##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938012_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2622776 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.963221029931645 33.0 31.0 34.0 30.0 34.0 2 32.072283336434374 33.0 31.0 34.0 30.0 34.0 3 32.09923988933863 34.0 31.0 34.0 30.0 34.0 4 35.61246709593195 37.0 35.0 37.0 33.0 37.0 5 35.61948790136863 37.0 35.0 37.0 33.0 37.0 6 35.65095265474444 37.0 35.0 37.0 33.0 37.0 7 35.57832464533761 37.0 35.0 37.0 33.0 37.0 8 35.57197069059653 37.0 35.0 37.0 33.0 37.0 9 37.205880334424286 39.0 37.0 39.0 34.0 39.0 10-11 37.11043832946466 39.0 37.0 39.0 33.5 39.0 12-13 36.946570351413925 39.0 37.0 39.0 33.0 39.0 14-15 38.13048826891812 40.0 38.0 41.0 33.0 41.0 16-17 37.94670684801142 40.0 38.0 41.0 33.0 41.0 18-19 38.12001901801755 40.0 38.0 41.0 33.0 41.0 20-21 38.12408817985219 40.0 38.0 41.0 33.0 41.0 22-23 38.005793861160846 40.0 38.0 41.0 33.0 41.0 24-25 37.880873547721954 40.0 38.0 41.0 33.0 41.0 26-27 37.69997437829231 40.0 38.0 41.0 32.0 41.0 28-29 37.516157498772294 40.0 37.0 41.0 32.0 41.0 30-31 37.3058471253359 40.0 37.0 41.0 31.5 41.0 32-33 37.02637453598783 40.0 36.5 41.0 31.0 41.0 34-35 36.79182362504461 39.0 36.0 41.0 30.0 41.0 36-37 36.53069305194191 39.0 35.5 41.0 30.0 41.0 38-39 36.23545949025002 39.0 35.0 40.5 30.0 41.0 40-41 36.12828583150067 39.0 35.0 40.5 29.5 41.0 42-43 35.91625075873807 39.0 35.0 40.5 28.5 41.0 44-45 35.58888730871412 39.0 35.0 40.0 27.0 41.0 46-47 35.211472500892185 38.0 35.0 40.0 25.5 41.0 48-49 34.81898721049758 38.0 34.0 40.0 24.0 41.0 50-51 34.09043547752458 37.5 33.5 39.5 23.5 40.5 52-53 34.159356346100466 38.0 33.5 40.0 24.0 40.5 54-55 34.23045372536579 38.0 34.0 40.0 23.0 41.0 56-57 33.91072207462628 38.0 33.0 40.0 22.0 41.0 58-59 33.492340939523615 37.5 33.0 40.0 20.0 41.0 60-61 32.97480665523857 37.0 32.0 40.0 17.0 41.0 62-63 32.40645655595445 36.0 31.5 39.0 12.5 41.0 64-65 31.84203835935665 35.5 31.0 39.0 8.0 40.0 66-67 31.241476397526895 35.0 30.5 38.5 4.5 40.0 68-69 30.54508658002056 35.0 29.0 38.0 2.0 40.0 70-71 29.92073322311932 34.5 29.0 37.0 2.0 39.5 72-73 29.193693247154922 34.0 28.0 36.5 2.0 39.0 74-75 28.49262384587933 34.0 26.5 36.0 2.0 39.0 76-77 27.71535560032576 33.5 26.0 35.5 2.0 38.0 78-79 26.917139511723455 33.0 24.5 35.0 2.0 37.0 80-81 26.151023381333367 32.0 22.0 35.0 2.0 36.5 82-83 25.385402718341176 31.5 19.5 35.0 2.0 36.0 84-85 24.61590372185806 31.0 15.5 34.0 2.0 35.5 86-87 23.822639447669186 31.0 7.5 34.0 2.0 35.0 88-89 22.908300403846916 30.0 2.0 34.0 2.0 35.0 90-91 21.96635587636916 29.0 2.0 34.0 2.0 35.0 92-93 20.97290447220807 29.0 2.0 34.0 2.0 35.0 94-95 19.84428845620061 26.5 2.0 33.5 2.0 35.0 96-97 18.530460092665173 25.0 2.0 33.0 2.0 35.0 98-99 16.931877140861438 12.5 2.0 32.5 2.0 34.0 100-101 13.033346156896357 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21203348499749453 1101 2 0.26779138249919754 1101 3 0.2264929426445903 1101 4 0.10509447676766825 1101 5 0.2853576980484931 1101 6 0.28850201998307057 1101 7 0.26718720293161624 1101 8 0.2695148711500437 1101 9 0.2834771240050884 1101 10-11 0.3037767918047365 1101 12-13 0.22810615051766092 1101 14-15 0.3413096079108655 1101 16-17 0.4124414203509019 1101 18-19 0.4355299816160141 1101 20-21 0.4113248306501305 1101 22-23 0.32652029554681405 1101 24-25 0.47793544112426645 1101 26-27 0.3997159717378693 1101 28-29 0.3874249515199253 1101 30-31 0.26630937217440476 1101 32-33 0.45835896681845867 1101 34-35 0.4727626779732148 1101 36-37 0.36174003458433646 1101 38-39 0.5329171976286275 1101 40-41 0.6180992002285919 1101 42-43 0.5137232163211962 1101 44-45 0.6676374008412083 1101 46-47 0.6610776942466217 1101 48-49 0.6780406420631309 1101 50-51 0.7367898581485264 1101 52-53 0.6166909582149813 1101 54-55 0.5883680547961063 1101 56-57 0.4686719399913173 1101 58-59 0.5900943355090575 1101 60-61 0.49340005800532794 1101 62-63 0.44973160737866635 1101 64-65 0.23739476676542282 1101 66-67 0.34751721498538757 1101 68-69 0.3512517265761623 1101 70-71 0.368188766221774 1101 72-73 0.4818003482313813 1101 74-75 0.30910281577657983 1101 76-77 0.2833403425165315 1101 78-79 0.1354601271258602 1101 80-81 0.24257497586359733 1101 82-83 -0.08812925145202755 1101 84-85 -0.12251784247287745 1101 86-87 -0.0014261704450078128 1101 88-89 0.08582086266372357 1101 90-91 -0.0139342886060696 1101 92-93 -0.2936832850653275 1101 94-95 -0.4462629917489984 1101 96-97 -0.7184650391365786 1101 98-99 -0.8870258367186317 1101 100-101 -1.0754848146476697 1102 1 0.21111458114281945 1102 2 0.20747782507401524 1102 3 0.192784806258409 1102 4 0.07131282807311834 1102 5 0.198425004601404 1102 6 0.1812190349005789 1102 7 0.22281868463253574 1102 8 0.25116093242639437 1102 9 0.2631904698903753 1102 10-11 0.29068584062130043 1102 12-13 0.21525384003678028 1102 14-15 0.2279338788223768 1102 16-17 0.3159842198751619 1102 18-19 0.3647359828536523 1102 20-21 0.22299397698570544 1102 22-23 0.35262265101283674 1102 24-25 0.3326899319582779 1102 26-27 0.3318630139097962 1102 28-29 0.13855232311477295 1102 30-31 0.16562109947227555 1102 32-33 0.10471758399707909 1102 34-35 -0.024197533215577494 1102 36-37 0.282429580036883 1102 38-39 0.07310511483840543 1102 40-41 0.1039955347073942 1102 42-43 0.3179108394817405 1102 44-45 0.11429324912935357 1102 46-47 -0.05308587831990508 1102 48-49 -0.013283643770286346 1102 50-51 -0.01737510786026064 1102 52-53 -0.10803589418546977 1102 54-55 -0.20939766476870147 1102 56-57 -0.12148843753278271 1102 58-59 -0.0766623014454666 1102 60-61 -0.20302867016589943 1102 62-63 -0.08535241609261135 1102 64-65 -0.12133926350848512 1102 66-67 -0.13541778038443653 1102 68-69 -0.3849112271703312 1102 70-71 -0.55470395408474 1102 72-73 -0.4004241561639965 1102 74-75 -0.4680640966625802 1102 76-77 -0.7566550163332479 1102 78-79 -0.8035671733705136 1102 80-81 -0.7438888846078449 1102 82-83 -0.612860108627487 1102 84-85 -0.5935002302404335 1102 86-87 -0.7186451669032508 1102 88-89 -0.6598923822792848 1102 90-91 -0.8540384347002643 1102 92-93 -0.9817200894962532 1102 94-95 -1.0581690232693255 1102 96-97 -1.1365706818097046 1102 98-99 -1.5472651137963993 1102 100-101 -1.4213777807266776 1103 1 0.261655766486232 1103 2 0.24567176308022454 1103 3 0.27875601843586395 1103 4 0.2839416071479022 1103 5 0.26891493360333385 1103 6 0.2784462888317165 1103 7 0.28323909676391423 1103 8 0.22522815814859598 1103 9 0.3074886767548648 1103 10-11 0.28611068849814814 1103 12-13 0.23447305124953033 1103 14-15 0.316540051017121 1103 16-17 0.37509534213880613 1103 18-19 0.3897541623526166 1103 20-21 0.3920422320761787 1103 22-23 0.27818315724719866 1103 24-25 0.24326309021775927 1103 26-27 0.3109695287835308 1103 28-29 0.33263853602323934 1103 30-31 0.34273908298290223 1103 32-33 0.28997865867949457 1103 34-35 0.205334912550434 1103 36-37 0.23958285782712352 1103 38-39 0.3916420873127322 1103 40-41 0.4029676545964236 1103 42-43 0.5200429945500318 1103 44-45 0.5415507699876443 1103 46-47 0.4231856279881612 1103 48-49 0.24607982196278044 1103 50-51 0.4268303413018799 1103 52-53 0.47365666639586834 1103 54-55 0.4610471836065244 1103 56-57 0.3442937636181185 1103 58-59 0.25624828120987075 1103 60-61 0.2780954075066049 1103 62-63 0.21792405849434004 1103 64-65 0.13430884063943793 1103 66-67 0.06458746479091815 1103 68-69 -0.016892037147385963 1103 70-71 -0.22539494233328128 1103 72-73 -0.35119609437129995 1103 74-75 -0.43932288821251575 1103 76-77 -0.2135535284634571 1103 78-79 -0.1691544143159227 1103 80-81 -0.3148761468476202 1103 82-83 -0.4691336781540265 1103 84-85 -0.5065855835326154 1103 86-87 -0.5900945447749173 1103 88-89 -0.4373697387178801 1103 90-91 -0.48155866739785225 1103 92-93 -0.7019600362289715 1103 94-95 -0.9396013116278041 1103 96-97 -1.0872635067409107 1103 98-99 -1.0872331757319884 1103 100-101 -1.3865640184321002 1104 1 0.19170698316349544 1104 2 0.3194096249181797 1104 3 0.37955450470909113 1104 4 0.27210954162969614 1104 5 0.3812679994697916 1104 6 0.26074285101097416 1104 7 0.30362117682139456 1104 8 0.26858443086570105 1104 9 0.3485774461441906 1104 10-11 0.3221547966099436 1104 12-13 0.38096534182031405 1104 14-15 0.4006427510208468 1104 16-17 0.32019524446047853 1104 18-19 0.4136269004113231 1104 20-21 0.4929875067617999 1104 22-23 0.46675832284800833 1104 24-25 0.5692772807494535 1104 26-27 0.501930006085594 1104 28-29 0.4060714917294277 1104 30-31 0.4390833454852441 1104 32-33 0.4423026689783214 1104 34-35 0.495639620008852 1104 36-37 0.6041895116768927 1104 38-39 0.5714867904265972 1104 40-41 0.6286646081129348 1104 42-43 0.5330642268206915 1104 44-45 0.49393402106856144 1104 46-47 0.38138812014015144 1104 48-49 0.4962664681907185 1104 50-51 0.47002332422466964 1104 52-53 0.4819086709032874 1104 54-55 0.40506382356721815 1104 56-57 0.5717737694436877 1104 58-59 0.5198047411396658 1104 60-61 0.6541868589862219 1104 62-63 0.5130987260325455 1104 64-65 0.47631270606762754 1104 66-67 0.5160584603693721 1104 68-69 0.5279136479973836 1104 70-71 0.4625028456441562 1104 72-73 0.3548738199315231 1104 74-75 0.3866009962489123 1104 76-77 0.14305036563280638 1104 78-79 0.10390708789435976 1104 80-81 -0.025587218486769814 1104 82-83 -0.20463278092217152 1104 84-85 -0.11398363742038242 1104 86-87 -0.05277613591533381 1104 88-89 -0.10548179508725397 1104 90-91 -0.3682552532865593 1104 92-93 -0.3879710020129856 1104 94-95 -0.7848796751635909 1104 96-97 -1.0837253970045886 1104 98-99 -1.162833148738681 1104 100-101 -1.3228713112712054 1105 1 0.12590856319040356 1105 2 0.19656779545525893 1105 3 0.19465522644626532 1105 4 0.10961341773100486 1105 5 0.21678048407206774 1105 6 0.17105626758447556 1105 7 0.23912711828017308 1105 8 0.24266931535076708 1105 9 0.23362152701744776 1105 10-11 0.2848617235230293 1105 12-13 0.2517859089117209 1105 14-15 0.25592904199728395 1105 16-17 0.2732453450257424 1105 18-19 0.3532009702181327 1105 20-21 0.29671027092699376 1105 22-23 0.313968810084603 1105 24-25 0.2689846884413427 1105 26-27 0.3103282730060144 1105 28-29 0.3365904199056189 1105 30-31 0.11553414531341843 1105 32-33 0.13387666481916227 1105 34-35 0.2475477997916684 1105 36-37 0.2912110443086888 1105 38-39 0.21475887424711715 1105 40-41 0.15088284755216108 1105 42-43 0.3533084283153798 1105 44-45 0.3742748619478462 1105 46-47 0.32786165027986414 1105 48-49 0.25439703519677437 1105 50-51 0.1822843216842216 1105 52-53 0.25958545668361666 1105 54-55 0.22134017750008184 1105 56-57 0.08732155857239121 1105 58-59 0.13082979568957853 1105 60-61 0.009904855191201989 1105 62-63 0.10177112221703766 1105 64-65 -0.14072279475310623 1105 66-67 -0.13461164287005545 1105 68-69 -0.040973562432956356 1105 70-71 -0.04581832597680702 1105 72-73 -0.3684571806289121 1105 74-75 -0.4060866141666324 1105 76-77 -0.4406513751278496 1105 78-79 -0.5515971182089707 1105 80-81 -0.3551539770419332 1105 82-83 -0.4517730004873073 1105 84-85 -0.46309163627106287 1105 86-87 -0.4226138090597118 1105 88-89 -0.38514214904287414 1105 90-91 -0.7759699039538184 1105 92-93 -0.9424163568078292 1105 94-95 -1.0562601077507203 1105 96-97 -1.142342716989905 1105 98-99 -1.2859701308702114 1105 100-101 -1.7013814943938783 1106 1 0.18506206159362293 1106 2 0.13732927361874658 1106 3 0.11877907033495205 1106 4 0.2165386800594078 1106 5 0.19145283279257086 1106 6 0.19101603698433678 1106 7 0.29378785404393426 1106 8 0.0924982316130567 1106 9 0.37792910447171835 1106 10-11 0.31436641018057543 1106 12-13 0.36849093981047787 1106 14-15 0.3895936556590982 1106 16-17 -0.1127937035412998 1106 18-19 0.35882081178422 1106 20-21 0.4603052068483606 1106 22-23 0.5068128439280812 1106 24-25 0.2992955424084087 1106 26-27 0.23901331302923978 1106 28-29 0.19347939174419793 1106 30-31 0.18659559029703132 1106 32-33 0.2562134613020888 1106 34-35 0.19975218783032744 1106 36-37 0.13268640825045708 1106 38-39 0.2783481073772194 1106 40-41 0.21349845868400763 1106 42-43 0.10923777591605699 1106 44-45 0.3121165109877069 1106 46-47 0.37455662352859065 1106 48-49 0.3766140730633083 1106 50-51 0.2767043996569356 1106 52-53 0.36444371639910145 1106 54-55 0.1858133432750364 1106 56-57 -0.003158409077222757 1106 58-59 0.3053751276957115 1106 60-61 0.44036605547322694 1106 62-63 0.08629789422980849 1106 64-65 -0.022369657181041447 1106 66-67 -0.1973055377727988 1106 68-69 -0.22450338284687987 1106 70-71 -0.36887263728699793 1106 72-73 -0.25356771109218457 1106 74-75 -0.17770537738674363 1106 76-77 -0.6409588074201622 1106 78-79 -0.494088615865639 1106 80-81 -0.5086933366703157 1106 82-83 -0.4805487667350796 1106 84-85 -0.4972420240608706 1106 86-87 -0.7431267492115126 1106 88-89 -1.2879017815757408 1106 90-91 -1.127908334545662 1106 92-93 -1.002914158641019 1106 94-95 -1.2189524473467586 1106 96-97 -1.1554586184456745 1106 98-99 -1.008270636863326 1106 100-101 -1.1842228323723951 1107 1 -0.010592596034527446 1107 2 -0.04312747607023226 1107 3 -0.015576173781127522 1107 4 -0.019999390877707413 1107 5 -0.011939784060707836 1107 6 -0.06692440021575408 1107 7 0.08689915980471596 1107 8 0.07303559165571016 1107 9 -0.06680646817915203 1107 10-11 0.021374115768715285 1107 12-13 -3.1064484389276004E-6 1107 14-15 -0.07357978989400493 1107 16-17 -0.10825826037476816 1107 18-19 -0.023142145536724 1107 20-21 0.07581762211228238 1107 22-23 0.07088823682421719 1107 24-25 0.11401803967908819 1107 26-27 0.06869886917721146 1107 28-29 -0.014518170903379257 1107 30-31 -0.08870241412066804 1107 32-33 -0.11440089114922358 1107 34-35 -0.050767019014564596 1107 36-37 0.0639502135669332 1107 38-39 0.013742399910292136 1107 40-41 -0.012060101246930799 1107 42-43 0.010699870008018308 1107 44-45 0.04845781649459724 1107 46-47 0.04084784905985828 1107 48-49 0.024821603466740783 1107 50-51 -0.14333742886516632 1107 52-53 -0.16217769421862016 1107 54-55 -0.19784465301815146 1107 56-57 -0.2470697884663693 1107 58-59 -0.2495125890636274 1107 60-61 -0.03515124719008611 1107 62-63 0.0027208256538884257 1107 64-65 -0.15306728584024754 1107 66-67 -0.18156652490053204 1107 68-69 -0.23932775383222804 1107 70-71 -0.37656521351773264 1107 72-73 -0.19651398778246332 1107 74-75 -0.3342862289367474 1107 76-77 -0.5324512414720104 1107 78-79 -0.4363122556452623 1107 80-81 -0.3535206274007585 1107 82-83 -0.3460104038234597 1107 84-85 -0.4424511031986569 1107 86-87 -0.4821627890216007 1107 88-89 -0.6683080704527811 1107 90-91 -0.6965702661474609 1107 92-93 -0.6945348075861055 1107 94-95 -0.5956001991430355 1107 96-97 -0.9723801280808324 1107 98-99 -0.9564524608153917 1107 100-101 -1.0082190113146865 1108 1 -0.048025309439417185 1108 2 -0.0963405087776863 1108 3 0.02458981307268715 1108 4 0.013129878900180358 1108 5 0.07236307987429313 1108 6 0.030187476750000997 1108 7 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-0.6494380775305046 2316 56-57 -0.5705441830512186 2316 58-59 -0.40373571759934634 2316 60-61 -0.5379809804905662 2316 62-63 -0.276843714593781 2316 64-65 -0.21203399889320096 2316 66-67 -0.3635642142334774 2316 68-69 -0.39265010207773443 2316 70-71 -0.42934644504583375 2316 72-73 -0.19395733752702782 2316 74-75 -0.1645390589016671 2316 76-77 -0.3238403470567057 2316 78-79 -0.18595324061573493 2316 80-81 -0.31685847583063875 2316 82-83 -0.4524569703426913 2316 84-85 -0.3336292469195534 2316 86-87 -0.3637584741705169 2316 88-89 -0.36600537121565324 2316 90-91 -0.08951036298423887 2316 92-93 -0.20064732799205487 2316 94-95 -0.40313826400106834 2316 96-97 -0.314969872573311 2316 98-99 -0.3408859860557705 2316 100-101 -0.1852502488649712 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7907.0 3 2550.0 4 2363.0 5 2682.0 6 3284.0 7 4131.0 8 5122.0 9 6562.0 10 8163.0 11 10120.0 12 12383.0 13 15268.0 14 17814.0 15 21387.0 16 24853.0 17 31109.0 18 31393.0 19 27783.0 20 29149.0 21 31742.0 22 34857.0 23 38740.0 24 43305.0 25 48728.0 26 55621.0 27 62749.0 28 72030.0 29 83775.0 30 96864.0 31 114990.0 32 138583.0 33 170704.0 34 215181.0 35 277128.0 36 342768.0 37 353306.0 38 168458.0 39 9223.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.89648486496125 17.97422593222041 13.574321999825123 23.554967202993215 2 15.73697048211753 19.292979231660713 40.39190598589911 24.578144300322652 3 19.688552892508014 23.456674147537747 29.10650751772172 27.748265442232515 4 12.89568134520872 15.751737766153006 35.96296888761968 35.38961200101859 5 14.02313701280245 37.02743403941896 33.840268885065235 15.10916006271336 6 33.541121075804895 35.03135535753512 16.84593564654308 14.5815879201169 7 29.71024539026757 32.165981303271295 19.749159670765863 18.37461363569527 8 26.909030139127143 35.249027819323956 18.94390197000128 18.898040071547616 9 26.68145557353587 16.589229140636245 19.68512684140787 37.044188444420016 10-11 25.692594507335148 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22.284205269687003 36-37 22.69503911477107 26.179677797093948 28.44140626565225 22.683876822482727 38-39 22.41643714379449 26.389134730561764 28.542294516628647 22.6521336090151 40-41 22.615683748317302 26.283136261331073 28.591174422217904 22.510005568133717 42-43 22.546990422980574 26.254031756544855 28.419143203566794 22.779834616907777 44-45 22.419204789525658 26.502629379708974 28.46569104797955 22.61247478278581 46-47 22.564050429329853 26.705388815412768 27.955326349053998 22.775234406203378 48-49 22.68967375787512 26.687058587483765 27.85377703498191 22.76949061965921 50-51 22.64862016103605 26.920311894095327 27.700428616906976 22.73063932796165 52-53 22.930776919970146 26.72024161164947 27.716157416747677 22.632824051632706 54-55 22.49430407693621 26.707656331510826 27.894582745620887 22.903456845932077 56-57 22.577113722026386 27.049860243952388 27.702150477352045 22.67087555666918 58-59 22.7020484178426 26.61716746350885 27.760835953763447 22.919948164885103 60-61 22.636641574557054 26.649224055546306 28.03811056052268 22.676023809373962 62-63 22.601061735704413 26.69592746993173 28.024546725221334 22.678464069142525 64-65 22.329994879320754 26.68684424076156 27.97784662564402 23.00531425427367 66-67 22.35742455730681 26.83429526966862 27.78865384919796 23.01962632382662 68-69 22.539286411945326 26.652837381648588 27.86887716481598 22.938999041590105 70-71 22.412616737842868 26.810167353250463 27.699435950286716 23.077779958619953 72-73 22.596875907207263 26.76181796610855 27.62859788148864 23.012708245195547 74-75 22.487977683712472 26.940630908882152 27.557109578449356 23.014281828956015 76-77 22.5987837656434 26.77456494413834 27.51886589540354 23.10778539481472 78-79 22.702525833427405 26.91557876945325 27.4403512491877 22.941544147931648 80-81 22.65881144219618 27.136888142757826 27.282514637970284 22.921785777075716 82-83 22.728872702311037 27.074574784532075 27.254423766303105 22.942128746853786 84-85 22.7440634553448 26.91785900943597 27.18560581576114 23.15247171945809 86-87 22.85692602023061 26.897690052173523 27.145753114231617 23.099630813364247 88-89 22.804016480726258 26.889478485509926 27.11385840753208 23.192646626231735 90-91 22.831386708211973 26.961583378518245 27.147399763454366 23.059630149815412 92-93 23.06676822206441 26.635453391300302 27.14303322212356 23.15474516451173 94-95 22.921343769979146 26.544457657615716 27.119284664812078 23.414913907593064 96-97 23.155381331713528 26.357399421724036 27.01436990584129 23.472849340721144 98-99 23.04540384063677 26.381068138951452 26.763407435892738 23.810120584519034 100-101 23.89792472157244 25.298471363891295 26.930056279189984 23.873547635346277 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1222.0 1 850.0 2 714.0 3 952.0 4 778.5 5 691.5 6 1265.0 7 2564.5 8 4144.0 9 4886.5 10 4048.0 11 2991.0 12 2782.5 13 2884.0 14 3052.5 15 3260.0 16 3420.0 17 3547.0 18 3722.0 19 3964.5 20 4455.0 21 5158.0 22 6126.0 23 7352.0 24 8946.0 25 11145.5 26 13780.5 27 16874.0 28 19931.5 29 22913.0 30 26386.0 31 30772.0 32 36069.0 33 39590.5 34 42834.0 35 47041.0 36 51647.0 37 56953.0 38 61383.0 39 64173.5 40 67543.5 41 73134.5 42 78105.5 43 83491.0 44 89379.0 45 94513.5 46 96952.5 47 98819.0 48 103428.0 49 105385.5 50 104395.0 51 102101.0 52 98610.0 53 96305.0 54 94444.5 55 89364.5 56 81877.0 57 74410.0 58 67462.5 59 59665.5 60 50107.5 61 40942.0 62 33236.5 63 26269.0 64 20450.0 65 16245.0 66 12549.5 67 9856.0 68 7570.5 69 5423.5 70 4027.5 71 3000.5 72 2313.0 73 1851.5 74 1469.0 75 1179.5 76 821.0 77 519.0 78 326.5 79 202.0 80 122.0 81 71.0 82 39.0 83 29.5 84 24.0 85 15.0 86 12.5 87 11.0 88 10.0 89 6.5 90 9.0 91 8.5 92 3.5 93 3.0 94 3.0 95 2.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1443889985267518 2 0.05269226193925826 3 0.15620853629894432 4 0.13211193026015183 5 0.1964330922655995 6 0.1983013417844299 7 0.1699344511311679 8 0.07106973679795758 9 0.12372387119601522 10-11 0.1050223122371106 12-13 0.15571287826333624 14-15 0.14114815752469903 16-17 0.13834578324645339 18-19 0.1359818756920149 20-21 0.1376785512754425 22-23 0.20156124655708302 24-25 0.21967182862737802 26-27 0.15182386906087292 28-29 0.12991959664111613 30-31 0.10143832336425222 32-33 0.12812760220468694 34-35 0.09629110530216839 36-37 0.08946627542725723 38-39 0.05322604751606695 40-41 0.06140440510360016 42-43 0.06519809545306195 44-45 0.04022455596665518 46-47 0.05204409373884769 48-49 0.04382760861011386 50-51 0.05480834047589272 52-53 0.02733744704084527 54-55 0.05274945325105918 56-57 0.02695617162883906 58-59 0.052882899645261355 60-61 0.03948106891324307 62-63 0.053683578010474395 64-65 0.040167364654854244 66-67 0.040701150231662944 68-69 0.06740949284269797 70-71 0.05583778408830949 72-73 0.053645450469273784 74-75 0.045486156652340876 76-77 0.022266484061162675 78-79 0.022571504390767643 80-81 0.02504979456880801 82-83 0.021961463731557707 84-85 0.019921640277324484 86-87 0.02518324096301018 88-89 0.06767638563110231 90-91 0.11382977425445405 92-93 0.08399497326496812 94-95 0.07909558422068831 96-97 0.07175603253956876 98-99 0.05886892361375885 100-101 0.13394205223778166 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2622776.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.1596913877066 #Duplication Level Percentage of deduplicated Percentage of total 1 75.90790921313173 40.352410276561336 2 12.962332492604006 13.781471899433411 3 4.502418278929862 7.180414986188417 4 2.072641286165126 4.407238845198296 5 1.1763367515291079 3.126684933965234 6 0.7359109468648835 2.3472479294503295 7 0.4855942068716608 1.8069826723868951 8 0.3503616251324836 1.4900092692910543 9 0.25735201992133444 1.2312678561318955 >10 1.40527696734796 13.441790468403141 >50 0.09279347356545745 3.4182005546018566 >100 0.04632846889941315 4.428848949651347 >500 0.0033784946172590257 1.1733594354209471 >1k 0.0011501258271415554 0.8276490591936022 >5k 1.4376572839269443E-4 0.5637903051545504 >10k+ 7.188286419634722E-5 0.422632558967692 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11060 0.4216906056788685 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9187 0.35027772101010535 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5567 0.21225602186385725 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.812754120062102E-5 0.0 14-15 0.0 0.0 0.0 1.3344639420217357E-4 0.0 16-17 0.0 0.0 0.0 1.7157393540279462E-4 0.0 18-19 0.0 0.0 0.0 2.2876524720372613E-4 0.0 20-21 0.0 0.0 0.0 3.240841002052787E-4 0.0 22-23 0.0 0.0 0.0 5.528493474090048E-4 0.0 24-25 0.0 0.0 0.0 7.05359512211489E-4 0.0 26-27 0.0 0.0 0.0 0.0013154001714214252 0.0 28-29 0.0 0.0 0.0 0.003850881661262723 0.0 30-31 0.0 0.0 0.0 0.012086430560596863 0.0 32-33 0.0 0.0 0.0 0.030730798207700544 0.0 34-35 0.0 0.0 0.0 0.061842871827407296 0.0 36-37 0.0 1.906377060031051E-5 0.0 0.1120568435886252 0.0 38-39 0.0 3.812754120062102E-5 0.0 0.1854332966292203 0.0 40-41 0.0 3.812754120062102E-5 0.0 0.26471951855591175 0.0 42-43 0.0 3.812754120062102E-5 0.0 0.34164183292816463 0.0 44-45 0.0 3.812754120062102E-5 0.0 0.43669379314131285 0.0 46-47 0.0 3.812754120062102E-5 0.0 0.5462723465518977 0.0 48-49 0.0 3.812754120062102E-5 0.0 0.6634573444320064 0.0 50-51 0.0 3.812754120062102E-5 0.0 0.7858848792272005 0.0 52-53 0.0 3.812754120062102E-5 0.0 0.9025742190717012 0.0 54-55 0.0 3.812754120062102E-5 0.0 1.0285666789691532 0.0 56-57 0.0 3.812754120062102E-5 0.0 1.1761774547273576 0.0 58-59 0.0 3.812754120062102E-5 0.0 1.3345020695629364 0.0 60-61 0.0 3.812754120062102E-5 0.0 1.499994662144232 0.0 62-63 0.0 3.812754120062102E-5 0.0 1.6586624248506163 0.0 64-65 0.0 3.812754120062102E-5 0.0 1.8227442984074889 0.0 66-67 0.0 3.812754120062102E-5 0.0 2.0062902817472787 0.0 68-69 0.0 3.812754120062102E-5 0.0 2.214466656702669 0.0 70-71 0.0 3.812754120062102E-5 0.0 2.4311264095751985 0.0 72-73 0.0 3.812754120062102E-5 0.0 2.6466995275235092 0.0 74-75 0.0 3.812754120062102E-5 0.0 2.861452903336007 3.812754120062102E-5 76-77 0.0 3.812754120062102E-5 0.0 3.094984093189811 3.812754120062102E-5 78-79 0.0 3.812754120062102E-5 0.0 3.3451198272364855 3.812754120062102E-5 80-81 0.0 3.812754120062102E-5 0.0 3.614891245001479 3.812754120062102E-5 82-83 0.0 3.812754120062102E-5 0.0 3.8927075739598047 3.812754120062102E-5 84-85 0.0 3.812754120062102E-5 0.0 4.160686997288369 3.812754120062102E-5 86-87 0.0 3.812754120062102E-5 0.0 4.441038807736536 3.812754120062102E-5 88-89 0.0 3.812754120062102E-5 0.0 4.73595533892334 3.812754120062102E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5065 0.0 53.964108 1 GTATCAA 9165 0.0 40.578045 1 ATCAACG 11035 0.0 33.275715 3 TATCAAC 11275 0.0 32.734898 2 TCAACGC 11325 0.0 32.38162 4 CAACGCA 11555 0.0 31.73707 5 AACGCAG 11850 0.0 31.100414 6 GTGGTAT 2415 0.0 28.196257 1 GTACATG 10180 0.0 27.317291 1 ACGCAGA 13470 0.0 26.963469 7 CGCAGAG 13670 0.0 26.462627 8 TGGTATC 2425 0.0 26.085892 2 TACATGG 10540 0.0 25.6766 2 ACATGGG 10535 0.0 24.154701 3 GCAGAGT 14930 0.0 23.71947 9 GAGTACT 9045 0.0 22.439829 12-13 TACACCG 305 9.094947E-12 21.832859 5 GTACTTT 9345 0.0 21.691322 14-15 CAGAGTA 14410 0.0 21.275276 10-11 CATGGGG 5690 0.0 21.232635 4 >>END_MODULE