FastQCFastQC Report
Thu 26 May 2016
SRR938016_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938016_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730632
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105471.4435447667225088No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95911.3126991426600532No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59260.8110786278181082No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31220.4273012953169311No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT23720.324650439619398No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22610.3094581129761631No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16110.2204940380383011No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT14170.19394168336453918RNA PCR Primer, Index 26 (100% over 22bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9330.12769766448773118No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8240.1127790734596897No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT7790.1066200221178377RNA PCR Primer, Index 26 (100% over 24bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7700.10538821184946731No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7530.10306145912032322No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7410.1014190454291627No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7400.10128217762156598No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53000.048.8473321
CATTCCG1850.035.935859
GTATCAA86350.035.647811
TCGTATC501.3009776E-633.2452194-95
TATCAAC108250.028.2145772
ATCAACG111850.027.2602633
TCAACGC112500.027.0586934
CAACGCA113800.026.7495865
AACGCAG115800.026.285796
CTCCGAC1600.023.74657694-95
GAGTACT81500.023.27719912-13
CGCAGAG130900.023.0722038
AGTACTT80100.023.00227512-13
ACGCAGA131950.022.9965697
TAGGCAT2903.6379788E-1222.926165
GTCCTAC4450.022.4171031
GTACTTT84750.022.27250314-15
GCAGAGT134250.022.0012669
CGGACCA550.00287557121.59370870-71
GCATTCC3351.8189894E-1221.2626828
Produced by FastQC (version 0.11.5)