0031704851313065774 1107 44-45 0.06671291081318742 1107 46-47 0.3023362979467734 1107 48-49 0.18227584999792867 1107 50-51 0.006432366600947148 1107 52-53 -0.40179543923381544 1107 54-55 -0.4619189182540069 1107 56-57 -0.38572599983952216 1107 58-59 -0.24475152983193027 1107 60-61 -0.23089005463501167 1107 62-63 -0.14057246819998426 1107 64-65 -0.026634617745731504 1107 66-67 0.03927424689985415 1107 68-69 0.028368483270977407 1107 70-71 0.18356161734738308 1107 72-73 0.47597115242275123 1107 74-75 0.4861870047170136 1107 76-77 0.4395515491843902 1107 78-79 0.4478596612461381 1107 80-81 0.49365616531442313 1107 82-83 0.5997298573879064 1107 84-85 0.7197032203493805 1107 86-87 1.0742001845333284 1107 88-89 1.2275164042858684 1107 90-91 1.0859373092158755 1107 92-93 1.4777383006189382 1107 94-95 1.8319829520044841 1107 96-97 2.0993996049284753 1107 98-99 2.5941382624267106 1107 100-101 2.737329281222161 1108 1 0.1255742853170041 1108 2 -0.01372061659424162 1108 3 -0.019879800896433153 1108 4 0.02716199411814557 1108 5 0.015027925930503727 1108 6 -0.15115992004869128 1108 7 -0.12515288350948595 1108 8 -0.22589179839735607 1108 9 -5.55754916639728E-4 1108 10-11 0.0013507255175611022 1108 12-13 -0.021106279243760184 1108 14-15 -0.08863940923513525 1108 16-17 -0.05119688557741142 1108 18-19 0.06842460574739562 1108 20-21 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2316 68-69 -1.0170671890702039 2316 70-71 -0.9687509657143529 2316 72-73 -0.9463063062447681 2316 74-75 -1.1794362969760428 2316 76-77 -1.114646785813516 2316 78-79 -1.9706552710299938 2316 80-81 -2.100127249751395 2316 82-83 -1.7728700691891248 2316 84-85 -1.759525934661255 2316 86-87 -1.4805311883960393 2316 88-89 -1.728206178727774 2316 90-91 -1.9678257782680717 2316 92-93 -1.9580438543933028 2316 94-95 -2.1657983277033637 2316 96-97 -2.6321769208825856 2316 98-99 -2.6796741050151702 2316 100-101 -2.774084142997479 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4663.0 3 686.0 4 595.0 5 594.0 6 637.0 7 704.0 8 903.0 9 1071.0 10 1565.0 11 2324.0 12 3825.0 13 5869.0 14 6836.0 15 9903.0 16 13917.0 17 19324.0 18 17566.0 19 9392.0 20 7681.0 21 6786.0 22 6450.0 23 7125.0 24 7435.0 25 8161.0 26 9330.0 27 10613.0 28 12184.0 29 14455.0 30 17531.0 31 20895.0 32 26022.0 33 32827.0 34 43750.0 35 62508.0 36 87060.0 37 125888.0 38 86243.0 39 5449.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.582548139543576 19.702062445549196 15.377626218044746 19.337763196862483 2 15.888211722565101 22.026660023851143 40.92117526295671 21.163952990627045 3 18.9526013149756 28.129080662527777 31.63129137934984 21.287026643146778 4 11.322005552683917 19.342755809341625 38.181359992325326 31.15387864564913 5 11.111381632936574 39.751718251200515 34.208630569189054 14.928269546673848 6 27.45519826192314 38.278650842185314 18.364809958710648 15.901340937180898 7 24.323945113849817 37.567863196957894 20.796920098960005 17.31127159023228 8 25.912261411857678 33.79676556656989 20.113989726321577 20.176983295250857 9 27.387708818931007 15.945261807540104 21.122425019901154 35.54460435362773 10-11 23.85957376744053 28.20701701637208 26.733243132959643 21.200166083227742 12-13 26.290478566412517 27.439058000219053 27.266967943573935 19.0034954897945 14-15 22.95548413419898 27.45102109475101 29.07238928297889 20.52110548807112 16-17 18.655188136657213 30.396487438110352 29.91282301260591 21.035501412626527 18-19 17.873576877262106 27.199397535162873 35.76934530627205 19.157680281302973 20-21 20.915349926726474 25.658620539066924 35.821626393073984 17.604403141132615 22-23 19.565671808469514 26.9013312181978 35.251797531132866 18.281199442199817 24-25 18.56525583609879 26.780793229731614 36.241269568517104 18.412681365652485 26-27 18.473789488304032 27.152732734538652 35.81750078393134 18.555976993225975 28-29 18.076817903943258 27.453710398105606 35.853485338941674 18.615986359009455 30-31 18.9448403480452 27.15325227550118 35.93756375519437 17.96434362125925 32-33 18.039650809444897 27.270221805439725 36.07407592997577 18.61605145513961 34-35 18.487388340604287 27.667118625624788 35.70552129671655 18.139971737054385 36-37 18.675780749470817 27.401806352226515 35.14448169201817 18.777931206284507 38-39 18.22991482170962 27.794203639169275 35.25641163280219 18.71946990631891 40-41 19.13721224006327 27.332014042219697 35.00155666808616 18.529217049630873 42-43 18.54176567250491 27.43969470893715 34.80136327460737 19.217176343950577 44-45 19.29410114510509 27.837053141721512 33.98073051707567 18.888115196097726 46-47 19.176455599804175 27.801190418249643 33.17274520525983 19.849608776686352 48-49 19.169141334867877 27.864585010847026 33.01869439153939 19.947579262745705 50-51 19.533682093707284 28.590740913026984 31.704822769138286 20.170754224127442 52-53 19.437639776000573 28.840749288819712 31.466695292791584 20.254915642388134 54-55 19.81422863518377 28.525329758843604 31.31390408277625 20.346537523196375 56-57 19.869280484462298 28.969408370027978 30.738766358725094 20.422544786784634 58-59 19.896232525020487 28.776223651112282 31.026117199995706 20.301426623871528 60-61 19.92386876629901 28.528100855635486 31.0757562311962 20.472274146869303 62-63 19.609637892018853 28.247614358363666 31.352634117645373 20.79011363197211 64-65 19.558555579887816 28.450850984955505 30.99385825171292 20.99673518344376 66-67 19.577966013154214 28.595564836130507 30.752893003764786 21.073576146950494 68-69 19.99343048768968 28.32690694765287 30.64462802820167 21.035034536455786 70-71 19.829969533643606 28.163881372295723 30.69938072496617 21.306768369094502 72-73 19.824278154927782 27.96413425108887 30.94705443698732 21.264533156996027 74-75 20.082355828993574 28.037908871858253 30.397301751671556 21.482433547476617 76-77 19.63496493487906 28.22620581079147 30.689494776012594 21.449334478316874 78-79 19.826344957090758 28.142622415856096 30.248295366718004 21.782737260335143 80-81 19.872754598153584 28.25899949903385 29.99176984183783 21.87647606097474 82-83 20.14387305049655 28.45493196731225 29.95220214363975 21.44899283855145 84-85 20.332366519175622 28.038039950678883 29.807216827575743 21.822376702569752 86-87 20.162964998375646 28.1343248337362 29.770629662864103 21.93208050502405 88-89 20.79842939431765 27.80223995968989 29.66772786093011 21.731602785062353 90-91 20.870472047279883 28.00504177501401 29.412000483850072 21.712485693856035 92-93 20.880021355364285 27.733561772752875 29.74264066522246 21.643776206660384 94-95 21.015600619558462 27.9662363541103 29.23011844331641 21.78804458301482 96-97 21.31619216394142 27.719581271444955 29.032151859958116 21.932074704655513 98-99 21.559786346716365 27.42093747785777 28.70155459030344 22.317721585122424 100-101 22.37196726868045 26.61960052540948 28.935859483777303 22.072572722132765 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3188.0 1 1822.5 2 544.0 3 637.5 4 471.0 5 372.5 6 675.5 7 1347.0 8 1927.0 9 2082.5 10 1765.5 11 1377.0 12 1438.0 13 1660.0 14 1839.0 15 1973.0 16 2138.5 17 2359.5 18 2664.0 19 2977.5 20 3319.0 21 3762.5 22 4244.0 23 4763.5 24 5378.0 25 6226.5 26 7415.5 27 8813.5 28 9950.5 29 11172.0 30 12481.5 31 13776.5 32 15203.0 33 16637.0 34 18707.5 35 20874.0 36 22832.0 37 24311.0 38 24024.5 39 23149.5 40 22973.5 41 22962.5 42 22865.5 43 23129.0 44 23547.0 45 23664.0 46 23841.0 47 23958.0 48 24337.5 49 24856.0 50 24949.0 51 23530.0 52 20763.5 53 18350.5 54 16411.0 55 14519.5 56 12510.0 57 10398.5 58 8383.0 59 6517.5 60 4789.5 61 3414.0 62 2402.5 63 1705.5 64 1261.0 65 914.0 66 663.5 67 527.5 68 405.0 69 305.0 70 239.5 71 214.0 72 175.5 73 137.5 74 117.0 75 87.5 76 69.5 77 45.0 78 28.5 79 24.5 80 17.5 81 15.0 82 12.0 83 5.0 84 4.5 85 5.0 86 2.5 87 2.0 88 1.5 89 1.0 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4558028641879196 2 0.038353270832766864 3 0.050231336053362566 4 0.05237797434624131 5 0.07556166790933172 6 0.07498923103123073 7 0.04336209351615059 8 0.040499909125645606 9 0.045079404150453584 10-11 0.04608116868713033 12-13 0.04228877436971122 14-15 0.03606352332036287 16-17 0.035133313393448745 18-19 0.04336209351615059 20-21 0.05101843676075144 22-23 0.04422074883330209 24-25 0.06003431759084216 26-27 0.05209175590719081 28-29 0.04064301834517085 30-31 0.04214566515018597 32-33 0.04214566515018597 34-35 0.04665360556523133 36-37 0.04114390061350923 38-39 0.02482944958763078 40-41 0.02282592051427729 42-43 0.02218192902641367 44-45 0.013953148903711823 46-47 0.027047642490272153 48-49 0.02697608788050953 50-51 0.014597140391575447 52-53 0.014954913440388571 54-55 0.016099787196590568 56-57 0.013666930464661324 58-59 0.012808275147509828 60-61 0.015026468050151197 62-63 0.015455795708726943 64-65 0.015169577269676443 66-67 0.007942561683651346 68-69 0.012164283659646205 70-71 0.01824642548946931 72-73 0.015169577269676443 74-75 0.013094493586560327 76-77 0.009588317708191716 78-79 0.011878065220595705 80-81 0.01674377868445419 82-83 0.013666930464661324 84-85 0.011520292171782583 86-87 0.004007058146706985 88-89 0.026188987173120654 90-91 0.02912272617338827 92-93 0.016242896416115815 94-95 0.029337390002676142 96-97 0.025187222636443907 98-99 0.022682811294752043 100-101 0.14675850462314333 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 698767.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.62107463258489 #Duplication Level Percentage of deduplicated Percentage of total 1 78.79890031903084 49.34471817843647 2 12.680190779251385 15.880943462858315 3 4.264658910657611 8.011725717804255 4 1.8254979335267325 4.57258569348028 5 0.8740626047110586 2.736736980158137 6 0.49283129040594775 1.8516975010670382 7 0.28342280466999026 1.242376842267119 8 0.17089093281699222 0.8561099086371936 9 0.10783147514455736 0.6077270567492145 >10 0.4394815341461457 4.735422289206287 >50 0.03340374796486243 1.489744861648185 >100 0.025167011461955067 2.9000995001467897 >500 0.002287910132486398 1.0329867302569518 >1k 6.863730397459193E-4 0.9859937139567742 >5k 4.5758202649727963E-4 2.1782982311826204 >10k+ 2.2879101324863981E-4 1.5728333321443573 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10978 1.5710530119481887 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9389 1.3436524621225674 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5815 0.8321801115393258 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3342 0.4782710116533837 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2067 0.29580675675869067 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1473 0.21079988036069247 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 974 0.139388379817593 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 847 0.12121350893788631 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 823 0.11777888766928031 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 757 0.10833367918061385 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 755 0.10804746074156335 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 714 0.10217998274102813 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 5.724368781009979E-4 0.0 22-23 0.0 0.0 0.0 0.0026475205612171153 0.0 24-25 0.0 0.0 0.0 0.006654578707924101 0.0 26-27 0.0 0.0 0.0 0.017316215562555186 0.0 28-29 0.0 0.0 0.0 0.0399274722475446 0.0 30-31 0.0 0.0 0.0 0.09989023522862414 0.0 32-33 0.0 0.0 0.0 0.2077230321408996 0.0 34-35 0.0 0.0 0.0 0.3610645608622044 0.0 36-37 0.0 0.0 0.0 0.5880357830292501 0.0 38-39 0.0 0.0 0.0 0.8898531270080012 0.0 40-41 0.0 0.0 0.0 1.2253011375751859 0.0 42-43 0.0 0.0 0.0 1.5213225581631646 0.0 44-45 0.0 0.0 0.0 1.8574861148279755 0.0 46-47 0.0 0.0 0.0 2.2142573991044223 0.0 48-49 0.0 0.0 0.0 2.592709730138945 0.0 50-51 0.0 0.0 0.0 2.9775304214423404 0.0 52-53 0.0 0.0 0.0 3.334015487279737 0.0 54-55 0.0 0.0 0.0 3.7006613077034265 0.0 56-57 0.0 0.0 0.0 4.104801743642731 0.0 58-59 0.0 0.0 0.0 4.548941206439342 0.0 60-61 0.0 0.0 0.0 4.998017937309575 0.0 62-63 0.0 0.0 0.0 5.438508115008293 0.0 64-65 0.0 0.0 0.0 5.883291569292769 0.0 66-67 0.0 0.0 0.0 6.364424765336658 0.0 68-69 0.0 0.0 0.0 6.892068457726252 0.0 70-71 0.0 0.0 0.0 7.453485925923806 0.0 72-73 0.0 0.0 0.0 8.009679907608687 0.0 74-75 0.0 0.0 0.0 8.54462217019407 0.0 76-77 0.0 0.0 0.0 9.089081195877881 0.0 78-79 0.0 0.0 0.0 9.69013991788393 0.0 80-81 0.0 0.0 0.0 10.313237459696866 0.0 82-83 0.0 0.0 0.0 10.951576133389242 0.0 84-85 0.0 0.0 0.0 11.585693085105621 0.0 86-87 0.0 0.0 0.0 12.221956675114882 0.0 88-89 0.0 1.4310921952524947E-4 0.0 12.891636267883285 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5215 0.0 47.812454 1 GTATCAA 8715 0.0 38.676365 1 CATTCCG 150 0.0 38.00103 9 TAACGCA 60 0.003941079 31.681131 4 TATCAAC 10660 0.0 31.651411 2 ATCAACG 10940 0.0 30.797882 3 TCAACGC 11000 0.0 30.586693 4 CAACGCA 11160 0.0 30.233337 5 AACGCAG 11310 0.0 29.786083 6 GTGTAGC 650 0.0 27.13826 1 CGCAGAG 12565 0.0 26.538723 8 ACGCAGA 12670 0.0 26.468752 7 GCAGAGT 12730 0.0 25.635023 9 GACGTGG 260 0.0 25.577618 7 ACGAAGG 95 1.1794327E-8 25.023964 94-95 GAGTACT 7875 0.0 24.881628 12-13 GTGTCGA 50 0.0016556302 23.745546 86-87 GTACTTT 8425 0.0 23.144543 14-15 AGTACTT 7930 0.0 23.061785 12-13 CATGGGT 1390 0.0 22.906141 4 >>END_MODULE