##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938015_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2533837 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.51741252495721 34.0 31.0 34.0 31.0 34.0 2 32.743052927240385 34.0 31.0 34.0 31.0 34.0 3 32.80879906639614 34.0 31.0 34.0 31.0 34.0 4 36.251738371489566 37.0 35.0 37.0 35.0 37.0 5 36.17945511096412 37.0 37.0 37.0 35.0 37.0 6 36.232648351097566 37.0 37.0 37.0 35.0 37.0 7 36.20097780559681 37.0 37.0 37.0 35.0 37.0 8 36.20158479018185 37.0 37.0 37.0 35.0 37.0 9 37.900895756120065 39.0 38.0 39.0 35.0 39.0 10-11 37.8755561229866 39.0 38.0 39.0 35.0 39.0 12-13 37.789438310356985 39.0 38.0 39.0 35.0 39.0 14-15 39.060349383168685 40.0 38.0 41.0 36.0 41.0 16-17 38.967575065010095 40.0 38.0 41.0 36.0 41.0 18-19 39.207726069198614 40.5 39.0 41.0 36.0 41.0 20-21 39.18269545357495 40.0 39.0 41.0 36.0 41.0 22-23 39.076996468202175 40.0 39.0 41.0 35.5 41.0 24-25 38.97009929999444 40.0 38.0 41.0 35.0 41.0 26-27 38.822481280366496 40.0 38.0 41.0 35.0 41.0 28-29 38.700532828275854 40.0 38.0 41.0 34.5 41.0 30-31 38.47890689101153 40.0 38.0 41.0 34.0 41.0 32-33 38.279757932337404 40.0 38.0 41.0 34.0 41.0 34-35 38.18701656815335 40.0 38.0 41.0 34.0 41.0 36-37 38.13838715750066 40.0 38.0 41.0 34.0 41.0 38-39 37.966078125783156 40.0 38.0 41.0 33.0 41.0 40-41 37.76335119425598 40.0 37.0 41.0 33.0 41.0 42-43 37.520593274153 40.0 37.0 41.0 33.0 41.0 44-45 37.257828739575594 40.0 37.0 41.0 32.0 41.0 46-47 36.90594915931845 40.0 36.0 41.0 31.0 41.0 48-49 36.51376292160861 39.0 36.0 41.0 30.0 41.0 50-51 36.148874019915255 39.0 35.0 40.5 30.0 41.0 52-53 35.6345325291248 38.5 35.0 40.0 28.0 41.0 54-55 35.26167784273416 38.0 34.5 40.0 27.5 41.0 56-57 34.89295088831681 38.0 34.0 40.0 26.5 41.0 58-59 34.38699292811653 37.5 33.5 40.0 25.5 41.0 60-61 33.91262520043712 37.0 33.0 40.0 24.5 41.0 62-63 33.35867007230536 36.5 32.5 39.0 22.5 40.5 64-65 32.78925084762753 36.0 32.0 39.0 21.5 40.0 66-67 32.22981056003208 35.0 31.0 38.5 19.0 40.0 68-69 31.608552562773376 35.0 31.0 38.0 16.0 40.0 70-71 30.98914689461082 35.0 30.0 37.5 10.5 39.5 72-73 30.316578966997483 34.0 29.0 36.5 7.5 39.0 74-75 29.664251883605772 34.0 29.0 36.0 4.5 39.0 76-77 28.340230843578336 32.5 27.0 35.0 2.0 37.0 78-79 29.00017522831974 34.0 29.0 35.0 2.0 37.0 80-81 28.71449446037768 34.0 28.0 35.0 2.0 37.0 82-83 28.16167752700746 33.5 27.0 35.0 2.0 36.5 84-85 27.51097387085278 33.0 26.0 35.0 2.0 36.0 86-87 26.7939066325103 32.5 25.0 35.0 2.0 35.5 88-89 26.000220614033182 32.0 23.5 34.5 2.0 35.0 90-91 25.349073756520248 32.0 20.5 34.0 2.0 35.0 92-93 24.406836548680914 31.0 15.0 34.0 2.0 35.0 94-95 23.507140948687702 31.0 4.5 34.0 2.0 35.0 96-97 22.45826783648672 30.0 2.0 34.0 2.0 35.0 98-99 21.038576277795293 29.5 2.0 34.0 2.0 35.0 100-101 17.116580111506778 22.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1392793232564813 1101 2 0.13729745855435738 1101 3 0.1553885880307817 1101 4 0.11194984972603805 1101 5 0.04257689571017664 1101 6 0.0840474314973747 1101 7 0.15373152477507546 1101 8 0.15436465316918913 1101 9 0.08069846345401999 1101 10-11 0.22655709803522228 1101 12-13 0.23575535279340443 1101 14-15 0.41737922294176855 1101 16-17 0.43266562614677184 1101 18-19 0.3350131499551381 1101 20-21 0.30025661870948994 1101 22-23 0.3336689638956827 1101 24-25 0.34246974716964473 1101 26-27 0.40230776358091447 1101 28-29 0.452783622279064 1101 30-31 0.46259626752832617 1101 32-33 0.41523516314433806 1101 34-35 0.45997042266574795 1101 36-37 0.3974646645443727 1101 38-39 0.49928340991368003 1101 40-41 0.5973588407405472 1101 42-43 0.6884811011737639 1101 44-45 0.7039234833215176 1101 46-47 0.7965670188969369 1101 48-49 0.862237316565313 1101 50-51 0.8200261099294792 1101 52-53 0.893214801103305 1101 54-55 0.9765436273541681 1101 56-57 1.0544025992853179 1101 58-59 1.2007043224910774 1101 60-61 1.1749304054696452 1101 62-63 1.2135628887014178 1101 64-65 1.3354035566242572 1101 66-67 1.6968381609690013 1101 68-69 1.9140436043294038 1101 70-71 1.970582367259727 1101 72-73 1.9791322291955566 1101 74-75 2.0158499481290377 1101 76-77 1.9939869628964892 1101 78-79 2.0247031918134617 1101 80-81 2.1588191639618017 1101 82-83 2.3164092446708544 1101 84-85 2.5556320305308304 1101 86-87 2.727695799358983 1101 88-89 3.0183311770600874 1101 90-91 3.382650481121672 1101 92-93 3.439224614277027 1101 94-95 3.7343132644357233 1101 96-97 4.1425724097615095 1101 98-99 4.366673094391878 1101 100-101 3.9530622454898996 1102 1 0.1876549201076827 1102 2 0.143413973588558 1102 3 0.18597640581371877 1102 4 0.139715125890703 1102 5 -0.008311122098568546 1102 6 0.10076128167159482 1102 7 0.0972738366862913 1102 8 0.17959290842793507 1102 9 0.19365537724934256 1102 10-11 0.18325856757967784 1102 12-13 0.31943792473816757 1102 14-15 0.4254594994290457 1102 16-17 0.43987240282763196 1102 18-19 0.3801062668089301 1102 20-21 0.25291684099622813 1102 22-23 0.37683985691413824 1102 24-25 0.4347326601261514 1102 26-27 0.4453671502759775 1102 28-29 0.3665741113744261 1102 30-31 0.44370915306193837 1102 32-33 0.4883070917802925 1102 34-35 0.5098399622837633 1102 36-37 0.5257433134022946 1102 38-39 0.6163866248446439 1102 40-41 0.6772461649114163 1102 42-43 0.7330318093296597 1102 44-45 0.6919406871542648 1102 46-47 0.7165798834601063 1102 48-49 0.8550767151973986 1102 50-51 0.9476312847907522 1102 52-53 0.8977881734739341 1102 54-55 1.066526004112454 1102 56-57 1.1484151331606611 1102 58-59 1.3173377187357573 1102 60-61 1.1359074266981892 1102 62-63 1.2839433410178458 1102 64-65 1.3051444102828 1102 66-67 1.6268035194023511 1102 68-69 1.7154762488641815 1102 70-71 1.7778798814551102 1102 72-73 1.9878208503079549 1102 74-75 2.048908850890683 1102 76-77 2.09314673351205 1102 78-79 2.2018972249145072 1102 80-81 2.3631293975403302 1102 82-83 2.3361861916962745 1102 84-85 2.7377716614509495 1102 86-87 2.7524869014366686 1102 88-89 3.007032340113387 1102 90-91 3.0996466257854998 1102 92-93 3.2446662438421363 1102 94-95 3.3855567716624613 1102 96-97 3.6642064512507346 1102 98-99 4.240613885139069 1102 100-101 4.042591942870207 1103 1 0.12695235680665462 1103 2 0.10989080228502246 1103 3 0.12099516167492652 1103 4 0.05515452101418106 1103 5 -0.011845429694695042 1103 6 -0.023339023111155655 1103 7 0.024695491891044696 1103 8 0.036679107240061626 1103 9 0.10085782332624404 1103 10-11 0.16084091753278784 1103 12-13 0.18154522389415462 1103 14-15 0.25776109758472643 1103 16-17 0.22056607663193972 1103 18-19 0.17327454205118897 1103 20-21 0.11355541945824399 1103 22-23 0.22848294850526685 1103 24-25 0.2141915730677013 1103 26-27 0.2771969212981702 1103 28-29 0.3358965558883398 1103 30-31 0.37217703364962773 1103 32-33 0.3734889845592875 1103 34-35 0.4027620668476928 1103 36-37 0.4123348263750941 1103 38-39 0.4692395671703693 1103 40-41 0.5855641939829965 1103 42-43 0.5760310864475073 1103 44-45 0.5907650267916509 1103 46-47 0.7587761752700501 1103 48-49 0.7404510509273123 1103 50-51 0.8649062144562762 1103 52-53 0.8970247513107594 1103 54-55 1.057805993953032 1103 56-57 1.1501314898346209 1103 58-59 1.270791434740758 1103 60-61 1.3631947625844063 1103 62-63 1.4541420359225015 1103 64-65 1.5617978566722925 1103 66-67 1.5868598846727409 1103 68-69 1.746914295910134 1103 70-71 1.846269905681428 1103 72-73 1.9783383948531252 1103 74-75 2.008522695060737 1103 76-77 1.987866274774948 1103 78-79 1.9182133588626122 1103 80-81 2.1644579377091304 1103 82-83 2.2590590621245354 1103 84-85 2.5215634713915556 1103 86-87 2.781740183712383 1103 88-89 2.9559348291724667 1103 90-91 3.083998180958993 1103 92-93 3.1455019542203786 1103 94-95 3.4111482650979106 1103 96-97 3.4672850500316024 1103 98-99 3.858830026902119 1103 100-101 3.8114581901349744 1104 1 0.2525707984456105 1104 2 0.24034360776410324 1104 3 0.2687239809544195 1104 4 0.19289326323952594 1104 5 0.08255149914439386 1104 6 0.11395768030648412 1104 7 0.17400978148332058 1104 8 0.2014009254318907 1104 9 0.2634961897268937 1104 10-11 0.29706361338258347 1104 12-13 0.3133106645298085 1104 14-15 0.5134229544045041 1104 16-17 0.4505524486305319 1104 18-19 0.39290656767109766 1104 20-21 0.3864796253933136 1104 22-23 0.45972289487366425 1104 24-25 0.42842072892030814 1104 26-27 0.5377600897443031 1104 28-29 0.602828751537281 1104 30-31 0.5881551774901581 1104 32-33 0.6503591663821382 1104 34-35 0.5847001960881002 1104 36-37 0.644550192817924 1104 38-39 0.7317053366755601 1104 40-41 0.6839051728146543 1104 42-43 0.7509044817640955 1104 44-45 0.763876869946742 1104 46-47 0.849448514462388 1104 48-49 0.8971390430623245 1104 50-51 0.9951779857846503 1104 52-53 1.1069137647204883 1104 54-55 1.1457745596746634 1104 56-57 1.134068423185802 1104 58-59 1.2811981471275686 1104 60-61 1.4093946961868795 1104 62-63 1.5832866082912318 1104 64-65 1.6590342887404645 1104 66-67 1.7608018803770804 1104 68-69 1.8958762383863963 1104 70-71 1.8824816881249724 1104 72-73 2.0644303132465325 1104 74-75 2.035821159296866 1104 76-77 2.088758973064202 1104 78-79 2.2864434244714467 1104 80-81 2.3387251345778104 1104 82-83 2.5652270625500684 1104 84-85 2.576341892560997 1104 86-87 2.708747379223766 1104 88-89 3.0103000999625067 1104 90-91 3.2379163689300334 1104 92-93 3.594208027760228 1104 94-95 3.883583018519122 1104 96-97 4.26129643298945 1104 98-99 4.570343670494118 1104 100-101 4.153800555611195 1105 1 0.19670287887537796 1105 2 0.17799389758832262 1105 3 0.15039461092698758 1105 4 0.10781704919161683 1105 5 -0.006772554164264477 1105 6 0.010792184065159915 1105 7 0.07586473849748643 1105 8 0.059695625217869974 1105 9 0.14845871572700986 1105 10-11 0.11038378961101358 1105 12-13 0.22916690091894054 1105 14-15 0.3301055050489907 1105 16-17 0.3073907659107391 1105 18-19 0.25257956845123175 1105 20-21 0.22244258571115694 1105 22-23 0.3363810164272749 1105 24-25 0.2804322590138142 1105 26-27 0.2660738468865418 1105 28-29 0.27754100322697184 1105 30-31 0.395278295802612 1105 32-33 0.45294860217573074 1105 34-35 0.4363440425644711 1105 36-37 0.5345535894491107 1105 38-39 0.4792510656168716 1105 40-41 0.4791751076054922 1105 42-43 0.5520965660415555 1105 44-45 0.7246969513497135 1105 46-47 0.6513458951950142 1105 48-49 0.8158106561443361 1105 50-51 0.8455005119761196 1105 52-53 0.9119109131221776 1105 54-55 0.9286895902460586 1105 56-57 0.9829837736009353 1105 58-59 1.1096753628741283 1105 60-61 1.1166917978763493 1105 62-63 1.3394586357007512 1105 64-65 1.328739243140518 1105 66-67 1.322081258759404 1105 68-69 1.67149884874188 1105 70-71 1.5850073601983432 1105 72-73 1.859491290293981 1105 74-75 1.6688559091575286 1105 76-77 1.5971914346069198 1105 78-79 1.724836428368004 1105 80-81 2.051117866470502 1105 82-83 2.020104662519177 1105 84-85 2.2981729193858413 1105 86-87 2.4529327840294144 1105 88-89 2.5478720007330367 1105 90-91 2.6752757752235077 1105 92-93 3.004133059257658 1105 94-95 3.0410761611854937 1105 96-97 3.3418652764501964 1105 98-99 3.406568383275463 1105 100-101 3.4154962946843135 1106 1 0.14424971532459807 1106 2 0.11946731395017451 1106 3 0.10935027214148363 1106 4 0.14119341281809028 1106 5 0.008342667399432457 1106 6 0.026109506788571935 1106 7 0.05737561445764783 1106 8 0.18973783073167283 1106 9 0.15681409858260054 1106 10-11 0.18560597313106797 1106 12-13 0.17511242361171497 1106 14-15 0.11753868679546287 1106 16-17 0.13418174731957322 1106 18-19 0.06636906575179324 1106 20-21 -0.027446349316285534 1106 22-23 0.021482353014881994 1106 24-25 0.013124379986358292 1106 26-27 -0.0326772470725345 1106 28-29 0.09394182554461139 1106 30-31 0.22276690535817067 1106 32-33 0.2571417561555407 1106 34-35 0.28902128124281035 1106 36-37 0.25715144984732063 1106 38-39 0.17360611317575803 1106 40-41 0.28209911245921404 1106 42-43 0.342009031182819 1106 44-45 0.42372909120371105 1106 46-47 0.6136011957759777 1106 48-49 0.5502712446873161 1106 50-51 0.7625502669408348 1106 52-53 0.7510842171744727 1106 54-55 0.7502562731610354 1106 56-57 0.8125414473539436 1106 58-59 1.0117486965822167 1106 60-61 0.8621590694398051 1106 62-63 0.8794066663601114 1106 64-65 1.0379347964534347 1106 66-67 1.173667958920575 1106 68-69 1.1460129199114952 1106 70-71 1.0188844269753012 1106 72-73 1.4443378409497427 1106 74-75 1.8192256833960272 1106 76-77 1.9008041659249706 1106 78-79 1.9736345776093245 1106 80-81 1.8274266651736255 1106 82-83 2.0097013157503447 1106 84-85 2.198181227622751 1106 86-87 2.2923448674698292 1106 88-89 2.5086087402346955 1106 90-91 2.6760108234055124 1106 92-93 2.6843880067385584 1106 94-95 2.9808439987146222 1106 96-97 3.3960127268847238 1106 98-99 3.414885933980848 1106 100-101 3.62737636462516 1107 1 0.21241178089395873 1107 2 0.18071288790952877 1107 3 0.1806097136823226 1107 4 0.13546257696904007 1107 5 0.024071568739998384 1107 6 0.08509588089589926 1107 7 0.09038385167130514 1107 8 0.09669529133914523 1107 9 0.03923241051020199 1107 10-11 0.2086861730624392 1107 12-13 0.2516861911776118 1107 14-15 0.35704730500582826 1107 16-17 0.36124946326719254 1107 18-19 0.2677784292053573 1107 20-21 0.2694696815380482 1107 22-23 0.2656771225896435 1107 24-25 0.3172926552780595 1107 26-27 0.28151712162815556 1107 28-29 0.31746072476104104 1107 30-31 0.38764058927279166 1107 32-33 0.4635423344787668 1107 34-35 0.4256949712453917 1107 36-37 0.4699369873753696 1107 38-39 0.48410337500624223 1107 40-41 0.5867052719808115 1107 42-43 0.5497317454229815 1107 44-45 0.5753028213436053 1107 46-47 0.6634918868978517 1107 48-49 0.8313996569615085 1107 50-51 0.7937520757644876 1107 52-53 0.9488902882708956 1107 54-55 1.0822240009685444 1107 56-57 1.0452438559084953 1107 58-59 1.170448670284216 1107 60-61 1.067784097821189 1107 62-63 1.1889770940295463 1107 64-65 1.281012108760784 1107 66-67 1.484248555983413 1107 68-69 1.8079362704602353 1107 70-71 1.831911603409626 1107 72-73 1.818599446711552 1107 74-75 1.6208917393257778 1107 76-77 1.61678513555119 1107 78-79 1.6591204245090303 1107 80-81 1.932157428333067 1107 82-83 2.1067563384612775 1107 84-85 2.1471887149856492 1107 86-87 2.364984330478215 1107 88-89 2.617291107081712 1107 90-91 2.906579949814411 1107 92-93 3.156396517245202 1107 94-95 2.9381142325838887 1107 96-97 3.3658482836830075 1107 98-99 3.7570100808551175 1107 100-101 3.534242755293075 1108 1 0.1313142100201219 1108 2 0.16141692728892565 1108 3 0.13873819062840198 1108 4 0.11389761059743364 1108 5 -0.03034467733426993 1108 6 0.01506461320571617 1108 7 0.07264005055218803 1108 8 0.08496429501783354 1108 9 0.10385201572841396 1108 10-11 0.08515841914499589 1108 12-13 0.08009148717069792 1108 14-15 0.25413194605253153 1108 16-17 0.25690921912845965 1108 18-19 0.21173991050570606 1108 20-21 0.16181598787160567 1108 22-23 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-0.39561742555945756 2316 48-49 -0.18989468501060003 2316 50-51 -0.13153223038367656 2316 52-53 -0.06345630156625504 2316 54-55 0.14292482348467672 2316 56-57 0.23002706234662895 2316 58-59 0.25456723206657017 2316 60-61 0.43418981418992786 2316 62-63 0.4602536723972577 2316 64-65 0.7568051482046982 2316 66-67 0.9562168395782038 2316 68-69 1.21213926176954 2316 70-71 1.2669973356008946 2316 72-73 1.361720290130215 2316 74-75 1.721393516911295 2316 76-77 1.789451658112604 2316 78-79 1.7698162650487603 2316 80-81 1.6358106871309488 2316 82-83 1.9632642561285358 2316 84-85 2.217141993303084 2316 86-87 2.368291831946266 2316 88-89 2.631272288822853 2316 90-91 2.96907646175033 2316 92-93 3.075086170333581 2316 94-95 3.5553646389688787 2316 96-97 3.8784946768818216 2316 98-99 4.281641734481983 2316 100-101 4.6860916946901945 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 8.0 4 20.0 5 52.0 6 108.0 7 204.0 8 576.0 9 1040.0 10 1791.0 11 2949.0 12 4675.0 13 7175.0 14 9665.0 15 13061.0 16 17647.0 17 24327.0 18 26096.0 19 21488.0 20 21242.0 21 22749.0 22 25489.0 23 27827.0 24 31720.0 25 35822.0 26 40462.0 27 47251.0 28 56364.0 29 67905.0 30 81111.0 31 97399.0 32 119398.0 33 148577.0 34 194504.0 35 266853.0 36 370648.0 37 472632.0 38 262855.0 39 12141.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.12718734472659 18.416930528680417 13.929349046525092 23.526533080067896 2 15.895719777393847 19.53846997939168 39.952862566870465 24.61294767634401 3 19.789175729910657 23.39577917323955 29.31466917465641 27.50037592219338 4 13.070967573884348 16.04757456317431 35.735938757379756 35.14551910556159 5 13.96930585331893 37.005088296444036 33.98936981460394 15.036236035633088 6 33.66891148440546 35.19809490432688 16.61713702070694 14.515856590560716 7 29.734627760191362 32.154278274411496 19.66069640628028 18.450397559116865 8 26.90077566249407 35.42082867307014 18.822780024263626 18.855615640172164 9 26.446057895594706 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28.826597764576018 22.746313200099298 34-35 22.73535603353462 26.311785331354827 28.66864635756675 22.284212277543798 36-37 22.668306875299944 26.135872104771284 28.5345982988558 22.66122272107297 38-39 22.33331300703507 26.363465393095808 28.714527402711525 22.588694197157594 40-41 22.57672874143333 26.204000695457363 28.741078104953672 22.47819245815563 42-43 22.496564310347956 26.2311846163197 28.521039208303435 22.751211865028903 44-45 22.396851463976898 26.432255128665073 28.55174289165975 22.61915051569828 46-47 22.53009960782797 26.640328482060998 28.04825251190191 22.781319398209117 48-49 22.628191947627254 26.67379551249745 27.982482693243487 22.715529846631807 50-51 22.590738867575144 26.874913421818373 27.848772434848808 22.685575275757675 52-53 22.885351346594117 26.66744151261506 27.796934056926315 22.650273083864512 54-55 22.46655960900405 26.652622090529103 27.957520550848376 22.923297749618467 56-57 22.552461455188606 26.96371687754912 27.775213594089436 22.708608073172833 58-59 22.665645027679364 26.602342613198875 27.803761646862053 22.928250712259707 60-61 22.568203874203434 26.660712587273768 28.04280622628843 22.728277312234372 62-63 22.53487497419921 26.714761052111875 28.023763959560142 22.72660001412877 64-65 22.26070579914967 26.661422972353787 27.9621380538685 23.115733174628044 66-67 22.317501543607886 26.822034491965052 27.79188791384132 23.068576050585744 68-69 22.455918786574014 26.66912672282041 27.857333845077637 23.017620645527945 70-71 22.3322376301238 26.830889279776088 27.645543103206716 23.191329986893393 72-73 22.478309566996444 26.85037626744588 27.5605642985576 23.110749867000074 74-75 22.358877070624512 27.027488350671337 27.429132181746496 23.184502396957658 76-77 22.364264157481323 26.80269093868311 27.64923315903904 23.18381174479653 78-79 22.4234037153929 26.953095246458236 27.582831886976155 23.040669151172708 80-81 22.352378439721125 27.2050020611046 27.49462228309612 22.947997216078157 82-83 22.410179531417455 27.14733124147399 27.396167915224247 23.04632131188431 84-85 22.38425167638225 26.98839260694707 27.333831939472635 23.293523777198043 86-87 22.46553311317413 26.968364365782644 27.344641241469 23.221461279574232 88-89 22.317911531010086 27.0788136727027 27.241314259757036 23.361960536530173 90-91 22.319891655666932 27.028836634371622 27.427431852136746 23.2238398578247 92-93 22.46218333345502 26.798828574771893 27.359204115971963 23.37978397580112 94-95 22.264527432818745 26.73835771896005 27.33066136298114 23.666453485240062 96-97 22.39672275424406 26.60952666610099 27.287767356595833 23.705983223059118 98-99 22.22860034011659 26.68962131344676 27.027448884833554 24.054329461603093 100-101 22.40888028709029 26.196436471643597 27.29905278042747 24.095630460838642 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1136.0 1 776.0 2 619.5 3 884.0 4 733.0 5 620.5 6 1252.5 7 2601.0 8 4160.5 9 4842.5 10 3980.5 11 2904.5 12 2685.5 13 2801.5 14 2967.0 15 3142.5 16 3296.0 17 3454.5 18 3655.0 19 3818.5 20 4148.0 21 4778.5 22 5628.0 23 6779.5 24 8395.5 25 10505.5 26 13127.5 27 16271.0 28 19109.5 29 22063.0 30 25559.0 31 29431.5 32 34676.5 33 38409.5 34 41203.5 35 45156.5 36 49895.0 37 55371.0 38 59631.0 39 62063.0 40 65687.0 41 71383.5 42 76156.5 43 81494.5 44 86980.0 45 92430.0 46 94852.5 47 96447.5 48 101277.0 49 102929.0 50 102007.0 51 99487.5 52 95513.0 53 93811.0 54 92547.0 55 87027.5 56 78999.5 57 72119.5 58 65393.5 59 57096.5 60 47715.5 61 38706.5 62 30922.0 63 23924.0 64 18515.0 65 14652.5 66 11153.0 67 8718.5 68 6471.0 69 4429.5 70 3259.5 71 2413.0 72 1865.5 73 1517.0 74 1255.0 75 1062.5 76 724.0 77 397.0 78 216.0 79 125.5 80 73.0 81 48.5 82 34.0 83 16.0 84 7.0 85 4.5 86 2.0 87 1.0 88 1.0 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.015549540084859443 3 0.0026836769689605132 4 0.0018154285378262293 5 0.11567437052975389 6 3.946583777883107E-5 7 0.0 8 1.1839751333649324E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 1.9732918889415536E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.7759627000473984E-4 24-25 6.117204855718817E-4 26-27 0.005840943991266999 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010912304145846792 36-37 5.13055891124804E-4 38-39 0.006847322854627192 40-41 0.009965124039154848 42-43 0.00548575145125752 44-45 0.0034729937245371345 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 1.9732918889415536E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.9732918889415536E-5 68-69 7.695838366872061E-4 70-71 0.0 72-73 3.946583777883107E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 5.919875666824662E-5 82-83 0.001835161456715645 84-85 0.004222844642334926 86-87 0.0025455465367346043 88-89 0.0 90-91 0.0025060806989557734 92-93 1.9732918889415536E-5 94-95 5.919875666824662E-5 96-97 7.893167555766215E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2533837.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.33972196238577 #Duplication Level Percentage of deduplicated Percentage of total 1 74.17812967531347 38.082845532203905 2 13.920224845347684 14.29320946428089 3 4.801089804611071 7.39459847055532 4 2.235960229746929 4.591743060566382 5 1.2532086107641724 3.2169690818750167 6 0.7800834772493789 2.4029561297660518 7 0.515520315939086 1.8526668780391757 8 0.36931285728034025 1.5168335527925538 9 0.28164807257786745 1.3013760363650606 >10 1.508354914649686 14.024112048168714 >50 0.09999217044568513 3.5349899985136006 >100 0.05132128914553476 4.699404477388612 >500 0.0037691508884416455 1.2622590432299499 >1k 0.0011538217005403004 0.8088599231417852 >5k 1.538428934053734E-4 0.5844706016257407 >10k+ 7.69214467026867E-5 0.4327057014873812 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10957 0.43242718454265217 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8997 0.3550741424961432 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5803 0.22902025663055675 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.946583777883108E-5 0.0 3 0.0 0.0 0.0 7.893167555766216E-5 0.0 4 0.0 0.0 0.0 7.893167555766216E-5 0.0 5 0.0 0.0 0.0 7.893167555766216E-5 0.0 6 0.0 0.0 0.0 7.893167555766216E-5 0.0 7 0.0 0.0 0.0 7.893167555766216E-5 0.0 8 0.0 0.0 0.0 7.893167555766216E-5 0.0 9 0.0 0.0 0.0 7.893167555766216E-5 0.0 10-11 0.0 0.0 0.0 7.893167555766216E-5 0.0 12-13 0.0 0.0 0.0 7.893167555766216E-5 0.0 14-15 0.0 0.0 0.0 7.893167555766216E-5 0.0 16-17 0.0 0.0 0.0 7.893167555766216E-5 0.0 18-19 0.0 0.0 0.0 7.893167555766216E-5 0.0 20-21 0.0 0.0 0.0 2.1706210778357093E-4 0.0 22-23 0.0 0.0 0.0 4.538571344565574E-4 0.0 24-25 0.0 0.0 0.0 6.511863233507127E-4 0.0 26-27 0.0 0.0 0.0 0.001361571403369672 0.0 28-29 0.0 0.0 0.0 0.0035519254000947968 0.0 30-31 1.973291888941554E-5 0.0 0.0 0.011405627118082181 0.0 32-33 3.946583777883108E-5 0.0 0.0 0.029086322442998504 0.0 34-35 3.946583777883108E-5 0.0 0.0 0.05805424737266052 0.0 36-37 3.946583777883108E-5 0.0 0.0 0.10797853216288182 0.0 38-39 3.946583777883108E-5 0.0 0.0 0.18049700908148394 0.0 40-41 3.946583777883108E-5 0.0 0.0 0.2581657778302235 0.0 42-43 3.946583777883108E-5 0.0 0.0 0.33423618014892037 0.0 44-45 5.919875666824662E-5 0.0 0.0 0.42745448898251936 0.0 46-47 7.893167555766216E-5 0.0 0.0 0.5335781267697961 0.0 48-49 7.893167555766216E-5 0.0 0.0 0.6468450811950414 0.0 50-51 7.893167555766216E-5 0.0 0.0 0.7654004578826499 0.0 52-53 7.893167555766216E-5 0.0 0.0 0.878667412307895 0.0 54-55 7.893167555766216E-5 0.0 0.0 1.0008536460711561 0.0 56-57 7.893167555766216E-5 0.0 0.0 1.142930662074948 0.0 58-59 7.893167555766216E-5 0.0 0.0 1.2974788828168504 0.0 60-61 7.893167555766216E-5 0.0 0.0 1.4635708611090612 0.0 62-63 7.893167555766216E-5 0.0 0.0 1.6231115103299856 0.0 64-65 7.893167555766216E-5 0.0 0.0 1.785533165708765 0.0 66-67 7.893167555766216E-5 0.0 0.0 1.9716343237548428 0.0 68-69 7.893167555766216E-5 0.0 0.0 2.1768566802047644 0.0 70-71 7.893167555766216E-5 0.0 0.0 2.398970415224026 0.0 72-73 7.893167555766216E-5 0.0 0.0 2.6189727279221198 3.946583777883108E-5 74-75 7.893167555766216E-5 0.0 0.0 2.836271630732363 3.946583777883108E-5 76-77 7.893167555766216E-5 0.0 0.0 3.069218738221914 3.946583777883108E-5 78-79 7.893167555766216E-5 0.0 0.0 3.326200540918773 3.946583777883108E-5 80-81 7.893167555766216E-5 0.0 0.0 3.59875161661938 3.946583777883108E-5 82-83 7.893167555766216E-5 0.0 0.0 3.8761964562045623 3.946583777883108E-5 84-85 7.893167555766216E-5 0.0 0.0 4.151589072225246 3.946583777883108E-5 86-87 7.893167555766216E-5 0.0 0.0 4.436611352663964 7.893167555766216E-5 88-89 7.893167555766216E-5 0.0 0.0 4.746201117120004 7.893167555766216E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4445 0.0 59.703423 1 GTATCAA 8435 0.0 41.329075 1 ATCAACG 10540 0.0 32.707577 3 TCAACGC 10750 0.0 32.02314 4 TATCAAC 10850 0.0 31.910856 2 CAACGCA 10880 0.0 31.684214 5 AACGCAG 11210 0.0 30.802252 6 GTGGTAT 1885 0.0 30.024267 1 TGGTATC 1910 0.0 28.884272 2 ACGCAGA 12920 0.0 26.615198 7 GTACATG 9730 0.0 26.492517 1 CGCAGAG 13045 0.0 26.287346 8 TACATGG 9895 0.0 25.281727 2 ACATGGG 9970 0.0 24.275803 3 GCAGAGT 14380 0.0 23.483583 9 GGACCGA 595 0.0 23.149128 6 GTGTAGC 1400 0.0 22.760593 1 GAGTACT 9070 0.0 21.54846 12-13 CAGAGTA 14100 0.0 21.170923 10-11 AGAGTAC 13540 0.0 20.731106 10-11 >>END_MODULE