FastQCFastQC Report
Thu 26 May 2016
SRR938018_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938018_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences698767
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104671.4979242007707863No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93801.34236447914684No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58750.8407666647108406No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30100.43075875077100095No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT22370.32013532407798306No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21670.3101176787112156No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15990.22883164202087392No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT14190.20307198250632902RNA PCR Primer, Index 26 (100% over 22bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9160.13108804508512853No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8350.11949619830358331No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT7820.11191140966874509RNA PCR Primer, Index 26 (100% over 24bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7540.10790435152203812No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7110.10175065508245239No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7050.10089199976530089No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55350.044.628911
AGCGTGC550.002579669534.5434538
GTATCAA86450.034.4534841
AGCGTCA702.0523481E-433.9290283
CATTCCG1805.456968E-1231.6648319
TGTATAG4100.030.1202035
GTGTAGC6750.029.5580731
TATCAAC106150.027.9251272
TAGCCCT9900.027.8266684
GTATAGC5100.027.0121041
ATCAACG109350.026.9756743
TCAACGC111550.026.6120824
CAACGCA113550.026.1433535
AACGCAG115150.025.7800946
GCGGTAT950.001225465225.0021271
GCGTCAG950.001226488224.9985494
ATGGGCG1401.778985E-523.7486235
GAGTACT78750.023.16056312-13
GTAGCCC6450.022.8297673
CGCAGAG129450.022.822168
Produced by FastQC (version 0.11.5)