FastQCFastQC Report
Thu 26 May 2016
SRR938012_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938012_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2622776
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110860.4226819217500846No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92470.3525653734821426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58910.22460934521285844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47500.055.912941
GTATCAA88250.040.9698221
ATCAACG110450.032.2938083
TCAACGC112350.031.7029844
TATCAAC114650.031.3232882
CAACGCA114350.031.1900235
AACGCAG117400.030.379146
GTGGTAT23200.030.1039491
TGGTATC23100.027.7614022
GTACATG106050.027.6419471
TACATGG108750.026.1211012
ACGCAGA137450.025.8094967
ACATGGG106350.025.4555453
CGCAGAG139150.025.3235388
GCAGAGT151400.022.929539
CATGGGG56400.021.8088424
GAGTACT93050.021.33560612-13
GTACTTT95750.020.65917814-15
AGTACTT96050.020.19946112-13
CAGAGTA148650.020.14452210-11
Produced by FastQC (version 0.11.5)