##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938018_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 698767 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49072437593647 33.0 31.0 34.0 31.0 34.0 2 32.69923164660037 34.0 31.0 34.0 31.0 34.0 3 32.61297828890031 34.0 31.0 34.0 31.0 34.0 4 36.16419922520669 37.0 35.0 37.0 35.0 37.0 5 36.162038275991854 37.0 35.0 37.0 35.0 37.0 6 36.24715677758108 37.0 37.0 37.0 35.0 37.0 7 36.207880452282375 37.0 37.0 37.0 35.0 37.0 8 36.22148870796703 37.0 37.0 37.0 35.0 37.0 9 37.89670376534667 39.0 38.0 39.0 35.0 39.0 10-11 37.888869251123765 39.0 38.0 39.0 35.0 39.0 12-13 37.83712167288953 39.0 38.0 39.0 35.0 39.0 14-15 39.02883793882653 40.0 38.0 41.0 36.0 41.0 16-17 38.982050526141045 40.0 38.0 41.0 35.5 41.0 18-19 39.095201261650885 40.0 38.5 41.0 36.0 41.0 20-21 39.176012891278496 40.5 39.0 41.0 36.0 41.0 22-23 39.13579204513093 40.5 39.0 41.0 35.0 41.0 24-25 39.0752983469454 41.0 39.0 41.0 35.0 41.0 26-27 38.87672428720876 40.0 38.0 41.0 35.0 41.0 28-29 38.7678603883698 40.0 38.0 41.0 35.0 41.0 30-31 38.41470189634027 40.0 37.5 41.0 35.0 41.0 32-33 38.01150025688105 40.0 36.5 41.0 34.0 41.0 34-35 37.72488111201588 40.0 36.0 41.0 33.5 41.0 36-37 37.539968973921205 40.0 36.0 41.0 33.0 41.0 38-39 37.345405550061756 40.0 36.0 41.0 33.0 41.0 40-41 37.11384409967843 40.0 35.0 41.0 33.0 41.0 42-43 36.74116837229005 40.0 35.0 41.0 32.0 41.0 44-45 36.17591843919361 40.0 35.0 41.0 30.5 41.0 46-47 35.375752575608175 40.0 35.0 41.0 25.0 41.0 48-49 34.63395380720612 40.0 35.0 41.0 14.0 41.0 50-51 34.1576548405978 39.0 35.0 41.0 3.5 41.0 52-53 33.677885475415984 39.0 34.5 41.0 2.0 41.0 54-55 33.33790805805082 39.0 34.0 40.0 2.0 41.0 56-57 33.036772629503105 38.5 34.0 40.0 2.0 41.0 58-59 32.66193309071551 38.0 33.0 40.0 2.0 41.0 60-61 32.260125335054454 38.0 33.0 40.0 2.0 41.0 62-63 31.84297269332982 37.5 32.0 40.0 2.0 41.0 64-65 31.43390357587007 37.0 31.5 40.0 2.0 41.0 66-67 30.92234607530121 36.0 31.0 39.0 2.0 41.0 68-69 30.382369945919024 36.0 30.5 39.0 2.0 40.0 70-71 29.844629182545827 35.0 29.5 38.5 2.0 40.0 72-73 29.40300772646676 35.0 29.0 38.0 2.0 40.0 74-75 28.89326341970929 35.0 28.5 37.0 2.0 39.0 76-77 27.64292246199377 33.5 26.5 35.5 2.0 38.0 78-79 28.229847001933408 34.0 28.0 36.0 2.0 38.5 80-81 28.045709800262465 34.0 28.0 36.0 2.0 37.5 82-83 27.652527237262206 34.0 26.5 35.0 2.0 37.0 84-85 27.199569384358448 34.0 26.0 35.0 2.0 36.5 86-87 26.735399639650986 34.0 25.5 35.0 2.0 36.0 88-89 26.249649024639112 33.0 24.5 35.0 2.0 36.0 90-91 25.838215742872805 33.0 22.0 35.0 2.0 35.0 92-93 25.346196944045726 33.0 19.0 35.0 2.0 35.0 94-95 24.78726098971474 32.0 12.0 35.0 2.0 35.0 96-97 24.165761262337803 32.0 2.0 34.0 2.0 35.0 98-99 23.385595627727128 32.0 2.0 34.0 2.0 35.0 100-101 20.355718715966837 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.030327627843007576 1101 2 -0.02880886155735851 1101 3 0.21284882377703696 1101 4 0.11562625046404662 1101 5 -0.04497021035309956 1101 6 0.03898930458989014 1101 7 -0.12838792639156793 1101 8 0.04646253912006415 1101 9 0.06327641381273708 1101 10-11 -0.007310403039433311 1101 12-13 -0.016489670721107075 1101 14-15 0.1239361476188634 1101 16-17 0.15595837735235563 1101 18-19 0.1824707826416514 1101 20-21 0.14092550401785076 1101 22-23 0.12856867293652385 1101 24-25 0.17428467945831017 1101 26-27 0.1359841779701938 1101 28-29 0.11827861852435007 1101 30-31 0.07725223238080048 1101 32-33 0.2914175725047201 1101 34-35 0.1409940344832208 1101 36-37 0.04723060337334317 1101 38-39 0.041806090496528725 1101 40-41 -0.07381910811165682 1101 42-43 -0.17551926171132237 1101 44-45 -0.20693288932950793 1101 46-47 -0.16577014120946387 1101 48-49 -0.1153724256323514 1101 50-51 0.030460572264949803 1101 52-53 0.14959378862783268 1101 54-55 0.01075914909768727 1101 56-57 -0.010112863296498631 1101 58-59 -0.02627907014474573 1101 60-61 0.1950701810149198 1101 62-63 0.40326384342536414 1101 64-65 0.2706830174593975 1101 66-67 0.22002476182496622 1101 68-69 0.3596005611150339 1101 70-71 0.3146845277337995 1101 72-73 0.10666998072934675 1101 74-75 0.3736788060561018 1101 76-77 0.27714693677184954 1101 78-79 0.3362576854425363 1101 80-81 0.480649631361004 1101 82-83 0.5664922052622394 1101 84-85 0.48196337898595587 1101 86-87 0.33299836944197736 1101 88-89 0.6886506215777821 1101 90-91 0.6782013203169512 1101 92-93 0.994246886230556 1101 94-95 0.9328247099484948 1101 96-97 1.4669342375493883 1101 98-99 1.7513411689071567 1101 100-101 2.3497992928891094 1102 1 0.0797993689372305 1102 2 0.07553110937858065 1102 3 0.17400510200648966 1102 4 0.12718117760751824 1102 5 0.022953280476016857 1102 6 -0.057377688065543 1102 7 0.11699913617471225 1102 8 0.024279592652916904 1102 9 0.032727731514320624 1102 10-11 0.043481003425362985 1102 12-13 -0.03994907922823643 1102 14-15 0.023017094950070316 1102 16-17 0.16877012865827368 1102 18-19 0.07885741317461736 1102 20-21 0.041663888213285816 1102 22-23 0.07652436988480105 1102 24-25 0.053943765904243435 1102 26-27 0.19515703056828215 1102 28-29 -0.046648132192437686 1102 30-31 0.2171053410568149 1102 32-33 0.1908048707255361 1102 34-35 0.11627387423813929 1102 36-37 0.3738595653151364 1102 38-39 0.4427290707408247 1102 40-41 0.31265039426453 1102 42-43 0.21812592111731277 1102 44-45 0.5627933586576077 1102 46-47 0.6096668015657585 1102 48-49 0.6520915388623649 1102 50-51 0.85108034368006 1102 52-53 0.9407056852540379 1102 54-55 1.1558477159253897 1102 56-57 1.2562317421785352 1102 58-59 1.1837087543711746 1102 60-61 1.4453313647861776 1102 62-63 1.2520678966832932 1102 64-65 1.0168595227027097 1102 66-67 1.099593514034229 1102 68-69 1.0401433520501904 1102 70-71 0.8574244044079293 1102 72-73 0.773254168310352 1102 74-75 1.1198513837239048 1102 76-77 1.0339200407347704 1102 78-79 1.016050309455732 1102 80-81 1.1617344277162118 1102 82-83 1.061834100710179 1102 84-85 1.2225116685268205 1102 86-87 1.04572371408673 1102 88-89 1.216744569183927 1102 90-91 0.958272802191189 1102 92-93 1.2341078500575868 1102 94-95 0.9886473406385718 1102 96-97 1.2904957630197131 1102 98-99 1.202187561429053 1102 100-101 1.7846329600663609 1103 1 0.33975539519760645 1103 2 0.3857627456971713 1103 3 0.49572874873013717 1103 4 0.3288689021213287 1103 5 0.2145982699470892 1103 6 0.21051838526531697 1103 7 0.3287884338335729 1103 8 0.27168142592431366 1103 9 0.48546215490092237 1103 10-11 0.46370273016882635 1103 12-13 0.3714871898167331 1103 14-15 0.5855449656301204 1103 16-17 0.45793617597649217 1103 18-19 0.46746140177860696 1103 20-21 0.4349764733736947 1103 22-23 0.5224320250315841 1103 24-25 0.5779744855871414 1103 26-27 0.5283231398213459 1103 28-29 0.3804112013886396 1103 30-31 0.5193566726559737 1103 32-33 0.7199901178455619 1103 34-35 0.5500856542455708 1103 36-37 0.4876230361438232 1103 38-39 0.6398986483451878 1103 40-41 0.6770564391053568 1103 42-43 0.34015459695033456 1103 44-45 0.6440286491320322 1103 46-47 0.822722666795535 1103 48-49 0.6661863611093608 1103 50-51 0.324863323984772 1103 52-53 0.2940981663639164 1103 54-55 0.67229761063615 1103 56-57 0.6225794443958037 1103 58-59 0.6513710236638772 1103 60-61 0.693897573134997 1103 62-63 1.0099320735692032 1103 64-65 1.076874387065402 1103 66-67 0.8265444616373507 1103 68-69 0.8409717305959603 1103 70-71 0.9889027891471862 1103 72-73 0.8023000620301595 1103 74-75 0.5605534151868063 1103 76-77 0.41213755590881007 1103 78-79 0.7938327323468535 1103 80-81 0.832861951561128 1103 82-83 0.7491219738676165 1103 84-85 0.9755062182605059 1103 86-87 1.0574433350454804 1103 88-89 1.1123998398391954 1103 90-91 1.4505355917365854 1103 92-93 1.6919720269434997 1103 94-95 1.283864422268696 1103 96-97 1.607154687830814 1103 98-99 2.1104171538977745 1103 100-101 2.341622307273102 1104 1 0.09812460330908124 1104 2 0.06566531735231962 1104 3 0.2074966200340853 1104 4 0.02511054707431981 1104 5 -0.04606220359998048 1104 6 0.06746015483594903 1104 7 0.07992415378372897 1104 8 0.15995320569093963 1104 9 0.11822810029573105 1104 10-11 0.1403709073524695 1104 12-13 0.0843029822716943 1104 14-15 0.06651670008123745 1104 16-17 0.23455195710258891 1104 18-19 0.13583117633394437 1104 20-21 0.32819755641436643 1104 22-23 0.2082326934353418 1104 24-25 0.2840467638243709 1104 26-27 0.32934840321774317 1104 28-29 0.26878210883170794 1104 30-31 0.33555413498745423 1104 32-33 0.4156808961394276 1104 34-35 0.38314473434073903 1104 36-37 0.29380435482514855 1104 38-39 0.41890057425432303 1104 40-41 0.39840570839277234 1104 42-43 0.29012485864740967 1104 44-45 0.26950304586931395 1104 46-47 0.6044718454519327 1104 48-49 0.5513157935326092 1104 50-51 0.31487691863754463 1104 52-53 0.322384956892968 1104 54-55 0.3964734348119734 1104 56-57 0.49739818235887157 1104 58-59 0.31877642753836 1104 60-61 0.5419971539665269 1104 62-63 0.49356042974910963 1104 64-65 0.47196898319092284 1104 66-67 0.7167308216690707 1104 68-69 0.879358215245329 1104 70-71 0.7777806638497999 1104 72-73 0.7614863654174933 1104 74-75 0.7393839918266636 1104 76-77 0.6799918665466258 1104 78-79 0.6690210749321004 1104 80-81 0.6163968445032353 1104 82-83 0.5684235485815634 1104 84-85 0.8563745767061803 1104 86-87 0.9904740929473732 1104 88-89 1.0125499474635298 1104 90-91 0.8944902195829663 1104 92-93 1.2259685716359208 1104 94-95 1.3119642863221692 1104 96-97 1.7130386234827881 1104 98-99 2.0688274291894935 1104 100-101 2.5086184554548154 1105 1 0.11127167778983704 1105 2 0.18458347489765004 1105 3 0.22557565990616268 1105 4 0.011279915861457823 1105 5 0.06035722266806687 1105 6 0.07203791355699707 1105 7 0.004053499288509954 1105 8 0.08808032238650298 1105 9 0.17926557370434182 1105 10-11 0.11528230390029393 1105 12-13 0.09871049393876774 1105 14-15 0.09196468866377217 1105 16-17 0.15426718322522248 1105 18-19 0.16910588949904337 1105 20-21 0.12789679145224397 1105 22-23 0.19732789217427893 1105 24-25 0.11562459918662427 1105 26-27 0.07753335295560504 1105 28-29 0.08281842847940624 1105 30-31 0.15120509849173658 1105 32-33 -0.012644037731632807 1105 34-35 0.21607207640288806 1105 36-37 0.21232957081871717 1105 38-39 0.10379024198361009 1105 40-41 0.13006412059404937 1105 42-43 0.11106095852125719 1105 44-45 0.06099960012197414 1105 46-47 0.21923894938776556 1105 48-49 -0.05697305682853937 1105 50-51 -0.08040011862043883 1105 52-53 -0.06420676709925033 1105 54-55 0.024261258802326324 1105 56-57 0.14094198902417787 1105 58-59 0.2689938192930086 1105 60-61 0.2008298462065099 1105 62-63 0.2692512881099418 1105 64-65 0.2425673438975977 1105 66-67 0.24941311899968 1105 68-69 0.3910677492046375 1105 70-71 0.6967709055596032 1105 72-73 0.4641671506377527 1105 74-75 0.26322976485683114 1105 76-77 0.19106755699070987 1105 78-79 0.4472555928899631 1105 80-81 0.447961215869249 1105 82-83 0.24752887638844356 1105 84-85 0.37027252790023013 1105 86-87 0.5631517089500555 1105 88-89 0.7816765898121467 1105 90-91 0.8298050388857838 1105 92-93 1.1774515790369762 1105 94-95 1.144714525049178 1105 96-97 1.2262975527015243 1105 98-99 1.6213970846561843 1105 100-101 2.1718911593773953 1106 1 0.006146829248315555 1106 2 -0.026020761654201863 1106 3 0.12511826503100565 1106 4 0.09133960316543721 1106 5 -0.03197108867212961 1106 6 -0.03287070486892674 1106 7 -0.04523138291477835 1106 8 -0.08188239252869778 1106 9 -0.08835523269763002 1106 10-11 0.004678651754161933 1106 12-13 -0.10912755554870301 1106 14-15 0.08116876703646625 1106 16-17 0.0449940280966743 1106 18-19 -0.07844739856232508 1106 20-21 -0.19243935871664775 1106 22-23 -0.15944790252009255 1106 24-25 -0.1932805751416069 1106 26-27 -0.3453504165391905 1106 28-29 -0.36253574086578055 1106 30-31 -0.22288176757415812 1106 32-33 -0.035893645205796076 1106 34-35 -0.3305157734126354 1106 36-37 -0.13743822530940264 1106 38-39 -0.06077256409888321 1106 40-41 -0.05091074606404078 1106 42-43 0.06098615458937928 1106 44-45 0.25913359266495206 1106 46-47 0.3191002504307434 1106 48-49 0.3427711682689534 1106 50-51 0.7030649008013015 1106 52-53 0.5421404634020561 1106 54-55 0.34967881668771383 1106 56-57 0.6945962779777375 1106 58-59 0.6758356518334949 1106 60-61 0.5038432368895158 1106 62-63 0.5912792928319419 1106 64-65 0.3448266564027094 1106 66-67 0.4853075129904276 1106 68-69 0.10602873038287797 1106 70-71 0.1664704430998789 1106 72-73 0.3631380121686618 1106 74-75 0.3142211117561686 1106 76-77 0.50182432343491 1106 78-79 0.7730491184538231 1106 80-81 0.7136924437835859 1106 82-83 0.5862251061923551 1106 84-85 0.4800502199651717 1106 86-87 0.5068120757230865 1106 88-89 0.7108709670499387 1106 90-91 0.7290807653994911 1106 92-93 0.8634854511923784 1106 94-95 1.1492943178578692 1106 96-97 1.227251108031112 1106 98-99 1.5085873009252602 1106 100-101 1.8696267820227277 1107 1 0.11972968421009256 1107 2 2.553472293413961E-4 1107 3 0.07269642634583562 1107 4 0.13216148266493377 1107 5 0.002619048622712228 1107 6 0.031808657067180945 1107 7 0.13342952943646935 1107 8 0.03156441469543836 1107 9 0.04769061538995345 1107 10-11 0.15660334933138387 1107 12-13 0.09241016179699812 1107 14-15 0.09866412818652748 1107 16-17 0.1388641327400606 1107 18-19 0.07569941206299546 1107 20-21 0.06232325687668805 1107 22-23 0.13295647670542365 1107 24-25 0.2526517864581663 1107 26-27 0.1973912477025479 1107 28-29 0.26004704985123084 1107 30-31 0.12162606083941085 1107 32-33 0.026820582742196564 1107 34-35 -0.020998667299188867 1107 36-37 0.1726778687600543 1107 38-39 0.19570198552641926 1107 40-41 0.2892350517614588 1107 42-43 0.3159613447727807 1107 44-45 0.2937492389105998 1107 46-47 0.479402276408301 1107 48-49 -0.002088116800180728 1107 50-51 -0.16795334093284708 1107 52-53 -0.3642539692221973 1107 54-55 -0.1600457622256357 1107 56-57 -0.12921245401955872 1107 58-59 -0.25422636326024417 1107 60-61 0.03104995008956024 1107 62-63 0.00186853070006876 1107 64-65 -0.025287434741713355 1107 66-67 -0.1590022903827979 1107 68-69 0.1978940204479933 1107 70-71 0.12411897132789562 1107 72-73 0.017528574990596013 1107 74-75 -0.2659223430249682 1107 76-77 0.022899440681609207 1107 78-79 0.2548215559379976 1107 80-81 0.1235627071493326 1107 82-83 0.2537735637511318 1107 84-85 0.2761647343692246 1107 86-87 0.4296531681864195 1107 88-89 0.45733372587130816 1107 90-91 0.5120071522361158 1107 92-93 0.839979425411336 1107 94-95 0.6728362451252394 1107 96-97 0.6695130150424831 1107 98-99 0.8234471150689338 1107 100-101 1.1555613355027177 1108 1 -0.11733833888288814 1108 2 -0.02101259816426193 1108 3 0.053230022291010926 1108 4 0.011561209813642392 1108 5 -0.035860211968341105 1108 6 -0.09216882818348893 1108 7 -0.06129052694075199 1108 8 -0.1355651354613201 1108 9 -0.16760712681074352 1108 10-11 0.042536148406220775 1108 12-13 -0.06650313112061212 1108 14-15 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-2.2685687395755245 2316 94-95 -2.2759215761042597 2316 96-97 -2.9055916287198507 2316 98-99 -3.53396163124188 2316 100-101 -4.141684661903433 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 5.0 5 18.0 6 61.0 7 142.0 8 282.0 9 519.0 10 959.0 11 1805.0 12 3093.0 13 4567.0 14 6193.0 15 8312.0 16 12004.0 17 18397.0 18 18132.0 19 10469.0 20 7009.0 21 5966.0 22 5798.0 23 5919.0 24 6496.0 25 7281.0 26 7687.0 27 7753.0 28 9012.0 29 10925.0 30 12969.0 31 16003.0 32 19498.0 33 25517.0 34 35839.0 35 54882.0 36 83958.0 37 150537.0 38 131509.0 39 9249.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.87304065589818 20.014826115142817 15.499014693023568 19.61311853593544 2 15.740510151797496 22.03811682939885 40.81972938081621 21.401643637987444 3 19.063648060131698 27.929838722373212 31.85308265102693 21.153430566468163 4 11.214753988096176 19.744922127118194 37.64745616206832 31.3928677227173 5 10.961544791423677 40.14104965789447 34.27352127551481 14.623884275167045 6 27.398156178176958 38.33887166805483 18.15915485298369 16.103817300784527 7 24.287781191727714 37.49833635532302 20.506835611870624 17.707046841078643 8 26.023913619027855 33.98095210836261 19.980107761550737 20.0150265110588 9 26.935731080603404 15.831171191541674 20.985249732743533 36.247847995111385 10-11 23.780101235461892 28.208616033670737 26.650585960699345 21.360696770168026 12-13 26.159792892337503 27.13028806454798 27.307099505271427 19.402819537843087 14-15 22.845741141181538 27.358189496641945 28.780552029503397 21.015517332673124 16-17 18.69228226290022 30.485626825536983 29.666183434535405 21.15590747702739 18-19 17.994410153885344 27.16155742901425 35.38754692193535 19.456485495165055 20-21 20.759566493552214 25.68917822392872 35.44157065230613 18.109684630212932 22-23 19.50507107519388 27.097873826325515 34.88881129188986 18.50824380659075 24-25 18.502513770999972 27.02035489036867 35.97604869259579 18.50108264603557 26-27 18.39974006046171 27.258010031146938 35.731237403810916 18.611012504580433 28-29 17.761921219523888 27.42669382889371 35.92190215730765 18.889482794274752 30-31 18.89344930921081 27.138555682445915 35.609488727270644 18.358506281072636 32-33 18.16296607968194 27.332750856720057 35.95712571405264 18.54715734954536 34-35 18.464839271580146 27.858853266882605 35.59563051141967 18.08067695011758 36-37 18.48762046246423 27.567665430823933 34.94143164544552 19.003282461266313 38-39 18.075285539556862 27.751317393168236 35.25052835284881 18.92286871442609 40-41 19.05043074727513 27.270235316767614 35.000282703619966 18.679051232337287 42-43 18.563921187497808 27.35335731783859 34.89845708588977 19.184264408773835 44-45 19.104939663075005 27.812878604096312 34.03134242591436 19.050839306914327 46-47 19.124940518996354 27.713890111305506 33.092618631060354 20.068550738637786 48-49 19.134571901242765 27.7434870714576 32.96127161674042 20.160669410559215 50-51 19.30700791537093 28.41777015800689 31.85117499824691 20.424046928375265 52-53 19.27888695373422 28.7610176210382 31.640017344837407 20.32007808039017 54-55 19.55637573039368 28.465139309669745 31.286966900268613 20.691518059667956 56-57 19.720593559798903 29.059471898358108 30.541367866542068 20.67856667530092 58-59 19.465215157556095 29.097181177703014 30.915097593332252 20.52250607140864 60-61 19.74098662358125 29.103549537971883 30.554533914738393 20.600929923708474 62-63 19.117316644890213 29.13038251663287 30.764332030562404 20.987968807914513 64-65 19.021862795466873 29.15685772224504 30.527056944589543 21.294222537698545 66-67 18.923730493277073 29.25144415401161 30.287914901351026 21.536910451360292 68-69 19.38859404393313 28.773989594521204 30.225261228178514 21.612155133367157 70-71 19.24840468997534 28.56474332645932 30.188818304241614 21.998033679323722 72-73 19.193379195067884 28.10858268922259 30.50122573046523 22.196812385244293 74-75 19.241893220486944 28.24761329599137 29.82639420579392 22.684099277727768 76-77 18.617865468747095 28.123966930321554 30.68733927045782 22.57082833047353 78-79 18.744946455685515 28.034380558898746 30.298940848666295 22.921732136749444 80-81 18.603997186470732 28.052146174723603 30.182399986261505 23.161456652544164 82-83 18.617556036564643 28.20335951056332 30.20454016922774 22.9745442836443 84-85 18.82010733452594 27.79277280858676 29.967155635062614 23.41996422182469 86-87 18.426029482162367 27.78623321405086 30.13868108074071 23.64905622304606 88-89 18.875533618502306 27.55689664795275 29.868826089383155 23.698743644161787 90-91 18.663837825847402 27.462343739937463 30.032557906562385 23.841260527652754 92-93 18.388156247093086 27.266963857663164 30.32829348922742 24.01658640601633 94-95 18.228549880396383 27.436132098490695 30.00158135800729 24.33373666310563 96-97 18.418521847147247 27.23859557733369 29.849419832733226 24.49346274278583 98-99 18.52406534967457 26.958386522488283 29.685773737628157 24.831774390208995 100-101 18.374226565848613 26.73092899396852 29.916329219084638 24.97851522109823 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2937.0 1 1663.0 2 512.0 3 633.0 4 460.5 5 371.5 6 719.5 7 1405.5 8 2018.5 9 2159.5 10 1790.5 11 1434.5 12 1447.5 13 1629.0 14 1874.0 15 2008.0 16 2132.5 17 2398.5 18 2656.0 19 2907.5 20 3245.0 21 3614.0 22 4036.0 23 4528.5 24 5193.0 25 6013.0 26 7130.5 27 8478.5 28 9489.5 29 10673.0 30 12020.0 31 13390.0 32 14952.0 33 16530.0 34 18677.0 35 20974.0 36 23078.5 37 24315.0 38 23953.5 39 23204.5 40 22904.0 41 22805.0 42 23012.5 43 23346.0 44 23702.0 45 24155.5 46 24478.0 47 24820.5 48 25296.5 49 25387.5 50 25625.0 51 24348.5 52 21595.5 53 18972.5 54 16554.5 55 14599.0 56 12538.0 57 10403.5 58 8282.5 59 6330.5 60 4464.5 61 3065.5 62 2114.0 63 1423.5 64 944.5 65 656.0 66 470.5 67 342.5 68 256.5 69 176.5 70 111.0 71 85.0 72 90.0 73 85.5 74 62.0 75 34.5 76 19.5 77 11.5 78 6.0 79 2.5 80 1.0 81 0.5 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.010446973025343212 3 0.004865713463858482 4 0.0 5 8.586553171514967E-4 6 1.4310921952524947E-4 7 0.0 8 2.8621843905049895E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0022897475124039916 26-27 0.020464618392110674 28-29 3.577730488131237E-4 30-31 1.4310921952524947E-4 32-33 0.0037923943174191107 34-35 0.014883358830625946 36-37 0.007656343244600847 38-39 0.019105080806620805 40-41 0.022253483636176293 42-43 0.017387770172317813 44-45 0.009373653878903841 46-47 0.002075083683116117 48-49 4.2932765857574837E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.1466382928787419E-4 68-69 7.155460976262474E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 7.155460976262474E-5 82-83 6.439914878636227E-4 84-85 0.002432856731929241 86-87 7.871007073888721E-4 88-89 0.0 90-91 0.0017173106343029935 92-93 2.8621843905049895E-4 94-95 7.155460976262474E-5 96-97 4.2932765857574837E-4 98-99 4.2932765857574837E-4 100-101 0.0023613021221666163 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 698767.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.94288081988657 #Duplication Level Percentage of deduplicated Percentage of total 1 77.80573314783031 47.417055223321206 2 13.261663879927593 16.164080026156434 3 4.462583139815434 8.158880173158217 4 1.83753485601341 4.479386709296507 5 0.921953752542021 2.8093258831307795 6 0.5221761678667485 1.9093751979173006 7 0.31881203986166196 1.3600526904463937 8 0.19439936404939315 0.9477805819779127 9 0.12563287412905125 0.6890786347595924 >10 0.47886588454996265 4.922891470815194 >50 0.03956125209674566 1.6971529528622302 >100 0.02684496295650908 3.217198090880731 >500 0.0023548213117528063 1.0392964837620973 >1k 0.0011774106558764031 1.4970920903902512 >5k 4.7096426235056124E-4 2.1892388649255445 >10k+ 2.3548213117528062E-4 1.5021149261996511 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10467 1.4979242007707863 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9380 1.34236447914684 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5875 0.8407666647108406 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3010 0.43075875077100095 No Hit CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 2237 0.32013532407798306 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2167 0.3101176787112156 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1599 0.22883164202087392 No Hit ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT 1419 0.20307198250632902 RNA PCR Primer, Index 26 (100% over 22bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 916 0.13108804508512853 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 835 0.11949619830358331 No Hit ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT 782 0.11191140966874509 RNA PCR Primer, Index 26 (100% over 24bp) GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 754 0.10790435152203812 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 711 0.10175065508245239 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 705 0.10089199976530089 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 5.724368781009979E-4 0.0 22-23 0.0 0.0 0.0 0.0025759659514544902 0.0 24-25 0.0 0.0 0.0 0.0065114694883988515 0.0 26-27 0.0 0.0 0.0 0.017029997123504687 0.0 28-29 0.0 0.0 0.0 0.03999902685730723 0.0 30-31 0.0 0.0 0.0 0.10046267210672513 0.0 32-33 0.0 0.0 0.0 0.2090825697263895 0.0 34-35 0.0 0.0 0.0 0.3629965353257953 0.0 36-37 0.0 0.0 0.0 0.5908264128099925 0.0 38-39 0.0 0.0 0.0 0.8930730844473194 0.0 40-41 0.0 0.0 0.0 1.2288073134535547 0.0 42-43 0.0 0.0 0.0 1.526402935456311 0.0 44-45 0.0 0.0 0.0 1.8638544750968493 0.0 46-47 0.0 0.0 0.0 2.222199960788074 0.0 48-49 0.0 0.0 0.0 2.6017256109690354 0.0 50-51 0.0 0.0 0.0 2.9872618483700575 0.0 52-53 0.0 0.0 0.0 3.345034897183181 0.0 54-55 0.0 0.0 0.0 3.7126824821435473 0.0 56-57 0.0 0.0 0.0 4.119470438644068 0.0 58-59 0.0 0.0 0.0 4.56468322058712 0.0 60-61 0.0 0.0 0.0 5.015119489042842 0.0 62-63 0.0 0.0 0.0 5.457613195814914 0.0 64-65 0.0 0.0 0.0 5.90568816214847 0.0 66-67 0.0 0.0 0.0 6.389397324143813 0.0 68-69 0.0 0.0 0.0 6.918829881777474 0.0 70-71 0.0 0.0 0.0 7.4837535258533965 0.0 72-73 0.0 0.0 0.0 8.046172758587627 0.0 74-75 0.0 0.0 0.0 8.585980734636866 0.0 76-77 0.0 0.0 0.0 9.139527195760532 0.0 78-79 0.0 0.0 0.0 9.749673353206433 0.0 80-81 0.0 0.0 0.0 10.379568582946819 0.0 82-83 0.0 0.0 0.0 11.028354229664536 0.0 84-85 0.0 0.0 0.0 11.676853657943205 0.0 86-87 0.0 0.0 0.0 12.327213506075703 0.0 88-89 0.0 0.0 0.0 13.014495532845713 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5535 0.0 44.62891 1 AGCGTGC 55 0.0025796695 34.543453 8 GTATCAA 8645 0.0 34.453484 1 AGCGTCA 70 2.0523481E-4 33.929028 3 CATTCCG 180 5.456968E-12 31.664831 9 TGTATAG 410 0.0 30.120203 5 GTGTAGC 675 0.0 29.558073 1 TATCAAC 10615 0.0 27.925127 2 TAGCCCT 990 0.0 27.826668 4 GTATAGC 510 0.0 27.012104 1 ATCAACG 10935 0.0 26.975674 3 TCAACGC 11155 0.0 26.612082 4 CAACGCA 11355 0.0 26.143353 5 AACGCAG 11515 0.0 25.780094 6 GCGGTAT 95 0.0012254652 25.002127 1 GCGTCAG 95 0.0012264882 24.998549 4 ATGGGCG 140 1.778985E-5 23.748623 5 GAGTACT 7875 0.0 23.160563 12-13 GTAGCCC 645 0.0 22.829767 3 CGCAGAG 12945 0.0 22.82216 8 >>END_MODULE