FastQCFastQC Report
Thu 26 May 2016
SRR938013_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938013_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2538618
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113270.44618765013089795No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91330.3597626740218497No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55780.21972585083695145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46000.056.6584241
GTATCAA83200.040.906831
ATCAACG105550.031.5183563
TATCAAC106200.031.3254472
TCAACGC106700.031.1341154
CAACGCA108400.030.7335345
AACGCAG111250.029.9034846
GTGGTAT20450.027.543451
GTACATG95700.027.2339321
ACGCAGA129050.025.6401567
TACATGG99100.025.4170992
TGGTATC21150.025.3917032
CGCAGAG130300.025.1382858
ACATGGG99550.024.2519253
GAGTACT87200.022.43217312-13
GCAGAGT143500.022.4279219
GTACTTT91600.020.97834814-15
CATGGGG52750.020.5416894
CAGAGTA140350.020.47497210-11
AGTACTT90750.020.11419312-13
Produced by FastQC (version 0.11.5)