FastQCFastQC Report
Thu 26 May 2016
SRR938014_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938014_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2537892
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109370.43094820425770675No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93130.3669580896271394No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58250.22952119317922118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47200.056.4636761
GTATCAA85950.041.5090071
ATCAACG106950.032.821023
TCAACGC108500.032.3933684
TATCAAC109550.032.2633062
CAACGCA111150.031.663795
AACGCAG114300.030.8742736
GTGGTAT21600.028.8114951
GTACATG95250.027.5809021
TGGTATC21900.026.8983652
ACGCAGA132450.026.5359067
TACATGG97500.026.3110052
CGCAGAG135250.026.0216668
ACATGGG97350.025.2744773
GCAGAGT148550.023.3721629
GAGTACT93700.022.20226112-13
GGACCGA4600.021.6828146
GTACTTT97800.021.10151514-15
CAGAGTA145150.020.76218410-11
TAAGGTG11800.020.5277985
Produced by FastQC (version 0.11.5)