##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938016_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 730632 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.05871355210284 33.0 31.0 34.0 31.0 34.0 2 32.13127538897831 34.0 31.0 34.0 30.0 34.0 3 32.09281964107786 34.0 31.0 34.0 30.0 34.0 4 35.618734191768226 37.0 35.0 37.0 35.0 37.0 5 35.66954225930427 37.0 35.0 37.0 35.0 37.0 6 35.74639900798213 37.0 37.0 37.0 35.0 37.0 7 35.701532371973855 37.0 36.0 37.0 35.0 37.0 8 35.71673291068554 37.0 36.0 37.0 35.0 37.0 9 37.364606258691104 39.0 38.0 39.0 35.0 39.0 10-11 37.30231908813192 39.0 38.0 39.0 35.0 39.0 12-13 37.18298404668835 39.0 37.0 39.0 34.5 39.0 14-15 38.329983493742404 40.0 38.0 41.0 34.0 41.0 16-17 38.19920561924471 40.0 38.0 41.0 34.0 41.0 18-19 38.475317259578006 40.0 38.5 41.0 34.0 41.0 20-21 38.53820185811736 40.0 38.5 41.0 35.0 41.0 22-23 38.408065893637286 40.0 38.0 41.0 34.0 41.0 24-25 38.31319391978452 40.0 38.0 41.0 34.0 41.0 26-27 38.1438111114761 40.0 38.0 41.0 34.0 41.0 28-29 37.95774206440451 40.0 38.0 41.0 34.0 41.0 30-31 37.61694396084486 40.0 37.0 41.0 33.5 41.0 32-33 37.1703319865541 40.0 35.5 41.0 33.0 41.0 34-35 36.88409897184903 40.0 35.0 41.0 32.5 41.0 36-37 36.60404896035213 39.0 35.0 41.0 31.5 41.0 38-39 36.27723600937271 39.0 35.0 41.0 30.5 41.0 40-41 35.990655350436334 39.0 35.0 41.0 30.0 41.0 42-43 35.59924626898356 39.0 35.0 41.0 28.0 41.0 44-45 34.96620733830437 39.0 35.0 41.0 24.0 41.0 46-47 34.17190938803666 39.0 35.0 41.0 11.5 41.0 48-49 33.431528457554556 39.0 34.0 41.0 2.0 41.0 50-51 32.58598788446167 38.5 33.5 39.5 2.0 40.5 52-53 32.552636621445544 38.0 33.0 40.0 2.0 40.5 54-55 32.59081384335754 38.0 33.0 40.0 2.0 41.0 56-57 32.308174292940905 38.0 33.0 40.0 2.0 41.0 58-59 31.966141641756728 38.0 32.5 40.0 2.0 41.0 60-61 31.5684599086818 38.0 31.5 40.0 2.0 41.0 62-63 31.12055795530445 37.0 31.0 40.0 2.0 41.0 64-65 30.703876917517984 37.0 30.5 40.0 2.0 41.0 66-67 30.266864851252066 36.0 29.5 39.0 2.0 41.0 68-69 29.701212785643115 35.5 28.5 39.0 2.0 40.0 70-71 29.222324644964907 35.0 28.0 38.5 2.0 40.0 72-73 28.621350419910435 35.0 26.5 37.5 2.0 40.0 74-75 28.044697604265895 34.5 26.0 37.0 2.0 39.0 76-77 27.38644830776643 34.0 24.5 36.5 2.0 39.0 78-79 26.740311127900227 34.0 22.5 36.0 2.0 38.5 80-81 26.093735286710682 33.5 19.0 35.0 2.0 37.0 82-83 25.455122414567114 33.0 15.5 35.0 2.0 37.0 84-85 24.798759156456327 32.0 7.0 35.0 2.0 36.0 86-87 24.13937591017092 32.0 2.0 35.0 2.0 36.0 88-89 23.397875401022674 31.0 2.0 34.5 2.0 35.0 90-91 22.64456593743499 31.0 2.0 34.0 2.0 35.0 92-93 21.851787904170635 30.0 2.0 34.0 2.0 35.0 94-95 20.9414835375401 29.0 2.0 34.0 2.0 35.0 96-97 19.865591706905803 28.0 2.0 34.0 2.0 35.0 98-99 18.52915968640848 25.5 2.0 34.0 2.0 35.0 100-101 14.683773089599143 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.05792279272734646 1101 2 0.22907736053657146 1101 3 0.06712851398469155 1101 4 0.017950351327421288 1101 5 -0.02573724588896198 1101 6 0.036717395436852485 1101 7 0.10306159523388914 1101 8 0.06476869753034009 1101 9 0.08027156192181195 1101 10-11 0.14073224173171894 1101 12-13 0.14140896629657362 1101 14-15 0.25961641845179884 1101 16-17 0.39856843583519463 1101 18-19 0.3418052893221599 1101 20-21 0.3272390673149843 1101 22-23 0.32832638758700483 1101 24-25 0.3145975208683325 1101 26-27 0.3150950949674467 1101 28-29 0.3630971709283699 1101 30-31 0.2883597337165469 1101 32-33 0.3578515620330691 1101 34-35 0.4412599557761183 1101 36-37 0.15456683383317227 1101 38-39 0.36342049654329855 1101 40-41 0.4346347718256709 1101 42-43 0.4217149897653769 1101 44-45 0.5486385802336855 1101 46-47 0.5164019248201939 1101 48-49 0.5196508083341413 1101 50-51 0.3892782915734827 1101 52-53 0.5878329322405875 1101 54-55 0.5264433916673283 1101 56-57 0.3515635850354215 1101 58-59 0.3263164472939195 1101 60-61 0.47330047854980606 1101 62-63 0.3116374983038348 1101 64-65 0.09502131544164527 1101 66-67 0.07913832274686072 1101 68-69 0.12048259772333836 1101 70-71 0.2930681375057951 1101 72-73 0.14832555523601698 1101 74-75 0.13966206000907277 1101 76-77 0.08872709325591899 1101 78-79 -0.22324794561572858 1101 80-81 -0.10979502795062146 1101 82-83 -0.2162563025824511 1101 84-85 0.034036750901030643 1101 86-87 0.22614871942563752 1101 88-89 0.23548006901982532 1101 90-91 -0.2590078872767059 1101 92-93 -0.10164629949001025 1101 94-95 0.25512503182806157 1101 96-97 0.17352325256556966 1101 98-99 0.18631010382777546 1101 100-101 -0.007145696050288919 1102 1 0.13332793561369982 1102 2 0.14314617092458803 1102 3 0.28740610715478 1102 4 0.28081523553579757 1102 5 0.2592584087299272 1102 6 0.3065885676673119 1102 7 0.3630585279060412 1102 8 0.36472268761264104 1102 9 0.31170144791946086 1102 10-11 0.3649321803851606 1102 12-13 0.4041396549116669 1102 14-15 0.3281993129868468 1102 16-17 0.34727504592670044 1102 18-19 0.34942759902002507 1102 20-21 0.18196275219817437 1102 22-23 0.3622420871933727 1102 24-25 0.3819803233835444 1102 26-27 0.35205128330137825 1102 28-29 0.322355388810891 1102 30-31 0.3008309775179896 1102 32-33 0.3640271154301331 1102 34-35 0.5117113641908233 1102 36-37 0.5969406899754972 1102 38-39 0.4274560775463172 1102 40-41 0.6830755468371663 1102 42-43 0.9424961359235482 1102 44-45 0.8629706695951711 1102 46-47 0.6916166377694353 1102 48-49 0.7395654343757485 1102 50-51 0.5742765022989005 1102 52-53 0.4759853784345438 1102 54-55 0.48466383029520443 1102 56-57 0.2205631249362412 1102 58-59 0.3283690008443543 1102 60-61 0.4801521221263023 1102 62-63 0.28702219233788284 1102 64-65 0.49214058337273414 1102 66-67 0.5734458734855785 1102 68-69 0.5892878735272369 1102 70-71 0.1919102212438446 1102 72-73 0.48526512887744744 1102 74-75 0.5399547342077327 1102 76-77 0.4313936235969038 1102 78-79 0.40580092714128924 1102 80-81 0.4702520044096907 1102 82-83 0.38014725551785133 1102 84-85 0.6144480284430784 1102 86-87 0.36617734781886924 1102 88-89 0.6881550345123877 1102 90-91 0.6709995254322543 1102 92-93 0.8467676364028378 1102 94-95 1.1842160803429636 1102 96-97 1.1309845275515151 1102 98-99 0.7105356035466066 1102 100-101 0.29169255352795354 1103 1 0.2914462553166146 1103 2 0.27314101389418965 1103 3 0.25634147472859325 1103 4 0.19720069883257452 1103 5 0.23349680482282764 1103 6 0.18831831572636304 1103 7 0.3183901673472036 1103 8 0.29350857194916813 1103 9 0.13237336894860618 1103 10-11 0.22062127974160006 1103 12-13 0.19949385341730874 1103 14-15 0.20033203940755584 1103 16-17 0.19710891419401122 1103 18-19 0.22028872890419393 1103 20-21 0.1339423218804896 1103 22-23 0.2926304186467732 1103 24-25 0.22598250864573544 1103 26-27 0.11753002762334575 1103 28-29 0.20356354680911437 1103 30-31 0.007065097278832866 1103 32-33 -0.17544182817927378 1103 34-35 -0.22068703171369464 1103 36-37 -0.1473636172045545 1103 38-39 0.01930891144048985 1103 40-41 0.1252473395292597 1103 42-43 0.07286774823731434 1103 44-45 -0.3067297752281348 1103 46-47 -0.4468284351472107 1103 48-49 -0.17309472203061915 1103 50-51 -0.1609182285818207 1103 52-53 -0.23089160401977438 1103 54-55 -0.38362467141084977 1103 56-57 -0.39037189883554646 1103 58-59 -0.5566845592727319 1103 60-61 -0.2771872269575546 1103 62-63 -0.4358580680097326 1103 64-65 -0.49455592656573444 1103 66-67 -0.4833191378292767 1103 68-69 -0.5044323234289401 1103 70-71 -0.5225504216659438 1103 72-73 -0.6698873096443911 1103 74-75 -0.4471962537521321 1103 76-77 -0.386985891453854 1103 78-79 -0.7270597176523488 1103 80-81 -0.6561258528559542 1103 82-83 -0.6368933154760903 1103 84-85 -0.43750257709589846 1103 86-87 -0.4494588154447037 1103 88-89 -0.37031368068059933 1103 90-91 -0.5356878949457844 1103 92-93 -0.2627556797325461 1103 94-95 -0.2820771922049161 1103 96-97 -0.14487774441472823 1103 98-99 -0.3163680824349875 1103 100-101 -0.6520805088768249 1104 1 0.28735426154760546 1104 2 0.35587495609478026 1104 3 0.5008021706634409 1104 4 0.41792740818053886 1104 5 0.5360211168880795 1104 6 0.5093622814094161 1104 7 0.426715979669261 1104 8 0.5471804811305745 1104 9 0.497790948880926 1104 10-11 0.6223077476281063 1104 12-13 0.4493094389253969 1104 14-15 0.4029491399678875 1104 16-17 0.5538912459118208 1104 18-19 0.46293592760051183 1104 20-21 0.44322814637707353 1104 22-23 0.5095325088185945 1104 24-25 0.6685403006600055 1104 26-27 0.6215099988356627 1104 28-29 0.4868823313009685 1104 30-31 0.5700946944837639 1104 32-33 0.581817973266034 1104 34-35 0.6169035110461607 1104 36-37 0.7991350438088531 1104 38-39 0.5706797082167725 1104 40-41 0.4673631708528845 1104 42-43 0.3716530432687932 1104 44-45 0.4903836359855305 1104 46-47 0.14028918219641895 1104 48-49 0.11567934960886106 1104 50-51 0.3152981144523821 1104 52-53 0.39414459194783547 1104 54-55 0.2880985102833904 1104 56-57 0.2255598223593367 1104 58-59 0.29612097168248397 1104 60-61 0.45317130863285726 1104 62-63 0.5761949250004825 1104 64-65 0.18416232402238464 1104 66-67 0.3701469034837963 1104 68-69 0.6982323338771472 1104 70-71 0.3129254311321894 1104 72-73 0.2590893325957211 1104 74-75 0.25236453328030706 1104 76-77 -0.04091545251565876 1104 78-79 -0.09074094795592558 1104 80-81 -0.4430029387476644 1104 82-83 -0.7275787455687315 1104 84-85 -0.39440242681658333 1104 86-87 -0.04493373824425717 1104 88-89 0.15458597458583156 1104 90-91 0.13641151344829439 1104 92-93 0.08706223077200193 1104 94-95 0.29602577977465216 1104 96-97 -0.05858338718113387 1104 98-99 0.17735654075706364 1104 100-101 -0.9970518585795407 1105 1 0.15474280739010027 1105 2 0.1875359382491837 1105 3 0.161859085832198 1105 4 0.06700643637140757 1105 5 0.20759303270341434 1105 6 0.12830463883861398 1105 7 0.30720382397289114 1105 8 0.3024703104335629 1105 9 0.3317836586960041 1105 10-11 0.3532184953974067 1105 12-13 0.27580687685375693 1105 14-15 0.3301442776893424 1105 16-17 0.3205396601753705 1105 18-19 0.3301850547180578 1105 20-21 0.2805931728868387 1105 22-23 0.2873485650063614 1105 24-25 0.2672780560589487 1105 26-27 0.3917125803046986 1105 28-29 0.053699260371182334 1105 30-31 0.09182380996288231 1105 32-33 0.010399215992009658 1105 34-35 0.22621688392860761 1105 36-37 0.4462228089064908 1105 38-39 0.20576837044359308 1105 40-41 0.5532885474854936 1105 42-43 0.47175164665686964 1105 44-45 0.3007509336061105 1105 46-47 0.36849136763544976 1105 48-49 0.5267989021757842 1105 50-51 0.7223290613681925 1105 52-53 0.7329300730030539 1105 54-55 0.6525430984121954 1105 56-57 0.5854803738917269 1105 58-59 0.9483197464141497 1105 60-61 1.0801140782565462 1105 62-63 0.7959208560750959 1105 64-65 0.9597436394885683 1105 66-67 0.8655248696676843 1105 68-69 0.6946761461104352 1105 70-71 0.6990890319339478 1105 72-73 0.832938825030741 1105 74-75 0.795087279950419 1105 76-77 0.67075147008876 1105 78-79 0.6993510279913124 1105 80-81 0.7689256465361858 1105 82-83 1.2157909848075796 1105 84-85 1.0472882171766926 1105 86-87 0.8758875390737337 1105 88-89 0.9092383168204101 1105 90-91 0.9844855144828237 1105 92-93 1.1238485685451813 1105 94-95 1.189660405728354 1105 96-97 0.9009609358500263 1105 98-99 0.5156035422441967 1105 100-101 -0.24324815206201755 1106 1 -0.027966167956584798 1106 2 0.013157086169059085 1106 3 -0.055037100608480216 1106 4 -0.13582965761934673 1106 5 -0.13497726775883478 1106 6 -0.11731362002117152 1106 7 -0.15206042872356562 1106 8 -0.28193839526751674 1106 9 -0.09399157242880563 1106 10-11 -0.10851349612801187 1106 12-13 0.0338601318614522 1106 14-15 -0.12902367300403483 1106 16-17 -0.48679614490937695 1106 18-19 -0.167022187192579 1106 20-21 -0.016354053615479813 1106 22-23 0.04966318365043065 1106 24-25 0.05434228949500408 1106 26-27 -0.044932739417959056 1106 28-29 -0.14657279278751645 1106 30-31 0.06538891011696535 1106 32-33 -0.049837686935568115 1106 34-35 -0.11908598587108088 1106 36-37 -0.14106291913665814 1106 38-39 -0.05802689149786033 1106 40-41 -0.305424376243316 1106 42-43 -0.5187144550380367 1106 44-45 -0.3554148021411834 1106 46-47 -0.22155100524590665 1106 48-49 -0.3105470146192886 1106 50-51 -0.4600308192173159 1106 52-53 -0.21566873285210875 1106 54-55 -0.08821190929197087 1106 56-57 -0.4050098033039582 1106 58-59 -0.29785224896335194 1106 60-61 -0.07203104886209744 1106 62-63 -0.019492279518122047 1106 64-65 -0.1047425893249887 1106 66-67 -0.4253673683908694 1106 68-69 -0.2783592930266998 1106 70-71 -0.5089473702232183 1106 72-73 -0.3733641410709545 1106 74-75 -0.27328714223756023 1106 76-77 -0.8617624937016828 1106 78-79 -0.9499652277868087 1106 80-81 -0.7421972348197485 1106 82-83 -0.7424380213557491 1106 84-85 -0.48039860870928663 1106 86-87 -0.20200874366894794 1106 88-89 -0.4692405930409116 1106 90-91 -0.61764275978528 1106 92-93 -0.43600312637007477 1106 94-95 -0.36639939781555597 1106 96-97 -0.5301349809450393 1106 98-99 -0.8415926249360801 1106 100-101 -1.2337262915073186 1107 1 -0.08163578112664638 1107 2 -0.040319922994839885 1107 3 0.07434413367909087 1107 4 0.10245799139533318 1107 5 0.09238669299049462 1107 6 0.15662401682904203 1107 7 0.0875267904800694 1107 8 0.14358873148619722 1107 9 0.12865865869600412 1107 10-11 0.14926365089980465 1107 12-13 0.14034784575327564 1107 14-15 0.07398399060799932 1107 16-17 0.2789801506059959 1107 18-19 0.19645299730179033 1107 20-21 0.23833838819783892 1107 22-23 0.39993080184846264 1107 24-25 0.4404464173029723 1107 26-27 0.3724168267161758 1107 28-29 0.12402671252429798 1107 30-31 0.03383936020211564 1107 32-33 0.035907589197755385 1107 34-35 0.11036012555540964 1107 36-37 0.10286904096390259 1107 38-39 -0.07821787357553944 1107 40-41 0.273302004423293 1107 42-43 0.07133298636979646 1107 44-45 -0.4103809443843147 1107 46-47 -0.20274068978081772 1107 48-49 -0.18576090643665566 1107 50-51 -0.4408363094452028 1107 52-53 -0.2916662188629786 1107 54-55 -0.34488513125288023 1107 56-57 -0.4555034777829121 1107 58-59 -0.55594161722221 1107 60-61 -0.4560925604994317 1107 62-63 -0.4564864405756204 1107 64-65 -0.5572494825209979 1107 66-67 -0.3363964820069576 1107 68-69 -0.26979753810008944 1107 70-71 -0.4456627623244245 1107 72-73 -0.1258679931510791 1107 74-75 -0.20832181095867242 1107 76-77 -0.42871772847583145 1107 78-79 -0.6041477758364415 1107 80-81 -0.2340946883920445 1107 82-83 -0.3801569816995958 1107 84-85 -0.6132680029189999 1107 86-87 -0.3729387169071323 1107 88-89 -0.3662177238494415 1107 90-91 -0.0569917582444468 1107 92-93 -0.13180334533041815 1107 94-95 -0.4161186014486802 1107 96-97 -0.7946431311356186 1107 98-99 -0.6322807280907305 1107 100-101 -0.6054266807701527 1108 1 -0.10749591452161411 1108 2 -0.06320013167211158 1108 3 0.007831553609094044 1108 4 0.17257454689952567 1108 5 -0.07509167985504206 1108 6 -0.10528082369133074 1108 7 0.06180203316645816 1108 8 0.05164691018261891 1108 9 0.1531254090096823 1108 10-11 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-0.3329574945577036 2316 78-79 -0.3976565477662639 2316 80-81 -0.13694556558502669 2316 82-83 -0.3630663384247441 2316 84-85 -0.5275223888839164 2316 86-87 -0.14453228415858987 2316 88-89 -0.3018317589371655 2316 90-91 -0.2106028693555615 2316 92-93 -0.13424486579825512 2316 94-95 -0.45186421548376643 2316 96-97 -0.326046639907549 2316 98-99 -0.5545321900790334 2316 100-101 -0.7375758035771707 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4928.0 3 817.0 4 668.0 5 602.0 6 658.0 7 770.0 8 952.0 9 1197.0 10 1621.0 11 2536.0 12 4046.0 13 5966.0 14 7285.0 15 10708.0 16 14228.0 17 20686.0 18 18729.0 19 10264.0 20 8309.0 21 7251.0 22 7263.0 23 7489.0 24 7937.0 25 8950.0 26 10076.0 27 11675.0 28 13448.0 29 16154.0 30 18929.0 31 23008.0 32 28165.0 33 35873.0 34 47145.0 35 66277.0 36 90304.0 37 125701.0 38 84522.0 39 5494.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.67173589741832 19.703933165653506 15.31611302625537 19.3082179106728 2 15.921944539541252 21.989729544676482 40.87422115713797 21.2141047586443 3 18.99733507339515 27.992147788275417 31.663550274443576 21.346966863885857 4 11.331170655559987 19.42175094668547 38.145085801294044 31.101992596460498 5 11.155286147263327 39.7137841655581 34.16747809152872 14.963451595649849 6 27.42994839132623 38.24157467485713 18.389858751599487 15.938618182217157 7 24.462208963572376 37.51739398068874 20.721149298277123 17.29924775746176 8 25.816666278609514 33.97079710135002 20.137837900766026 20.074698719274434 9 27.34484195063664 15.891675756263602 21.200046041777433 35.56343625132234 10-11 23.824894863070238 28.191973628595875 26.65179210597885 21.33133940235504 12-13 26.335232257215544 27.39398741117642 27.218670497412056 19.05210983419598 14-15 23.00536562300845 27.378451164607448 29.139581577342405 20.4766016350417 16-17 18.62622087393776 30.43905206800781 29.8793328047705 21.05539425328393 18-19 17.89522662841957 27.16998690452777 35.726723329196595 19.20806313785606 20-21 20.920133654220415 25.649974508116287 35.83932712391248 17.590564713750815 22-23 19.575976916599696 26.802910658793007 35.27384109865576 18.347271325951546 24-25 18.544586127053453 26.77197756976527 36.287543757613 18.395892545568273 26-27 18.43003108876473 27.0702540286543 35.93452717114173 18.56518771143924 28-29 18.022272593965074 27.376022014948898 35.955260207815414 18.646445183270615 30-31 18.945975994963614 27.063448732237873 35.95419026330734 18.036385009491177 32-33 17.976511055837634 27.268827813979623 36.118499941757285 18.63616118842546 34-35 18.462990654589717 27.628383798183524 35.7589523934128 18.149673153813957 36-37 18.712353162779547 27.417514298434874 35.095311483269974 18.774821055515602 38-39 18.28534415882747 27.697649371483664 35.284556074174446 18.732450395514423 40-41 19.22397176087128 27.238570650890004 34.965505671449655 18.571951916789065 42-43 18.510189701488756 27.5175694543831 34.702984975488704 19.26925586863944 44-45 19.230885089404424 27.79992140571493 34.00537836849766 18.96381513638299 46-47 19.143097371066116 27.85275888795467 33.10699780077726 19.89714594020196 48-49 19.203676204286214 27.85942023925178 32.88572040828762 20.051183148174392 50-51 19.576165288690323 28.551738304318803 31.532412400075316 20.339684006915558 52-53 19.556903320315843 28.87220155320001 31.31707116722741 20.253823959256735 54-55 19.848571680932398 28.562478560321754 31.136403940313308 20.452545818432544 56-57 19.917090557619392 28.989828670703982 30.542600955183584 20.550479816493038 58-59 19.946840378033105 28.833275020249815 30.808547990526574 20.411336611190496 60-61 20.057342291583087 28.567154696851237 30.824836277351576 20.550666734214097 62-63 19.60796127951776 28.305280520143867 31.193226517422918 20.893531682915455 64-65 19.682760717043752 28.50347513185784 30.83310056248391 20.980663588614494 66-67 19.573333077743037 28.656555788401356 30.693633268545756 21.076477865309847 68-69 20.072081227311976 28.22864115998321 30.63876731925081 21.060510293454 70-71 19.862637118104555 28.122759773805196 30.67885144309081 21.335751664999442 72-73 19.89348947938156 27.978388415577264 30.863881389621316 21.264240715419856 74-75 20.08467428333571 28.13427665377748 30.33150858244515 21.449540480441666 76-77 19.650855884962052 28.219425248332584 30.57591529860145 21.55380356810391 78-79 19.804356962638764 28.24639988609964 30.134038274354758 21.815204876906837 80-81 19.866380992148514 28.282872534859365 29.78848220580887 22.062264267183256 82-83 20.05120026284627 28.542024600768002 29.791776471151937 21.614998665233788 84-85 20.360231109736056 28.16179324289452 29.632136875227168 21.845838772142258 86-87 20.12844154759607 28.23564684187397 29.599739338406927 22.036172272123036 88-89 20.762390367095612 27.947625464964037 29.504330533962232 21.785653633978118 90-91 20.86115059859215 28.077688526555566 29.266103755973077 21.795057118879203 92-93 20.854901165792995 27.923283122917127 29.499932197446515 21.721883513843363 94-95 20.945216662374875 28.082624274733043 29.118132906711082 21.854026156181 96-97 21.28827083517373 27.767973742076023 28.9206955090212 22.02305991372905 98-99 21.55651476102181 27.464210102515086 28.710385985862064 22.26888915060104 100-101 22.33385594544368 26.837136889653923 28.752665595860034 22.07634156904236 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3112.0 1 1769.5 2 553.0 3 660.5 4 460.5 5 372.5 6 706.5 7 1325.0 8 1913.5 9 2149.5 10 1831.5 11 1433.0 12 1468.5 13 1650.0 14 1819.0 15 1974.0 16 2196.0 17 2425.5 18 2734.5 19 3153.0 20 3461.0 21 3908.0 22 4429.5 23 4940.0 24 5632.5 25 6465.0 26 7763.0 27 9353.0 28 10543.5 29 11753.0 30 13180.5 31 14665.5 32 16112.5 33 17721.0 34 19925.5 35 22036.5 36 23994.0 37 25293.5 38 24902.5 39 23913.0 40 23703.5 41 23779.5 42 23892.0 43 24174.0 44 24250.0 45 24372.0 46 24834.5 47 25395.0 48 25852.0 49 26169.5 50 26240.5 51 24616.0 52 21563.5 53 19012.0 54 16937.0 55 15014.5 56 13138.0 57 11008.0 58 8754.0 59 6850.5 60 5142.0 61 3708.0 62 2671.5 63 1868.0 64 1320.5 65 979.5 66 753.5 67 567.5 68 423.0 69 315.5 70 229.0 71 186.5 72 175.5 73 165.0 74 137.0 75 93.5 76 57.5 77 37.5 78 30.5 79 25.5 80 14.5 81 8.5 82 5.5 83 3.5 84 4.5 85 3.5 86 2.0 87 1.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14603795070569042 2 0.052283502501943524 3 0.15821918558179768 4 0.13426731925237329 5 0.2001007347063912 6 0.2040699011266958 7 0.1660206506148102 8 0.06829703599075869 9 0.11770631453317129 10-11 0.10271928960133145 12-13 0.15000711712599502 14-15 0.13508852609795355 16-17 0.13419888534857494 18-19 0.13556756342454204 20-21 0.13522539390555027 22-23 0.19845832101523064 24-25 0.22117837707628465 26-27 0.15164953081715557 28-29 0.1294769459864884 30-31 0.10176121494815447 32-33 0.12687645764215089 34-35 0.09601276702909263 36-37 0.08992214959103899 38-39 0.05207820079054846 40-41 0.06015340143875439 42-43 0.06275388978309189 44-45 0.040923474471416525 46-47 0.05118856004116983 48-49 0.042908057681568836 50-51 0.053994350096902406 52-53 0.02798946665352736 54-55 0.05276253982853201 56-57 0.02689452419275367 58-59 0.05180446517535504 60-61 0.038459853934675735 62-63 0.05098325832977477 64-65 0.041060342279013236 66-67 0.04058130495242475 68-69 0.06617558497300967 70-71 0.05153072956016161 72-73 0.051393861752564905 74-75 0.041607813509400085 76-77 0.021898849215473727 78-79 0.023130659483844126 80-81 0.02696295809655203 82-83 0.0228569238686507 84-85 0.02141981188888524 86-87 0.025046808790198074 88-89 0.06405413395526065 90-91 0.10874147313558673 92-93 0.07815151813772186 94-95 0.07609850102377119 96-97 0.06713365962618664 98-99 0.05447338742349089 100-101 0.13194056652322922 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 730632.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.245760948651544 #Duplication Level Percentage of deduplicated Percentage of total 1 78.71269045882899 48.99531313925475 2 12.69602259967707 15.805471754763529 3 4.202474961537978 7.8475875554575945 4 1.8273662703974298 4.549832161311493 5 0.9185900333000473 2.85891678113043 6 0.5042948785570093 1.8834131074973313 7 0.30128442053519616 1.3127574612730775 8 0.19943918898892932 0.9931395265278266 9 0.1241120245019215 0.6952902667199811 >10 0.44963558605419984 4.789098946047448 >50 0.0358991766914673 1.5883234381772797 >100 0.024666600814543967 3.002101330328749 >500 0.002202375072010041 1.0320023202300916 >1k 6.607125216030122E-4 0.9624984445185274 >5k 4.4047501440200814E-4 2.172441653651204 >10k+ 2.2023750720100407E-4 1.5118121131107134 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11028 1.5093781821765266 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9871 1.3510221287871322 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5976 0.8179220181979437 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3287 0.4498844835703884 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2134 0.29207590141138084 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1600 0.21898849215473726 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 958 0.13111935967764893 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 904 0.12372849806742654 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 834 0.1141477515356568 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 804 0.11004171730775547 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 774 0.10593568307985415 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 754 0.10319832692791994 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 731 0.1000503673531956 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.368678075967108E-4 0.0 5 0.0 0.0 0.0 1.368678075967108E-4 0.0 6 0.0 0.0 0.0 1.368678075967108E-4 0.0 7 0.0 0.0 0.0 1.368678075967108E-4 0.0 8 0.0 0.0 0.0 1.368678075967108E-4 0.0 9 0.0 0.0 0.0 1.368678075967108E-4 0.0 10-11 0.0 0.0 0.0 2.053017113950662E-4 0.0 12-13 0.0 0.0 0.0 2.737356151934216E-4 0.0 14-15 0.0 0.0 0.0 2.737356151934216E-4 0.0 16-17 0.0 0.0 0.0 5.474712303868432E-4 0.0 18-19 0.0 0.0 0.0 6.84339037983554E-4 0.0 20-21 0.0 0.0 0.0 0.001026508556975331 0.0 22-23 0.0 0.0 0.0 0.002805790055732571 0.0 24-25 0.0 0.0 0.0 0.008280502359601004 0.0 26-27 0.0 0.0 0.0 0.017861248891370758 0.0 28-29 0.0 0.0 0.0 0.041813115220795144 0.0 30-31 0.0 0.0 0.0 0.10716749334822454 0.0 32-33 0.0 0.0 0.0 0.215087759638231 0.0 34-35 0.0 0.0 0.0 0.37570213185297113 0.0 36-37 0.0 0.0 0.0 0.5970173767368525 0.0 38-39 0.0 0.0 0.0 0.9005901739863571 0.0 40-41 0.0 0.0 0.0 1.2281832714690843 0.0 42-43 0.0 0.0 0.0 1.5174533828247325 0.0 44-45 0.0 0.0 0.0 1.8574330168949622 0.0 46-47 0.0 0.0 0.0 2.2280710398668546 0.0 48-49 0.0 0.0 0.0 2.6057577549299786 0.0 50-51 0.0 0.0 0.0 2.9952151014464192 0.0 52-53 0.0 0.0 0.0 3.3455282549902003 0.0 54-55 0.0 0.0 0.0 3.7163715796734884 0.0 56-57 0.0 0.0 0.0 4.133270921613069 0.0 58-59 0.0 0.0 0.0 4.574258997689672 0.0 60-61 0.0 0.0 0.0 5.027428308642381 0.0 62-63 0.0 0.0 0.0 5.469853496698748 0.0 64-65 0.0 0.0 0.0 5.905572162182877 0.0 66-67 0.0 0.0 0.0 6.385499129520744 0.0 68-69 0.0 0.0 0.0 6.92161033187706 0.0 70-71 0.0 0.0 0.0 7.489269563884418 0.0 72-73 0.0 0.0 0.0 8.045431900053652 0.0 74-75 0.0 0.0 0.0 8.582980214389734 0.0 76-77 0.0 0.0 0.0 9.143111716979273 0.0 78-79 0.0 0.0 0.0 9.731232686222338 0.0 80-81 0.0 0.0 0.0 10.368762934007819 0.0 82-83 0.0 0.0 0.0 11.003076788314775 0.0 84-85 0.0 0.0 0.0 11.618708186884778 0.0 86-87 0.0 0.0 0.0 12.231807531014244 0.0 88-89 0.0 0.0 0.0 12.886035651326523 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5340 0.0 48.591103 1 GTATCAA 8785 0.0 38.4243 1 GCGGTAT 75 3.0357437E-4 31.740242 1 CGGTATC 60 0.003930401 31.698883 2 TATCAAC 11030 0.0 30.434372 2 ATCAACG 11175 0.0 30.082026 3 TCAACGC 11275 0.0 29.899563 4 CAACGCA 11370 0.0 29.566105 5 AACGCAG 11540 0.0 29.118574 6 CGGATCA 35 0.008388566 27.145334 94-95 ATAGCCC 575 0.0 26.461672 3 TAGCCCT 1075 0.0 26.096287 4 GTATAGC 570 0.0 25.893354 1 ACGCAGA 13045 0.0 25.686298 7 ACGAAGG 185 0.0 25.678019 94-95 CGCAGAG 12910 0.0 25.658619 8 GCAGAGT 13030 0.0 24.54694 9 GTAGGAC 525 0.0 24.45342 3 GAGTACT 8010 0.0 24.151724 12-13 GACGTGG 280 1.8189894E-12 23.76438 7 >>END_MODULE