##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938011_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 445211 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.34612576957892 33.0 31.0 34.0 31.0 34.0 2 32.50187888439414 34.0 31.0 34.0 31.0 34.0 3 32.511525995539195 34.0 31.0 34.0 30.0 34.0 4 35.96955600827473 37.0 35.0 37.0 35.0 37.0 5 35.9634195920586 37.0 35.0 37.0 35.0 37.0 6 36.12062145814007 37.0 36.0 37.0 35.0 37.0 7 36.114395196884175 37.0 36.0 37.0 35.0 37.0 8 36.176076062810665 37.0 36.0 37.0 35.0 37.0 9 37.73034358989333 39.0 38.0 39.0 35.0 39.0 10-11 37.7386699789538 39.0 37.5 39.0 35.0 39.0 12-13 37.64424059603199 39.0 37.0 39.0 35.0 39.0 14-15 38.41870034657724 40.0 38.0 41.0 34.0 41.0 16-17 38.402977464617905 40.0 37.5 41.0 34.5 41.0 18-19 38.91625206924357 40.0 38.0 41.0 35.0 41.0 20-21 38.9727398918715 40.0 38.0 41.0 35.0 41.0 22-23 38.84564846780515 40.0 38.0 41.0 35.0 41.0 24-25 38.657482631830746 40.0 38.0 41.0 35.0 41.0 26-27 38.17530451853166 40.0 36.5 41.0 34.5 41.0 28-29 37.86626902749483 39.5 36.0 41.0 33.5 41.0 30-31 37.459199121315514 39.0 35.0 41.0 34.0 41.0 32-33 36.963508314035366 38.0 35.0 41.0 33.0 41.0 34-35 36.67481374000193 38.0 35.0 41.0 33.0 41.0 36-37 36.38135513273482 38.0 35.0 40.0 33.0 41.0 38-39 35.92646520413916 37.5 35.0 40.0 31.5 41.0 40-41 35.51002333724908 37.0 35.0 40.0 30.5 41.0 42-43 35.277935630521256 37.0 35.0 40.0 30.5 41.0 44-45 34.87469312303604 37.0 35.0 40.0 28.5 41.0 46-47 34.12600429908515 36.0 34.5 40.0 23.5 41.0 48-49 33.50409131849842 36.0 33.0 40.0 20.5 41.0 50-51 33.14511658516973 35.0 33.0 40.0 19.0 41.0 52-53 32.349736417114585 35.0 33.0 39.0 12.5 41.0 54-55 32.15549817951488 35.0 33.0 39.0 8.0 41.0 56-57 32.04045385221839 35.0 33.0 39.0 3.5 40.5 58-59 31.725380774509166 35.0 32.5 39.0 2.0 40.0 60-61 31.237655853067423 35.0 31.5 38.5 2.0 40.0 62-63 30.58378499183533 35.0 30.5 38.0 2.0 40.0 64-65 29.794135814254368 35.0 29.0 37.0 2.0 39.5 66-67 29.31366251058487 35.0 28.5 36.0 2.0 39.0 68-69 29.015651005927527 35.0 29.0 36.0 2.0 39.0 70-71 28.46547816653227 34.0 27.5 35.5 2.0 37.5 72-73 27.808753602224563 34.0 26.0 35.0 2.0 37.0 74-75 27.25972516402335 33.0 26.0 35.0 2.0 36.5 76-77 25.755883165510284 31.5 24.0 34.0 2.0 35.0 78-79 26.368773457978353 33.0 24.5 35.0 2.0 35.0 80-81 26.31397921435005 33.0 24.5 35.0 2.0 35.0 82-83 25.90257990031693 33.0 22.5 35.0 2.0 35.0 84-85 25.44248906698172 33.0 20.0 35.0 2.0 35.0 86-87 24.790621750136452 32.5 14.5 35.0 2.0 35.0 88-89 23.922439023294572 31.5 4.5 35.0 2.0 35.0 90-91 23.29043307555294 31.0 2.0 34.5 2.0 35.0 92-93 22.678653492389003 31.0 2.0 34.0 2.0 35.0 94-95 22.031168367358397 30.5 2.0 34.0 2.0 35.0 96-97 21.091781200374655 29.0 2.0 34.0 2.0 35.0 98-99 19.955211124612823 29.0 2.0 34.0 2.0 35.0 100-101 16.595458108627145 21.0 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17069831066593366 1101 2 0.24570784354318675 1101 3 0.23303082284625276 1101 4 -0.02156391687854864 1101 5 0.06663480660205323 1101 6 0.02719869941974906 1101 7 -0.020134073750107007 1101 8 -0.06394308857976938 1101 9 -0.12704451174949583 1101 10-11 0.01644132301558443 1101 12-13 0.14657104589194603 1101 14-15 0.4018615277946722 1101 16-17 0.4503792403141773 1101 18-19 0.08022966791759245 1101 20-21 0.021208115560149565 1101 22-23 0.3449167498757433 1101 24-25 0.37111124586398603 1101 26-27 0.4028643505334557 1101 28-29 0.1860445932654926 1101 30-31 0.4647625921623444 1101 32-33 0.5632438426973465 1101 34-35 0.6340415306981413 1101 36-37 0.6629062767848026 1101 38-39 0.7232062021676384 1101 40-41 0.9956515656518903 1101 42-43 1.3602747330487617 1101 44-45 1.3116050901092464 1101 46-47 1.1002602792200022 1101 48-49 1.2790573748777732 1101 50-51 1.4405088344606725 1101 52-53 1.674173770727485 1101 54-55 1.622294716750389 1101 56-57 1.9079013014503587 1101 58-59 1.788142215971817 1101 60-61 1.3430025707272648 1101 62-63 1.5133591829854538 1101 64-65 1.7063602092149353 1101 66-67 1.6842096863284546 1101 68-69 1.9564528361380304 1101 70-71 1.8541056986183762 1101 72-73 1.8949898692159408 1101 74-75 1.8517328147173124 1101 76-77 1.813401827600746 1101 78-79 2.124538853477901 1101 80-81 2.3990574810700203 1101 82-83 2.7448093441740724 1101 84-85 2.793392791080091 1101 86-87 2.9586013067612527 1101 88-89 3.2637869042160084 1101 90-91 3.64909790754103 1101 92-93 3.3860717982953865 1101 94-95 4.023466592539808 1101 96-97 3.8103478927965355 1101 98-99 4.072886413463568 1101 100-101 3.7644918301531973 1102 1 0.2305367387908106 1102 2 0.2615605614419394 1102 3 0.35400674944877863 1102 4 0.1901777846329793 1102 5 -0.1285302159286985 1102 6 0.04052770251857396 1102 7 0.07653164567776116 1102 8 0.12444241054741667 1102 9 0.3798432675167973 1102 10-11 0.33536309462277814 1102 12-13 0.31085270058280656 1102 14-15 0.39236545883569107 1102 16-17 0.3401274101134959 1102 18-19 0.2843934590379078 1102 20-21 0.38239703557259475 1102 22-23 0.5097193859060027 1102 24-25 0.5171145747317638 1102 26-27 0.3229275448933251 1102 28-29 0.1972084791096833 1102 30-31 0.498051269952299 1102 32-33 0.5866643220001748 1102 34-35 0.6716875069630461 1102 36-37 0.4530184569225568 1102 38-39 0.5047729351410126 1102 40-41 0.32685699468341056 1102 42-43 0.4310199393438481 1102 44-45 0.5668487334602972 1102 46-47 0.6890503846341502 1102 48-49 0.8416597104980141 1102 50-51 0.6827820722582132 1102 52-53 1.0789322924936826 1102 54-55 1.2040786380483794 1102 56-57 1.1149304899373433 1102 58-59 1.0996822909390005 1102 60-61 1.044574987133437 1102 62-63 0.9934401042428433 1102 64-65 0.9404702783633674 1102 66-67 1.0374215024558708 1102 68-69 1.1521083930467846 1102 70-71 1.2353050382576143 1102 72-73 1.5850228872540733 1102 74-75 1.1379308688181808 1102 76-77 1.2963921251690493 1102 78-79 1.0641320820736873 1102 80-81 1.389821902780735 1102 82-83 1.3607107772110574 1102 84-85 1.804485634014366 1102 86-87 1.8050911104121816 1102 88-89 1.7164520282366311 1102 90-91 1.8217761575850098 1102 92-93 2.1615491389901145 1102 94-95 2.054522493160924 1102 96-97 2.4118073171459855 1102 98-99 2.6086988060534537 1102 100-101 2.5250467767695213 1103 1 0.224666564634191 1103 2 0.20364435147969573 1103 3 0.31795145776688827 1103 4 0.15356835825372883 1103 5 0.04520623517348099 1103 6 0.0753468475678929 1103 7 0.10632095270491959 1103 8 0.154310879674199 1103 9 0.47242638772140566 1103 10-11 0.4743778309520934 1103 12-13 0.4664123157332156 1103 14-15 0.6325493584824997 1103 16-17 0.4637390286739702 1103 18-19 0.36581167849960394 1103 20-21 0.2952821896342144 1103 22-23 0.39068394564294096 1103 24-25 0.4453175950703354 1103 26-27 0.6110654087345111 1103 28-29 0.46660014882104406 1103 30-31 0.40920703660678726 1103 32-33 0.46271474216824515 1103 34-35 0.6496499963066071 1103 36-37 0.8820861709646906 1103 38-39 0.8677829217443573 1103 40-41 0.5203870153873495 1103 42-43 0.36609483886886807 1103 44-45 0.27179027529442834 1103 46-47 0.7544930834528074 1103 48-49 0.5585547293751247 1103 50-51 0.61736068631253 1103 52-53 1.0208668924206066 1103 54-55 0.8660777855334629 1103 56-57 1.0563139998630575 1103 58-59 1.0054702582998658 1103 60-61 0.9336110363357264 1103 62-63 1.092459712085983 1103 64-65 1.2465718494265765 1103 66-67 0.9749504270691922 1103 68-69 1.009759714444911 1103 70-71 0.7681268626395372 1103 72-73 1.197503096729168 1103 74-75 1.4734259364104325 1103 76-77 1.5745129387118588 1103 78-79 1.5281102820493295 1103 80-81 1.548660655673192 1103 82-83 1.6003648997296303 1103 84-85 1.9140277117150113 1103 86-87 1.798812946972891 1103 88-89 2.2927551581842636 1103 90-91 2.371849230292355 1103 92-93 2.5235056607292456 1103 94-95 2.735371354444567 1103 96-97 3.136935194383838 1103 98-99 3.2533096938868518 1103 100-101 3.156952147613513 1104 1 0.1845009331642089 1104 2 0.12700529005802252 1104 3 0.23096042947150863 1104 4 0.12140824783274695 1104 5 0.13702818134325412 1104 6 0.1529175860082006 1104 7 0.03461632882638099 1104 8 0.11790646283500195 1104 9 0.038126870813187 1104 10-11 0.21899480817776862 1104 12-13 0.26872425500167907 1104 14-15 0.6381739271505467 1104 16-17 0.5475727070728595 1104 18-19 0.40395867642920535 1104 20-21 0.347870181352647 1104 22-23 0.4364741457809629 1104 24-25 0.4855004798184339 1104 26-27 0.4947093010740602 1104 28-29 0.5359410735967529 1104 30-31 0.511691508656476 1104 32-33 0.540699559283496 1104 34-35 0.481292508383909 1104 36-37 0.6847604290737408 1104 38-39 0.8713543503157766 1104 40-41 0.7435236613500749 1104 42-43 0.8651631618502407 1104 44-45 0.8841263691522627 1104 46-47 0.9626366307267844 1104 48-49 1.163057834965194 1104 50-51 1.1943620665747758 1104 52-53 1.4047350773757543 1104 54-55 1.6331067710407012 1104 56-57 1.5452373953081917 1104 58-59 1.6543142886656312 1104 60-61 1.8413922647691265 1104 62-63 1.9687882145014441 1104 64-65 2.0332523187157427 1104 66-67 2.049001496772437 1104 68-69 2.0521510187927348 1104 70-71 1.5917983602240788 1104 72-73 2.2011780449693354 1104 74-75 2.118180939861091 1104 76-77 2.4992196329837704 1104 78-79 2.153006762380592 1104 80-81 2.4534053071569737 1104 82-83 2.4599577223659317 1104 84-85 2.9778303342132872 1104 86-87 3.1276259214380424 1104 88-89 3.0857330739882656 1104 90-91 3.215638050168131 1104 92-93 3.260582955415238 1104 94-95 3.554522493160924 1104 96-97 3.800340991485289 1104 98-99 4.138678914038678 1104 100-101 3.066251664521726 1105 1 0.16632474809760112 1105 2 -0.0498281063870607 1105 3 0.10932686778837564 1105 4 0.10104036368297642 1105 5 0.11408419015594973 1105 6 -0.1317451103554177 1105 7 -0.10560077560636216 1105 8 -0.04597754923655373 1105 9 0.04677318107057005 1105 10-11 -0.008310778933662277 1105 12-13 0.06235168910726685 1105 14-15 0.15379695490824474 1105 16-17 0.27046620885720074 1105 18-19 0.17032757036704282 1105 20-21 0.12118423691394042 1105 22-23 0.08250613770265858 1105 24-25 -0.16301008279415896 1105 26-27 0.07352440658543458 1105 28-29 -0.04258716030687992 1105 30-31 0.22772304158359447 1105 32-33 0.278714063511984 1105 34-35 0.413585071524345 1105 36-37 0.4621308491685241 1105 38-39 0.5225815870537076 1105 40-41 0.31121101719709543 1105 42-43 0.43928791657501165 1105 44-45 0.5041706621284945 1105 46-47 0.5372834917776572 1105 48-49 0.8171595252127091 1105 50-51 0.8066407451293998 1105 52-53 1.1351101906900354 1105 54-55 1.0677602875128755 1105 56-57 0.9870684394060945 1105 58-59 0.7150421393087925 1105 60-61 0.6136104707888421 1105 62-63 0.5254367508824984 1105 64-65 0.26767805913135945 1105 66-67 0.48003469355488804 1105 68-69 0.5891144254504539 1105 70-71 0.5844372347693891 1105 72-73 0.28637030632084404 1105 74-75 0.37171232935240894 1105 76-77 0.3443749452722038 1105 78-79 0.4807202809182378 1105 80-81 0.40275238737775254 1105 82-83 0.3033057435255806 1105 84-85 0.3061835764361973 1105 86-87 0.40644217443584907 1105 88-89 0.1459646367500369 1105 90-91 0.5079028441369431 1105 92-93 0.8553940217743765 1105 94-95 1.0706332026517842 1105 96-97 1.279987450915904 1105 98-99 1.515169212040906 1105 100-101 1.255942646425865 1106 1 0.09167175533606553 1106 2 0.10311399715786962 1106 3 0.14576684932635686 1106 4 0.06534899467891364 1106 5 -0.13253016744443613 1106 6 0.04129132962611948 1106 7 0.11002214882317674 1106 8 0.15515718976221393 1106 9 0.18711833084936558 1106 10-11 0.308681599853017 1106 12-13 0.17560888535633268 1106 14-15 0.10859314092439121 1106 16-17 0.07218520335236889 1106 18-19 0.15112537743889476 1106 20-21 0.14577869155252188 1106 22-23 0.08266092600854336 1106 24-25 -0.04926037829910257 1106 26-27 -0.031374221145874515 1106 28-29 0.10466717658001556 1106 30-31 0.2343029517500952 1106 32-33 0.3100404022901131 1106 34-35 0.5400133454377283 1106 36-37 0.35814687960834135 1106 38-39 0.14347125394927218 1106 40-41 0.3174813507518266 1106 42-43 0.3261151503109829 1106 44-45 0.02928858267424772 1106 46-47 0.15759622044219412 1106 48-49 0.44302211822640203 1106 50-51 0.6786730311556752 1106 52-53 0.821086933043496 1106 54-55 0.7329419245539981 1106 56-57 0.7060657489039031 1106 58-59 0.7755165232513406 1106 60-61 0.5356478226142158 1106 62-63 0.12579304541931435 1106 64-65 0.17849466594654828 1106 66-67 0.6450474706259541 1106 68-69 0.5661973131810853 1106 70-71 0.2677319179317976 1106 72-73 0.18998222108033147 1106 74-75 0.6294855166406315 1106 76-77 0.8700669897161468 1106 78-79 1.002275256095821 1106 80-81 0.8866825468941393 1106 82-83 1.2540999482514081 1106 84-85 1.2343955744999384 1106 86-87 1.1178267136169886 1106 88-89 1.0413389993036475 1106 90-91 0.7748282418021724 1106 92-93 0.586183385847729 1106 94-95 0.8272919141376356 1106 96-97 1.2576697915402377 1106 98-99 1.440479632952524 1106 100-101 1.801146460409722 1107 1 0.24449257196019403 1107 2 0.2970280511133936 1107 3 0.2562298460452723 1107 4 0.11860956704493475 1107 5 0.17984451356176123 1107 6 -0.052675130454083785 1107 7 0.28631179519575767 1107 8 0.2511515390148631 1107 9 0.3325225415675561 1107 10-11 0.30574229981655776 1107 12-13 0.26794619851710166 1107 14-15 0.46120091525906304 1107 16-17 0.5345838088937427 1107 18-19 0.44080023161316007 1107 20-21 0.37504409439612374 1107 22-23 0.38941334124733373 1107 24-25 0.428307521810261 1107 26-27 0.5575054270495272 1107 28-29 0.6155699290408236 1107 30-31 0.7111759010756558 1107 32-33 0.6402559509594496 1107 34-35 0.4009320475886611 1107 36-37 0.5063764640049939 1107 38-39 0.4839344785209079 1107 40-41 0.2481571619074927 1107 42-43 0.4411978608468914 1107 44-45 0.41282736320651026 1107 46-47 0.4006011420608644 1107 48-49 0.2605007000710984 1107 50-51 0.2850346789865128 1107 52-53 0.5295550792337949 1107 54-55 0.7455649650206411 1107 56-57 0.542154201328259 1107 58-59 0.5080687015233067 1107 60-61 0.6604883257130183 1107 62-63 0.452716122342391 1107 64-65 0.3931034428331692 1107 66-67 0.8522054636992316 1107 68-69 1.1512661247013192 1107 70-71 1.2232550677677452 1107 72-73 0.832954104055176 1107 74-75 0.9272377495972499 1107 76-77 0.6840138544627727 1107 78-79 0.4891794212434668 1107 80-81 0.8796931648307016 1107 82-83 1.0108159070556333 1107 84-85 1.3758294516417529 1107 86-87 1.7212946319545743 1107 88-89 1.9687052497593562 1107 90-91 1.9725360434791703 1107 92-93 2.115092199190787 1107 94-95 1.8962344496826624 1107 96-97 2.014579425153073 1107 98-99 2.5634745290516854 1107 100-101 2.41368291684428 1108 1 0.026398166365794395 1108 2 0.0132706900534032 1108 3 0.046807689254698914 1108 4 -0.027703001965001306 1108 5 -0.174878066489768 1108 6 0.05470433218853543 1108 7 0.12890012791567074 1108 8 -0.14797893577350862 1108 9 -0.1175257653886419 1108 10-11 0.07467402598513218 1108 12-13 0.1367928124295048 1108 14-15 0.08137597046700762 1108 16-17 0.1891833212235241 1108 18-19 0.11006435564701889 1108 20-21 0.1543503144918148 1108 22-23 0.004286132929337327 1108 24-25 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74-75 -0.15048355104866928 2316 76-77 0.07954747160898634 2316 78-79 0.1942862180725946 2316 80-81 0.22242474146018054 2316 82-83 0.6777731149241042 2316 84-85 1.0889368356699372 2316 86-87 1.0439238157478847 2316 88-89 1.0798234969701603 2316 90-91 1.2849626435966073 2316 92-93 1.2981239813399341 2316 94-95 1.4855712817437308 2316 96-97 1.9945687821700986 2316 98-99 2.329182103919564 2316 100-101 2.3090513842547367 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 9.0 5 24.0 6 82.0 7 145.0 8 268.0 9 413.0 10 780.0 11 1243.0 12 2028.0 13 3095.0 14 3544.0 15 4883.0 16 6534.0 17 9507.0 18 8843.0 19 5620.0 20 4745.0 21 4917.0 22 5535.0 23 6122.0 24 6742.0 25 7441.0 26 8053.0 27 9123.0 28 10818.0 29 12413.0 30 14655.0 31 17472.0 32 21365.0 33 26745.0 34 41645.0 35 77884.0 36 56404.0 37 49013.0 38 16594.0 39 503.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 55.540631296171924 17.24979841019202 13.898353814258858 13.311216479377194 2 14.58795726365164 25.759747319992453 39.239983106742024 20.41231230961388 3 24.327092622334035 34.94446253888746 30.286054425588787 10.442390413189726 4 10.699613434770205 18.46842900878925 37.03389240413924 33.79806515230131 5 9.94962103321863 44.81299057643434 30.077367698985675 15.160020691361357 6 21.25149648144363 38.35417363901611 18.548733072632974 21.845596806907285 7 21.390980905682923 45.0343769583411 17.008789091015274 16.565853044960704 8 23.59735046977725 30.110442015134396 19.7288476699812 26.563359845107154 9 28.555448989355607 22.147700753126045 20.099458459022802 29.19739179849554 10-11 22.642746922245856 28.67775054973934 22.87960090833335 25.799901619681453 12-13 33.78330724083637 28.25851113292349 23.100282787262667 14.857898838977473 14-15 28.52580012623228 27.19395971797642 31.254281677676428 13.025958478114871 16-17 15.468620496798147 32.57724988825523 32.86992010529839 19.084209509648232 18-19 14.199896228979068 32.89249367154001 38.10159677096927 14.80601332851165 20-21 17.63321211740051 29.578896298608974 34.976561675250615 17.8113299087399 22-23 15.961357257811827 29.52764615970342 33.4889540022439 21.022042580240853 24-25 17.282088544831144 30.070449979167112 36.716085348583086 15.931376127418664 26-27 20.15564385881761 26.55671937158427 38.5743615546865 14.71327521491162 28-29 15.4868141173511 27.66317543816303 41.75806527691364 15.091945167572229 30-31 14.45898686240906 34.742403040356145 38.083403150416316 12.715206946818475 32-33 13.560536464732454 34.02689960490643 35.622210592280965 16.79035333808015 34-35 15.14645065784301 30.062987597912695 36.2534734155946 18.5370883286497 36-37 18.26341454649186 30.34723173714699 37.60554079338375 13.7838129229774 38-39 20.859067277205536 26.65345854097383 39.00148366786056 13.485990513960067 40-41 14.880961072130953 29.954657720261608 41.73938241501271 13.42499879259473 42-43 13.12845993411735 34.34601980522726 38.05683248892877 14.468687771726618 44-45 13.75770597081474 35.5891466686733 34.17020354207917 16.482943818432783 46-47 13.723043680412209 32.72055272668465 31.41409354216315 22.142310050739987 48-49 16.160651915608554 31.727203505753454 33.25805067709468 18.854093901543315 50-51 19.82868797042301 33.12350773004261 33.12979688282635 13.918007416708033 52-53 14.716954432841955 33.01951209651154 36.881950356123276 15.381583114523226 54-55 11.851459195752126 32.545242592838 37.64653164454607 17.956766566863802 56-57 15.664033458292808 36.596355435961826 30.68365336885207 17.055957736893294 58-59 13.845457547095647 33.415841028186634 32.69270076435667 20.046000660361045 60-61 14.862278784666147 30.523953810665056 34.274534995766054 20.339232408902745 62-63 21.592121488462773 29.892118568498983 32.05569943240396 16.460060510634282 64-65 19.084883347446493 30.992271080453985 35.27147801828796 14.651367553811564 66-67 14.431022593781377 32.19001776685661 36.554465186170155 16.824494453191857 68-69 13.869852629025411 36.46763386589937 34.23146295060905 15.431050554466164 70-71 14.51210774217169 35.05663606694354 30.552142691892158 19.879113498992613 72-73 13.975620548459045 33.07858521015878 29.56407186704731 23.381722374334863 74-75 15.660439656702103 33.14383517029004 31.932724034221977 19.263001138785878 76-77 18.032011787669216 29.736237424502086 34.29194247222104 17.939808315607657 78-79 14.495598716114383 29.78172147588447 37.21437700326362 18.50830280473753 80-81 13.522576935430616 35.40051795665426 34.18895759538735 16.887947512527766 82-83 13.330877505477881 34.196916213967 31.92782112288985 20.54438515766527 84-85 14.523941890305878 30.466932843657517 32.176530377308694 22.83259488872791 86-87 16.75692613174363 30.547980898386783 33.70612626292122 18.988966706948368 88-89 19.01884724321726 27.456082621498567 34.71544952842584 18.80962060685832 90-91 14.574381344073101 29.554760904674087 37.48907791796478 18.381779833288032 92-93 12.688814966386724 33.22402186828268 34.835504962815385 19.251658202515213 94-95 12.965986914069957 34.11988922106597 32.07647609785023 20.83764776701384 96-97 12.887626064104579 31.430560858920508 30.049190269760338 25.632622807214574 98-99 15.059825565855292 30.357852793394592 31.564471677474277 23.01784996327584 100-101 17.80908378274571 30.4799297411789 32.396998277221364 19.313988198854027 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 11379.0 1 6952.5 2 3891.5 3 4994.5 4 3618.5 5 3197.0 6 6174.0 7 11784.0 8 16161.0 9 16034.0 10 10834.5 11 5711.5 12 4659.0 13 4555.0 14 4068.0 15 3360.0 16 2677.0 17 2196.0 18 1990.5 19 1894.0 20 1783.0 21 1780.0 22 1861.5 23 1956.0 24 2016.0 25 2102.5 26 2308.5 27 2555.5 28 2696.0 29 2841.0 30 3138.0 31 3411.5 32 3616.5 33 3828.5 34 4147.5 35 4472.0 36 4871.5 37 5184.5 38 5237.0 39 5307.0 40 5246.5 41 5035.5 42 4822.5 43 4938.5 44 5538.5 45 6045.5 46 8453.0 47 16082.0 48 59097.0 49 63430.5 50 25069.0 51 14686.0 52 6948.5 53 4034.5 54 3334.5 55 3134.0 56 3069.0 57 2853.0 58 2608.0 59 2629.0 60 2644.0 61 2574.5 62 2263.5 63 1415.5 64 737.0 65 560.5 66 466.5 67 361.5 68 290.5 69 231.0 70 195.5 71 162.5 72 148.0 73 175.0 74 144.0 75 84.0 76 54.5 77 36.5 78 28.0 79 13.5 80 5.5 81 2.5 82 0.5 83 0.5 84 0.5 85 0.5 86 1.0 87 1.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014150593763406565 3 0.003593801590706429 4 0.0017969007953532144 5 0.1304999202625272 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010107566973861831 24-25 3.3691889912872776E-4 26-27 0.006289152783736251 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.009882954374442681 36-37 8.984503976766072E-4 38-39 0.00685068428228413 40-41 0.0093214228758948 42-43 0.005727621285188372 44-45 0.004604558288092612 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0017969007953532144 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.002583044893320246 84-85 0.004155333089254309 86-87 0.0029199637924489734 88-89 0.0 90-91 0.002695351193029822 92-93 0.0 94-95 0.0 96-97 2.246125994191518E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 445211.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.24768894171614 #Duplication Level Percentage of deduplicated Percentage of total 1 77.03573971204654 20.22010133356807 2 11.571921014721125 6.074723665050167 3 4.346028503992381 3.422196129138719 4 2.087771220804665 2.1919667834059124 5 1.1948781606761594 1.5681395142338874 6 0.7573745843716931 1.1927599501769848 7 0.4783727112128925 0.8789324685484986 8 0.3808134335788202 0.7996378039523001 9 0.2943813320380906 0.6954146670225472 >10 1.539511349670277 7.690440007047693 >50 0.14462335544798452 2.6444170190347633 >100 0.12237360845598688 6.494803202700263 >500 0.014547911494767673 2.704389782483526 >1k 0.022249746991997617 14.331019676247003 >5k 0.0034230379987688644 6.090267820785575 >10k+ 0.005990316497845512 23.000790176604074 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 20147 4.525270040497651 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17239 3.872096601386758 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 16248 3.6495055153623785 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14338 3.220495450471799 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 12408 2.7869931335928357 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11360 2.5515991294015645 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10660 2.3943703098081586 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 7829 1.7584920408525395 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 6869 1.5428639454101538 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 6566 1.474806327786151 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 5850 1.3139837066020381 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4811 1.0806112158055394 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4284 0.9622403759116464 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 4129 0.9274254230016779 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3976 0.8930596952905476 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 3869 0.8690261471526983 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3640 0.8175898618857126 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3410 0.7659289640193077 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3070 0.6895606802167961 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2974 0.6679978706725576 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2958 0.664404069081851 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2752 0.6181338736015057 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2721 0.6111708830195121 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2652 0.5956726136595907 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1977 0.4440591090516631 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1907 0.42833622709232255 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1771 0.39778891357131785 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1636 0.3674662126497324 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1586 0.35623558267877475 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1478 0.3319774219415064 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1341 0.3012054958210826 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1312 0.2946917304379272 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1308 0.29379328004025057 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1105 0.24819692235816276 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1081 0.24280621997210314 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1039 0.23337249079649872 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1015 0.2279817884104391 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 984 0.2210187978284454 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 957 0.21495425764412832 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 947 0.2127081316499368 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 886 0.19900676308536852 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 789 0.1772193409417108 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 760 0.1707055755585554 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 757 0.17003173776029792 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 682 0.15318579280386152 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 674 0.15138889200850833 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 655 0.14712125261954445 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 631 0.1417305502334848 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 619 0.13903519904045497 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 563 0.12645689347298247 TruSeq Adapter, Index 11 (95% over 21bp) TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 555 0.12465999267762926 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 545 0.12241386668343775 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 521 0.11702316429737808 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 515 0.11567548870086318 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 495 0.11118323671248016 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 482 0.10826327292003117 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 469 0.1053433091275822 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 464 0.10422024613048644 No Hit TTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCG 460 0.10332179573280985 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 453 0.10174950753687577 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.3691889912872776E-4 0.0 18-19 0.0 0.0 0.0 6.738377982574555E-4 0.0 20-21 0.0 0.0 0.0 0.001459981896224487 0.0 22-23 0.0 0.0 0.0 0.003593801590706429 0.0 24-25 0.0 0.0 0.0 0.011118323671248015 0.0 26-27 0.0 0.0 0.0 0.029536556823618464 0.0 28-29 0.0 0.0 0.0 0.06569918533010191 0.0 30-31 0.0 0.0 0.0 0.14700894631983485 0.0 32-33 0.0 0.0 0.0 0.25369993104393196 0.0 34-35 0.0 0.0 0.0 0.4230578310059725 0.0 36-37 0.0 0.0 0.0 0.662831780885917 0.0 38-39 0.0 0.0 0.0 0.9124886851403042 0.0 40-41 0.0 0.0 0.0 1.18370839893893 0.0 42-43 0.0 0.0 0.0 1.4356114291875088 0.0 44-45 0.0 0.0 0.0 1.6876267657357973 0.0 46-47 0.0 0.0 0.0 1.9926506757470053 0.0 48-49 0.0 0.0 0.0 2.35427696081184 0.0 50-51 0.0 0.0 0.0 2.670082275595167 0.0 52-53 0.0 0.0 0.0 2.9282744586274823 0.0 54-55 0.0 0.0 0.0 3.173776029792615 0.0 56-57 0.0 0.0 0.0 3.419052988358329 0.0 58-59 0.0 0.0 0.0 3.7921345159935402 0.0 60-61 0.0 0.0 0.0 4.357372122431835 0.0 62-63 0.0 0.0 0.0 4.761899413985728 0.0 64-65 0.0 0.0 0.0 5.039969812066638 0.0 66-67 0.0 0.0 0.0 5.332752335409502 0.0 68-69 0.0 0.0 0.0 5.639011614717516 0.0 70-71 0.0 0.0 0.0 6.022200709326588 0.0 72-73 0.0 0.0 0.0 6.478164286147467 0.0 74-75 0.0 0.0 0.0 6.891788387977835 0.0 76-77 0.0 0.0 0.0 7.223541197319923 0.0 78-79 0.0 0.0 0.0 7.5585508893535875 0.0 80-81 0.0 0.0 0.0 7.925230957905352 0.0 82-83 0.0 0.0 0.0 8.300670917834466 0.0 84-85 0.0 0.0 0.0 8.946881366363364 0.0 86-87 0.0 0.0 0.0 9.617686894528662 0.0 88-89 0.0 0.0 0.0 10.030524852261063 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGTACT 30 1.3558347E-6 79.26582 4 AGGTAAG 20 0.0020629703 71.33924 2 GGTATGC 20 0.0020740367 71.243095 8 CGTCACT 65 6.6211214E-10 58.53476 1 TAAGGTG 25 0.0049944515 57.071392 5 GGTAAGG 40 5.3514686E-4 47.559494 3 GGGTAAG 40 5.3514686E-4 47.559494 1 GTCACTA 95 1.902663E-8 40.0501 2 TTGGCGC 25 0.00161639 37.99632 80-81 TGCCGTT 25 0.00161639 37.99632 94-95 TGGGACG 65 1.3276348E-4 36.534916 6 AAGGTAA 55 0.0025621566 34.588722 1 ATAGTAC 55 0.0025621566 34.588722 3 GCTTATG 70 2.0362879E-4 33.97106 1 GGGAAGT 340 0.0 33.52616 7 TACCTGG 190 0.0 32.540703 2 ATCCAGG 60 0.0039242506 31.70633 1 GGGCTTA 60 0.003950249 31.663597 7 AGACCGA 30 0.003952353 31.663597 94-95 ACTACCT 125 2.1254345E-7 30.438076 5 >>END_MODULE