##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938013_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2538618 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.88437803560835 33.0 31.0 34.0 30.0 34.0 2 32.06730315470858 34.0 31.0 34.0 30.0 34.0 3 32.13949755339323 34.0 31.0 34.0 30.0 34.0 4 35.65426385537328 37.0 35.0 37.0 33.0 37.0 5 35.67636012980291 37.0 35.0 37.0 35.0 37.0 6 35.70364544803511 37.0 35.0 37.0 35.0 37.0 7 35.64132531952424 37.0 35.0 37.0 33.0 37.0 8 35.622604503710285 37.0 35.0 37.0 33.0 37.0 9 37.28202037486538 39.0 37.0 39.0 34.0 39.0 10-11 37.20121203741563 39.0 37.0 39.0 34.0 39.0 12-13 37.10050369925684 39.0 37.0 39.0 33.5 39.0 14-15 38.358446800582044 40.0 38.0 41.0 33.5 41.0 16-17 38.27872015403656 40.0 38.0 41.0 33.0 41.0 18-19 38.20321253532434 40.0 38.0 41.0 33.0 41.0 20-21 38.097079986039645 40.0 38.0 41.0 33.0 41.0 22-23 37.936090227044794 40.0 38.0 41.0 33.0 41.0 24-25 37.890821896007985 40.0 38.0 41.0 33.0 41.0 26-27 37.916254828414516 40.0 38.0 41.0 33.0 41.0 28-29 37.82503098142375 40.0 38.0 41.0 33.0 41.0 30-31 37.65187869935532 40.0 37.5 41.0 32.5 41.0 32-33 37.451881102237515 40.0 37.0 41.0 32.0 41.0 34-35 37.25076281661912 40.0 37.0 41.0 31.0 41.0 36-37 37.039718460989405 40.0 36.5 41.0 31.0 41.0 38-39 36.81362359362456 40.0 36.0 41.0 30.0 41.0 40-41 36.59868578100368 39.0 36.0 41.0 30.0 41.0 42-43 36.32471545541708 39.0 35.0 41.0 30.0 41.0 44-45 36.10488955014106 39.0 35.0 41.0 29.0 41.0 46-47 35.974341945105564 39.0 35.0 41.0 28.5 41.0 48-49 35.74347203872343 39.0 35.0 41.0 27.5 41.0 50-51 35.10683253644306 38.5 34.5 40.5 26.5 40.5 52-53 35.192888020174756 38.5 35.0 40.0 27.0 41.0 54-55 35.32913459212847 39.0 35.0 40.5 26.5 41.0 56-57 35.11963714115318 39.0 35.0 40.0 26.0 41.0 58-59 34.85819095271522 38.0 34.5 40.0 26.0 41.0 60-61 34.48498119843158 38.0 34.0 40.0 25.0 41.0 62-63 34.06480868724637 37.0 34.0 40.0 23.5 41.0 64-65 33.63056080119183 37.0 33.5 40.0 22.5 41.0 66-67 33.20065011750488 36.0 33.0 39.0 21.5 41.0 68-69 32.70519767054358 35.5 32.5 39.0 20.0 40.5 70-71 32.21457816812139 35.0 32.0 38.5 18.0 40.0 72-73 31.71584421129922 35.0 31.0 37.5 15.0 40.0 74-75 31.183082488188454 35.0 31.0 37.0 10.0 39.0 76-77 30.633506301460088 35.0 30.5 36.5 7.0 39.0 78-79 30.0573644794136 34.0 30.0 36.0 3.5 38.5 80-81 29.51276481928356 34.0 29.0 35.0 2.0 37.0 82-83 28.965994292957824 34.0 29.0 35.0 2.0 37.0 84-85 28.464479295427672 34.0 28.5 35.0 2.0 36.0 86-87 27.94484262697263 34.0 27.0 35.0 2.0 36.0 88-89 27.380157629072194 33.0 26.0 35.0 2.0 35.5 90-91 26.753439272864213 33.0 25.0 35.0 2.0 35.0 92-93 26.116423581649542 32.0 24.0 35.0 2.0 35.0 94-95 25.482509381088448 32.0 21.5 34.5 2.0 35.0 96-97 24.613812121398336 32.0 12.5 34.0 2.0 35.0 98-99 23.507892286275446 31.0 2.0 34.0 2.0 35.0 100-101 20.561102536892122 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3853518739156563 1101 2 0.20371090373375722 1101 3 0.20573374440164827 1101 4 0.182483744495201 1101 5 0.1882891277435732 1101 6 0.15791142723752216 1101 7 0.27436494379527687 1101 8 0.2330164814470237 1101 9 0.17234952166247552 1101 10-11 0.26841134550154777 1101 12-13 0.15394486801795892 1101 14-15 0.29886713131164555 1101 16-17 0.41129318431954687 1101 18-19 0.32881790487142837 1101 20-21 0.5081780249877923 1101 22-23 0.4038510242131892 1101 24-25 0.43008040405648273 1101 26-27 0.43879564986851705 1101 28-29 0.4314577692048047 1101 30-31 0.3970332008970061 1101 32-33 0.45677061748256165 1101 34-35 0.5160753367829116 1101 36-37 0.47982823601770974 1101 38-39 0.4731058074923027 1101 40-41 0.5389606669902136 1101 42-43 0.4822334352873128 1101 44-45 0.56593525893134 1101 46-47 0.6440763063927335 1101 48-49 0.729488186588263 1101 50-51 0.7228342658378253 1101 52-53 0.8376286630470915 1101 54-55 0.7229688252389153 1101 56-57 0.7059906993797256 1101 58-59 0.6612216408770308 1101 60-61 0.5812940052447146 1101 62-63 0.6815214427126364 1101 64-65 0.44671680396814395 1101 66-67 0.37139660107624906 1101 68-69 0.5069694099832347 1101 70-71 0.6590581496403729 1101 72-73 0.7937555357500905 1101 74-75 0.8253426342395294 1101 76-77 0.8283334413361381 1101 78-79 0.7284982816102428 1101 80-81 0.8711547636286845 1101 82-83 0.7185821324619255 1101 84-85 0.7117188779825199 1101 86-87 0.6923529113971192 1101 88-89 0.7612461027802837 1101 90-91 0.7085079353871144 1101 92-93 0.7867291787689901 1101 94-95 1.00963903721944 1101 96-97 0.8637556870048222 1101 98-99 0.9376754279820432 1101 100-101 1.0783985688616262 1102 1 0.45185929045980444 1102 2 0.37503788125255255 1102 3 0.31369622606121794 1102 4 0.24865169331007309 1102 5 0.3457072866614439 1102 6 0.3426165527231433 1102 7 0.2701856373076694 1102 8 0.42579873693667025 1102 9 0.49113412443384874 1102 10-11 0.47557189428691515 1102 12-13 0.40640233913384094 1102 14-15 0.4193572566541448 1102 16-17 0.4411245991403021 1102 18-19 0.3935733926883884 1102 20-21 0.50522337127056 1102 22-23 0.5137077358021429 1102 24-25 0.3482079441008281 1102 26-27 0.43172196812263763 1102 28-29 0.4921612050586148 1102 30-31 0.4537833056732623 1102 32-33 0.4523758866089125 1102 34-35 0.4009883449661018 1102 36-37 0.566812070747936 1102 38-39 0.5294758919078362 1102 40-41 0.6847470100383148 1102 42-43 0.5545818987279176 1102 44-45 0.5014410695178952 1102 46-47 0.572238237270497 1102 48-49 0.5213002433076284 1102 50-51 0.6467064255938979 1102 52-53 0.640657284030091 1102 54-55 0.5039473657405367 1102 56-57 0.5454540247542212 1102 58-59 0.5200003130554336 1102 60-61 0.6704565805606748 1102 62-63 0.6353849341143771 1102 64-65 0.4460651176759569 1102 66-67 0.46418909564660993 1102 68-69 0.4385627173677804 1102 70-71 0.4569891580540286 1102 72-73 0.5597710727293226 1102 74-75 0.5240247471160409 1102 76-77 0.5404338878187751 1102 78-79 0.5492992309455111 1102 80-81 0.5617899316046575 1102 82-83 0.6613923711022345 1102 84-85 0.5587375622863391 1102 86-87 0.39622420138183045 1102 88-89 0.685621684004083 1102 90-91 0.8248884827485519 1102 92-93 0.8089402770516863 1102 94-95 1.0270285927699376 1102 96-97 0.9867349365352567 1102 98-99 0.9350803348137475 1102 100-101 0.9659935556021999 1103 1 0.4066804997874236 1103 2 0.3369955651235159 1103 3 0.29928308231637146 1103 4 0.1973354920471877 1103 5 0.18720412661720331 1103 6 0.1847648022016628 1103 7 0.28304531095149343 1103 8 0.2739555312087987 1103 9 0.28443090802852566 1103 10-11 0.2859924286976252 1103 12-13 0.1870867368844955 1103 14-15 0.27665290169826307 1103 16-17 0.3143788924246991 1103 18-19 0.3894672115200777 1103 20-21 0.31062114883120273 1103 22-23 0.36537010458206254 1103 24-25 0.48137800022117005 1103 26-27 0.31459588072896594 1103 28-29 0.4717689007540713 1103 30-31 0.4942852237910884 1103 32-33 0.5587517697290494 1103 34-35 0.5651954096439908 1103 36-37 0.44578885507898747 1103 38-39 0.5227169870688542 1103 40-41 0.6018441734330722 1103 42-43 0.5918945653860916 1103 44-45 0.5549095774077344 1103 46-47 0.7386324802264284 1103 48-49 0.6617924610879982 1103 50-51 0.5881232908696674 1103 52-53 0.6493024442697575 1103 54-55 0.6190418696850983 1103 56-57 0.5548190987246073 1103 58-59 0.7466646289833818 1103 60-61 0.7310210269121455 1103 62-63 0.6711319167429863 1103 64-65 0.8563322168218974 1103 66-67 0.9375818488572776 1103 68-69 1.0856214263590687 1103 70-71 1.115864754412371 1103 72-73 1.1631465777487158 1103 74-75 0.9495918274277564 1103 76-77 0.9487039211397885 1103 78-79 0.9151143566746285 1103 80-81 0.8390046885793403 1103 82-83 0.9098950142671782 1103 84-85 0.9789903445838597 1103 86-87 0.7905892965938293 1103 88-89 0.9081916786653537 1103 90-91 0.9883289021429924 1103 92-93 1.0484189511784088 1103 94-95 1.0409128203044524 1103 96-97 1.2289183762890836 1103 98-99 1.3429673110132292 1103 100-101 1.0968279191538564 1104 1 0.3496081442978145 1104 2 0.2878779253775505 1104 3 0.2761231836260194 1104 4 0.22400352957933478 1104 5 0.25195164881144905 1104 6 0.19748159550538702 1104 7 0.18842837713405913 1104 8 0.20500469353607542 1104 9 0.28641664699558333 1104 10-11 0.29482207917396863 1104 12-13 0.2127689608583765 1104 14-15 0.2641034346222213 1104 16-17 0.3992008579904436 1104 18-19 0.39864814627561174 1104 20-21 0.46109665099468344 1104 22-23 0.40298070621057747 1104 24-25 0.5059331241333069 1104 26-27 0.5364980103416102 1104 28-29 0.553801927860242 1104 30-31 0.3837264348297822 1104 32-33 0.4672233151984386 1104 34-35 0.5157637812237965 1104 36-37 0.5067814490454339 1104 38-39 0.5478253291212951 1104 40-41 0.6059517299258346 1104 42-43 0.6255723286712254 1104 44-45 0.559892404629629 1104 46-47 0.6198256506283712 1104 48-49 0.5958274557893333 1104 50-51 0.6402124831409495 1104 52-53 0.5938521115996807 1104 54-55 0.6411228838029999 1104 56-57 0.6225250695829629 1104 58-59 0.588196779474842 1104 60-61 0.4700724148798443 1104 62-63 0.5510869402593741 1104 64-65 0.6482780125320318 1104 66-67 0.5974010760520159 1104 68-69 0.7554844271701668 1104 70-71 0.7355488707182118 1104 72-73 0.891071107572099 1104 74-75 0.7104268726629535 1104 76-77 0.6712600678250844 1104 78-79 0.7879958151142219 1104 80-81 0.8685130720265484 1104 82-83 0.8397372773779246 1104 84-85 0.8585253725488435 1104 86-87 0.9240595148772783 1104 88-89 1.027330136750809 1104 90-91 1.0769997069418302 1104 92-93 1.0618003454426805 1104 94-95 1.2028647849092415 1104 96-97 1.202456808570009 1104 98-99 1.0915307582291938 1104 100-101 1.0369328575623484 1105 1 0.29254828850310943 1105 2 0.3540333867499186 1105 3 0.3018049767246538 1105 4 0.29504386599757026 1105 5 0.21843245408713585 1105 6 0.27932129312138443 1105 7 0.15155276654432726 1105 8 0.2496721129555226 1105 9 0.37006134768201804 1105 10-11 0.3488562240905111 1105 12-13 0.2624850749560821 1105 14-15 0.36888173257711543 1105 16-17 0.4077859814698783 1105 18-19 0.47449506390389473 1105 20-21 0.4720718588899757 1105 22-23 0.3999671010058279 1105 24-25 0.4022610803466051 1105 26-27 0.38741412682900034 1105 28-29 0.46737716713595745 1105 30-31 0.475325427637749 1105 32-33 0.47882275135704333 1105 34-35 0.5450442608725865 1105 36-37 0.5877414415241873 1105 38-39 0.5719395161215601 1105 40-41 0.505382197296008 1105 42-43 0.6495346444959793 1105 44-45 0.7123102220427455 1105 46-47 0.6198034830965327 1105 48-49 0.5615563729730084 1105 50-51 0.5339462092640801 1105 52-53 0.4647560312280703 1105 54-55 0.568819312503571 1105 56-57 0.6546844198636137 1105 58-59 0.5853480158099842 1105 60-61 0.5165826479470681 1105 62-63 0.587695282734849 1105 64-65 0.6604751073590762 1105 66-67 0.7310663406616058 1105 68-69 0.6990516295364628 1105 70-71 0.6540666049008905 1105 72-73 0.8401724106770949 1105 74-75 0.7862225376999099 1105 76-77 0.8313436850128788 1105 78-79 0.8700869019741582 1105 80-81 0.9104584443105885 1105 82-83 0.8375891492137484 1105 84-85 0.928257588764346 1105 86-87 0.9871646609833213 1105 88-89 0.9956262427801548 1105 90-91 0.9803056405769546 1105 92-93 1.0899821959253515 1105 94-95 1.3529075320858688 1105 96-97 1.2068030597573838 1105 98-99 1.3149631285483458 1105 100-101 1.1717943828080237 1106 1 0.0880843240693494 1106 2 0.08607651693294116 1106 3 0.07183572159924978 1106 4 0.08087403555345674 1106 5 0.04611808440163401 1106 6 0.06645381877405754 1106 7 0.19952035710235094 1106 8 0.10283317158710048 1106 9 0.18402588544365983 1106 10-11 -0.4187381372988952 1106 12-13 -0.16138302962106366 1106 14-15 -0.07698506221547774 1106 16-17 0.15326636307833752 1106 18-19 0.22626050966000122 1106 20-21 0.057332801073144424 1106 22-23 0.12687590844520713 1106 24-25 0.18538907642676605 1106 26-27 0.2028525436298949 1106 28-29 0.16191932047761526 1106 30-31 0.22714844389209787 1106 32-33 0.18633245514308783 1106 34-35 0.10967089650615236 1106 36-37 -0.023182274519982116 1106 38-39 0.19939554786066083 1106 40-41 0.42565649137770123 1106 42-43 0.3002686200635054 1106 44-45 0.3024685193703718 1106 46-47 0.40766161988047145 1106 48-49 0.38434754523908765 1106 50-51 0.4742743507041709 1106 52-53 0.3583021629511549 1106 54-55 0.04921548073006221 1106 56-57 0.0473424059391192 1106 58-59 0.16085324707400872 1106 60-61 0.014216463327471729 1106 62-63 0.19694782007098155 1106 64-65 0.19181544661347516 1106 66-67 0.07236854521797653 1106 68-69 -0.08634917552938504 1106 70-71 0.1697388544944829 1106 72-73 0.3119561809370772 1106 74-75 0.29901017105175853 1106 76-77 0.42502187974467986 1106 78-79 0.4176300308200531 1106 80-81 0.21405969811432968 1106 82-83 0.23535174422735494 1106 84-85 0.09819656255053033 1106 86-87 0.24280656981007454 1106 88-89 0.14351215008674956 1106 90-91 -0.04975150798523842 1106 92-93 0.04080413921417758 1106 94-95 0.07844631023635529 1106 96-97 0.28556930689483195 1106 98-99 0.5159695202850578 1106 100-101 0.2431439155138584 1107 1 0.302980791038312 1107 2 0.20473376584858727 1107 3 0.21205113719167912 1107 4 0.14165323342587044 1107 5 0.13262792344696805 1107 6 0.1947903380621625 1107 7 0.15521458790902898 1107 8 0.2299971488039958 1107 9 0.31868840521509867 1107 10-11 0.22130018005884722 1107 12-13 0.05802393038251097 1107 14-15 0.17631108382914107 1107 16-17 0.2540152151610826 1107 18-19 0.34805026413341267 1107 20-21 0.29859084852001416 1107 22-23 0.32801970247638934 1107 24-25 0.35616488981877126 1107 26-27 0.3063295961655115 1107 28-29 0.34150952919296884 1107 30-31 0.40003154015908393 1107 32-33 0.35633287319227946 1107 34-35 0.3935974492725549 1107 36-37 0.2790064420460965 1107 38-39 0.35217133720051663 1107 40-41 0.3291044522712028 1107 42-43 0.381087406621333 1107 44-45 0.32553424013871535 1107 46-47 0.42937812984551016 1107 48-49 0.16602164325501434 1107 50-51 0.16497965695096894 1107 52-53 0.20786558788498155 1107 54-55 0.3844568554590424 1107 56-57 0.3872789598164985 1107 58-59 0.2839807589483314 1107 60-61 0.2776644757612914 1107 62-63 0.352713663276198 1107 64-65 0.36350194866420793 1107 66-67 0.3568863798835338 1107 68-69 0.34874625421944927 1107 70-71 0.406282824678982 1107 72-73 0.5307933341710047 1107 74-75 0.5174888327904092 1107 76-77 0.6591348652687223 1107 78-79 0.5252943041073941 1107 80-81 0.6324497677853778 1107 82-83 0.7252149104288215 1107 84-85 0.6289762930061613 1107 86-87 0.6015282784744613 1107 88-89 0.6148408478463523 1107 90-91 0.6327714875834438 1107 92-93 0.6406650662754707 1107 94-95 0.6455258816042253 1107 96-97 0.6965904006260395 1107 98-99 0.7157396218652821 1107 100-101 0.6624793203859092 1108 1 0.22839308489138332 1108 2 0.17587319613344476 1108 3 0.12959236657698625 1108 4 0.20199806101207685 1108 5 0.18772537472133166 1108 6 0.1899177743440177 1108 7 0.13722358341127716 1108 8 0.1640301323122415 1108 9 0.28233158362588995 1108 10-11 0.24547559235270455 1108 12-13 0.0756401222865577 1108 14-15 0.17068889492359318 1108 16-17 0.3522161147113039 1108 18-19 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-0.8737113952225997 2316 66-67 -0.9667012125376644 2316 68-69 -1.1864704542228068 2316 70-71 -1.2939707989325093 2316 72-73 -1.2197080871467847 2316 74-75 -1.1768831016284302 2316 76-77 -1.123416967517901 2316 78-79 -1.0917964901500632 2316 80-81 -0.9180691439406097 2316 82-83 -1.1804464224672095 2316 84-85 -1.258116966154983 2316 86-87 -1.3056472820690068 2316 88-89 -1.3877837287918453 2316 90-91 -1.2844084468313355 2316 92-93 -1.4147752756937386 2316 94-95 -1.5618389637414722 2316 96-97 -1.6779428288648681 2316 98-99 -1.749892170264392 2316 100-101 -1.203597715596608 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6443.0 3 1912.0 4 1981.0 5 2279.0 6 2489.0 7 3107.0 8 3839.0 9 4884.0 10 6038.0 11 7359.0 12 9051.0 13 11149.0 14 12925.0 15 15163.0 16 18674.0 17 24204.0 18 22327.0 19 18202.0 20 18251.0 21 19079.0 22 20598.0 23 22839.0 24 25477.0 25 28511.0 26 32604.0 27 37712.0 28 44472.0 29 51769.0 30 62682.0 31 77175.0 32 95652.0 33 123379.0 34 169370.0 35 245854.0 36 367768.0 37 507809.0 38 375079.0 39 40505.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.86384418977495 18.01848662985175 13.574332482235812 23.54333669813748 2 15.701593734937669 19.262678368533127 40.42795442372879 24.607773472800414 3 19.695646347519688 23.453046295493422 29.10049740262812 27.750809954358775 4 12.867263467131892 15.825868071910895 35.999870712065125 35.30699774889208 5 14.035549734896763 37.03115125648566 33.81652072748948 15.116778281128095 6 33.46412615736861 35.05134637081461 16.863046505327475 14.621480966489301 7 29.738211185980745 32.16941140483449 19.774925951592014 18.31745145759275 8 26.89644789327797 35.23532849902829 18.97632231012465 18.891901297569092 9 26.70066029831264 16.548248597545932 19.69599635523529 37.055094748906136 10-11 25.63638911670982 26.286915614666135 26.54792251946366 21.528772749160378 12-13 26.27791282929858 23.445682309004408 27.434836299351282 22.841568562345728 14-15 22.955438795494416 24.95809368332974 25.495771531451712 26.590695989724132 16-17 22.767927703083803 28.421543869302386 26.158934654740683 22.651593772873127 18-19 22.70980041830705 26.647889555586694 28.598832973379583 22.043477052726672 20-21 22.996670274378616 25.878786003286557 28.946477809417804 22.17806591291702 22-23 22.90588938309134 25.74041022131896 28.85016074280678 22.503539652782926 24-25 22.681359642378666 26.011132690983846 28.88936464491384 22.41814302172365 26-27 22.35722315774868 26.44270793915084 28.69388417972199 22.506184723378492 28-29 22.327365940609358 26.58581464449891 28.811741741291925 22.275077673599807 30-31 22.361102707019707 26.184058733988397 28.807863154805467 22.646975404186424 32-33 22.14042606534074 26.356830708366548 28.742775329806047 22.759967896486668 34-35 22.783946362516993 26.4190542680183 28.550062511493618 22.246936857971086 36-37 22.69205738277137 26.171399356448273 28.458479560748152 22.678063700032205 38-39 22.354499856556924 26.394373090709617 28.63088313645377 22.620243916279687 40-41 22.600558617937843 26.29009709311777 28.614322096935485 22.495022192008907 42-43 22.55939857209344 26.234265895479954 28.482389191826325 22.723946340600286 44-45 22.390799770061232 26.48974593657299 28.504566664315796 22.61488762904998 46-47 22.524391315466268 26.69639870270236 28.012077744867685 22.767132236963683 48-49 22.667987966452646 26.692409684365646 27.89052387697727 22.749078472204438 50-51 22.609231174160136 26.927472498029413 27.7684647222793 22.694831605531146 52-53 22.898658536297063 26.707818913826543 27.758361590780677 22.63516095909572 54-55 22.49620517003104 26.68764781613815 27.93367198681635 22.88247502701446 56-57 22.517647397212077 27.034230562449157 27.80336570749854 22.644756332840224 58-59 22.71304573947921 26.552163651908735 27.83487059064565 22.89992001796641 60-61 22.576634370426234 26.609596118698388 28.143830074452953 22.66993943642243 62-63 22.5311470449411 26.74717763763917 28.074694597028827 22.646980720390903 64-65 22.325203428212323 26.653868164925342 28.06774472209554 22.953183684766795 66-67 22.321550282047827 26.85880116081257 27.885760189613045 22.93388836752656 68-69 22.490249316875975 26.65857154930027 27.980417275458496 22.870761858365256 70-71 22.365966619607164 26.820291479931075 27.79011789834162 23.02362400212014 72-73 22.549359005303117 26.796957430228467 27.747814667661018 22.90586889680739 74-75 22.42501951977896 27.02301846414458 27.671215009946565 22.88074700612989 76-77 22.56949677301408 26.827786455701514 27.603125943761142 22.99959082752326 78-79 22.664662667203782 26.98729344835975 27.515293464118596 22.832750420317875 80-81 22.58878528974447 27.20790149877748 27.437611348505754 22.765701862972293 82-83 22.688825656539073 27.184700888308573 27.370254388944094 22.756219066208264 84-85 22.660495806814843 27.033574636174308 27.342872767187014 22.96305678982383 86-87 22.761712269013103 27.00686994767715 27.339878335525352 22.8915394477844 88-89 22.727092704624265 27.01213508491131 27.261370620078075 22.999401590386352 90-91 22.707388212777975 27.13556272907298 27.30655396786579 22.850495090283257 92-93 22.90381815351818 26.836719213317334 27.34508392293158 22.914378710232906 94-95 22.79079538145266 26.697725251926496 27.378876641148203 23.132602725472637 96-97 23.004528874134188 26.597362123920586 27.239156239064023 23.158952762881203 98-99 22.934459210116973 26.586465875061645 27.034164638792035 23.444910276029347 100-101 23.243445652982377 26.11525060178639 27.317915843224018 23.323387902007216 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1217.0 1 836.5 2 719.5 3 951.5 4 723.5 5 660.0 6 1339.0 7 2729.0 8 4353.0 9 5035.5 10 4077.0 11 3027.5 12 2862.0 13 2893.5 14 3004.5 15 3133.5 16 3208.0 17 3352.0 18 3562.5 19 3884.5 20 4205.5 21 4780.0 22 5765.5 23 6861.0 24 8375.0 25 10640.5 26 13181.0 27 16175.0 28 19195.0 29 22063.5 30 25456.5 31 29493.5 32 34712.0 33 38420.5 34 41465.0 35 45530.5 36 50044.5 37 55461.0 38 59639.0 39 62341.5 40 66114.0 41 71515.5 42 76081.0 43 81308.5 44 87572.0 45 93036.0 46 94952.0 47 96817.0 48 101431.0 49 103153.0 50 102202.5 51 99321.5 52 95886.0 53 93981.5 54 92237.5 55 86711.0 56 78443.0 57 71538.5 58 64971.5 59 56797.0 60 47402.5 61 38408.0 62 30942.5 63 24182.0 64 18666.0 65 14698.5 66 11346.0 67 8986.0 68 6672.0 69 4612.5 70 3447.0 71 2621.5 72 2033.5 73 1599.0 74 1237.5 75 1025.5 76 726.0 77 424.0 78 263.0 79 176.5 80 96.0 81 52.5 82 36.5 83 31.0 84 20.5 85 14.5 86 12.0 87 10.0 88 6.5 89 7.5 90 7.0 91 5.0 92 4.5 93 2.5 94 2.0 95 2.0 96 0.5 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.49113336468897645 2 0.04904243174829769 3 0.05664499345706995 4 0.06479903632606401 5 0.08110712206405217 6 0.11033562355580871 7 0.0694866261879495 8 0.05258766777829512 9 0.05042113464885225 10-11 0.07498174203444552 12-13 0.10850391827364338 14-15 0.05558142264807072 16-17 0.06007205495273413 18-19 0.05855548176212412 20-21 0.07573018075188942 22-23 0.06483842783750844 24-25 0.09075804236793404 26-27 0.06783218270728404 28-29 0.06775339968439521 30-31 0.07186981263033666 32-33 0.07185011687461446 34-35 0.0742333033170016 36-37 0.06928966863072743 38-39 0.04041569074197063 40-41 0.043547315901801685 42-43 0.04163682759674752 44-45 0.021704722805873117 46-47 0.0286376288200903 48-49 0.02808614765986848 50-51 0.0261362678433699 52-53 0.025368133370203785 54-55 0.024639390408482096 56-57 0.025112088545815082 58-59 0.02158654827153987 60-61 0.027830102835479778 62-63 0.019538189676430246 64-65 0.022078942164595067 66-67 0.015599038531988666 68-69 0.018218574043042314 70-71 0.04138078277235882 72-73 0.03783554674236139 74-75 0.03180864549136578 76-77 0.022394074256150392 78-79 0.014200639875711903 80-81 0.016386868760876982 82-83 0.02111385013420688 84-85 0.01780496317287595 86-87 0.009591833036715252 88-89 0.04169591486391415 90-91 0.05331641074001681 92-93 0.034388789490975014 94-95 0.05396637067884968 96-97 0.04419727584063455 98-99 0.03397517862080864 100-101 0.16893049682937725 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2538618.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.936195500062496 #Duplication Level Percentage of deduplicated Percentage of total 1 75.51745174137997 39.97606589048225 2 13.294198355864326 14.07488566365287 3 4.484149174925534 7.121213921259062 4 2.110962457033356 4.469852852752401 5 1.1871990585360972 3.1422900730078496 6 0.733257382262873 2.3289513683598906 7 0.48851180604306144 1.8101969528148882 8 0.3573803272331422 1.5134683896231942 9 0.2696438325143755 1.2846526770030384 >10 1.412145532058128 13.424971306534687 >50 0.0927218541133874 3.391313954778947 >100 0.047537502368855136 4.444740638342293 >500 0.0035003977898857793 1.2083384013578364 >1k 0.0011171482308101794 0.7825084208318182 >5k 1.4895309744135726E-4 0.57998193162318 >10k+ 7.447654872067863E-5 0.44656755757567534 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11327 0.44618765013089795 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9133 0.3597626740218497 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5578 0.21972585083695145 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.969575572220791E-5 0.0 16-17 0.0 0.0 0.0 5.908726716662373E-5 1.969575572220791E-5 18-19 0.0 0.0 0.0 7.878302288883164E-5 3.939151144441582E-5 20-21 0.0 0.0 0.0 9.847877861103955E-5 3.939151144441582E-5 22-23 0.0 0.0 0.0 2.3634906866649492E-4 3.939151144441582E-5 24-25 0.0 0.0 0.0 4.530023816107819E-4 3.939151144441582E-5 26-27 0.0 0.0 0.0 0.0010044835418326033 3.939151144441582E-5 28-29 0.0 0.0 0.0 0.00386036812155275 3.939151144441582E-5 30-31 0.0 0.0 0.0 0.01290071999804618 3.939151144441582E-5 32-33 0.0 0.0 0.0 0.03172986246847694 3.939151144441582E-5 34-35 0.0 0.0 0.0 0.06095836396023348 3.939151144441582E-5 36-37 0.0 0.0 0.0 0.11124162831903027 3.939151144441582E-5 38-39 0.0 0.0 0.0 0.18582945523903163 3.939151144441582E-5 40-41 0.0 0.0 0.0 0.26309590493725327 3.939151144441582E-5 42-43 0.0 0.0 0.0 0.3398699607424197 3.939151144441582E-5 44-45 0.0 0.0 0.0 0.4352565057050726 3.939151144441582E-5 46-47 0.0 0.0 0.0 0.5466950915813249 3.939151144441582E-5 48-49 0.0 0.0 0.0 0.6628606588309072 3.939151144441582E-5 50-51 0.0 0.0 0.0 0.7884014058042604 3.939151144441582E-5 52-53 0.0 0.0 0.0 0.9065562443817856 3.939151144441582E-5 54-55 0.0 0.0 0.0 1.0351695292478034 3.939151144441582E-5 56-57 0.0 0.0 0.0 1.181469602752364 3.939151144441582E-5 58-59 0.0 0.0 0.0 1.3402764811405259 3.939151144441582E-5 60-61 3.939151144441582E-5 0.0 0.0 1.5088721501226257 3.939151144441582E-5 62-63 3.939151144441582E-5 0.0 0.0 1.6673245049077883 3.939151144441582E-5 64-65 3.939151144441582E-5 0.0 0.0 1.8326506784400016 3.939151144441582E-5 66-67 3.939151144441582E-5 0.0 0.0 2.017121126534201 3.939151144441582E-5 68-69 3.939151144441582E-5 0.0 0.0 2.2205585873888865 3.939151144441582E-5 70-71 3.939151144441582E-5 0.0 0.0 2.435911980455508 3.939151144441582E-5 72-73 3.939151144441582E-5 0.0 0.0 2.6532152533386277 3.939151144441582E-5 74-75 3.939151144441582E-5 0.0 0.0 2.866323330252917 3.939151144441582E-5 76-77 3.939151144441582E-5 0.0 0.0 3.1016876111333014 3.939151144441582E-5 78-79 3.939151144441582E-5 0.0 0.0 3.3593277917355033 3.939151144441582E-5 80-81 3.939151144441582E-5 0.0 0.0 3.6296717347785292 3.939151144441582E-5 82-83 3.939151144441582E-5 0.0 0.0 3.9024185600196644 3.939151144441582E-5 84-85 3.939151144441582E-5 0.0 0.0 4.174180597474689 3.939151144441582E-5 86-87 3.939151144441582E-5 0.0 0.0 4.460891713522869 3.939151144441582E-5 88-89 3.939151144441582E-5 0.0 0.0 4.76802732825498 3.939151144441582E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4600 0.0 56.658424 1 GTATCAA 8320 0.0 40.90683 1 ATCAACG 10555 0.0 31.518356 3 TATCAAC 10620 0.0 31.325447 2 TCAACGC 10670 0.0 31.134115 4 CAACGCA 10840 0.0 30.733534 5 AACGCAG 11125 0.0 29.903484 6 GTGGTAT 2045 0.0 27.54345 1 GTACATG 9570 0.0 27.233932 1 ACGCAGA 12905 0.0 25.640156 7 TACATGG 9910 0.0 25.417099 2 TGGTATC 2115 0.0 25.391703 2 CGCAGAG 13030 0.0 25.138285 8 ACATGGG 9955 0.0 24.251925 3 GAGTACT 8720 0.0 22.432173 12-13 GCAGAGT 14350 0.0 22.427921 9 GTACTTT 9160 0.0 20.978348 14-15 CATGGGG 5275 0.0 20.541689 4 CAGAGTA 14035 0.0 20.474972 10-11 AGTACTT 9075 0.0 20.114193 12-13 >>END_MODULE