FastQCFastQC Report
Thu 26 May 2016
SRR938016_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938016_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730632
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110281.5093781821765266No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98711.3510221287871322No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59760.8179220181979437No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32870.4498844835703884No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21340.29207590141138084No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16000.21898849215473726No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9580.13111935967764893No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9040.12372849806742654No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8340.1141477515356568No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8040.11004171730775547No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7740.10593568307985415No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7540.10319832692791994No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7310.1000503673531956No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53400.048.5911031
GTATCAA87850.038.42431
GCGGTAT753.0357437E-431.7402421
CGGTATC600.00393040131.6988832
TATCAAC110300.030.4343722
ATCAACG111750.030.0820263
TCAACGC112750.029.8995634
CAACGCA113700.029.5661055
AACGCAG115400.029.1185746
CGGATCA350.00838856627.14533494-95
ATAGCCC5750.026.4616723
TAGCCCT10750.026.0962874
GTATAGC5700.025.8933541
ACGCAGA130450.025.6862987
ACGAAGG1850.025.67801994-95
CGCAGAG129100.025.6586198
GCAGAGT130300.024.546949
GTAGGAC5250.024.453423
GAGTACT80100.024.15172412-13
GACGTGG2801.8189894E-1223.764387
Produced by FastQC (version 0.11.5)