##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938017_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 704538 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.986111465953574 33.0 31.0 34.0 31.0 34.0 2 32.1286176189219 34.0 31.0 34.0 30.0 34.0 3 32.14725678387823 34.0 31.0 34.0 30.0 34.0 4 35.67392532411311 37.0 35.0 37.0 35.0 37.0 5 35.7332663390761 37.0 35.0 37.0 35.0 37.0 6 35.802615330897694 37.0 37.0 37.0 35.0 37.0 7 35.76447686285197 37.0 36.0 37.0 35.0 37.0 8 35.76740928097562 37.0 37.0 37.0 35.0 37.0 9 37.44119266810307 39.0 38.0 39.0 35.0 39.0 10-11 37.38086093298019 39.0 38.0 39.0 35.0 39.0 12-13 37.296815785663796 39.0 38.0 39.0 35.0 39.0 14-15 38.49155758809319 40.0 38.0 41.0 34.5 41.0 16-17 38.437692502036796 40.0 38.0 41.0 34.5 41.0 18-19 38.52795094090028 40.0 38.0 41.0 35.0 41.0 20-21 38.51351382040429 40.0 38.5 41.0 34.5 41.0 22-23 38.362849839185394 40.0 38.0 41.0 34.0 41.0 24-25 38.31041689731427 40.0 38.0 41.0 34.0 41.0 26-27 38.24945496197508 40.0 38.0 41.0 34.0 41.0 28-29 38.12228864873151 40.0 38.0 41.0 34.0 41.0 30-31 37.79386349636216 40.0 37.5 41.0 34.0 41.0 32-33 37.38293889045019 40.0 35.5 41.0 33.0 41.0 34-35 37.11400307719385 40.0 35.0 41.0 33.0 41.0 36-37 36.86122253164485 40.0 35.0 41.0 32.5 41.0 38-39 36.55935946677113 40.0 35.0 41.0 31.0 41.0 40-41 36.23765432098766 39.0 35.0 41.0 30.5 41.0 42-43 35.782191308346746 39.0 35.0 41.0 29.5 41.0 44-45 35.163348179942034 39.0 35.0 41.0 25.5 41.0 46-47 34.47671026970866 39.0 35.0 41.0 14.0 41.0 48-49 33.87022417527514 39.0 35.0 41.0 2.0 41.0 50-51 33.09061328132762 38.5 34.0 40.5 2.0 41.0 52-53 33.05944179022281 39.0 34.0 40.0 2.0 41.0 54-55 33.12192387067837 39.0 34.0 41.0 2.0 41.0 56-57 32.89250757233819 39.0 34.0 41.0 2.0 41.0 58-59 32.64429313961773 39.0 34.0 41.0 2.0 41.0 60-61 32.30906920563547 38.5 33.0 40.5 2.0 41.0 62-63 31.93510499078829 38.0 33.0 40.0 2.0 41.0 64-65 31.56906937595985 37.5 32.0 40.0 2.0 41.0 66-67 31.17170046186295 37.0 31.5 40.0 2.0 41.0 68-69 30.71543763430787 36.5 31.0 39.0 2.0 41.0 70-71 30.25847079930394 36.0 30.5 39.0 2.0 40.5 72-73 29.77912830819629 35.0 30.0 38.5 2.0 40.0 74-75 29.276215761250633 35.0 29.0 37.5 2.0 39.5 76-77 28.738569814545137 35.0 28.5 37.0 2.0 39.0 78-79 28.191544671827494 35.0 27.5 36.5 2.0 39.0 80-81 27.659437815987214 34.0 26.0 36.0 2.0 37.5 82-83 27.14440172141176 34.0 25.5 35.5 2.0 37.0 84-85 26.649001189431942 34.0 24.5 35.0 2.0 37.0 86-87 26.157090887929392 34.0 21.5 35.0 2.0 36.0 88-89 25.637665392072535 33.5 19.0 35.0 2.0 36.0 90-91 25.075851834819414 33.0 10.0 35.0 2.0 35.0 92-93 24.526831058083452 32.5 2.0 35.0 2.0 35.0 94-95 24.02364315338562 32.0 2.0 35.0 2.0 35.0 96-97 23.33421972980875 32.0 2.0 35.0 2.0 35.0 98-99 22.49820094870681 31.0 2.0 34.0 2.0 35.0 100-101 20.09347331158859 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.44490544193783066 1101 2 0.4261347000340052 1101 3 0.35900164450897876 1101 4 0.25393441020053586 1101 5 0.3318261418143038 1101 6 0.20637129638549112 1101 7 0.17741833629183645 1101 8 0.4078931600529714 1101 9 0.42439344617380925 1101 10-11 0.4960861577149842 1101 12-13 0.3732757376221727 1101 14-15 0.5183764139686033 1101 16-17 0.7426243362307261 1101 18-19 0.5745406849843491 1101 20-21 0.609498857964681 1101 22-23 0.7682610062458792 1101 24-25 0.5350410471482974 1101 26-27 0.4957657217260092 1101 28-29 0.5839396024359971 1101 30-31 0.5269880826408055 1101 32-33 0.5936271340112569 1101 34-35 0.6070619129603827 1101 36-37 0.6173371367814582 1101 38-39 0.6104326686542478 1101 40-41 0.6903372876747227 1101 42-43 0.5159477060789257 1101 44-45 0.7196734677770849 1101 46-47 0.7236123926471834 1101 48-49 0.7167899658703121 1101 50-51 0.6888429547763835 1101 52-53 0.6833551388000387 1101 54-55 0.7491491908779508 1101 56-57 0.6090521984308026 1101 58-59 0.6727897337606876 1101 60-61 0.844098881935345 1101 62-63 0.6981995981874185 1101 64-65 0.647851465667614 1101 66-67 0.776563252717029 1101 68-69 0.6224192537775686 1101 70-71 0.8753019191419931 1101 72-73 0.6489234721752162 1101 74-75 0.6620738940517867 1101 76-77 0.7471889102916585 1101 78-79 0.8239122549897431 1101 80-81 0.88861818452839 1101 82-83 0.8783399934763523 1101 84-85 0.7146577183609857 1101 86-87 0.7076578862427247 1101 88-89 0.8901405596544336 1101 90-91 0.8343183606604008 1101 92-93 0.978504445920084 1101 94-95 0.9038647033857679 1101 96-97 0.9074490704336036 1101 98-99 1.367689841855828 1101 100-101 1.390762791309946 1102 1 0.46714648312164186 1102 2 0.3421847980906989 1102 3 0.3090645394595555 1102 4 0.39863736774891834 1102 5 0.44475724460545507 1102 6 0.3281720729152724 1102 7 0.22400269476333534 1102 8 0.3970722571390084 1102 9 0.3783909898040889 1102 10-11 0.48747524469821 1102 12-13 0.4359007846695775 1102 14-15 0.4645442791059864 1102 16-17 0.5515103527517979 1102 18-19 0.4814264439827909 1102 20-21 0.4984310799888121 1102 22-23 0.4364740467957944 1102 24-25 0.4644006081712533 1102 26-27 0.3844503258344858 1102 28-29 0.4983158033828943 1102 30-31 0.2527735266692517 1102 32-33 0.41918650288270953 1102 34-35 0.37448554759519226 1102 36-37 0.5348159902114631 1102 38-39 0.5513522720694013 1102 40-41 0.5801287438661618 1102 42-43 0.5461570917883947 1102 44-45 0.580914578293914 1102 46-47 0.38657098102697063 1102 48-49 0.3419348223509715 1102 50-51 0.22045261979886988 1102 52-53 0.2091234971923086 1102 54-55 -0.016347976373097595 1102 56-57 0.025096106039598 1102 58-59 0.2742709841321158 1102 60-61 0.12589549848414805 1102 62-63 0.22427624088467368 1102 64-65 0.32429408764419065 1102 66-67 0.13097877538892178 1102 68-69 0.04618017372764527 1102 70-71 0.07269177869310894 1102 72-73 0.18498035496279996 1102 74-75 0.21004986520925328 1102 76-77 -0.03260323922994601 1102 78-79 -0.01640761783369271 1102 80-81 0.09452238610918329 1102 82-83 0.08722675285116566 1102 84-85 0.1108067645367754 1102 86-87 0.29202007201559255 1102 88-89 0.110773074858173 1102 90-91 0.18031982655846335 1102 92-93 0.15642410876353097 1102 94-95 0.006385949002932989 1102 96-97 0.25105607108731576 1102 98-99 0.28643278474523015 1102 100-101 0.07361128876443246 1103 1 0.11306230298082554 1103 2 0.012215127126601999 1103 3 0.13263195573776443 1103 4 0.048668574473964554 1103 5 -0.08193669543862825 1103 6 -0.1418085204025843 1103 7 0.10776983408186425 1103 8 0.09254825721702531 1103 9 0.13708011230998807 1103 10-11 0.16456975231459836 1103 12-13 0.008034642015829263 1103 14-15 0.1432320363176629 1103 16-17 0.13979369031104483 1103 18-19 0.1686375379735452 1103 20-21 0.16473732523466111 1103 22-23 0.05923472047097533 1103 24-25 0.19780740733647662 1103 26-27 0.24021064843400808 1103 28-29 0.3227999172094229 1103 30-31 0.24018112550680115 1103 32-33 0.14039464198668838 1103 34-35 0.19233213728526977 1103 36-37 0.30026331911084014 1103 38-39 0.23807697999395572 1103 40-41 0.38052394778260634 1103 42-43 0.36958554061911997 1103 44-45 0.21581174238277612 1103 46-47 0.16178138261851416 1103 48-49 -0.018718617215256472 1103 50-51 -0.267743680253794 1103 52-53 -0.09869549405030398 1103 54-55 -0.32306254211201235 1103 56-57 -0.3022173362572147 1103 58-59 -0.23021742061911254 1103 60-61 -0.1544603557024331 1103 62-63 -0.25465190563090445 1103 64-65 -0.25686290168700765 1103 66-67 -0.2885712626442718 1103 68-69 -0.21935248606053648 1103 70-71 -0.22573350070460307 1103 72-73 -0.155364333067471 1103 74-75 -0.05985173608582528 1103 76-77 -0.003243318417009533 1103 78-79 -0.03646159571592733 1103 80-81 -0.13093157421926804 1103 82-83 -0.3150705984780444 1103 84-85 -0.05249217638499459 1103 86-87 -0.2789520662302003 1103 88-89 -0.4733631815050714 1103 90-91 -0.17387650275050248 1103 92-93 -0.16236112070341946 1103 94-95 -0.03140588263678623 1103 96-97 0.10718575453409684 1103 98-99 0.26171242625797575 1103 100-101 0.059351278232163907 1104 1 0.3684047712470253 1104 2 0.18715918024862077 1104 3 0.07594217659035962 1104 4 0.09855071916543778 1104 5 0.23546324219436343 1104 6 0.16337023445837673 1104 7 0.20217101905507917 1104 8 0.11998515647595553 1104 9 0.1298414117450193 1104 10-11 0.2333500186584203 1104 12-13 0.05767029943750401 1104 14-15 0.22165511394624815 1104 16-17 0.38587970175721864 1104 18-19 0.4010693011256521 1104 20-21 0.47289800283390093 1104 22-23 0.31540148802545076 1104 24-25 0.39054210907541176 1104 26-27 0.3376224174559397 1104 28-29 0.4507061740881326 1104 30-31 0.22884156464393612 1104 32-33 0.3394038778073636 1104 34-35 0.43276613831248767 1104 36-37 0.30144875091068 1104 38-39 0.417018712696283 1104 40-41 0.6019039096286676 1104 42-43 0.5779062629758585 1104 44-45 0.5504462992101296 1104 46-47 0.740690556072181 1104 48-49 0.585407923616792 1104 50-51 0.9175310556035754 1104 52-53 0.8107095430030355 1104 54-55 0.8254053388770544 1104 56-57 0.8706355876550305 1104 58-59 0.6153932716547246 1104 60-61 0.628824597338884 1104 62-63 0.8356667622831004 1104 64-65 0.643942595781624 1104 66-67 0.5926367771972423 1104 68-69 0.6284869097131782 1104 70-71 0.8559007901457925 1104 72-73 0.7037699119248266 1104 74-75 0.623683831453981 1104 76-77 0.39904798138488573 1104 78-79 0.22032196323924325 1104 80-81 0.39513646085300635 1104 82-83 0.6378973375407391 1104 84-85 0.6727605017278542 1104 86-87 0.6780112564640532 1104 88-89 1.081783472688194 1104 90-91 1.0201800586259715 1104 92-93 0.6388589339594297 1104 94-95 0.8902203371885875 1104 96-97 0.8280979637937627 1104 98-99 1.3022555695553564 1104 100-101 1.2355782116095213 1105 1 0.3125069754474801 1105 2 0.3008912451269765 1105 3 0.13980680541131818 1105 4 0.08181777052746497 1105 5 -0.04598170478006125 1105 6 0.12798735860060617 1105 7 0.062179596721954056 1105 8 0.0076567098134461276 1105 9 -0.03214002930301518 1105 10-11 0.21072967139287613 1105 12-13 0.11578074339674771 1105 14-15 0.23475230151122872 1105 16-17 0.18681934617394091 1105 18-19 0.17138277586051487 1105 20-21 0.13712458320404153 1105 22-23 0.05565738842054202 1105 24-25 0.01007709555909031 1105 26-27 0.008421959980374538 1105 28-29 0.10795365277393643 1105 30-31 0.1055018630427682 1105 32-33 0.1215603812384316 1105 34-35 0.1654808399136556 1105 36-37 0.27534535103544755 1105 38-39 0.3310113566971111 1105 40-41 0.25142823901107647 1105 42-43 0.2184987718134508 1105 44-45 0.2215220166149905 1105 46-47 0.3142732915681208 1105 48-49 0.27202091488316427 1105 50-51 0.27682157858132683 1105 52-53 0.3816897363904346 1105 54-55 0.3313122322020732 1105 56-57 0.6569607072225097 1105 58-59 0.2664609312513093 1105 60-61 0.09444569839939021 1105 62-63 0.1323728082386033 1105 64-65 0.2113650068858064 1105 66-67 0.4366694503101556 1105 68-69 0.4908510966543318 1105 70-71 0.309587887255379 1105 72-73 0.1145459308476795 1105 74-75 0.4970917644961901 1105 76-77 0.36618966884589454 1105 78-79 0.3443829686457711 1105 80-81 0.4149625681518181 1105 82-83 0.28647292498095567 1105 84-85 0.338783843378927 1105 86-87 0.2631348528458375 1105 88-89 0.07705925767467647 1105 90-91 0.14825047347313358 1105 92-93 0.30219525647970613 1105 94-95 0.4167396239425898 1105 96-97 0.36177180450908963 1105 98-99 0.24375848076350692 1105 100-101 0.06920071628824687 1106 1 0.1775164928587465 1106 2 0.24037679596624173 1106 3 -0.05406947842129739 1106 4 0.10420964357435736 1106 5 -0.07382954834842792 1106 6 0.04726342460156019 1106 7 0.13555222118628052 1106 8 0.06158938558018434 1106 9 0.21132451197811974 1106 10-11 -0.39730058074217567 1106 12-13 -0.11513710149489498 1106 14-15 -0.10157061865448469 1106 16-17 0.07870562583314467 1106 18-19 0.05834952090587109 1106 20-21 0.10356629287718988 1106 22-23 -0.06572675872779854 1106 24-25 -0.1251984004150941 1106 26-27 -0.14115713632360638 1106 28-29 -0.32031967506271286 1106 30-31 -0.21475546332809614 1106 32-33 -0.23479796201947778 1106 34-35 -0.17883059828424308 1106 36-37 -0.16075363004169674 1106 38-39 -0.04775921770664837 1106 40-41 0.179255724480484 1106 42-43 0.054106104800702326 1106 44-45 0.25611042674925244 1106 46-47 0.5240665550731833 1106 48-49 0.6273878187216866 1106 50-51 0.5378388727863879 1106 52-53 0.633863472406965 1106 54-55 0.22134897857069546 1106 56-57 0.4582188377382863 1106 58-59 0.7578352047216512 1106 60-61 0.3897022613415473 1106 62-63 0.7833118255531666 1106 64-65 0.7787421712062041 1106 66-67 0.4954870519225132 1106 68-69 0.3431815900328594 1106 70-71 0.7757954787904815 1106 72-73 0.8429526042325186 1106 74-75 0.9507088897290359 1106 76-77 1.0941372670991711 1106 78-79 1.0447402532825336 1106 80-81 1.1488598208263632 1106 82-83 0.9256429252863256 1106 84-85 0.8745316472490003 1106 86-87 0.7046439987217994 1106 88-89 0.7029435625982856 1106 90-91 0.5916325644534766 1106 92-93 0.5548606405861385 1106 94-95 0.6474610131792637 1106 96-97 0.48434588254168176 1106 98-99 0.11632899612878234 1106 100-101 0.17026748067378605 1107 1 0.30707441810980285 1107 2 0.3486225206318849 1107 3 0.17985648794073938 1107 4 0.17067426912377925 1107 5 0.2665049489174294 1107 6 0.28352541294968603 1107 7 0.13206230598708402 1107 8 0.254122021181459 1107 9 0.17937315243590746 1107 10-11 0.18518019554748122 1107 12-13 0.21105326264970614 1107 14-15 0.31962205139416966 1107 16-17 0.31723304726769896 1107 18-19 0.4444143309276072 1107 20-21 0.4413125304888439 1107 22-23 0.5568965130206394 1107 24-25 0.41190218406099177 1107 26-27 0.5099049913199423 1107 28-29 0.6686119854705623 1107 30-31 0.5745893775645285 1107 32-33 0.5850968134607655 1107 34-35 0.47319906787731725 1107 36-37 0.35728136322882875 1107 38-39 0.29178938980965796 1107 40-41 0.348727437155425 1107 42-43 0.261457703403984 1107 44-45 0.31702864715603596 1107 46-47 0.13258334111351644 1107 48-49 0.1874646194445191 1107 50-51 0.03624921076604437 1107 52-53 0.11467552710865903 1107 54-55 0.19302494728569286 1107 56-57 0.17418698802675436 1107 58-59 0.20342622204887562 1107 60-61 0.08127865264875567 1107 62-63 -0.06635828898908613 1107 64-65 -0.018312859899971556 1107 66-67 -0.21392386018018428 1107 68-69 0.06195823734248407 1107 70-71 0.3327061320672158 1107 72-73 0.03866644624514848 1107 74-75 0.17565500686896485 1107 76-77 -0.13116555928037243 1107 78-79 0.12097908847157157 1107 80-81 0.09729510021722731 1107 82-83 0.10950536163866786 1107 84-85 0.13571434073317334 1107 86-87 0.6122035992827435 1107 88-89 0.6282228593446035 1107 90-91 0.5931177549023303 1107 92-93 0.38269525550016326 1107 94-95 0.3163783676955383 1107 96-97 0.2134285092016981 1107 98-99 0.07925758839788699 1107 100-101 -0.20600053126044315 1108 1 0.30842214927599443 1108 2 0.3511582382620091 1108 3 0.1950303985094095 1108 4 0.2626045910552577 1108 5 0.22131923192995373 1108 6 0.23132929052281526 1108 7 0.21630284845805647 1108 8 0.2406447095195503 1108 9 0.3826694017404719 1108 10-11 0.39397294791197623 1108 12-13 0.17820093836429152 1108 14-15 0.2786686509435796 1108 16-17 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-1.098317780219162 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4560.0 3 701.0 4 572.0 5 539.0 6 584.0 7 684.0 8 861.0 9 1031.0 10 1434.0 11 2148.0 12 3529.0 13 5301.0 14 6441.0 15 8923.0 16 13029.0 17 20290.0 18 16773.0 19 9220.0 20 7205.0 21 6220.0 22 5750.0 23 5837.0 24 6178.0 25 6616.0 26 7276.0 27 8257.0 28 9553.0 29 11436.0 30 13563.0 31 16226.0 32 20040.0 33 26199.0 34 35238.0 35 52736.0 36 75830.0 37 132794.0 38 141750.0 39 19212.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.63773898183717 19.771427593299737 15.321369323312132 19.269464101550966 2 15.958465047189774 21.979953932102394 40.787272536205336 21.274308484502498 3 18.953276496511254 27.972322938813072 31.74880453785642 21.325596026819255 4 11.308910041728094 19.387372493544785 38.14200618677938 31.16171127794774 5 11.097963735022763 39.70041975326179 34.20328558136892 14.998330930346526 6 27.467791644228214 38.160951551414726 18.413503140909693 15.957753663447372 7 24.37219476166127 37.685784898585304 20.662888472245893 17.279131867507527 8 25.97726091439186 33.77120605769695 20.003351501501072 20.248181526410118 9 27.427779403035096 15.910117274149982 21.161323672677593 35.500779650137325 10-11 23.84250825448164 28.17837492711412 26.649280587035868 21.32983623136838 12-13 26.328341794652104 27.346328668596215 27.305123253920556 19.020206282831126 14-15 23.024278014869306 27.41825076157271 29.038110589588644 20.519360633969338 16-17 18.74557952592635 30.377710869040346 29.857266620272398 21.019442984760904 18-19 17.954256734062323 27.101347700090393 35.72047880669 19.22391675915728 20-21 20.938446372463794 25.711998795461387 35.8117283424526 17.53782648962222 22-23 19.616226948904114 26.870859892513437 35.20575553055868 18.30715762802377 24-25 18.5410353777505 26.754929913611974 36.2887243808574 18.41531032778012 26-27 18.481932091593688 27.136021338908566 35.796360000340876 18.58568656915687 28-29 18.047712113809126 27.406981830990716 35.91454372947606 18.63076232572409 30-31 18.95722513795479 27.11221743237197 35.99657687472924 17.933980554944 32-33 18.036159671838693 27.32868968416747 36.015504712773634 18.61964593122021 34-35 18.498843444992797 27.677707144789892 35.75178916254338 18.07166024767393 36-37 18.721407033177428 27.392435051976882 35.08982860908753 18.79632930575815 38-39 18.27971265355271 27.718083542898203 35.30608303738656 18.696120766162526 40-41 19.10382884577972 27.22445246923135 35.082653253943796 18.58906543104513 42-43 18.58002063271286 27.473103277874927 34.79608333457584 19.150792754836367 44-45 19.231945916363152 27.819724996291118 34.10927603367735 18.839053053668383 46-47 19.092243671744228 27.83616174551815 33.22978500470653 19.841809578031093 48-49 19.097303808997147 27.89497483834283 33.040248003288156 19.967473349371865 50-51 19.528428794025675 28.591639780080147 31.67655170210727 20.20337972378691 52-53 19.474197933563094 28.852740637677865 31.49687123989764 20.1761901888614 54-55 19.83769571069175 28.496243376928714 31.31527239066608 20.35078852171345 56-57 19.86634476208189 28.881826422819923 30.777476238713874 20.47435257638431 58-59 19.965487035988037 28.752876671876976 31.03795858160378 20.24367771053121 60-61 19.994207170330487 28.507892020519108 31.04743731178628 20.450463497364122 62-63 19.59016154895056 28.241218229144117 31.374951288295915 20.793668933609407 64-65 19.57419532527712 28.39630088133745 31.12499716063965 20.904506632745775 66-67 19.517423380989086 28.66173499880044 30.813554846705195 21.007286773505275 68-69 19.99865132489583 28.24210847606812 30.855201981842573 20.904038217193477 70-71 19.830451915225957 28.173524086762043 30.77148638574319 21.224537612268808 72-73 19.872660032246774 27.987246124103933 30.994105130730066 21.145988712919227 74-75 20.08394191934789 28.134173604440743 30.425751086367743 21.356133389843624 76-77 19.644108258213173 28.212881980602567 30.72348895433731 21.419520806846958 78-79 19.719490511865935 28.26459596909569 30.33860589907479 21.67730761996359 80-81 19.815791845921577 28.43841143180813 29.99756537465903 21.748231347611263 82-83 19.99838157723816 28.631176359102454 30.001157030307805 21.369285033351577 84-85 20.235828063567958 28.30923402017145 29.788270066573304 21.666667849687293 86-87 20.078442283314903 28.305016416666785 29.783864964576058 21.832676335442255 88-89 20.663140331570165 28.098548049274026 29.651105825552914 21.587205793602894 90-91 20.76831806339881 28.179899071448812 29.50487920739773 21.546903657754648 92-93 20.796286146742357 27.975157678316354 29.741850565540506 21.48670560940078 94-95 20.793826957993122 28.191593227945127 29.427394542563928 21.587185271497823 96-97 21.238948734624792 27.846490717363086 29.16043043489374 21.754130113118382 98-99 21.438221615140012 27.580527333253347 28.894565296874326 22.08668575473232 100-101 21.98781819422644 27.234966104779453 29.125363975522646 21.651851725471463 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3214.0 1 1804.5 2 505.5 3 628.0 4 457.0 5 375.0 6 731.0 7 1416.0 8 2021.0 9 2206.5 10 1862.0 11 1507.0 12 1589.0 13 1744.0 14 1875.0 15 2082.0 16 2309.5 17 2526.5 18 2827.0 19 3142.0 20 3476.0 21 3911.5 22 4389.5 23 4825.0 24 5417.0 25 6341.5 26 7584.5 27 8939.0 28 9956.5 29 11189.0 30 12651.0 31 13973.5 32 15345.0 33 16817.5 34 18865.5 35 20945.0 36 22882.0 37 24255.0 38 23845.0 39 22921.0 40 22825.0 41 23024.0 42 23181.0 43 23456.0 44 23597.5 45 23760.5 46 24147.5 47 24364.5 48 24811.0 49 25153.0 50 25304.0 51 23965.0 52 20785.5 53 18119.0 54 16308.5 55 14508.0 56 12538.5 57 10399.0 58 8347.5 59 6467.0 60 4702.0 61 3449.5 62 2565.0 63 1803.0 64 1213.5 65 907.0 66 688.5 67 519.0 68 398.5 69 292.0 70 220.0 71 185.0 72 184.5 73 164.0 74 122.5 75 91.0 76 66.5 77 44.0 78 31.5 79 24.0 80 17.0 81 14.0 82 9.5 83 4.5 84 3.5 85 4.5 86 2.5 87 1.0 88 1.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4955020169245662 2 0.05109731483610536 3 0.05918772301848871 4 0.06472326545906679 5 0.07849115306768407 6 0.10801404608410049 7 0.07068461885661242 8 0.053368306606598935 9 0.053368306606598935 10-11 0.07472982294780409 12-13 0.10602692828491864 14-15 0.05720060521930684 16-17 0.06074902986070304 18-19 0.0585490065830374 20-21 0.07579435034022296 22-23 0.06387164354513171 24-25 0.08814286809228175 26-27 0.06699425722956037 28-29 0.06841362708611884 30-31 0.07139430378489166 32-33 0.07011687091398902 34-35 0.07203302022034298 36-37 0.0674200681865279 38-39 0.04272303268241032 40-41 0.046981142252085765 42-43 0.04492305596007597 44-45 0.020935705384237613 46-47 0.027961586174202097 48-49 0.027322869738750786 50-51 0.027038995767439084 52-53 0.026329310839159847 54-55 0.025832531389364376 56-57 0.02675512179612739 58-59 0.023419602633214957 60-61 0.031084199858630764 62-63 0.01965827251333498 64-65 0.021858295791000626 66-67 0.01575500540779915 68-69 0.020296988948786298 70-71 0.0444262765102805 72-73 0.03995526146212128 74-75 0.03427778203588735 76-77 0.02363250811169873 78-79 0.014974351986691989 80-81 0.016677595814562168 82-83 0.02107764236989346 84-85 0.0179550286854648 86-87 0.010077525981565224 88-89 0.0444262765102805 90-91 0.05343927509942686 92-93 0.03633586832789715 94-95 0.0538650860563944 96-97 0.045419835409871434 98-99 0.036690710792036764 100-101 0.1694727608730828 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 704538.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.764392323147234 #Duplication Level Percentage of deduplicated Percentage of total 1 79.08460688657554 49.63697293350899 2 12.520258540421494 15.716528380364986 3 4.187778229986749 7.885300673676704 4 1.7913199357308645 4.497244288899474 5 0.867753386841186 2.723200700571997 6 0.48940225285042493 1.8430221001041704 7 0.27324250818471696 1.2004929988146442 8 0.1790501305080701 0.8990378109375379 9 0.11215218053766854 0.6335247113246398 >10 0.4318415938158851 4.682588696408843 >50 0.0345739004497996 1.5550290791942372 >100 0.02440491163408834 2.8842463092494195 >500 0.0022597140396973976 1.0193291808641438 >1k 6.779142119092194E-4 0.9957854708288791 >5k 4.519428079394795E-4 2.237645260399833 >10k+ 2.2597140396973976E-4 1.590051404851526 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11211 1.5912555461877147 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9765 1.3860146649293579 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6012 0.8533251577629596 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3229 0.4583145266827339 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2164 0.30715163695925557 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1628 0.2310734126477209 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 978 0.13881437197141958 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 848 0.1203625638361593 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 762 0.10815598306975634 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 741 0.10517530637098355 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 737 0.10460755842836014 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 716 0.10162688172958734 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 7.096849282792412E-5 14-15 0.0 0.0 0.0 0.0 1.4193698565584823E-4 16-17 0.0 0.0 0.0 1.4193698565584823E-4 1.4193698565584823E-4 18-19 0.0 0.0 0.0 1.4193698565584823E-4 1.4193698565584823E-4 20-21 0.0 0.0 0.0 5.677479426233929E-4 1.4193698565584823E-4 22-23 0.0 0.0 0.0 0.0020580862920097994 1.4193698565584823E-4 24-25 0.0 0.0 0.0 0.006883943804308639 1.4193698565584823E-4 26-27 0.0 0.0 0.0 0.01653565882890632 1.4193698565584823E-4 28-29 0.0 0.0 0.0 0.04094882036171221 1.4193698565584823E-4 30-31 0.0 0.0 0.0 0.10482046390684392 1.4193698565584823E-4 32-33 0.0 0.0 0.0 0.21184095109135348 1.4193698565584823E-4 34-35 0.0 0.0 0.0 0.3833717982564461 1.4193698565584823E-4 36-37 0.0 0.0 0.0 0.6126709985834689 1.4193698565584823E-4 38-39 0.0 0.0 0.0 0.9182613287005101 1.4193698565584823E-4 40-41 0.0 0.0 0.0 1.2515293710204416 1.4193698565584823E-4 42-43 0.0 0.0 0.0 1.5374614286241481 1.4193698565584823E-4 44-45 0.0 0.0 0.0 1.873000462714573 1.4193698565584823E-4 46-47 0.0 0.0 0.0 2.2284106747968173 1.4193698565584823E-4 48-49 0.0 0.0 0.0 2.5983694279087857 1.4193698565584823E-4 50-51 0.0 0.0 0.0 2.991889720639625 1.4193698565584823E-4 52-53 0.0 0.0 0.0 3.354680655975973 1.4193698565584823E-4 54-55 0.0 0.0 0.0 3.734787903562334 1.4193698565584823E-4 56-57 0.0 0.0 0.0 4.1514439249550765 1.4193698565584823E-4 58-59 0.0 0.0 0.0 4.585487227090661 1.4193698565584823E-4 60-61 0.0 0.0 0.0 5.034362944227281 1.4193698565584823E-4 62-63 0.0 0.0 0.0 5.476709560023732 1.4193698565584823E-4 64-65 0.0 0.0 0.0 5.929204670294576 1.4193698565584823E-4 66-67 0.0 0.0 0.0 6.401996769514206 1.4193698565584823E-4 68-69 0.0 0.0 0.0 6.947318668403975 1.4193698565584823E-4 70-71 0.0 0.0 0.0 7.522163460310161 1.4193698565584823E-4 72-73 0.0 0.0 0.0 8.08423392350732 1.4193698565584823E-4 74-75 0.0 0.0 0.0 8.630052601846884 1.4193698565584823E-4 76-77 0.0 0.0 0.0 9.187226239038917 1.4193698565584823E-4 78-79 0.0 0.0 0.0 9.800961765014804 1.4193698565584823E-4 80-81 0.0 0.0 0.0 10.437620114174111 1.4193698565584823E-4 82-83 0.0 0.0 0.0 11.08208499754449 1.4193698565584823E-4 84-85 0.0 0.0 0.0 11.721582086416914 1.4193698565584823E-4 86-87 0.0 0.0 0.0 12.359233994475812 1.4193698565584823E-4 88-89 0.0 0.0 0.0 13.026408795551127 1.4193698565584823E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5480 0.0 48.54641 1 GTATCAA 8815 0.0 35.056168 1 GTATAGC 550 0.0 32.1308 1 CCGTACC 30 0.003951607 31.666084 84-85 GTGTAGC 570 0.0 29.327541 1 TATCAAC 10660 0.0 29.103794 2 ATCAACG 10905 0.0 28.406359 3 TCAACGC 11070 0.0 28.025875 4 TAGCCCT 950 0.0 28.006449 4 GACCCGG 85 6.3960766E-4 27.94761 6 CAACGCA 11150 0.0 27.824791 5 AACGCAG 11265 0.0 27.54074 6 AGACCCG 70 0.008379982 27.149109 5 TGTATAG 465 0.0 26.565254 5 ATAGCCC 560 0.0 26.3007 3 CATGGGG 2155 0.0 26.015272 4 GAGTACT 8040 0.0 24.709736 12-13 CGCAGAG 12610 0.0 24.425232 8 ACGCAGA 12735 0.0 24.297455 7 GTAGCCC 590 0.0 24.158104 3 >>END_MODULE