FastQCFastQC Report
Thu 26 May 2016
SRR938014_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938014_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2537892
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108810.42874164858079067No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90090.3549796445238805No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54740.21569081741855053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48600.055.0000421
GTATCAA86100.042.1883741
ATCAACG107650.033.117343
TATCAAC110350.032.565492
TCAACGC110250.032.422574
CAACGCA110600.032.234015
AACGCAG113300.031.50476
GTACATG98300.027.435081
GTGGTAT21000.027.2755321
ACGCAGA130850.027.0137397
CGCAGAG131950.026.6445148
TACATGG100650.026.0695462
ACATGGG101450.024.926873
TGGTATC22900.024.2861442
GCAGAGT145000.023.8837329
GAGTACT85450.023.09922212-13
CATGGGG52800.022.0566924
GTACTTT92050.021.4440614-15
CAGAGTA141150.020.95061710-11
GTGTAAG9850.020.3528061
Produced by FastQC (version 0.11.5)