##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938011_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 445211 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.014642495356135 33.0 31.0 34.0 31.0 34.0 2 32.02695126580431 33.0 31.0 34.0 30.0 34.0 3 32.0379348219159 34.0 31.0 34.0 30.0 34.0 4 35.58022825132353 37.0 35.0 37.0 33.0 37.0 5 35.66263636792442 37.0 35.0 37.0 35.0 37.0 6 35.81359400374205 37.0 36.0 37.0 35.0 37.0 7 35.80440510229981 37.0 36.0 37.0 35.0 37.0 8 35.82517727549409 37.0 36.0 37.0 35.0 37.0 9 37.36390834907493 39.0 37.0 39.0 35.0 39.0 10-11 37.314383517029 39.0 37.0 39.0 34.5 39.0 12-13 37.17779659532222 39.0 37.0 39.0 34.5 39.0 14-15 37.974444701501085 40.0 38.0 41.0 33.0 41.0 16-17 37.89923878790057 40.0 37.5 41.0 33.5 41.0 18-19 38.38764091632956 40.0 38.0 41.0 34.5 41.0 20-21 38.442495805359705 40.0 38.0 41.0 34.5 41.0 22-23 38.336293353039345 40.0 38.0 41.0 34.5 41.0 24-25 38.148329668404415 40.0 37.5 41.0 34.0 41.0 26-27 37.68536154767065 40.0 36.5 41.0 33.5 41.0 28-29 37.36214738629549 39.5 35.5 41.0 33.0 41.0 30-31 36.95722589962961 38.5 35.0 40.5 33.0 41.0 32-33 36.42887192814194 38.0 35.0 40.0 33.0 41.0 34-35 36.08491030095842 38.0 35.0 40.0 32.0 41.0 36-37 35.625599996406194 37.0 35.0 40.0 31.0 41.0 38-39 35.06500064014591 35.5 35.0 40.0 30.0 41.0 40-41 34.661162909272235 35.5 35.0 40.0 28.5 41.0 42-43 34.442299269335216 35.0 35.0 40.0 26.5 41.0 44-45 33.93775535644896 35.0 34.5 40.0 23.5 41.0 46-47 33.158868491569166 35.0 33.0 40.0 19.5 41.0 48-49 32.52357533843504 35.0 33.0 40.0 11.5 41.0 50-51 31.59929561488822 35.0 32.0 38.5 4.0 40.5 52-53 31.41521435903426 35.0 31.5 38.5 2.0 40.0 54-55 31.50429571596389 35.0 32.5 39.0 2.0 40.0 56-57 31.279607871323932 35.0 32.0 39.0 2.0 40.5 58-59 30.923884405371837 35.0 31.0 38.5 2.0 40.5 60-61 30.381686436318958 35.0 30.5 38.0 2.0 40.0 62-63 29.75809335348857 35.0 29.5 37.5 2.0 40.0 64-65 28.9923440795488 35.0 28.0 36.5 2.0 39.5 66-67 28.395574233341044 35.0 26.0 36.0 2.0 39.0 68-69 27.907004768525486 34.5 26.0 35.5 2.0 39.0 70-71 27.171274968498082 34.0 25.0 35.0 2.0 37.5 72-73 26.33282758063031 33.5 21.0 35.0 2.0 37.0 74-75 25.630953637713354 33.0 18.0 35.0 2.0 36.5 76-77 24.753007001174723 32.5 8.0 35.0 2.0 35.5 78-79 24.046378009528066 32.0 2.0 35.0 2.0 35.0 80-81 23.504828047824514 31.5 2.0 35.0 2.0 35.0 82-83 22.832979194134914 31.0 2.0 35.0 2.0 35.0 84-85 22.15839455898439 31.0 2.0 35.0 2.0 35.0 86-87 21.371108305949313 30.0 2.0 35.0 2.0 35.0 88-89 20.504500113429362 29.0 2.0 34.0 2.0 35.0 90-91 19.67387149014737 27.0 2.0 34.0 2.0 35.0 92-93 19.000364995474058 25.5 2.0 34.0 2.0 35.0 94-95 18.264062433318134 24.5 2.0 34.0 2.0 35.0 96-97 17.217033047251753 17.5 2.0 33.0 2.0 35.0 98-99 16.130493181884546 2.0 2.0 33.0 2.0 35.0 100-101 13.495806482768845 2.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24961737629970315 1101 2 0.27255081643055945 1101 3 0.15552082099667786 1101 4 0.1532853491281827 1101 5 0.09923538851879954 1101 6 0.16552261090946985 1101 7 0.22531144141839832 1101 8 0.05823725710459371 1101 9 0.20284281135436544 1101 10-11 0.15408551327868736 1101 12-13 0.354852583580616 1101 14-15 0.18505648314467038 1101 16-17 0.43514728902680844 1101 18-19 0.4068018545008343 1101 20-21 0.4628709574091303 1101 22-23 0.30141543991467046 1101 24-25 0.45561613067993534 1101 26-27 0.49388858781043865 1101 28-29 0.5739831314191761 1101 30-31 0.6869819579021481 1101 32-33 0.9192165576546572 1101 34-35 0.787814568606187 1101 36-37 0.9708658497121618 1101 38-39 0.9657507099068567 1101 40-41 0.9851306074583448 1101 42-43 1.0379586922906583 1101 44-45 1.4088585706812182 1101 46-47 1.3098164369679068 1101 48-49 1.9755310946959384 1101 50-51 2.0699911277071585 1101 52-53 2.089631025715189 1101 54-55 2.065342233115789 1101 56-57 2.3585143143775333 1101 58-59 2.7348192068602266 1101 60-61 2.7019306049729472 1101 62-63 2.4594610158955312 1101 64-65 2.14636843934883 1101 66-67 2.580848060661701 1101 68-69 2.7720578486541925 1101 70-71 3.3189765517771157 1101 72-73 3.472708751065902 1101 74-75 3.606823681401412 1101 76-77 3.4208705294306014 1101 78-79 3.7527290912629283 1101 80-81 3.897881696741006 1101 82-83 4.280873309266116 1101 84-85 4.530079493745443 1101 86-87 4.501745050333557 1101 88-89 4.471985026084589 1101 90-91 4.537176463283714 1101 92-93 4.775136893603438 1101 94-95 5.051810760339439 1101 96-97 4.579239688693086 1101 98-99 4.617131270091054 1101 100-101 3.864188034686004 1102 1 0.3323654513751464 1102 2 0.4235609789496735 1102 3 0.4301659447466619 1102 4 0.3029220153888943 1102 5 0.29191052620672764 1102 6 0.3709178124683561 1102 7 0.4518774301327255 1102 8 0.4944204260243339 1102 9 0.38897523533844947 1102 10-11 0.5194591989848547 1102 12-13 0.6249408121087399 1102 14-15 0.8109208385486752 1102 16-17 0.7804902571319232 1102 18-19 0.7828319766946379 1102 20-21 0.7611260082217228 1102 22-23 0.8102044627701872 1102 24-25 0.7187455370318574 1102 26-27 0.7350657349978178 1102 28-29 0.8614836388685347 1102 30-31 1.0017933897212572 1102 32-33 1.1305117037324095 1102 34-35 1.026099254461208 1102 36-37 1.2346143442790734 1102 38-39 1.1389194943288388 1102 40-41 1.3787861373031376 1102 42-43 1.6221377745515753 1102 44-45 1.6228905602886528 1102 46-47 1.5239431504552314 1102 48-49 1.8258267685210186 1102 50-51 1.652952331512349 1102 52-53 1.5657301610214809 1102 54-55 1.551018629278115 1102 56-57 1.8529289884597269 1102 58-59 1.8156626553507387 1102 60-61 2.034949319705209 1102 62-63 2.1412429073960944 1102 64-65 1.9438081881952236 1102 66-67 2.281348067427693 1102 68-69 2.3469912036386944 1102 70-71 2.2650008552182967 1102 72-73 2.1596091150762433 1102 74-75 2.5347895537406835 1102 76-77 2.66654029492134 1102 78-79 2.8243606424341188 1102 80-81 2.4714178744971314 1102 82-83 2.621869127883418 1102 84-85 2.387658757334936 1102 86-87 2.3580658395187974 1102 88-89 2.441947407172293 1102 90-91 2.617133566898108 1102 92-93 2.6311802771404267 1102 94-95 2.1043486837213514 1102 96-97 2.0102468741759765 1102 98-99 1.8672970368808635 1102 100-101 1.3342722036192765 1103 1 0.24538457206690367 1103 2 0.34688944076918204 1103 3 0.26398642946228534 1103 4 -0.045921000078166685 1103 5 0.14394438322779735 1103 6 0.010760706147564747 1103 7 0.022136838243795864 1103 8 -0.07271512384778589 1103 9 0.0020491605607162455 1103 10-11 0.24972043391360899 1103 12-13 0.20657216030019043 1103 14-15 0.6507972238854123 1103 16-17 0.5650414689209882 1103 18-19 0.5202939179928947 1103 20-21 0.5190878886260677 1103 22-23 0.6831614716606964 1103 24-25 0.7027060777698821 1103 26-27 0.7397880587099124 1103 28-29 0.6343005917366327 1103 30-31 0.6782517991719885 1103 32-33 0.5983170867551806 1103 34-35 0.9158568966485134 1103 36-37 0.9885907174370345 1103 38-39 1.000142244298388 1103 40-41 1.1143634116911443 1103 42-43 0.9325354118673772 1103 44-45 0.8477474595701011 1103 46-47 0.6710598232112943 1103 48-49 0.9038379730028154 1103 50-51 0.9979011806172089 1103 52-53 1.4205834066675713 1103 54-55 1.5878290056025612 1103 56-57 1.8917153725785916 1103 58-59 2.085480582521601 1103 60-61 1.9527242557665865 1103 62-63 1.810783767218286 1103 64-65 1.5663419843223743 1103 66-67 1.5422237220373631 1103 68-69 1.8170313936277367 1103 70-71 1.9505903083908684 1103 72-73 2.267814571171723 1103 74-75 2.2184638930416227 1103 76-77 2.5463996299597014 1103 78-79 2.8011417896756257 1103 80-81 2.8164002152595238 1103 82-83 3.1757145791073818 1103 84-85 3.4173810810470293 1103 86-87 3.8386498122383195 1103 88-89 3.867355396454961 1103 90-91 4.295245246352497 1103 92-93 4.2408776343441765 1103 94-95 3.9897737233024024 1103 96-97 3.9042396886930852 1103 98-99 4.049406402366188 1103 100-101 3.5059869764849463 1104 1 0.5295805693063755 1104 2 0.3789460315214299 1104 3 0.37475247501093634 1104 4 0.548730483703757 1104 5 0.4911148456156482 1104 6 0.35599880138565965 1104 7 0.4230340087061819 1104 8 0.39226072156284175 1104 9 0.4830360480693372 1104 10-11 0.4330710205250696 1104 12-13 0.5708061147515409 1104 14-15 0.5948793494892755 1104 16-17 0.8315701093626799 1104 18-19 0.6942414680274069 1104 20-21 0.5198642861415905 1104 22-23 0.5291587111362048 1104 24-25 0.35422436624470066 1104 26-27 0.5108543112979049 1104 28-29 0.503072158334291 1104 30-31 0.5401565610767562 1104 32-33 0.6111880322348213 1104 34-35 0.7038486150750103 1104 36-37 0.5235458589139128 1104 38-39 0.694759221524059 1104 40-41 0.7357057167291003 1104 42-43 0.7559826168363202 1104 44-45 0.8733168177481545 1104 46-47 1.0238721075453157 1104 48-49 1.1624991186204738 1104 50-51 0.9429494897959536 1104 52-53 1.101535787619948 1104 54-55 1.0954204479766112 1104 56-57 1.2219258625716911 1104 58-59 1.1113432464277473 1104 60-61 1.1075551736409857 1104 62-63 1.4858010204964067 1104 64-65 1.4589403280076745 1104 66-67 1.386633659925561 1104 68-69 1.6860790126753535 1104 70-71 1.7286268853404927 1104 72-73 2.1961962136837982 1104 74-75 2.1445508495633625 1104 76-77 2.3800952821336168 1104 78-79 2.332956834534148 1104 80-81 2.650872264948969 1104 82-83 2.8041652347319506 1104 84-85 2.710548782910383 1104 86-87 3.1368382180354217 1104 88-89 2.9489806552541324 1104 90-91 3.302129304323511 1104 92-93 3.5545249773393444 1104 94-95 3.368983523164374 1104 96-97 2.856154802564724 1104 98-99 2.5829122684807473 1104 100-101 2.170376210439395 1105 1 0.31123685122084055 1105 2 0.40774794093884736 1105 3 0.37013957955842614 1105 4 0.16421925554902828 1105 5 0.13019593848172661 1105 6 0.039688429255811286 1105 7 0.1603112529027726 1105 8 0.07514710893131138 1105 9 0.25061267147577126 1105 10-11 0.11060438369305103 1105 12-13 0.2923627948437968 1105 14-15 0.6041424337033092 1105 16-17 0.5175977408359316 1105 18-19 0.43378786664123936 1105 20-21 0.37426547034758784 1105 22-23 0.38852851158831214 1105 24-25 0.2683321381702939 1105 26-27 0.33491021683681765 1105 28-29 0.39076479261888863 1105 30-31 0.5734107178463503 1105 32-33 0.5411798850811564 1105 34-35 0.7744362428763125 1105 36-37 0.802932986411129 1105 38-39 0.6269944103429523 1105 40-41 0.9426548945550763 1105 42-43 0.9529601375771364 1105 44-45 0.7566604784593665 1105 46-47 0.5550407077266186 1105 48-49 0.8707478248295288 1105 50-51 1.1306208955815755 1105 52-53 0.8450037211116346 1105 54-55 1.0566956496473487 1105 56-57 1.315227984274106 1105 58-59 1.4523819511450675 1105 60-61 1.3509427830825054 1105 62-63 1.4946883029042972 1105 64-65 1.1848947498466345 1105 66-67 1.5126965192322501 1105 68-69 1.574242116396654 1105 70-71 1.5477371243619125 1105 72-73 1.746298339976157 1105 74-75 1.7450276301754322 1105 76-77 1.7523972546627888 1105 78-79 1.4121190546908942 1105 80-81 1.6958652079074135 1105 82-83 2.057076981550548 1105 84-85 1.7380970634019661 1105 86-87 1.8739031772422798 1105 88-89 1.8322038298900907 1105 90-91 1.5781629027262127 1105 92-93 1.308237095943543 1105 94-95 1.4252476515910537 1105 96-97 1.2533715742263993 1105 98-99 1.3948650608889785 1105 100-101 1.0061622960189336 1106 1 0.6872035745494074 1106 2 0.4683180121977557 1106 3 0.5682066957678558 1106 4 0.6316574246566589 1106 5 0.5027685764121799 1106 6 0.414450618131319 1106 7 0.34996351393777303 1106 8 0.29102895198159473 1106 9 0.6046332273627897 1106 10-11 0.5124320114356138 1106 12-13 0.6289316728255798 1106 14-15 0.6619741591144859 1106 16-17 0.7310423716517533 1106 18-19 0.7197297112675543 1106 20-21 0.7265749118713742 1106 22-23 0.604770589241383 1106 24-25 0.653552387857907 1106 26-27 0.8513825069157335 1106 28-29 0.8403263195633173 1106 30-31 0.8873129591810169 1106 32-33 1.003270821803703 1106 34-35 0.9938754026291079 1106 36-37 1.2508565716460112 1106 38-39 1.0233875613825703 1106 40-41 1.310859687926758 1106 42-43 1.5277904333072314 1106 44-45 1.3224032934676444 1106 46-47 1.433647275253719 1106 48-49 1.8081936489224688 1106 50-51 1.8574983370153184 1106 52-53 1.9344741762455406 1106 54-55 1.9214444422985615 1106 56-57 1.7936054651084952 1106 58-59 1.9348430289219642 1106 60-61 2.071918568562797 1106 62-63 1.7973894995586157 1106 64-65 2.061179492785474 1106 66-67 2.3032144690470666 1106 68-69 2.152508712517374 1106 70-71 2.1420538606364126 1106 72-73 2.4393390577436023 1106 74-75 2.573598174242253 1106 76-77 2.1979399143198926 1106 78-79 2.78560353645965 1106 80-81 3.077086290637542 1106 82-83 2.8145940645508496 1106 84-85 2.6390466193002453 1106 86-87 2.446904156630108 1106 88-89 2.652669095394252 1106 90-91 2.6298818972213773 1106 92-93 3.285229925023483 1106 94-95 2.9534444522574894 1106 96-97 2.5925549674112105 1106 98-99 1.78371017126711 1106 100-101 1.0987538462660336 1107 1 0.4619825574148848 1107 2 0.3601449352746755 1107 3 0.15398185070770865 1107 4 0.17312661896945514 1107 5 0.2596952989787127 1107 6 0.23019751933438215 1107 7 0.19116156351851998 1107 8 0.1844964146137542 1107 9 0.337191101952655 1107 10-11 0.3020555987487725 1107 12-13 0.33765045700349106 1107 14-15 0.27595748029566636 1107 16-17 0.4614242528037735 1107 18-19 0.27792899125297055 1107 20-21 0.2964459472341261 1107 22-23 0.3398006657998991 1107 24-25 0.4154121217259288 1107 26-27 0.4100147070615634 1107 28-29 0.5167868554729012 1107 30-31 0.6627527149229095 1107 32-33 0.4498051819932769 1107 34-35 0.2659828123994288 1107 36-37 0.5526474939938026 1107 38-39 0.6825483798295195 1107 40-41 0.6051257743285134 1107 42-43 0.37269108952305174 1107 44-45 0.28858995041259305 1107 46-47 0.1827814349328989 1107 48-49 0.13875097666581837 1107 50-51 -0.027339203998174355 1107 52-53 0.11115117223533133 1107 54-55 0.27996499461354674 1107 56-57 0.5210537425419624 1107 58-59 0.79855292684395 1107 60-61 0.6880607942281323 1107 62-63 0.6312279064124233 1107 64-65 0.933787039267429 1107 66-67 0.6425213410849828 1107 68-69 0.6880822178035615 1107 70-71 0.7965152168157772 1107 72-73 1.0207793147614659 1107 74-75 1.3128549186826497 1107 76-77 1.6463996299597028 1107 78-79 1.7869934380272738 1107 80-81 2.018277504636817 1107 82-83 2.278564853832659 1107 84-85 2.3378481140140615 1107 86-87 2.563729940443448 1107 88-89 2.7392189495685138 1107 90-91 2.6583862719935247 1107 92-93 2.4381532753698174 1107 94-95 2.576701378980058 1107 96-97 2.576263498216896 1107 98-99 2.3829343327691177 1107 100-101 2.413931115679084 1108 1 0.20125151214436698 1108 2 0.26018404090589087 1108 3 -0.012044557095016728 1108 4 -3.183571070977109E-4 1108 5 0.05441373843399333 1108 6 -0.11688796097478615 1108 7 0.06916349579676506 1108 8 0.12226095270723647 1108 9 0.23341165771268635 1108 10-11 0.0921503723961763 1108 12-13 0.09237188688939568 1108 14-15 0.3225276864053441 1108 16-17 0.5780740503746244 1108 18-19 0.21966735157685235 1108 20-21 0.24143352544538033 1108 22-23 0.1546470036725509 1108 24-25 0.02505741064754119 1108 26-27 0.4046376827700584 1108 28-29 0.3413750961795614 1108 30-31 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3.73266801005002 2316 90-91 3.7480442285407953 2316 92-93 3.951310204318734 2316 94-95 3.470289899238427 2316 96-97 3.9088016661558243 2316 98-99 3.8023871366446294 2316 100-101 3.7238804697601164 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2120.0 3 374.0 4 279.0 5 323.0 6 303.0 7 428.0 8 538.0 9 694.0 10 996.0 11 1428.0 12 2442.0 13 3516.0 14 4168.0 15 5709.0 16 7536.0 17 10315.0 18 9455.0 19 6313.0 20 6296.0 21 6960.0 22 7698.0 23 8882.0 24 10099.0 25 11004.0 26 11606.0 27 12847.0 28 14235.0 29 15399.0 30 17066.0 31 18801.0 32 21937.0 33 26886.0 34 38337.0 35 73262.0 36 43569.0 37 30448.0 38 12273.0 39 669.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 56.649604257334204 16.809705278823824 13.671950752023813 12.868739711818161 2 14.408795889620032 25.727842342575098 39.96162806632863 19.90173370147624 3 24.325678590688476 35.620843070285815 29.672613697645154 10.38086464138055 4 10.625567329037361 18.165763433902235 37.47921665902739 33.72945257803302 5 10.071328086726323 44.732193821828105 29.598908729712846 15.59756936173273 6 20.705796166750186 38.79609259305035 18.836811602670554 21.661299637528906 7 21.672351114646105 45.36099614895501 17.07738488431137 15.889267852087519 8 23.389861734586127 29.712909415259077 19.529034838211043 27.368194011943757 9 29.22295020086818 22.299064412629082 20.29015790845444 28.1878274780483 10-11 22.619571859946795 28.91607951685959 22.772687648285284 25.69166097490833 12-13 34.250604093343235 28.336162358721918 22.921845880089016 14.491387667845835 14-15 28.755040832144502 27.13489772306033 31.515788054772365 12.594273390022803 16-17 15.327056820007257 32.51909408816251 32.94652756637014 19.207321525460095 18-19 14.001368211877383 32.928860845662776 38.563242729548996 14.506528212910844 20-21 17.877905833742442 29.34890675605994 35.33857814815273 17.43460926204489 22-23 16.018071693670862 29.403009552566072 33.781980427573934 20.796938326189128 24-25 17.269032989428958 29.762502626189086 37.215037418279174 15.75342696610278 26-27 20.25056929694776 26.130364788183343 38.97664731000873 14.642418604860172 28-29 15.59881194703189 27.565502428678627 41.98917991094096 14.84650571334852 30-31 14.434855484056513 34.58743753819607 38.219546500341515 12.758160477405903 32-33 13.766452024570752 33.59998831675961 35.94337672608643 16.69018293258321 34-35 15.471607395815898 29.683917504232294 36.416496008106236 18.42797909184557 36-37 18.415870280520398 29.991001688447223 38.116443319062604 13.476684711969778 38-39 21.057946222077952 26.480555234187474 39.308720407147774 13.152778136586802 40-41 14.857920753712458 29.85860029001037 42.07149620414394 13.211982752133238 42-43 13.085895599921377 34.26647572515654 38.133602897818214 14.514025777103868 44-45 13.88161323365561 35.37215163713475 34.38645634906373 16.359778780145923 46-47 13.810006267676982 32.38400866242382 31.8535025980532 21.952482471846 48-49 16.354936538245536 31.65831742109401 33.542738402785574 18.444007637874872 50-51 20.15928764212283 33.023039538872276 33.258567803948836 13.559105015056064 52-53 14.75472066828917 32.9169254475271 37.205256555251445 15.12309732893228 54-55 12.03886831740213 32.34590196891029 37.94568902875254 17.669540684935043 56-57 15.749323575296263 36.32442777671077 31.218530386603817 16.70771826138915 58-59 14.147862299471248 32.843588695367 33.15199079959928 19.856558205562468 60-61 15.210247310137245 29.796828320492374 35.02425930501584 19.968665064354546 62-63 22.21127798268589 28.986535964742828 32.73384607781916 16.068339974752128 64-65 19.564777896332902 30.30605941483686 36.07188184421857 14.05728084461167 66-67 14.964408694656026 31.519026579483505 37.27068934147662 16.24587538438385 68-69 14.303010291123218 36.02061593995366 34.99172824156904 14.684645527354082 70-71 15.157359368383455 34.43923139392129 31.32574102508517 19.077668212610092 72-73 14.835569063913479 32.68558360935985 30.35988466977344 22.11896265695323 74-75 16.538420499918573 32.72877367761935 32.92341732699122 17.809388495470856 76-77 18.94928012345554 29.4608591346656 34.602818407365106 16.987042334513752 78-79 15.523443808873461 29.863213093985262 37.34634076213859 17.26700233500269 80-81 14.586431372901382 35.418959777525195 34.257728438767764 15.736880410805663 82-83 14.687541417413396 33.776504336613776 32.003221291976196 19.532732953996625 84-85 16.166823341520857 30.172207565542315 32.45696495537294 21.204004137563892 86-87 18.85402394375688 30.087711417084073 33.72397295658229 17.33429168257676 88-89 21.115442076766676 27.10411916021702 34.8746953034609 16.905743459555396 90-91 16.518284381781665 29.53537586434647 37.17004317800932 16.77629657586255 92-93 15.0726535710154 33.35617153813063 34.56045628486804 17.01071860598593 94-95 15.647718796897886 33.980917333908465 31.693199076195867 18.67816479299778 96-97 15.991665449807362 30.849068260190727 29.78107765060038 23.37818863940153 98-99 18.24064464243192 29.92355317131907 31.106535833187316 20.729266353061693 100-101 21.55602851710813 29.481769805639786 32.120535488419 16.841666188833084 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 12091.0 1 7378.0 2 4023.5 3 4956.0 4 3474.0 5 3100.5 6 5997.0 7 11753.5 8 16632.5 9 16363.0 10 10771.5 11 5753.5 12 4655.0 13 4449.0 14 4035.0 15 3389.0 16 2697.0 17 2244.0 18 2039.0 19 1911.5 20 1884.0 21 1838.5 22 1843.5 23 1981.0 24 2109.5 25 2279.5 26 2506.5 27 2711.0 28 2816.0 29 2993.0 30 3299.5 31 3473.5 32 3656.0 33 3916.0 34 4125.5 35 4363.0 36 4677.5 37 4972.0 38 5091.0 39 5041.0 40 4843.5 41 4615.0 42 4452.0 43 4568.5 44 5183.5 45 6100.0 46 9392.5 47 17765.5 48 52907.0 49 58119.0 50 26592.5 51 14957.0 52 8390.5 53 5615.5 54 4444.5 55 3720.0 56 3338.0 57 3045.5 58 2836.0 59 2803.0 60 2812.5 61 2773.5 62 2380.5 63 1549.0 64 914.5 65 694.5 66 596.0 67 481.5 68 379.5 69 297.5 70 259.5 71 222.0 72 178.0 73 197.0 74 187.5 75 121.0 76 75.5 77 57.5 78 38.5 79 17.5 80 10.5 81 9.5 82 7.5 83 4.5 84 3.0 85 3.0 86 2.0 87 0.5 88 0.0 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3910505355887433 2 0.02111358434540027 3 0.03840875450067496 4 0.03211960171693871 5 0.050537834869309156 6 0.04716864587802188 7 0.0307719261204238 8 0.028076574927393974 9 0.03211960171693871 10-11 0.027627349728555677 12-13 0.027515043428846097 14-15 0.021562809544238577 16-17 0.025268917434654578 18-19 0.023472016639301367 20-21 0.030659619820714225 22-23 0.021338196944819424 24-25 0.039194898598641996 26-27 0.032456520616067436 28-29 0.025381223734364154 30-31 0.031221151319262102 32-33 0.028975025325070587 34-35 0.027290430829426948 36-37 0.028638106425941858 38-39 0.014712125261954445 40-41 0.012241386668343776 42-43 0.010893711071828863 44-45 0.009658341775023527 46-47 0.01572288195934063 48-49 0.01370136856456826 50-51 0.0093214228758948 52-53 0.0032568826915777014 54-55 0.003481495290996853 56-57 0.008872197677056496 58-59 0.0029199637924489734 60-61 0.004716864587802188 62-63 0.0056153149854787955 64-65 0.008086053579089465 66-67 0.0040430267895447325 68-69 0.0048291708875117636 70-71 0.015161350460792748 72-73 0.012241386668343776 74-75 0.008647585077637346 76-77 0.00685068428228413 78-79 0.006626071682864979 80-81 0.008310666178508617 82-83 0.010781404772119288 84-85 0.0048291708875117636 86-87 2.246125994191518E-4 88-89 0.021562809544238577 90-91 0.01729517015527469 92-93 0.011342936270667167 94-95 0.021338196944819424 96-97 0.01707055755585554 98-99 0.014599818962244868 100-101 0.11129554301218972 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 445211.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.55503677164978 #Duplication Level Percentage of deduplicated Percentage of total 1 78.49617941505383 21.62965110215827 2 10.944306326664929 6.031415265429031 3 3.9605597415314477 3.2740010794264447 4 1.9587984196660524 2.1589904992859026 5 1.1574927825130628 1.5947378092533337 6 0.6778689378517028 1.1207222105317711 7 0.5010143623546434 0.9663828424464808 8 0.3193377956169724 0.7039491760642607 9 0.2525361088398928 0.626277758872734 >10 1.4533022251980472 7.725202007616545 >50 0.1230093175178901 2.376259938234583 >100 0.11323374261580611 6.184875314394244 >500 0.012219468627604974 2.2889403542582696 >1k 0.021180412287848626 14.215134433079674 >5k 0.0032585249673613265 5.710117165011461 >10k+ 0.005702418692882322 23.393343043936998 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 20346 4.569967947782063 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18466 4.147696260874058 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 16133 3.6236750664291764 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14197 3.188825073953698 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 12450 2.79642686276844 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11818 2.654471699935536 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10805 2.4269391367239357 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 7198 1.6167614906190546 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 6478 1.4550404190372654 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 6412 1.4402159874756015 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 5350 1.2016774068924623 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4855 1.0904941701799822 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4252 0.9550527727302336 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4105 0.9220347206156183 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 4037 0.9067610638551158 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4027 0.9045149378609243 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 3831 0.8604908683747705 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3267 0.733809362302369 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3077 0.6911329684127302 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2881 0.6471088989265764 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2838 0.6374505571515529 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2732 0.6136416216131227 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2706 0.6078016940282248 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2632 0.5911803616712076 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1859 0.41755482232020324 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1816 0.40789648054517974 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1763 0.39599201277596463 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1670 0.37510304102998354 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1573 0.3533156188863258 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1491 0.33489738573395533 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1279 0.28727951465709517 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1218 0.2735781460925269 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1202 0.2699843445018205 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1153 0.25897832713028207 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1028 0.23090175220288806 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1027 0.2306771396034689 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1008 0.22640950021450504 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 945 0.21225890645109846 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 821 0.18440694412312364 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 787 0.1767701157428725 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 778 0.1747486023481001 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 742 0.16666254876901065 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 729 0.16374258497656166 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 725 0.16284413457888508 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 664 0.1491427660143168 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 659 0.14801970301722106 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 625 0.14038287463696988 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 596 0.13386910925381448 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 592 0.13297065885613787 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 521 0.11702316429737808 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 508 0.11410320050492913 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 505 0.11342936270667167 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 499 0.11208168711015676 No Hit TTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCG 488 0.10961094851654608 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 467 0.1048940839287439 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 457 0.10264795793455239 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.3691889912872776E-4 0.0 18-19 0.0 0.0 0.0 6.738377982574555E-4 0.0 20-21 0.0 0.0 0.0 0.001459981896224487 0.0 22-23 0.0 0.0 0.0 0.003593801590706429 0.0 24-25 0.0 0.0 0.0 0.010893711071828863 0.0 26-27 0.0 0.0 0.0 0.02875041272565143 0.0 28-29 0.0 0.0 0.0 0.06446381603329657 0.0 30-31 0.0 0.0 0.0 0.14622280222186784 0.0 32-33 0.0 0.0 0.0 0.2534753184445128 0.0 34-35 0.0 0.0 0.0 0.42215938060829583 0.0 36-37 0.0 0.0 0.0 0.6599118170934681 0.0 38-39 0.0 0.0 0.0 0.9066487575554063 0.0 40-41 0.0 0.0 0.0 1.1765207957575172 0.0 42-43 0.0 0.0 0.0 1.4283115197063865 0.0 44-45 0.0 0.0 0.0 1.680551468854094 0.0 46-47 0.0 0.0 0.0 1.9819815772745957 0.0 48-49 0.0 0.0 0.0 2.3395648355498855 0.0 50-51 0.0 0.0 0.0 2.6499794479471532 0.0 52-53 0.0 0.0 0.0 2.9048024419881813 0.0 54-55 0.0 0.0 0.0 3.1470471304617362 0.0 56-57 0.0 0.0 0.0 3.3888425937364532 0.0 58-59 0.0 0.0 0.0 3.7581057071815387 0.0 60-61 0.0 0.0 0.0 4.3213218002250615 0.0 62-63 0.0 0.0 0.0 4.723602965784763 0.0 64-65 0.0 0.0 0.0 4.996844192978161 0.0 66-67 0.0 0.0 0.0 5.2881667344248005 0.0 68-69 0.0 0.0 0.0 5.58779994204995 0.0 70-71 0.0 0.0 0.0 5.965261415373834 0.0 72-73 0.0 0.0 0.0 6.412914326016203 0.0 74-75 0.0 0.0 0.0 6.814297041178228 0.0 76-77 0.0 0.0 0.0 7.136166896145872 0.0 78-79 0.0 0.0 0.0 7.459272120410322 0.0 80-81 0.0 2.2461259941915183E-4 1.1230629970957592E-4 7.811576982599261 0.0 82-83 0.0 2.2461259941915183E-4 2.2461259941915183E-4 8.175898618857126 0.0 84-85 1.1230629970957592E-4 2.2461259941915183E-4 2.2461259941915183E-4 8.792347897962987 0.0 86-87 2.2461259941915183E-4 2.2461259941915183E-4 2.2461259941915183E-4 9.43316764410583 0.0 88-89 2.2461259941915183E-4 2.2461259941915183E-4 2.2461259941915183E-4 9.82174744110096 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTAAT 65 4.278627E-8 51.352104 1 GTCACTA 65 4.278627E-8 51.352104 1 CATCCGG 20 5.3983997E-4 47.480568 78-79 TGTAGGA 45 9.6008554E-4 42.23816 2 CACTACC 80 2.2517452E-7 41.578186 3 TCACTAC 80 2.2517452E-7 41.578186 2 GCTTATG 115 5.2750693E-11 41.464428 1 CATGGCA 60 8.264155E-5 39.598278 4 ACTACCT 85 3.6232632E-7 39.132412 4 GTTATAG 50 0.001584349 38.147274 1 CTTATGT 130 2.0372681E-10 36.552254 2 GACTTAA 55 0.0025760052 34.550724 9 TGGGAGT 230 0.0 33.052235 6 GTAATGG 105 1.8926257E-6 31.678621 3 TACCTGG 155 1.3460522E-9 30.656727 2 ATGGGTA 80 4.47935E-4 29.698706 5 ATGGGAC 770 0.0 29.00445 5 GGGATCC 85 6.395251E-4 27.94544 9 ATACACG 60 5.3782023E-6 27.709448 92-93 AACGGAG 35 0.008359076 27.163788 94-95 >>END_MODULE