FastQCFastQC Report
Thu 26 May 2016
SRR938017_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938017_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704538
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105571.4984287575687898No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95191.3510981664580193No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60260.8553122755621414No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29750.4222625323261485No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT22810.32375826428098986No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21590.30644195203097635No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16060.22795079896329226No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT13560.1924665525493302RNA PCR Primer, Index 26 (100% over 22bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8890.12618198024804905No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8530.12107224876443853No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT7560.10730436115582126RNA PCR Primer, Index 26 (100% over 24bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7350.10432368445704844No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7240.10276237761483412No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7190.10205269268655488No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7090.10063332282999639No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACCG250.00502096156.9985969
GGTATCA52100.050.33221
TACCGTT308.2894454E-539.5851730-31
GTATCAA83500.035.444281
CATTCCG1201.5047408E-731.6658869
ATGGGCG1751.5097612E-1029.8564075
TATCAAC103150.028.6461142
GTATAGC6100.028.0360741
ATCAACG106300.027.748613
TCAACGC107700.027.3879034
ATGCCGC350.0083932127.14218994-95
CAACGCA109350.026.9746445
AACGCAG111400.026.478256
TAGCCCT10550.025.6628764
GTGGTAT23550.024.6101061
GTAGCCC6000.023.7494143
GAGTACT80000.023.69004212-13
ATAGCCC6250.023.5594183
CTGTATA5650.023.5392444
CGCAGAG125050.023.3220968
Produced by FastQC (version 0.11.5)