##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938010_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 438512 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.36453278359543 33.0 31.0 34.0 31.0 34.0 2 32.52708705805086 34.0 31.0 34.0 31.0 34.0 3 32.43098022403036 34.0 31.0 34.0 30.0 34.0 4 35.94676770533076 37.0 35.0 37.0 35.0 37.0 5 35.984892089612146 37.0 35.0 37.0 35.0 37.0 6 36.14892864961506 37.0 36.0 37.0 35.0 37.0 7 36.13044796949684 37.0 36.0 37.0 35.0 37.0 8 36.18740422154924 37.0 37.0 37.0 35.0 37.0 9 37.7650737037983 39.0 38.0 39.0 35.0 39.0 10-11 37.768090724997265 39.0 38.0 39.0 35.0 39.0 12-13 37.694412011529906 39.0 37.5 39.0 35.0 39.0 14-15 38.48144634582406 40.0 38.0 41.0 34.0 41.0 16-17 38.48870954500675 40.0 37.5 41.0 34.5 41.0 18-19 38.76067017550261 40.0 38.0 41.0 35.0 41.0 20-21 38.8828344492283 40.0 38.0 41.0 35.0 41.0 22-23 38.849891223045205 40.0 38.0 41.0 35.0 41.0 24-25 38.711077461962276 40.0 38.0 41.0 35.0 41.0 26-27 38.216288265771524 40.0 36.5 41.0 34.5 41.0 28-29 37.93731300397709 39.5 36.5 41.0 34.0 41.0 30-31 37.54469204947641 39.0 35.5 41.0 34.0 41.0 32-33 37.08044135622286 38.0 35.0 41.0 33.0 41.0 34-35 36.78296032947787 38.0 35.0 41.0 33.0 41.0 36-37 36.43820807640384 38.0 35.0 40.0 33.0 41.0 38-39 36.066104234319695 37.5 35.0 40.0 32.0 41.0 40-41 35.707897389353086 37.0 35.0 40.0 31.0 41.0 42-43 35.5261589192542 37.0 35.0 40.0 31.0 41.0 44-45 35.13936220673551 37.0 35.0 40.0 29.5 41.0 46-47 34.41444361841865 37.0 35.0 40.0 24.0 41.0 48-49 33.814714306564014 36.5 34.0 40.0 21.5 41.0 50-51 33.49522932097639 36.0 34.0 40.0 20.0 41.0 52-53 32.73250446966104 35.0 33.0 40.0 14.5 41.0 54-55 32.56178964315686 35.0 33.0 39.5 10.0 41.0 56-57 32.53624758273434 35.0 33.0 40.0 7.5 41.0 58-59 32.30418437807859 35.0 33.0 39.5 2.0 41.0 60-61 31.85482609369869 35.0 32.5 39.0 2.0 40.5 62-63 31.291362608092825 35.0 32.0 38.5 2.0 40.0 64-65 30.62012670120772 35.0 31.0 38.0 2.0 40.0 66-67 30.12540021709782 35.0 30.0 37.0 2.0 39.0 68-69 29.77040537089065 35.0 30.0 36.0 2.0 39.0 70-71 29.201170093406795 35.0 29.0 36.0 2.0 38.0 72-73 28.675077306892398 34.0 29.0 35.0 2.0 37.0 74-75 28.183070246652314 34.0 28.0 35.0 2.0 37.0 76-77 26.764170649833986 32.0 26.0 34.5 2.0 35.0 78-79 27.411800589265518 33.0 26.5 35.0 2.0 36.0 80-81 27.515707665924765 34.0 27.0 35.0 2.0 36.0 82-83 27.236348150107638 34.0 26.5 35.0 2.0 35.0 84-85 26.889324579486996 33.5 26.0 35.0 2.0 35.0 86-87 26.365556700842852 33.0 25.0 35.0 2.0 35.0 88-89 25.612400572846354 33.0 21.5 35.0 2.0 35.0 90-91 25.054074916992008 32.5 18.5 35.0 2.0 35.0 92-93 24.739548746670557 32.0 14.0 35.0 2.0 35.0 94-95 24.196276270660782 32.0 3.5 35.0 2.0 35.0 96-97 23.40162184843288 31.0 2.0 35.0 2.0 35.0 98-99 22.588152661728756 31.0 2.0 34.5 2.0 35.0 100-101 19.881711332871163 27.5 2.0 32.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.015289282390163805 1101 2 0.022093544983157187 1101 3 0.18107576051446017 1101 4 0.03196659481375974 1101 5 -0.16958770735976714 1101 6 0.029128042907949236 1101 7 -0.01676413622427475 1101 8 0.04623158984911413 1101 9 0.18821251062193056 1101 10-11 0.14863190231781687 1101 12-13 0.19866259722169843 1101 14-15 0.08843269509617357 1101 16-17 0.07412599653719809 1101 18-19 0.12406664778587384 1101 20-21 0.3116521996002817 1101 22-23 0.19801276947885782 1101 24-25 0.18530966758018508 1101 26-27 0.2786648153696518 1101 28-29 0.4547678525301748 1101 30-31 0.3456405704861467 1101 32-33 0.25542200658288294 1101 34-35 0.235492088592558 1101 36-37 0.2690946855690157 1101 38-39 0.14564641449436522 1101 40-41 0.30355838815938085 1101 42-43 0.3644142547535054 1101 44-45 0.21350440903736967 1101 46-47 0.6778429549251044 1101 48-49 0.8245069655953827 1101 50-51 0.7502740157807395 1101 52-53 0.828184458434599 1101 54-55 0.758194682698182 1101 56-57 0.19092868072251434 1101 58-59 0.5737852999721369 1101 60-61 0.4391656918592872 1101 62-63 0.06097852369862622 1101 64-65 0.084913652500898 1101 66-67 0.09370405997634634 1101 68-69 0.2194756946977563 1101 70-71 0.05250337560036655 1101 72-73 -0.2888721265495491 1101 74-75 -0.03394802335709457 1101 76-77 0.1963095884991013 1101 78-79 0.31322990851066024 1101 80-81 0.7418537676058321 1101 82-83 0.5270918806901044 1101 84-85 0.6204619742161341 1101 86-87 0.8583221712565852 1101 88-89 1.0650068444658523 1101 90-91 1.1625027877871332 1101 92-93 1.2397406377828872 1101 94-95 1.5638757931140255 1101 96-97 1.5267055761882986 1101 98-99 1.4070308638617952 1101 100-101 1.70029900837363 1102 1 -0.07114976692017905 1102 2 -0.08023695833744426 1102 3 0.2870431140065648 1102 4 -0.03419002084780942 1102 5 -0.1608300748397582 1102 6 -0.014027624010097384 1102 7 -0.09870273548960284 1102 8 -0.05864105957798671 1102 9 0.046107759606286436 1102 10-11 0.04358288390245946 1102 12-13 0.0789810344708286 1102 14-15 0.2119092319390461 1102 16-17 0.20392749020007983 1102 18-19 0.027826956249150214 1102 20-21 0.09137341496506224 1102 22-23 0.023145958473435257 1102 24-25 -0.09292153391835001 1102 26-27 -0.19311614651032016 1102 28-29 -0.41678382966507144 1102 30-31 -0.34039830367153456 1102 32-33 -0.20187845319282616 1102 34-35 -0.12496051072142933 1102 36-37 -0.08130488141470948 1102 38-39 0.0628229294431577 1102 40-41 -0.16092454662058486 1102 42-43 -0.17022704721453152 1102 44-45 -0.011198804527232653 1102 46-47 0.10960031985078444 1102 48-49 0.3958938340119573 1102 50-51 0.5482585439632928 1102 52-53 0.33271531692584944 1102 54-55 0.07893689205425147 1102 56-57 0.19926971752889955 1102 58-59 0.5171404462975815 1102 60-61 0.2688723111698579 1102 62-63 0.12624721272969097 1102 64-65 0.3699593868040587 1102 66-67 0.2570916825223861 1102 68-69 -0.046762415599758356 1102 70-71 0.24390021798730643 1102 72-73 0.3830572285481253 1102 74-75 0.33653936321940137 1102 76-77 0.24993792430565875 1102 78-79 0.3746336840233475 1102 80-81 0.7539624109025951 1102 82-83 0.7944094908294979 1102 84-85 0.7277338500523669 1102 86-87 0.75135742072748 1102 88-89 0.6809208007263443 1102 90-91 0.9108485683111311 1102 92-93 0.7933780961233161 1102 94-95 0.9018383055814887 1102 96-97 1.02205004306742 1102 98-99 1.58000626951047 1102 100-101 1.6775139595984818 1103 1 0.19841204251893174 1103 2 0.23332479080712432 1103 3 0.3241845499210072 1103 4 0.18248594122692907 1103 5 0.10135319041098967 1103 6 0.25511966047461243 1103 7 0.27487469023505895 1103 8 0.23568673168232124 1103 9 0.3868397353075892 1103 10-11 0.23338255529722574 1103 12-13 0.062068416331221954 1103 14-15 0.2858906310738263 1103 16-17 0.41443456727157724 1103 18-19 0.491883874293805 1103 20-21 0.24413716588831846 1103 22-23 0.21010048131753223 1103 24-25 0.2090598953637013 1103 26-27 0.1862910074367079 1103 28-29 0.23317245679428567 1103 30-31 0.3645466021936201 1103 32-33 0.29116883279925077 1103 34-35 0.38368045037351806 1103 36-37 0.29494811447018776 1103 38-39 0.3141682513406252 1103 40-41 0.45895230178387436 1103 42-43 0.36770952293063175 1103 44-45 0.1699749794072858 1103 46-47 0.2457679989632524 1103 48-49 0.025685365643980163 1103 50-51 -0.2536286751018437 1103 52-53 0.08295272667246678 1103 54-55 0.37753805875357926 1103 56-57 0.48919600745518466 1103 58-59 0.7084964704756871 1103 60-61 0.732247147244081 1103 62-63 0.4960298509172354 1103 64-65 0.264809479506912 1103 66-67 0.2471769992948154 1103 68-69 0.43061582711590773 1103 70-71 0.14648685370277548 1103 72-73 -0.020026837112631313 1103 74-75 0.06882030562304564 1103 76-77 -0.052141483601975125 1103 78-79 0.2356999929424184 1103 80-81 0.07579958550050492 1103 82-83 0.5416472472697045 1103 84-85 0.26723807820690126 1103 86-87 0.3978957605173541 1103 88-89 0.4772722274458694 1103 90-91 -0.08594828431394319 1103 92-93 0.6366989685852928 1103 94-95 0.5767000049830635 1103 96-97 0.8020578813574666 1103 98-99 1.3090991381738881 1103 100-101 1.2144443646270489 1104 1 0.19449487900615736 1104 2 0.2214148855679241 1104 3 0.25684390070876617 1104 4 0.11540451607067581 1104 5 0.14763908574207818 1104 6 0.1367838789140805 1104 7 0.05469265734240736 1104 8 0.16827407578784914 1104 9 0.07365387165944526 1104 10-11 0.2936953523247894 1104 12-13 0.2224272976353987 1104 14-15 0.30604530142723263 1104 16-17 0.25780563210638974 1104 18-19 0.43374567906928263 1104 20-21 0.2687130465300456 1104 22-23 0.16925109049406473 1104 24-25 -0.030873570461928068 1104 26-27 0.141085212338929 1104 28-29 0.01589949386332279 1104 30-31 0.14627274637980037 1104 32-33 0.2901103345706133 1104 34-35 0.47610822767125427 1104 36-37 0.4193468586736486 1104 38-39 0.4116076937208817 1104 40-41 0.39668812366814876 1104 42-43 0.1541809074751157 1104 44-45 0.2862801725292101 1104 46-47 0.36794893735433476 1104 48-49 0.27693108124638854 1104 50-51 -0.0625949471718883 1104 52-53 0.05554698341948949 1104 54-55 -0.047027740952636066 1104 56-57 0.2450645520561281 1104 58-59 0.2551640012225391 1104 60-61 0.3031665285626701 1104 62-63 0.12468419003991116 1104 64-65 0.3277145170943996 1104 66-67 0.16371405393348937 1104 68-69 0.47659929438167126 1104 70-71 0.7170318002738192 1104 72-73 0.38597563991724115 1104 74-75 0.5199205677273646 1104 76-77 0.6913707143299952 1104 78-79 0.5180885986050221 1104 80-81 0.545391163598957 1104 82-83 0.8025706609624983 1104 84-85 0.9917549133692027 1104 86-87 0.8714189034650168 1104 88-89 0.7554556428668207 1104 90-91 0.5178925521156792 1104 92-93 0.8522365704747479 1104 94-95 1.0937007826552367 1104 96-97 1.1251518644791005 1104 98-99 1.3423374226866933 1104 100-101 1.5316626024795212 1105 1 0.19873642020738913 1105 2 0.11856560598236143 1105 3 0.14352028082856094 1105 4 0.22683401259001812 1105 5 0.1265770815329148 1105 6 0.17894026550951736 1105 7 0.25080727608936826 1105 8 0.21620996699717665 1105 9 0.12772542743085324 1105 10-11 -0.0041676879860403915 1105 12-13 0.10554207827325257 1105 14-15 0.1987035497678491 1105 16-17 -0.07360929423325757 1105 18-19 -0.02095725115574254 1105 20-21 0.08003731397378289 1105 22-23 0.16751885801874522 1105 24-25 0.27424218152123814 1105 26-27 0.33693271112473866 1105 28-29 0.27287699103023755 1105 30-31 0.3661367580359425 1105 32-33 0.0345331935636537 1105 34-35 0.07776760924807036 1105 36-37 0.2959212475355528 1105 38-39 0.19201889657016125 1105 40-41 0.28985632829822805 1105 42-43 0.45210060959588816 1105 44-45 0.6184236078537992 1105 46-47 0.5483277620265099 1105 48-49 0.5196702750297746 1105 50-51 0.26649684496020853 1105 52-53 0.49689391558720786 1105 54-55 0.3664739589016577 1105 56-57 0.34551784653564255 1105 58-59 0.5717254388136084 1105 60-61 0.2463752059141342 1105 62-63 0.17668354247636842 1105 64-65 0.3951987327330606 1105 66-67 0.4346708290670449 1105 68-69 0.19179275398989404 1105 70-71 0.4646520042563367 1105 72-73 0.408624174804725 1105 74-75 0.3242430543713688 1105 76-77 0.5833813991284416 1105 78-79 0.8850547634099435 1105 80-81 0.838616030038974 1105 82-83 0.5311120240837504 1105 84-85 0.498967152320148 1105 86-87 0.8961963034973977 1105 88-89 0.8734255311621091 1105 90-91 0.7570458627842882 1105 92-93 0.7602339802205798 1105 94-95 1.0014796397055825 1105 96-97 0.619242847156773 1105 98-99 0.6866712416926823 1105 100-101 1.0252193452349054 1106 1 0.08839159262118557 1106 2 0.11036637226588653 1106 3 0.04842449538794824 1106 4 0.0833857367279478 1106 5 -0.05035778436746341 1106 6 0.036564786582317765 1106 7 0.16023256344569603 1106 8 0.05184215090522315 1106 9 0.16266795616648722 1106 10-11 0.07809284841242459 1106 12-13 -0.10591386042406015 1106 14-15 0.08008286011267529 1106 16-17 0.1594175256901167 1106 18-19 -0.04578483736263905 1106 20-21 0.10160819519983733 1106 22-23 0.02441540974288614 1106 24-25 0.037498886502071116 1106 26-27 0.20260320920903752 1106 28-29 0.09758963470839177 1106 30-31 0.01969997642674315 1106 32-33 -0.04722925854363069 1106 34-35 0.04408944832852768 1106 36-37 0.0859978758880473 1106 38-39 0.05037138699161403 1106 40-41 0.2787069030108711 1106 42-43 0.4547442877568031 1106 44-45 0.24987954655112077 1106 46-47 0.08959212984259324 1106 48-49 0.4148810029991239 1106 50-51 0.5101750058797521 1106 52-53 0.4128709270814639 1106 54-55 0.27333219644955165 1106 56-57 0.38030711856630006 1106 58-59 0.5067445959017292 1106 60-61 0.6726970449945924 1106 62-63 0.31354178002425925 1106 64-65 0.1283404951851672 1106 66-67 0.009958185388889262 1106 68-69 -0.6623834912208331 1106 70-71 0.014230548317637925 1106 72-73 -0.20267850718761338 1106 74-75 -0.10449257781253962 1106 76-77 0.014109368477100759 1106 78-79 0.11478656417622801 1106 80-81 0.3468152637554489 1106 82-83 0.14873654515654877 1106 84-85 0.3770131293316439 1106 86-87 0.5823265716966297 1106 88-89 0.7973335771391206 1106 90-91 0.5880803455429096 1106 92-93 0.3076669304121502 1106 94-95 0.7496788734220559 1106 96-97 0.8728826939000704 1106 98-99 1.568472007976208 1106 100-101 1.7557940578785818 1107 1 0.0780568036837721 1107 2 0.05774143291605327 1107 3 0.12063216156941792 1107 4 -0.11140320068107457 1107 5 0.07748306958692552 1107 6 0.11175158910535998 1107 7 0.12235774539620081 1107 8 0.01938218001730263 1107 9 -0.01060715300381787 1107 10-11 0.15062074749053522 1107 12-13 -0.023580041889367465 1107 14-15 0.29059232153916525 1107 16-17 0.14383285805305235 1107 18-19 0.21347280455876216 1107 20-21 0.18771688030415845 1107 22-23 0.11488120304711202 1107 24-25 0.03941057985482388 1107 26-27 -0.15860009016020626 1107 28-29 -0.08559813821737094 1107 30-31 -0.007898276848365526 1107 32-33 -0.015720282318497425 1107 34-35 -0.20921477292328916 1107 36-37 -0.41109576797415315 1107 38-39 -0.5154122132024668 1107 40-41 -0.6261402585611862 1107 42-43 -0.9287782741063708 1107 44-45 -1.0718138061902707 1107 46-47 -1.1146145659361508 1107 48-49 -1.2606891085971839 1107 50-51 -1.1601306709761374 1107 52-53 -1.5938830758297478 1107 54-55 -1.3394965274708746 1107 56-57 -1.464952464160536 1107 58-59 -1.4010622939575654 1107 60-61 -1.2804179477273863 1107 62-63 -1.4354004810140708 1107 64-65 -1.2278700822044506 1107 66-67 -1.1770869383374603 1107 68-69 -0.893974951676924 1107 70-71 -1.116332285258295 1107 72-73 -1.2461768089828595 1107 74-75 -1.2758899577252016 1107 76-77 -1.3694520094947578 1107 78-79 -1.3246651582885924 1107 80-81 -1.0644608158175757 1107 82-83 -1.0889150802679985 1107 84-85 -0.7040494616436099 1107 86-87 -0.375572506420788 1107 88-89 -0.7926664228608793 1107 90-91 -0.7547871944862017 1107 92-93 -0.43504534518222115 1107 94-95 -0.4690644550592644 1107 96-97 -0.5728717942124995 1107 98-99 -0.2595241104129222 1107 100-101 -0.14232821286377728 1108 1 0.0263859429036728 1108 2 0.16326656058980404 1108 3 0.17807609614124686 1108 4 0.14782076497653662 1108 5 0.1265819519790483 1108 6 0.1058397395013344 1108 7 0.0024924504513421653 1108 8 -0.020474233275571407 1108 9 0.1473289731156413 1108 10-11 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0.6931960074551853 2316 58-59 0.7733001514572848 2316 60-61 0.8893637116612609 2316 62-63 1.287097335579812 2316 64-65 0.9997849396296097 2316 66-67 0.776624852055555 2316 68-69 0.943505397668055 2316 70-71 0.7440083260954147 2316 72-73 0.7425437150346106 2316 74-75 0.6535074221874595 2316 76-77 0.8472204795882128 2316 78-79 1.0946754530651184 2316 80-81 0.7222597081998927 2316 82-83 0.45918098960099485 2316 84-85 0.07867979599831187 2316 86-87 -0.007895650525593823 2316 88-89 -0.2786664228608764 2316 90-91 -0.36858632112375744 2316 92-93 -0.8891108473656253 2316 94-95 -1.1928766821334982 2316 96-97 -1.2085617505443729 2316 98-99 -1.610305769801574 2316 100-101 -2.4762059421214175 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 8.0 5 26.0 6 55.0 7 121.0 8 235.0 9 387.0 10 700.0 11 1098.0 12 1869.0 13 2698.0 14 3504.0 15 4533.0 16 6252.0 17 8951.0 18 8463.0 19 5194.0 20 4027.0 21 4189.0 22 4501.0 23 4885.0 24 5302.0 25 5693.0 26 6278.0 27 7056.0 28 8259.0 29 9643.0 30 11638.0 31 14146.0 32 17417.0 33 22983.0 34 35989.0 35 79907.0 36 65943.0 37 59436.0 38 26063.0 39 1061.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 55.636561827270405 17.248330718429596 13.929835443499835 13.185272010800162 2 14.68606107047804 25.712181026598884 39.26340492203696 20.338352980886114 3 24.45686631362407 34.986110921058035 30.102811113087665 10.454211652230235 4 10.649651548874376 18.48455686503448 36.92304885613164 33.942742729959505 5 10.070876053562957 44.87699310395155 29.828146094063563 15.223984748421937 6 21.11595577772102 38.2415988616047 18.636890210530158 22.005555150144122 7 21.45756558543438 45.06946218119459 16.932261830919106 16.54071040245193 8 23.590232422373848 30.04455978399679 19.740850877513044 26.62435691611632 9 28.75953223629 22.03086802641661 20.102072463239317 29.107527274054075 10-11 22.719788740102896 28.71301127449192 22.814312949246542 25.752887036158643 12-13 33.87683803407889 28.227391724741853 23.028788265771517 14.866981975407741 14-15 28.612329879227932 27.23996150618455 31.24167639654103 12.906032218046484 16-17 15.554990513372497 32.534685481811216 32.879829970445506 19.030494034370783 18-19 14.162896340351006 32.87903181668917 38.123586127631626 14.8344857153282 20-21 17.65174043127668 29.632826467690737 34.98912230452074 17.72631079651184 22-23 16.002868792644215 29.623932754405807 33.397033604553584 20.976164848396394 24-25 17.205218166663435 30.099667849849432 36.85213332071474 15.842980662772387 26-27 20.11055507910151 26.589100961626187 38.57464463624254 14.725699323029762 28-29 15.50960977122633 27.767427117159848 41.724171744444845 14.99879136716897 30-31 14.409753894990333 34.83724504688583 38.11013153573904 12.642869522384792 32-33 13.522410592522657 34.09308594337945 35.67758575865796 16.706917705439935 34-35 15.1910931894328 30.128566247995785 36.28250363210484 18.397836930466575 36-37 18.25598910791398 30.41596776161475 37.645316186355934 13.682726944115341 38-39 20.80534999275826 26.681880024314005 39.09821511049071 13.414554872437023 40-41 14.83860372607339 30.036790869113005 41.801999890516946 13.322605514296662 42-43 13.12519529334486 34.39705959497586 38.10027757313949 14.377467538539786 44-45 13.634445828030852 35.666767014691864 34.26269288486888 16.436094272408397 46-47 13.639950194524134 32.69704405412925 31.554756377335774 22.108249374010846 48-49 16.11289998905389 31.771650490750535 33.41459298719305 18.70085653300252 50-51 19.8035629583683 33.13352884299631 33.21995749261138 13.84295070602401 52-53 14.639280110920568 33.103883132046555 36.898192067719926 15.35864468931295 54-55 11.877668113985477 32.57402305980224 37.66156912467618 17.886739701536104 56-57 15.588855037034335 36.60880432006422 30.76472251614551 17.037618126755937 58-59 13.802130842485496 33.434318787171165 32.769456708140254 19.994093662203085 60-61 14.820232969679278 30.559597000766225 34.35835279308206 20.261817236472435 62-63 21.5815074615974 29.84388112525997 32.213029518006344 16.36158189513628 64-65 19.114870288612398 30.977487503192613 35.41088955376364 14.496752654431349 66-67 14.450932301661416 32.24510645672918 36.6494379280817 16.654523313527694 68-69 13.873866477846079 36.427664952914576 34.35782185871978 15.340646710519565 70-71 14.527196519137448 35.2421370452804 30.54329185974386 19.68737457583829 72-73 14.065749626007953 33.15211442332251 29.598733900098516 23.183402050571022 74-75 15.668100302842339 33.18586492501915 32.09250830079907 19.05352647133944 76-77 17.996884919910972 29.844337213120735 34.341363520268544 17.81741434669975 78-79 14.57075290984055 29.832022840880068 37.31106560367789 18.28615864560149 80-81 13.51458285586581 35.44935537608478 34.282225209601116 16.753836558448295 82-83 13.29234586370146 34.26208871901995 31.982084705410163 20.463480711868424 84-85 14.583874954959885 30.46205455897176 32.23880154527501 22.715268940793347 86-87 16.674325185657324 30.591013421602042 33.87372451452021 18.86093687822043 88-89 18.960256503812893 27.423422848177477 35.03290673915423 18.583413908855402 90-91 14.533297301118708 29.541284822113745 37.645796488683494 18.279621388084056 92-93 12.561472162075567 33.210417514705995 35.085054781915645 19.143055541302793 94-95 12.89418863587386 34.19077948924943 32.28649647728737 20.628535397589346 96-97 12.868062903637757 31.41966468420477 30.308862699310396 25.40340971284708 98-99 14.972680337140146 30.372372933921994 31.845080636333783 22.80986609260408 100-101 17.83530710306248 30.737102081865263 32.56469198473892 18.862898830333343 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 11205.0 1 6798.0 2 3911.0 3 5005.0 4 3510.5 5 3128.0 6 6082.5 7 11876.0 8 16409.5 9 16172.0 10 10804.0 11 5687.0 12 4669.0 13 4448.0 14 3925.5 15 3273.5 16 2607.0 17 2154.0 18 1980.5 19 1807.5 20 1714.0 21 1725.5 22 1771.5 23 1855.5 24 1960.5 25 2088.5 26 2286.5 27 2555.5 28 2734.5 29 2819.5 30 3030.5 31 3265.5 32 3470.5 33 3786.0 34 4092.5 35 4368.0 36 4759.5 37 5028.0 38 5121.5 39 5235.5 40 5106.0 41 4884.0 42 4788.5 43 4913.0 44 5400.5 45 5815.0 46 7963.0 47 14710.5 48 60625.0 49 64309.0 50 22843.5 51 13770.0 52 6355.5 53 3824.0 54 3358.5 55 3093.5 56 2965.5 57 2788.5 58 2631.5 59 2641.5 60 2648.0 61 2563.0 62 2150.5 63 1294.5 64 690.0 65 520.0 66 442.5 67 365.0 68 266.5 69 200.0 70 189.0 71 164.5 72 141.0 73 185.0 74 160.0 75 90.0 76 58.5 77 37.0 78 25.5 79 9.5 80 2.0 81 0.5 82 0.5 83 1.0 84 0.5 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.007981537563396212 3 0.008665669354544459 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001254241617105119 26-27 0.020067865873681905 28-29 0.0 30-31 0.0 32-33 0.0031926150253584846 34-35 0.013910679753347685 36-37 0.006499252015908344 38-39 0.018585580326194038 40-41 0.020067865873681905 42-43 0.015735031196409677 44-45 0.008665669354544459 46-47 0.0013682635822964936 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.1402196519137448E-4 68-69 1.1402196519137448E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.1402196519137448E-4 82-83 9.121757215309959E-4 84-85 0.0031926150253584846 86-87 5.701098259568723E-4 88-89 0.0 90-91 0.0035346809209326085 92-93 1.1402196519137448E-4 94-95 1.1402196519137448E-4 96-97 0.0 98-99 0.0 100-101 0.002508483234210238 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 438512.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.49287020450956 #Duplication Level Percentage of deduplicated Percentage of total 1 77.12861736026431 20.433584487787655 2 11.66853884037816 6.182661699488341 3 4.218653973757395 3.3529275649347947 4 2.1282826858641863 2.2553726782041954 5 1.1523766322332027 1.5264882272232045 6 0.7305691188365516 1.1612923704455809 7 0.5257559976505932 0.9750149783499721 8 0.34333182161800596 0.727667630976293 9 0.25556259166790807 0.6093527913167368 >10 1.5316543494827128 7.724394576015679 >50 0.14886303447479263 2.706410465237331 >100 0.12218815546485867 6.532133210150814 >500 0.013767679488998163 2.55732080517456 >1k 0.02237247916962201 14.254248110710902 >5k 0.0034419198722495407 5.968373685173396 >10k+ 0.006023359776436696 23.03275671881056 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 19629 4.476274309482979 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17109 3.9016036049184515 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 16105 3.6726474988141717 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14158 3.2286459663589597 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 12395 2.826604517094173 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10881 2.4813460064946913 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10759 2.453524646987996 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 7483 1.7064527310541102 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 6639 1.5139836538110703 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 6473 1.4761283613675338 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 5586 1.2738533951180355 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4697 1.0711223410077717 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4198 0.9573284197467801 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4197 0.9571003758163973 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 3941 0.8987211296384137 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 3761 0.8576732221695187 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3532 0.8054511621118692 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3236 0.7379501587185756 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3019 0.688464625825519 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2940 0.6704491553252818 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2777 0.6332779946728938 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2697 0.6150344802422739 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2666 0.6079651184004087 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2573 0.5867570328748131 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1848 0.42142518334732004 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1825 0.41618017294851684 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1803 0.41116320648009635 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1615 0.3682909475681395 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1601 0.36509833254278107 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1537 0.35050352099828513 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1300 0.2964571094975736 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1290 0.2941766701937461 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1186 0.2704601014339403 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1114 0.2540409384463823 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1088 0.24811179625643084 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1060 0.24172656620571387 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1027 0.23420111650308317 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 986 0.22485131535739047 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 872 0.19885430729375705 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 848 0.1933812529645711 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 812 0.18517167147079214 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 795 0.1812949246542854 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 772 0.17604991425548216 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 740 0.16875250848323423 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 735 0.16761228883132046 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 636 0.14503593972342832 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 635 0.14480789579304557 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 616 0.14047506111577332 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 603 0.13751049002079763 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 591 0.1347739628562046 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 532 0.12131937096362244 TruSeq Adapter, Index 11 (95% over 21bp) GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 523 0.11926697559017768 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 522 0.11903893165979496 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 497 0.11333783340022623 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 489 0.11151348195716423 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 481 0.10968913051410223 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 447 0.10193563688108878 No Hit CATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 441 0.10056737329879228 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.1402196519137447E-4 0.0 12-13 0.0 0.0 0.0 6.841317911482468E-4 0.0 14-15 0.0 0.0 0.0 6.841317911482468E-4 0.0 16-17 0.0 0.0 0.0 7.981537563396213E-4 0.0 18-19 0.0 0.0 0.0 0.0010261976867223701 0.0 20-21 0.0 0.0 0.0 0.0019383734082533658 0.0 22-23 0.0 0.0 0.0 0.005929142189951472 0.0 24-25 0.0 0.0 0.0 0.018243514430619917 0.0 26-27 0.0 0.0 0.0 0.03637300689604846 0.0 28-29 0.0 0.0 0.0 0.07286003575728828 0.0 30-31 0.0 0.0 0.0 0.15472780676469516 0.0 32-33 0.0 0.0 0.0 0.269775969642792 0.0 34-35 0.0 0.0 0.0 0.45141296019265154 0.0 36-37 0.0 0.0 0.0 0.6961040974933411 0.0 38-39 0.0 0.0 0.0 0.9468383989491735 0.0 40-41 0.0 0.0 0.0 1.2288147188674428 0.0 42-43 0.0 0.0 0.0 1.4796630422884665 0.0 44-45 0.0 0.0 0.0 1.7265205969277924 0.0 46-47 0.0 0.0 0.0 2.0390548035173497 0.0 48-49 0.0 0.0 0.0 2.4026708505126426 0.0 50-51 0.0 0.0 0.0 2.7268352975517205 0.0 52-53 0.0 0.0 0.0 2.9780256868683184 0.0 54-55 0.0 0.0 0.0 3.218840077352501 0.0 56-57 0.0 0.0 0.0 3.458400226219579 0.0 58-59 0.0 0.0 0.0 3.832050206151713 0.0 60-61 0.0 0.0 0.0 4.406948954646623 0.0 62-63 0.0 0.0 0.0 4.809446491772175 0.0 64-65 0.0 0.0 0.0 5.071012879921188 0.0 66-67 0.0 0.0 0.0 5.369294340861823 0.0 68-69 0.0 0.0 0.0 5.686047360163462 0.0 70-71 0.0 0.0 0.0 6.051601780567008 0.0 72-73 0.0 0.0 0.0 6.492866785857627 0.0 74-75 0.0 0.0 0.0 6.904258036268107 0.0 76-77 0.0 0.0 0.0 7.219870835917831 0.0 78-79 0.0 0.0 0.0 7.557147808953917 0.0 80-81 0.0 0.0 0.0 7.899897836319189 0.0 82-83 0.0 0.0 0.0 8.265566278687926 0.0 84-85 0.0 0.0 0.0 8.917315831721822 0.0 86-87 0.0 0.0 0.0 9.595176414784543 0.0 88-89 0.0 0.0 0.0 10.01968019119203 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCAACG 35 3.4133682E-6 67.85755 3 GCTTATG 75 3.8198777E-11 57.01985 1 ACGGAGA 25 0.005021358 56.99384 8 GTGTCAA 50 2.8183054E-5 47.51654 1 ATTGATT 45 9.623669E-4 42.21766 6 CTTATGT 105 1.020453E-9 40.728462 2 TTATGTA 110 1.6152626E-9 38.863873 3 GCAGGAC 50 0.0016146785 38.00023 3 TGTCAAC 65 1.3241296E-4 36.55118 2 GTACAGG 65 1.3241296E-4 36.55118 1 TATGTAC 120 3.787136E-9 35.62115 4 ACCACTC 295 0.0 34.61885 94-95 CGTCACT 55 0.0025734939 34.557484 1 AGTACCC 55 0.0025792588 34.54172 5 GGGAAGT 255 0.0 33.52579 7 GGAAGTC 200 0.0 33.246407 8 CTACCTC 160 5.2750693E-11 32.66762 1 ATGGGGT 270 0.0 31.663246 5 TACGTGG 30 0.0039525353 31.663246 9 AGTACTC 190 1.2732926E-11 29.99676 5 >>END_MODULE