FastQCFastQC Report
Thu 26 May 2016
SRR938012_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938012_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2622776
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110600.4216906056788685No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91870.35027772101010535No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55670.21225602186385725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50650.053.9641081
GTATCAA91650.040.5780451
ATCAACG110350.033.2757153
TATCAAC112750.032.7348982
TCAACGC113250.032.381624
CAACGCA115550.031.737075
AACGCAG118500.031.1004146
GTGGTAT24150.028.1962571
GTACATG101800.027.3172911
ACGCAGA134700.026.9634697
CGCAGAG136700.026.4626278
TGGTATC24250.026.0858922
TACATGG105400.025.67662
ACATGGG105350.024.1547013
GCAGAGT149300.023.719479
GAGTACT90450.022.43982912-13
TACACCG3059.094947E-1221.8328595
GTACTTT93450.021.69132214-15
CAGAGTA144100.021.27527610-11
CATGGGG56900.021.2326354
Produced by FastQC (version 0.11.5)