FastQCFastQC Report
Thu 26 May 2016
SRR938013_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938013_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2538618
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109970.4331884513542408No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96920.3817825289192781No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59450.23418253553705204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49950.054.501311
GTATCAA87000.038.9912151
ATCAACG104650.032.0928343
TATCAAC107000.031.6593592
TCAACGC107400.031.3558484
CAACGCA108750.031.0102795
AACGCAG111400.030.3578786
GTACATG100600.026.9665831
GTGGTAT23100.026.7374021
TACATGG102700.026.1381232
ACGCAGA129100.025.974977
CGCAGAG130150.025.6559288
ACATGGG101650.025.5160333
TGGTATC23600.024.9635312
TATTACG1151.2802919E-424.7884732
GCAGAGT145250.022.8902179
CATGGGG52400.022.7519724
GAGTACT86950.022.6697412-13
GTACTTT88750.021.91561114-15
GAGTACA79550.021.2616831
Produced by FastQC (version 0.11.5)