FastQCFastQC Report
Thu 26 May 2016
SRR938015_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938015_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2533837
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109570.43242718454265217No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89970.3550741424961432No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58030.22902025663055675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44450.059.7034231
GTATCAA84350.041.3290751
ATCAACG105400.032.7075773
TCAACGC107500.032.023144
TATCAAC108500.031.9108562
CAACGCA108800.031.6842145
AACGCAG112100.030.8022526
GTGGTAT18850.030.0242671
TGGTATC19100.028.8842722
ACGCAGA129200.026.6151987
GTACATG97300.026.4925171
CGCAGAG130450.026.2873468
TACATGG98950.025.2817272
ACATGGG99700.024.2758033
GCAGAGT143800.023.4835839
GGACCGA5950.023.1491286
GTGTAGC14000.022.7605931
GAGTACT90700.021.5484612-13
CAGAGTA141000.021.17092310-11
AGAGTAC135400.020.73110610-11
Produced by FastQC (version 0.11.5)