FastQCFastQC Report
Thu 26 May 2016
SRR938017_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938017_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704538
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112111.5912555461877147No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97651.3860146649293579No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60120.8533251577629596No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32290.4583145266827339No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21640.30715163695925557No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16280.2310734126477209No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9780.13881437197141958No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8480.1203625638361593No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7620.10815598306975634No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7410.10517530637098355No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7370.10460755842836014No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7160.10162688172958734No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54800.048.546411
GTATCAA88150.035.0561681
GTATAGC5500.032.13081
CCGTACC300.00395160731.66608484-85
GTGTAGC5700.029.3275411
TATCAAC106600.029.1037942
ATCAACG109050.028.4063593
TCAACGC110700.028.0258754
TAGCCCT9500.028.0064494
GACCCGG856.3960766E-427.947616
CAACGCA111500.027.8247915
AACGCAG112650.027.540746
AGACCCG700.00837998227.1491095
TGTATAG4650.026.5652545
ATAGCCC5600.026.30073
CATGGGG21550.026.0152724
GAGTACT80400.024.70973612-13
CGCAGAG126100.024.4252328
ACGCAGA127350.024.2974557
GTAGCCC5900.024.1581043
Produced by FastQC (version 0.11.5)