FastQCFastQC Report
Thu 26 May 2016
SRR938015_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938015_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2533837
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108430.4279280790358653No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89410.3528640555805287No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54170.21378644324792795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45750.055.670021
GTATCAA88050.045.0135151
ATCAACG109300.035.610633
TATCAAC111700.034.802952
TCAACGC112100.034.7635544
CAACGCA113150.034.3989565
AACGCAG115300.033.8339426
ACGCAGA134950.028.7239767
CGCAGAG136650.028.4013968
GTGGTAT20150.027.220371
GTACATG101100.026.3241441
GCAGAGT148400.025.7674889
TACATGG102650.024.769132
TGGTATC21550.023.8172932
ACATGGG103400.023.8081233
GAGTACT89650.022.7053312-13
GTACTTT93550.021.70841814-15
CAGAGTA145100.021.427410-11
AGTACTT91500.021.13005312-13
AGAGTAC140400.020.52064310-11
Produced by FastQC (version 0.11.5)