##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938013_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2538618 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.545984074799755 34.0 31.0 34.0 31.0 34.0 2 32.76273113954128 34.0 31.0 34.0 31.0 34.0 3 32.821428430744604 34.0 31.0 34.0 31.0 34.0 4 36.26327592414455 37.0 37.0 37.0 35.0 37.0 5 36.22798625078685 37.0 37.0 37.0 35.0 37.0 6 36.26787764051149 37.0 37.0 37.0 35.0 37.0 7 36.22991801050808 37.0 37.0 37.0 35.0 37.0 8 36.22528438701687 37.0 37.0 37.0 35.0 37.0 9 37.94613210809976 39.0 38.0 39.0 35.0 39.0 10-11 37.93273564593019 39.0 38.0 39.0 35.0 39.0 12-13 37.881823102176064 39.0 38.0 39.0 35.0 39.0 14-15 39.232318529215505 41.0 39.0 41.0 36.0 41.0 16-17 39.183826199924525 40.0 39.0 41.0 36.0 41.0 18-19 39.2002130686854 40.0 39.0 41.0 36.0 41.0 20-21 39.133003665774055 40.0 39.0 41.0 36.0 41.0 22-23 38.99264639264356 40.0 38.5 41.0 35.0 41.0 24-25 38.95192640247568 40.0 38.0 41.0 35.0 41.0 26-27 38.93211759311562 40.0 38.0 41.0 35.0 41.0 28-29 38.8818266474121 40.0 38.0 41.0 35.0 41.0 30-31 38.70531781465348 40.0 38.0 41.0 35.0 41.0 32-33 38.55639603910474 40.0 38.0 41.0 34.5 41.0 34-35 38.37112870073402 40.0 38.0 41.0 34.0 41.0 36-37 38.25877150481088 40.0 38.0 41.0 34.0 41.0 38-39 38.25570586043273 40.0 38.0 41.0 34.0 41.0 40-41 38.20259270989176 40.0 38.0 41.0 34.0 41.0 42-43 38.06976453330119 40.0 38.0 41.0 33.5 41.0 44-45 37.876344924679486 40.0 38.0 41.0 33.0 41.0 46-47 37.57812026070878 40.0 37.0 41.0 33.0 41.0 48-49 37.266404004068356 40.0 37.0 41.0 32.0 41.0 50-51 36.9944201924039 40.0 36.5 41.0 31.0 41.0 52-53 36.600255335777184 39.0 36.0 41.0 31.0 41.0 54-55 36.3873737600537 39.0 35.0 41.0 30.0 41.0 56-57 36.16203501275103 39.0 35.0 41.0 30.0 41.0 58-59 35.83876168056793 38.5 35.0 40.0 29.0 41.0 60-61 35.49615952459173 38.0 35.0 40.0 28.0 41.0 62-63 35.1381550906832 38.0 34.5 40.0 28.0 41.0 64-65 34.756623682649376 37.0 34.0 40.0 28.0 41.0 66-67 34.349671947492695 36.5 34.0 39.0 26.5 41.0 68-69 33.90222888989206 36.0 33.5 39.0 26.0 40.5 70-71 33.39475100231701 35.5 33.0 38.5 26.0 40.0 72-73 32.98059593842004 35.0 33.0 37.5 25.0 40.0 74-75 32.53500034270615 35.0 32.0 37.0 24.5 39.0 76-77 31.221426579343564 34.0 30.5 35.5 23.0 38.5 78-79 31.937610345471434 35.0 32.0 36.0 24.0 38.5 80-81 31.76840647155263 35.0 32.0 36.0 24.0 37.5 82-83 31.414685667556128 35.0 32.0 35.0 22.5 37.0 84-85 31.04712583775897 34.5 31.0 35.0 20.5 36.5 86-87 30.62013564073051 34.0 31.0 35.0 19.0 36.0 88-89 30.179353096842455 34.0 31.0 35.0 17.5 36.0 90-91 29.844271765188775 34.0 30.0 35.0 12.5 35.0 92-93 29.449674586723958 34.0 30.0 35.0 4.5 35.0 94-95 29.05133442684169 34.0 29.5 35.0 2.0 35.0 96-97 28.496252094643623 34.0 29.0 35.0 2.0 35.0 98-99 27.82494589575903 34.0 29.0 35.0 2.0 35.0 100-101 25.40196083065668 31.0 22.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12474809545195598 1101 2 0.07954871019540377 1101 3 0.12398146308594704 1101 4 0.05475001217214981 1101 5 -0.021225106411463912 1101 6 0.007633454132466966 1101 7 0.03858012910740882 1101 8 0.0783623513023386 1101 9 0.0308216577457614 1101 10-11 0.08481409061018752 1101 12-13 0.15369600171053577 1101 14-15 0.15599558642755795 1101 16-17 0.1697355897713848 1101 18-19 0.12426495934199266 1101 20-21 0.16543053775257732 1101 22-23 0.22183054747461028 1101 24-25 0.16425573150367256 1101 26-27 0.14004261901978055 1101 28-29 0.14889390392583834 1101 30-31 0.13048683862905364 1101 32-33 -0.022373464847959212 1101 34-35 0.014537654972691882 1101 36-37 0.08265588635702414 1101 38-39 0.11350275513517971 1101 40-41 0.045096265457878815 1101 42-43 0.07626570325867732 1101 44-45 0.07937621219006274 1101 46-47 0.03605910096433007 1101 48-49 0.03551046493499399 1101 50-51 0.00942216395292661 1101 52-53 0.039330243114619634 1101 54-55 0.18526871407654966 1101 56-57 0.03629566144507379 1101 58-59 0.09539758429558987 1101 60-61 0.07505837047688146 1101 62-63 0.08681156790935063 1101 64-65 0.13653933851112043 1101 66-67 0.1225254496412802 1101 68-69 0.028868202923789 1101 70-71 -0.14008482577283843 1101 72-73 -0.2027665462807846 1101 74-75 0.038268457530435285 1101 76-77 -0.04737560751907566 1101 78-79 0.07094055041463676 1101 80-81 0.2546094706735289 1101 82-83 0.24963482532755066 1101 84-85 0.2683857808013741 1101 86-87 0.2982648799638561 1101 88-89 0.30371675670469145 1101 90-91 0.2889698553891762 1101 92-93 0.3045376850891266 1101 94-95 0.26613711604682067 1101 96-97 0.38321841992840433 1101 98-99 0.5715855014225468 1101 100-101 0.8025872805271632 1102 1 0.18767311822382027 1102 2 0.17585111759044736 1102 3 0.16331256119248394 1102 4 0.09410900168131064 1102 5 0.1024317941050441 1102 6 0.0925583712105933 1102 7 0.1534316037185235 1102 8 0.16588704069853577 1102 9 0.2000899928565616 1102 10-11 0.18693947053880322 1102 12-13 0.19749605485099408 1102 14-15 0.3208468958922097 1102 16-17 0.2859979656638245 1102 18-19 0.2990062482330771 1102 20-21 0.21121757808574415 1102 22-23 0.24149481205540013 1102 24-25 0.24451883917842565 1102 26-27 0.2042408292725142 1102 28-29 0.12662087571955283 1102 30-31 0.19354223535856363 1102 32-33 0.16067670958340585 1102 34-35 0.28101679477247643 1102 36-37 0.306417049340439 1102 38-39 0.296994421110675 1102 40-41 0.27651903159507896 1102 42-43 0.2548211414583079 1102 44-45 0.22376449868173864 1102 46-47 0.2188216453061571 1102 48-49 0.32284402482481056 1102 50-51 0.33128684000529773 1102 52-53 0.2477825124839228 1102 54-55 0.28634146880028766 1102 56-57 0.3614526239832543 1102 58-59 0.394043860389651 1102 60-61 0.3355468223352176 1102 62-63 0.3290056450966006 1102 64-65 0.40557879897395566 1102 66-67 0.4475473590720185 1102 68-69 0.5906727069449218 1102 70-71 0.6533625479213114 1102 72-73 0.4666662013086267 1102 74-75 0.5714061045383119 1102 76-77 0.5656662288508763 1102 78-79 0.41694743738912976 1102 80-81 0.47891699320501147 1102 82-83 0.504739777739676 1102 84-85 0.3909154649605995 1102 86-87 0.3209565544535735 1102 88-89 0.18821277006907522 1102 90-91 0.12649537051769144 1102 92-93 0.23812908117860232 1102 94-95 0.22111708928575524 1102 96-97 0.24856964608584775 1102 98-99 0.29715648614319434 1102 100-101 0.35000890385083494 1103 1 0.11084113514913696 1103 2 0.16972503586120524 1103 3 0.07046864882688908 1103 4 0.06263565495159895 1103 5 0.058243570739549 1103 6 0.09744465068510522 1103 7 0.11336319155756058 1103 8 0.09135729464912146 1103 9 0.09415982962183023 1103 10-11 0.050011949760957464 1103 12-13 0.0705106719142421 1103 14-15 0.12984990653709616 1103 16-17 0.0936625775582769 1103 18-19 0.16604117369625015 1103 20-21 0.16782697735871466 1103 22-23 0.18320941396628854 1103 24-25 0.15193177333933505 1103 26-27 0.13188675562641805 1103 28-29 0.14969050856532817 1103 30-31 0.18587454342994647 1103 32-33 0.24081353390533167 1103 34-35 0.23127831445424363 1103 36-37 0.21721536587165957 1103 38-39 0.1895359236858667 1103 40-41 0.1664957861878662 1103 42-43 0.1390731038294959 1103 44-45 0.23221193488963365 1103 46-47 0.2646042288812893 1103 48-49 0.32613470499547503 1103 50-51 0.34573804164345034 1103 52-53 0.2112908342405717 1103 54-55 0.28341225010936455 1103 56-57 0.34036306367052305 1103 58-59 0.2890157577331749 1103 60-61 0.34211118938466 1103 62-63 0.4274506172771666 1103 64-65 0.3994298825087341 1103 66-67 0.30999462676309264 1103 68-69 0.38592991457886683 1103 70-71 0.4237175023863742 1103 72-73 0.3042358207013649 1103 74-75 0.19915653963739288 1103 76-77 0.32690304311844187 1103 78-79 0.3411762959638729 1103 80-81 0.37468421201205615 1103 82-83 0.48562427150243437 1103 84-85 0.44868309868718725 1103 86-87 0.46043360005648637 1103 88-89 0.488227922421018 1103 90-91 0.4782627082777786 1103 92-93 0.6861634175409925 1103 94-95 0.6186570978673664 1103 96-97 0.6796039640388329 1103 98-99 0.641748937439715 1103 100-101 0.786399472007389 1104 1 0.13427587892832804 1104 2 0.08332220703996995 1104 3 0.060378675699887197 1104 4 0.022151443760364486 1104 5 0.024434936840599164 1104 6 -0.015828821522276826 1104 7 0.03553667189605392 1104 8 0.03465923919493008 1104 9 0.0815512271721488 1104 10-11 0.08702867767978262 1104 12-13 0.07626713094182946 1104 14-15 0.11964485462900143 1104 16-17 0.04331804680754914 1104 18-19 0.1142910018340686 1104 20-21 0.11748328659908935 1104 22-23 0.1822750078635167 1104 24-25 0.10359582931365452 1104 26-27 0.09949233074816988 1104 28-29 0.10994884950897443 1104 30-31 0.12865703747777957 1104 32-33 0.023184439032711168 1104 34-35 0.13711665581453047 1104 36-37 0.12531966723089738 1104 38-39 0.06321352569271 1104 40-41 0.04071350247381389 1104 42-43 -0.03897023433546565 1104 44-45 -0.054091472584673284 1104 46-47 -0.035127948355487604 1104 48-49 0.07097346366334278 1104 50-51 -0.00451607246497332 1104 52-53 0.08461194402337924 1104 54-55 -2.0419382526171148E-4 1104 56-57 0.024434345987494055 1104 58-59 0.10385416696474437 1104 60-61 -0.026779807321901217 1104 62-63 -0.05056127162090718 1104 64-65 0.04895905069577822 1104 66-67 0.06278070925291246 1104 68-69 0.1580771199956672 1104 70-71 0.014286687077564864 1104 72-73 -0.051179702737400135 1104 74-75 -0.09488671385646796 1104 76-77 -0.03172242232063027 1104 78-79 0.018894053577387382 1104 80-81 0.06496504455747498 1104 82-83 0.24394488737047126 1104 84-85 0.2551002611165991 1104 86-87 0.01912999434427931 1104 88-89 0.0750105952189486 1104 90-91 0.10885242907145809 1104 92-93 0.14962447656145272 1104 94-95 0.11092027581578634 1104 96-97 0.14060989706398885 1104 98-99 0.26841808376011755 1104 100-101 0.4017359623632828 1105 1 0.12513688158623637 1105 2 0.11538306115969021 1105 3 0.12961919719343484 1105 4 0.0370558859765282 1105 5 0.06576512081225161 1105 6 0.07362487301919174 1105 7 -0.0018160908858817493 1105 8 0.06644873757041125 1105 9 0.019189243640262532 1105 10-11 0.07564627895159504 1105 12-13 0.0937125854501275 1105 14-15 0.10671686011774995 1105 16-17 0.12549591719635345 1105 18-19 0.11152509128461219 1105 20-21 0.16771373460019134 1105 22-23 0.2171259838795052 1105 24-25 0.1703629416263226 1105 26-27 0.18459725863765897 1105 28-29 0.08315687182869169 1105 30-31 0.2711082696938334 1105 32-33 0.3622650587299887 1105 34-35 0.32081356811317363 1105 36-37 0.30048152951738416 1105 38-39 0.1628149974910471 1105 40-41 0.26527523779223117 1105 42-43 0.2941308115632708 1105 44-45 0.24253400020476334 1105 46-47 0.17676834406979225 1105 48-49 0.2127840384878894 1105 50-51 0.24029593900429091 1105 52-53 0.3271413285803675 1105 54-55 0.3164643437182022 1105 56-57 0.23146596569200995 1105 58-59 0.2590731114317677 1105 60-61 0.31524767915983176 1105 62-63 0.43294325480043483 1105 64-65 0.4753347818705933 1105 66-67 0.46228892020102563 1105 68-69 0.42636800643596473 1105 70-71 0.3571220307387719 1105 72-73 0.29605251583635095 1105 74-75 0.3920981936703356 1105 76-77 0.41897352812723554 1105 78-79 0.41834165755018304 1105 80-81 0.4571969597033103 1105 82-83 0.5009796985707027 1105 84-85 0.520822858195455 1105 86-87 0.49998078421588943 1105 88-89 0.45997761276148097 1105 90-91 0.5048604496303462 1105 92-93 0.6584300962351541 1105 94-95 0.6537330117388507 1105 96-97 0.7677376263823916 1105 98-99 0.9679101485697998 1105 100-101 1.1385999703725211 1106 1 -0.11236898317613253 1106 2 -0.05249919729158847 1106 3 -0.012441912813315525 1106 4 -0.06573739002698886 1106 5 4.748273319634677E-4 1106 6 -0.013206592355750502 1106 7 0.03681690766200063 1106 8 -0.06503425766438653 1106 9 -0.06739737997703799 1106 10-11 -0.012514284945119414 1106 12-13 -0.0761482410274894 1106 14-15 -0.16609011989289257 1106 16-17 -0.14717631070616477 1106 18-19 -0.07924229448585862 1106 20-21 -0.029723137677095224 1106 22-23 -0.01999961845647391 1106 24-25 -0.00933649728789021 1106 26-27 -0.04402484682911734 1106 28-29 0.023123173401828012 1106 30-31 -0.04327374703563436 1106 32-33 -0.04249573176421251 1106 34-35 0.07111642607613078 1106 36-37 -0.07249945453848738 1106 38-39 -0.07586976250609467 1106 40-41 0.0472686122078656 1106 42-43 -0.062492945396819266 1106 44-45 0.016259582513633575 1106 46-47 -0.09308872361142306 1106 48-49 -0.15690695873417582 1106 50-51 -0.13988232302928338 1106 52-53 -0.27067540498693177 1106 54-55 -0.5160272717887153 1106 56-57 -0.40233916344567433 1106 58-59 -0.22892020831046267 1106 60-61 -0.25175762089165943 1106 62-63 -0.2037198967230438 1106 64-65 -0.2560308735461305 1106 66-67 -0.2366548877241783 1106 68-69 -0.46376482616031467 1106 70-71 -0.41848101837310736 1106 72-73 -0.2697608149059434 1106 74-75 -0.4434430982680979 1106 76-77 -0.23983376268980194 1106 78-79 -0.27086010780642766 1106 80-81 -0.5916797990197011 1106 82-83 -0.5707048572925899 1106 84-85 -0.4423870677607127 1106 86-87 -0.2085798225875628 1106 88-89 -0.06153800526041309 1106 90-91 -0.12084335899610466 1106 92-93 -0.001879970297029132 1106 94-95 -0.07086718157916749 1106 96-97 0.14345198340981113 1106 98-99 0.24272659896453774 1106 100-101 0.1178300945851447 1107 1 0.0555679612622626 1107 2 0.06340350834566522 1107 3 0.045399201007164436 1107 4 0.04825288374368597 1107 5 -0.009852991951674994 1107 6 0.06886791660825509 1107 7 0.03213790644450398 1107 8 0.022838249932078725 1107 9 0.016084465807480797 1107 10-11 0.021613275033487866 1107 12-13 0.07896747842370644 1107 14-15 0.13295979174411343 1107 16-17 0.13629353763933238 1107 18-19 0.10727364349625645 1107 20-21 0.09116580669716257 1107 22-23 0.07965271351352499 1107 24-25 0.14063349479781095 1107 26-27 0.16924852985152938 1107 28-29 0.15913762415964072 1107 30-31 0.13483553674875282 1107 32-33 0.2145459632019069 1107 34-35 0.25352202427016124 1107 36-37 0.22471333135275273 1107 38-39 0.2128202603205409 1107 40-41 0.2737331587733891 1107 42-43 0.2872312566732731 1107 44-45 0.35061174371340087 1107 46-47 0.3204083093846819 1107 48-49 0.3673340242884464 1107 50-51 0.41502042040419695 1107 52-53 0.3606936889229573 1107 54-55 0.4519358436741854 1107 56-57 0.40920068351395855 1107 58-59 0.28587658542407013 1107 60-61 0.36628815950309246 1107 62-63 0.38578815673686506 1107 64-65 0.4051899388895137 1107 66-67 0.412769922407044 1107 68-69 0.604123498584876 1107 70-71 0.5179820907904187 1107 72-73 0.3618518069725738 1107 74-75 0.39577160492270735 1107 76-77 0.436785290274166 1107 78-79 0.5449283787753352 1107 80-81 0.6171976186503798 1107 82-83 0.5262436127046506 1107 84-85 0.6002447866711229 1107 86-87 0.6808746575825886 1107 88-89 0.6715249895981508 1107 90-91 0.6306939739417032 1107 92-93 0.6204934781964475 1107 94-95 0.6805016431757593 1107 96-97 0.777862814663397 1107 98-99 0.7771467933745875 1107 100-101 0.9054671202281668 1108 1 0.051445022731527956 1108 2 0.03304368825570947 1108 3 0.05739320400566328 1108 4 0.049752134118499214 1108 5 -0.02072255716906568 1108 6 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#Quality Count 2 7.0 3 4.0 4 13.0 5 41.0 6 76.0 7 206.0 8 439.0 9 825.0 10 1460.0 11 2321.0 12 3520.0 13 5360.0 14 7383.0 15 9886.0 16 12816.0 17 18536.0 18 18864.0 19 13059.0 20 11244.0 21 11379.0 22 11793.0 23 12930.0 24 14968.0 25 17587.0 26 20990.0 27 25713.0 28 31863.0 29 39231.0 30 48576.0 31 59907.0 32 75639.0 33 99017.0 34 139237.0 35 212028.0 36 354634.0 37 612122.0 38 574822.0 39 70112.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.10706927942684 18.436606058887158 13.973469029211957 23.48285563247405 2 15.98815861219061 19.563149865890058 39.936790249529594 24.511901272389743 3 19.805725343002155 23.387019257284315 29.344470759528974 27.46278464018455 4 13.099568347817591 16.058028423339 35.79569671372377 35.04670651511964 5 14.018262699729814 37.12340223042704 33.85552054524176 15.002814524601387 6 33.61561290434402 35.189894659220094 16.68541702611421 14.509075410321678 7 29.784867199397468 32.19180672318561 19.609803444236196 18.413522633180733 8 26.98390063853841 35.417886166051495 18.773895950933777 18.82431724447631 9 26.509903466705744 16.573032601356807 19.639952005155585 37.27711192678186 10-11 25.704792922763488 26.289362952598616 26.467648933396042 21.538195191241847 12-13 26.1926764877583 23.401039463203997 27.459566583077876 22.946717465959825 14-15 22.842073915807735 24.98680384366612 25.493496855375643 26.677625385150506 16-17 22.73135244654507 28.47886668711092 26.177588093959276 22.612192772384734 18-19 22.589475848670418 26.695883350704992 28.651494632118734 22.063146168505856 20-21 22.945752373929437 25.85063999388644 29.029928882565237 22.173678749618887 22-23 22.853792890462447 25.752771783702787 28.933498462549306 22.459936863285456 24-25 22.659429507424345 26.008017418345595 28.95817682520161 22.37437624902845 26-27 22.25937647442395 26.467794887251817 28.78642904493281 22.48639959339143 28-29 22.277843069246575 26.560569507052367 28.877540187736116 22.284047235964945 30-31 22.39275464051701 26.246977686284428 28.809572767545177 22.55069490565339 32-33 22.105883265901337 26.310118108080534 28.839083796743648 22.744914829274478 34-35 22.718711456484648 26.37779554682469 28.65351402602279 22.249978970667875 36-37 22.660218544922095 26.156334584326203 28.551374680571374 22.63207219018033 38-39 22.32847811012702 26.368944432187096 28.710502883978943 22.592074573706938 40-41 22.617222188515232 26.23238229715855 28.675694799571666 22.474700714754547 42-43 22.50158318699728 26.229927010893704 28.571054318825794 22.697435483283222 44-45 22.364664227992648 26.416130071718445 28.591713025371295 22.627492674917608 46-47 22.54234834511304 26.628351848988675 28.1039778540879 22.725321951810386 48-49 22.60869890625529 26.671401526342287 28.007088896399534 22.712810671002885 50-51 22.594439179112417 26.85449327153593 27.89602452988201 22.655043019469648 52-53 22.88438039909904 26.669313776235732 27.848538062835765 22.597767761829466 54-55 22.442801555807137 26.64323659565953 28.006734372796537 22.9072274757368 56-57 22.514966804773305 27.022143544243367 27.823209320977004 22.639680330006325 58-59 22.663413715651586 26.620921304426265 27.80668458192607 22.908980397996075 60-61 22.509786821018366 26.662105129641407 28.116991213329456 22.711116836010774 62-63 22.522273930146245 26.770963571518045 28.0454562285464 22.66130626978931 64-65 22.252599642797772 26.68668543278272 28.01699586152781 23.0437190628917 66-67 22.240053446402726 26.867393991533977 27.872271448481023 23.02028111358227 68-69 22.4048678540471 26.719900449595812 27.91037312296653 22.964858573390554 70-71 22.26112790502549 26.88356420698191 27.714390270611805 23.140917617380797 72-73 22.439669930647305 26.875095032021363 27.66920820698506 23.016026830346277 74-75 22.28232053818259 27.03226716268458 27.565175225260358 23.120237073872477 76-77 22.349502760951037 26.849805681674045 27.630407568212313 23.170283989162606 78-79 22.41026416735405 27.01637268781676 27.59668843441589 22.976674710413302 80-81 22.352949518202422 27.25071278939959 27.431263782105063 22.965073910292926 82-83 22.34751789113563 27.19413223929954 27.416064058488526 23.042285811076304 84-85 22.37428226573973 27.102661998373872 27.335610491683422 23.187445244202976 86-87 22.403005578834893 27.063622352761595 27.36416054045271 23.1692115279508 88-89 22.29146375930994 27.146192760429642 27.29436395991125 23.267979520349165 90-91 22.28405387429004 27.2098726332307 27.392807136651566 23.1132663558277 92-93 22.43238324610052 26.92630097821287 27.438707575684163 23.20260820000244 94-95 22.231328226617787 26.823649718074954 27.46707066600804 23.477951389299218 96-97 22.38629443264012 26.69383499210988 27.42728523944918 23.49258533580082 98-99 22.19457594644015 26.82575716393723 27.178803585257803 23.800863304364817 100-101 22.124695405137757 26.7332265035543 27.498780832720794 23.643297258587154 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1105.0 1 793.0 2 693.5 3 923.5 4 736.5 5 640.5 6 1316.0 7 2760.5 8 4321.0 9 5037.5 10 4149.5 11 3007.5 12 2800.5 13 2883.0 14 3028.0 15 3147.0 16 3258.0 17 3355.0 18 3514.0 19 3651.5 20 4025.5 21 4749.0 22 5599.0 23 6731.5 24 8346.5 25 10517.0 26 13052.5 27 16056.0 28 18930.5 29 21813.5 30 25552.0 31 29639.0 32 34999.0 33 38666.0 34 41405.0 35 45362.5 36 49993.0 37 55529.5 38 59695.5 39 62359.0 40 66056.5 41 71655.0 42 76609.5 43 81979.5 44 87931.0 45 93310.5 46 95359.5 47 97423.0 48 102469.0 49 103849.5 50 103115.0 51 100670.5 52 96056.5 53 94479.0 54 93828.5 55 87784.5 56 79011.0 57 71545.5 58 64828.5 59 56953.0 60 47076.0 61 37493.0 62 29868.0 63 23044.5 64 17652.5 65 13974.0 66 10498.5 67 8275.0 68 6158.5 69 4031.5 70 2892.0 71 2237.5 72 1805.5 73 1478.0 74 1184.5 75 985.0 76 652.5 77 345.0 78 201.0 79 122.0 80 54.0 81 28.5 82 17.0 83 12.5 84 9.0 85 6.0 86 3.5 87 2.0 88 1.5 89 1.5 90 1.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01583538760065516 3 0.005908726716662373 4 0.0 5 3.939151144441582E-5 6 0.0 7 0.0 8 3.1513209155532656E-4 9 4.726981373329898E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.9543633583311863E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005711769159440294 26-27 0.02168502705015091 28-29 5.908726716662372E-5 30-31 0.0 32-33 4.33306625888574E-4 34-35 0.019557885432152453 36-37 0.003978542655885997 38-39 0.018041312241542446 40-41 0.01591417062354399 42-43 0.008882785830715768 44-45 0.0031513209155532656 46-47 1.969575572220791E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.7726180149987117E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.969575572220791E-5 84-85 0.002914971846886771 86-87 2.16653312944287E-4 88-89 1.969575572220791E-5 90-91 1.7726180149987117E-4 92-93 3.5452360299974235E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2538618.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.022391627638555 #Duplication Level Percentage of deduplicated Percentage of total 1 73.89554577230054 37.70327475932408 2 14.026819388610162 14.313637442716423 3 4.900723080555702 7.501398368241608 4 2.253436270156353 4.599028315353702 5 1.294324101555229 3.301975560132115 6 0.7770372149833639 2.3787778255278456 7 0.5286932554147893 1.888263603006516 8 0.359651383111085 1.468021897481252 9 0.28481432746038193 1.3078717341161488 >10 1.519227590165484 14.074758848198716 >50 0.10309352053815854 3.63932683624162 >100 0.051381584267718494 4.683898512694421 >500 0.004016625967950646 1.3546786866045628 >1k 0.0010041564919849788 0.7354116397168805 >5k 1.5448561415153518E-4 0.6162718011925434 >10k+ 7.724280707576759E-5 0.4334041694515828 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10997 0.4331884513542408 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9692 0.3817825289192781 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5945 0.23418253553705204 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.969575572220791E-5 0.0 16-17 0.0 0.0 0.0 5.908726716662373E-5 0.0 18-19 0.0 0.0 0.0 7.878302288883164E-5 0.0 20-21 0.0 0.0 0.0 9.847877861103955E-5 0.0 22-23 0.0 0.0 0.0 1.969575572220791E-4 0.0 24-25 0.0 0.0 0.0 4.136108701663661E-4 0.0 26-27 0.0 0.0 0.0 0.0010044835418326033 0.0 28-29 0.0 0.0 0.0 0.003899759632997166 0.0 30-31 0.0 0.0 0.0 0.012940111509490596 0.0 32-33 0.0 0.0 0.0 0.031769253979921364 0.0 34-35 0.0 0.0 0.0 0.061214408784622185 0.0 36-37 0.0 0.0 0.0 0.11185219674641872 0.0 38-39 0.0 0.0 0.0 0.18714907087241955 0.0 40-41 0.0 0.0 0.0 0.26508517626519623 0.0 42-43 0.0 0.0 0.0 0.3423516259634179 0.0 44-45 0.0 0.0 0.0 0.43805330301762613 0.0 46-47 0.0 0.0 0.0 0.5497085422068229 0.0 48-49 0.0 0.0 0.0 0.6664255906166268 0.0 50-51 0.0 0.0 0.0 0.7929511253760905 0.0 52-53 0.0 0.0 0.0 0.9117362281367264 0.0 54-55 0.0 0.0 0.0 1.040526774804244 0.0 56-57 0.0 0.0 0.0 1.187102588888915 0.0 58-59 0.0 0.0 0.0 1.3470518211089657 0.0 60-61 0.0 0.0 0.0 1.517321629327453 0.0 62-63 0.0 0.0 0.0 1.6769360337002257 0.0 64-65 0.0 0.0 0.0 1.8433454737971604 0.0 66-67 0.0 0.0 0.0 2.028761318166026 0.0 68-69 0.0 0.0 0.0 2.232966913493877 0.0 70-71 0.0 0.0 0.0 2.4493444858580533 0.0 72-73 0.0 0.0 0.0 2.6685779428019494 0.0 74-75 0.0 0.0 0.0 2.88347833348696 3.939151144441582E-5 76-77 0.0 0.0 0.0 3.1203985790693993 3.939151144441582E-5 78-79 0.0 0.0 0.0 3.3801659012895993 3.939151144441582E-5 80-81 0.0 0.0 0.0 3.653799035538234 3.939151144441582E-5 82-83 0.0 0.0 0.0 3.9307607525039217 3.939151144441582E-5 84-85 0.0 0.0 0.0 4.205811981164555 5.908726716662373E-5 86-87 0.0 0.0 0.0 4.495418373303901 7.878302288883164E-5 88-89 0.0 0.0 0.0 4.809644460096004 7.878302288883164E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4995 0.0 54.50131 1 GTATCAA 8700 0.0 38.991215 1 ATCAACG 10465 0.0 32.092834 3 TATCAAC 10700 0.0 31.659359 2 TCAACGC 10740 0.0 31.355848 4 CAACGCA 10875 0.0 31.010279 5 AACGCAG 11140 0.0 30.357878 6 GTACATG 10060 0.0 26.966583 1 GTGGTAT 2310 0.0 26.737402 1 TACATGG 10270 0.0 26.138123 2 ACGCAGA 12910 0.0 25.97497 7 CGCAGAG 13015 0.0 25.655928 8 ACATGGG 10165 0.0 25.516033 3 TGGTATC 2360 0.0 24.963531 2 TATTACG 115 1.2802919E-4 24.788473 2 GCAGAGT 14525 0.0 22.890217 9 CATGGGG 5240 0.0 22.751972 4 GAGTACT 8695 0.0 22.66974 12-13 GTACTTT 8875 0.0 21.915611 14-15 GAGTACA 7955 0.0 21.261683 1 >>END_MODULE