##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938009_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 448388 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04484731973202 33.0 31.0 34.0 31.0 34.0 2 32.09351945190326 33.0 31.0 34.0 30.0 34.0 3 32.07735264993711 34.0 31.0 34.0 30.0 34.0 4 35.59613548979901 37.0 35.0 37.0 35.0 37.0 5 35.677821440359686 37.0 35.0 37.0 35.0 37.0 6 35.82349438432786 37.0 36.0 37.0 35.0 37.0 7 35.82240381098513 37.0 36.0 37.0 35.0 37.0 8 35.858341436434515 37.0 37.0 37.0 35.0 37.0 9 37.43139869934075 39.0 38.0 39.0 35.0 39.0 10-11 37.39270676289286 39.0 37.5 39.0 35.0 39.0 12-13 37.27960605546981 39.0 37.0 39.0 34.5 39.0 14-15 38.154249221656244 40.0 38.0 41.0 33.5 41.0 16-17 38.16308197364782 40.0 38.0 41.0 33.5 41.0 18-19 38.40812978937884 40.0 38.0 41.0 34.5 41.0 20-21 38.412295824152295 40.0 38.0 41.0 34.5 41.0 22-23 38.2689846739877 40.0 38.0 41.0 34.5 41.0 24-25 38.13579310775489 40.0 38.0 41.0 34.0 41.0 26-27 37.81724533216767 40.0 36.5 41.0 33.5 41.0 28-29 37.576555349384904 39.5 36.0 41.0 33.5 41.0 30-31 37.20110150137827 39.0 35.0 41.0 33.5 41.0 32-33 36.712758816025406 38.0 35.0 41.0 33.0 41.0 34-35 36.42861539559489 38.0 35.0 41.0 33.0 41.0 36-37 36.038068592379815 38.0 35.0 40.0 32.5 41.0 38-39 35.5666799735943 36.5 35.0 40.0 30.5 41.0 40-41 35.160067843028806 36.5 35.0 40.0 30.5 41.0 42-43 34.91951501824313 36.0 35.0 40.0 30.0 41.0 44-45 34.47574422152243 36.0 35.0 40.0 26.0 41.0 46-47 33.82909332988394 36.0 35.0 40.0 21.5 41.0 48-49 33.35785302015219 36.0 34.0 40.0 17.5 41.0 50-51 32.62949610605101 35.0 33.5 39.5 12.5 40.5 52-53 32.49362717111073 35.0 33.0 39.0 7.5 40.5 54-55 32.63780364327324 35.0 33.5 40.0 2.0 41.0 56-57 32.62226799111484 35.0 34.0 40.0 2.0 41.0 58-59 32.45788691936448 35.0 33.5 40.0 2.0 41.0 60-61 32.03980146658697 35.0 33.0 39.5 2.0 41.0 62-63 31.522280926340578 35.0 33.0 39.0 2.0 40.5 64-65 30.903364942861984 35.0 32.0 38.0 2.0 40.0 66-67 30.500376905715584 35.0 31.0 37.0 2.0 39.5 68-69 30.158092990891816 35.0 31.0 37.0 2.0 39.0 70-71 29.615211602451446 35.0 30.5 36.0 2.0 38.5 72-73 29.010412856722304 35.0 29.5 35.0 2.0 37.0 74-75 28.53692783928205 35.0 29.0 35.0 2.0 37.0 76-77 27.877050679322373 34.0 27.5 35.0 2.0 36.5 78-79 27.36219747183243 34.0 26.5 35.0 2.0 36.0 80-81 27.0436229337092 34.0 26.0 35.0 2.0 35.5 82-83 26.614557035424678 33.5 25.0 35.0 2.0 35.0 84-85 26.148361463732304 33.0 24.0 35.0 2.0 35.0 86-87 25.515088494785765 33.0 20.0 35.0 2.0 35.0 88-89 24.7834698966074 32.5 12.5 35.0 2.0 35.0 90-91 24.066258463652016 31.5 4.5 35.0 2.0 35.0 92-93 23.661339063489656 31.5 2.0 35.0 2.0 35.0 94-95 23.282903422928356 32.0 2.0 35.0 2.0 35.0 96-97 22.397803910898595 31.0 2.0 35.0 2.0 35.0 98-99 21.399831173001953 30.5 2.0 35.0 2.0 35.0 100-101 19.293899479914717 26.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26057808607085065 1101 2 0.008139752205941875 1101 3 0.04703892753952488 1101 4 5.697368258950064E-5 1101 5 0.13767215413050593 1101 6 -0.11302882647595425 1101 7 -0.12127041098973024 1101 8 -0.24257726011929037 1101 9 0.05850910312874191 1101 10-11 -0.0534023077858663 1101 12-13 -0.14893235653052272 1101 14-15 0.02155766399564385 1101 16-17 0.15953263451541488 1101 18-19 0.11359267990414423 1101 20-21 0.144201117607345 1101 22-23 0.08815263233154269 1101 24-25 0.044575982571736006 1101 26-27 0.04542182432919617 1101 28-29 -0.07408568806407345 1101 30-31 0.13618102851354053 1101 32-33 0.3395301558192614 1101 34-35 0.25524841239745655 1101 36-37 0.15603853256281042 1101 38-39 -0.019392718683299393 1101 40-41 -0.10584689410551817 1101 42-43 -0.30035132255075325 1101 44-45 -0.06241996437832853 1101 46-47 0.05822528676577576 1101 48-49 0.20663256658940554 1101 50-51 0.19271326722551407 1101 52-53 0.16998247516839626 1101 54-55 0.4705300245947086 1101 56-57 0.37457139385622185 1101 58-59 0.2619320778132064 1101 60-61 0.18318496014523333 1101 62-63 0.3182903067801348 1101 64-65 0.34113913097158743 1101 66-67 0.47376601749796166 1101 68-69 0.7039328402308627 1101 70-71 0.5714675873292698 1101 72-73 0.6414065210845266 1101 74-75 0.5855641618314742 1101 76-77 0.504903722419872 1101 78-79 0.38084328800428224 1101 80-81 0.8150240689646644 1101 82-83 0.7928212830546819 1101 84-85 0.34506226891513236 1101 86-87 0.13296825083315156 1101 88-89 0.4610572510550135 1101 90-91 0.4801238855136596 1101 92-93 0.6558338210033874 1101 94-95 0.6917818320405011 1101 96-97 0.5770247072287553 1101 98-99 0.5683650009963905 1101 100-101 0.4659526203036428 1102 1 0.502915181083587 1102 2 0.45023415822462454 1102 3 0.4819618737839164 1102 4 0.417275757810728 1102 5 0.37010753818584163 1102 6 0.3736839510995793 1102 7 0.4075853104032987 1102 8 0.3914477368470983 1102 9 0.5707042250799645 1102 10-11 0.5480174228293535 1102 12-13 0.44316811671302503 1102 14-15 0.6867196588991789 1102 16-17 0.47064172321824316 1102 18-19 0.5079926313663421 1102 20-21 0.5805437944669549 1102 22-23 0.6520162411168897 1102 24-25 0.5628261949246038 1102 26-27 0.6233310586454195 1102 28-29 0.6909367606678742 1102 30-31 0.632880458194407 1102 32-33 0.6598128884973349 1102 34-35 0.7658660558873223 1102 36-37 0.7426967051146818 1102 38-39 0.5284048786956603 1102 40-41 0.6226629954709821 1102 42-43 0.7619712111223436 1102 44-45 0.8505214262296121 1102 46-47 1.0106036389075044 1102 48-49 1.1158486811386155 1102 50-51 1.2872527648592964 1102 52-53 0.9066952527439369 1102 54-55 0.7402970431604174 1102 56-57 0.6924939760671265 1102 58-59 0.7215486292026085 1102 60-61 0.5334458508138411 1102 62-63 0.41073661184019983 1102 64-65 0.3047054457392129 1102 66-67 0.4416764652591567 1102 68-69 0.4230992035362853 1102 70-71 0.4488307714088755 1102 72-73 0.550234333243246 1102 74-75 0.4388647321506127 1102 76-77 -0.02164038630479226 1102 78-79 -0.28350569876916865 1102 80-81 0.0582833821548121 1102 82-83 0.3693593245545017 1102 84-85 0.4523429387367557 1102 86-87 0.3451269694352632 1102 88-89 0.5123192338240941 1102 90-91 0.5093375731729246 1102 92-93 1.0686417326645952 1102 94-95 1.01580197522701 1102 96-97 0.9121175357689779 1102 98-99 0.6661140605765361 1102 100-101 0.5088236311033008 1103 1 0.21400760628154103 1103 2 0.35451556807677065 1103 3 0.07831111350082409 1103 4 0.132817766079242 1103 5 -0.024013406387069836 1103 6 -0.010486744717113083 1103 7 0.22802536365815484 1103 8 0.053769928704710424 1103 9 0.21859154902362832 1103 10-11 0.15424683912814174 1103 12-13 0.2687935108690027 1103 14-15 0.262660377839687 1103 16-17 0.012509847386134254 1103 18-19 0.21078150390506067 1103 20-21 0.41575155846387446 1103 22-23 0.3138654457977097 1103 24-25 0.3106014033893203 1103 26-27 0.23523632106571313 1103 28-29 0.4445104415591956 1103 30-31 0.39358398282477225 1103 32-33 0.40758809696205134 1103 34-35 0.2985897613039086 1103 36-37 0.4138479909390824 1103 38-39 0.4901629895498374 1103 40-41 0.4781391358956242 1103 42-43 0.3789742242258356 1103 44-45 0.4223103688404919 1103 46-47 0.25960511042316625 1103 48-49 0.154966472463677 1103 50-51 -0.09721573769833469 1103 52-53 -0.06785102992721193 1103 54-55 0.2665565904941687 1103 56-57 0.17830436076336298 1103 58-59 0.1937825301205507 1103 60-61 0.5397348284608157 1103 62-63 0.5921709892489311 1103 64-65 0.27022993596414224 1103 66-67 0.42752188604256247 1103 68-69 0.17879256770137175 1103 70-71 0.32052862019311945 1103 72-73 0.3439944061183091 1103 74-75 0.10187012770803605 1103 76-77 0.21697288536502768 1103 78-79 0.3083939578771755 1103 80-81 0.1692952243522754 1103 82-83 0.11081051928507435 1103 84-85 0.23531189676352327 1103 86-87 0.01659358004419076 1103 88-89 0.10374590329364608 1103 90-91 0.1315609632647181 1103 92-93 0.0034921285723790163 1103 94-95 -0.08641580909084112 1103 96-97 -0.3312256076687632 1103 98-99 -0.2792646795257703 1103 100-101 -0.38960675219149365 1104 1 0.18510535381543747 1104 2 0.106659894710738 1104 3 0.21631818212982523 1104 4 -0.008164456846323276 1104 5 -0.0604643216162728 1104 6 0.0021260104656732892 1104 7 0.08884194856082672 1104 8 -0.11525470599487164 1104 9 0.03587276440580922 1104 10-11 0.02609892542871961 1104 12-13 -0.036531698816077096 1104 14-15 -0.01125954619892866 1104 16-17 0.3736323878724974 1104 18-19 0.27378725375999835 1104 20-21 0.3210714930526777 1104 22-23 0.3495119088456562 1104 24-25 0.3595252837501377 1104 26-27 0.36264572128727224 1104 28-29 0.30530592607678386 1104 30-31 0.5357425522186645 1104 32-33 0.5684969960494612 1104 34-35 0.42367811916643205 1104 36-37 0.3563810921681707 1104 38-39 0.3335532939228898 1104 40-41 0.47262666029294564 1104 42-43 0.3218739446457377 1104 44-45 0.3986515620672719 1104 46-47 0.5870276983853984 1104 48-49 0.5776520239749914 1104 50-51 0.7856428369706521 1104 52-53 0.8401934918853087 1104 54-55 0.9499901506566388 1104 56-57 0.6439054579833865 1104 58-59 0.6412003704961364 1104 60-61 0.6766763276431789 1104 62-63 0.6449140393095938 1104 64-65 0.7858363082804338 1104 66-67 0.990058152329425 1104 68-69 1.1625927470546458 1104 70-71 1.0529282614865743 1104 72-73 0.9768819938167823 1104 74-75 0.9331936493623054 1104 76-77 1.1029305790929342 1104 78-79 0.8483686374150778 1104 80-81 1.0156817834069756 1104 82-83 0.9224869448798394 1104 84-85 0.4752760261088831 1104 86-87 0.4922173601781772 1104 88-89 0.5616875607288954 1104 90-91 0.870984395242349 1104 92-93 0.9553542375558699 1104 94-95 1.3144044683792266 1104 96-97 1.214733668588032 1104 98-99 0.9759972290040615 1104 100-101 1.0091562377330732 1105 1 0.13341996055700633 1105 2 0.12014304077815297 1105 3 0.12193615965791338 1105 4 0.1379029588840126 1105 5 0.04065927388934654 1105 6 0.08302488687016307 1105 7 0.09783071260577003 1105 8 0.13867787827479106 1105 9 0.058344674518167494 1105 10-11 0.204750610821975 1105 12-13 0.07582785174572138 1105 14-15 0.013459554924672545 1105 16-17 0.044418904726434505 1105 18-19 0.24345017510830758 1105 20-21 0.17837486383919554 1105 22-23 0.18434336951982289 1105 24-25 0.31345786801980324 1105 26-27 0.09972437297266623 1105 28-29 0.29519356652622264 1105 30-31 0.19304592300518664 1105 32-33 0.4516430634651911 1105 34-35 0.43828486073947204 1105 36-37 0.2069428899209882 1105 38-39 0.20433981077682262 1105 40-41 0.12318845804575318 1105 42-43 0.33423349520753476 1105 44-45 0.6301122362245763 1105 46-47 0.6139939905202283 1105 48-49 0.0461913498176898 1105 50-51 -0.054806601231597085 1105 52-53 -0.5305930249686241 1105 54-55 -0.6028188381074031 1105 56-57 -0.6605889240390859 1105 58-59 -0.6250917643353233 1105 60-61 -0.4334360319073838 1105 62-63 -0.29328820788141385 1105 64-65 -0.31416369171956404 1105 66-67 -0.6402789263222601 1105 68-69 -0.5261712978891708 1105 70-71 -0.8616784800864572 1105 72-73 -0.7624438488798475 1105 74-75 -0.6566939910871277 1105 76-77 -0.8240357130418978 1105 78-79 -0.8212942839332378 1105 80-81 -1.075329452548079 1105 82-83 -0.7932433921988107 1105 84-85 -0.8112408278236991 1105 86-87 -0.7605916285858676 1105 88-89 -0.861907944889083 1105 90-91 -0.9874425710497832 1105 92-93 -0.9873423916576165 1105 94-95 -0.6428989024072926 1105 96-97 -0.7661652078164636 1105 98-99 -0.7228791754903199 1105 100-101 -0.5616302791208589 1106 1 0.0704500767993963 1106 2 0.15547684328743117 1106 3 -0.1420723825800536 1106 4 0.11607657016364925 1106 5 0.03041829541481178 1106 6 0.17965410035331075 1106 7 0.3083192218396391 1106 8 0.19518284102383632 1106 9 0.37847960304972617 1106 10-11 -0.2712340519780341 1106 12-13 -0.2283850247073076 1106 14-15 0.10418685853812804 1106 16-17 0.17465405894780162 1106 18-19 0.10425537810780128 1106 20-21 -0.1823963643501898 1106 22-23 -0.006252888397526135 1106 24-25 0.051368902552766826 1106 26-27 0.05151411743354828 1106 28-29 -0.159957257362926 1106 30-31 0.18864375832442448 1106 32-33 0.24734767675904834 1106 34-35 0.023277701761621472 1106 36-37 -0.29181217596588027 1106 38-39 0.02181257025943495 1106 40-41 0.08445232098166144 1106 42-43 -0.023851296211397255 1106 44-45 0.3907038832748313 1106 46-47 0.5361212989872399 1106 48-49 0.8121243223708916 1106 50-51 0.6929134463331366 1106 52-53 0.6916070866628985 1106 54-55 0.19564986866571843 1106 56-57 0.26613226410855617 1106 58-59 0.7075070659604918 1106 60-61 0.4294974502679736 1106 62-63 0.5544779483541724 1106 64-65 0.7077039521273569 1106 66-67 0.538884807994723 1106 68-69 0.14938139946807283 1106 70-71 0.5218995527720374 1106 72-73 0.8279655475819219 1106 74-75 0.6654988402279365 1106 76-77 0.7883942139122624 1106 78-79 1.130740564514845 1106 80-81 0.9846021124772832 1106 82-83 0.9706777862417475 1106 84-85 0.8270487831647237 1106 86-87 0.6490984726105289 1106 88-89 0.5756123793276764 1106 90-91 0.6720982836593699 1106 92-93 0.8043798885374969 1106 94-95 0.6110385354066743 1106 96-97 0.013178107639646441 1106 98-99 0.03133976789308335 1106 100-101 0.15860657384101629 1107 1 0.21332578367230326 1107 2 0.2062775925055007 1107 3 0.2658123310411966 1107 4 0.27495829945932826 1107 5 0.1813418231923407 1107 6 0.16305658997986683 1107 7 0.09989141473640473 1107 8 0.15389070870977406 1107 9 0.126720822590336 1107 10-11 0.12411514996132667 1107 12-13 -0.13797698764073374 1107 14-15 -0.054284862064470474 1107 16-17 0.1458548691070547 1107 18-19 0.211229558762561 1107 20-21 0.12410193736243258 1107 22-23 0.13989840705070833 1107 24-25 0.2779177495993608 1107 26-27 0.2126923435074204 1107 28-29 0.23742630464921177 1107 30-31 0.33004298580530644 1107 32-33 0.27255312920252095 1107 34-35 0.2813591299361704 1107 36-37 0.1715379759389819 1107 38-39 0.08137137401985939 1107 40-41 0.35006849907330917 1107 42-43 0.35736044751300255 1107 44-45 0.4447959044608538 1107 46-47 0.3316591497351098 1107 48-49 0.3113132669233778 1107 50-51 0.1251947974350074 1107 52-53 0.06121311983999078 1107 54-55 0.08149045370107189 1107 56-57 0.12095333900348493 1107 58-59 0.10551572319322844 1107 60-61 0.2957494779065968 1107 62-63 0.11295683850769578 1107 64-65 -0.003638725520673347 1107 66-67 0.10779091376352312 1107 68-69 -0.10196737229823327 1107 70-71 0.08225596907201194 1107 72-73 -0.10680722246369712 1107 74-75 -0.40560070002461046 1107 76-77 -0.6070535693522139 1107 78-79 -0.33053713907799676 1107 80-81 -0.034793763238553765 1107 82-83 -0.45077754297507155 1107 84-85 -0.7755631738538185 1107 86-87 -0.25933515975281907 1107 88-89 -0.21833248682576922 1107 90-91 -0.2246377782855511 1107 92-93 -0.37640481881039634 1107 94-95 -0.7912158402598699 1107 96-97 -0.9258090140545185 1107 98-99 -1.169510720084471 1107 100-101 -1.056054261590905 1108 1 0.02299261816595788 1108 2 -0.029802895002958962 1108 3 0.07186799172975356 1108 4 -0.03579362331710456 1108 5 -0.1358251415119085 1108 6 -0.035827071194809434 1108 7 -0.08277668714035968 1108 8 -0.007145073161439086 1108 9 -0.11758029374848888 1108 10-11 -0.013760507602171401 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-1.1201588462864969 2316 52-53 -1.1228859288008124 2316 54-55 -1.3920592362788895 2316 56-57 -1.2863995789713698 2316 58-59 -1.4046171199981643 2316 60-61 -1.1897631989941537 2316 62-63 -1.04600534378093 2316 64-65 -0.9057773115768732 2316 66-67 -1.1977168722358797 2316 68-69 -1.166353721950351 2316 70-71 -1.0148441997429707 2316 72-73 -1.285983815556481 2316 74-75 -1.3034834130549164 2316 76-77 -0.9200475898308902 2316 78-79 -0.9414186057173168 2316 80-81 -0.9472545176995268 2316 82-83 -0.9284529448967227 2316 84-85 -1.0120461424731637 2316 86-87 -1.1667906288849217 2316 88-89 -1.2206083336616729 2316 90-91 -1.0294513718358722 2316 92-93 -0.8926591772345809 2316 94-95 -1.1636619220168107 2316 96-97 -1.2105344136096008 2316 98-99 -1.4155309463281007 2316 100-101 -0.9559012237100823 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2358.0 3 312.0 4 278.0 5 283.0 6 301.0 7 353.0 8 502.0 9 570.0 10 797.0 11 1251.0 12 2013.0 13 2952.0 14 3424.0 15 4694.0 16 6787.0 17 9974.0 18 8051.0 19 4726.0 20 4305.0 21 4307.0 22 4474.0 23 4919.0 24 5378.0 25 5894.0 26 6547.0 27 7580.0 28 8660.0 29 10021.0 30 11914.0 31 14486.0 32 17583.0 33 23565.0 34 35216.0 35 80072.0 36 68989.0 37 54627.0 38 27895.0 39 2329.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 56.544226894918125 16.924566314010843 13.655870363688924 12.87533642738211 2 14.417108099303377 25.8000455192542 39.775349092515654 20.007497288926775 3 24.278162217407118 35.60910188406412 29.729072338868225 10.383663559660542 4 10.623962220357443 18.201092681533325 37.453132532271596 33.72181256583763 5 9.98627247463756 44.79860715840225 29.584714456311872 15.630405910648317 6 20.89181332249695 38.702626185043115 18.673332231833058 21.732228260626872 7 21.625825745402608 45.22875379396537 17.146045349044815 15.999375111587216 8 23.40671453626144 29.85773001727054 19.473761720092288 27.261793726375732 9 29.33068845640639 22.238610145438482 20.03382705361008 28.396874344545054 10-11 22.654410402572967 28.920996422218405 22.726613293813315 25.697979881395312 12-13 34.22705661847527 28.412162049021937 22.893761239915474 14.46702009258732 14-15 28.721317621016603 27.27657143622208 31.450257842955676 12.55185309980564 16-17 15.273701899365538 32.440520690832564 32.9678618691935 19.317915540608396 18-19 14.06245815740848 32.938376462817 38.470971283708394 14.528194096066127 20-21 17.89143227658309 29.43477421435658 35.24159951074104 17.43219399831929 22-23 16.063115138259647 29.36929497623139 33.75845292030263 20.809136965206328 24-25 17.25624749558833 29.771329774231823 37.116636660233794 15.855786069946056 26-27 20.184440407268703 26.212566230264834 38.93960156943967 14.663391793026795 28-29 15.572886909012999 27.60197203970861 41.901380159977144 14.923760891301244 30-31 14.46200614008363 34.682433133685755 38.13447251860606 12.72108820762455 32-33 13.700197080177931 33.67816784624622 35.933764990257586 16.687870083318266 34-35 15.479321955209938 29.791182828579306 36.290730285353334 18.43876493085742 36-37 18.383762635223956 30.130698823576672 37.96604419566435 13.519494345535016 38-39 21.007168722682213 26.529294038112706 39.27290901099293 13.190628228212148 40-41 14.885610725047385 29.96812696556887 41.89892778532055 13.247334524063191 42-43 13.109017016163849 34.27508107583478 38.12153956511893 14.49436234288245 44-45 13.8268517816274 35.445953361380155 34.40981222311944 16.317382633873002 46-47 13.745272106908663 32.538054589806826 31.7598414350264 21.956831868258117 48-49 16.305372290292176 31.690656910696063 33.57310564444618 18.43086515456558 50-51 20.10915150487646 33.05586290588834 33.32846282201144 13.506522767223764 52-53 14.806647705091741 32.914394066142435 37.18097150186827 15.097986726897553 54-55 11.9833853470718 32.380615643382804 37.99140713707312 17.644591872472276 56-57 15.724658695458196 36.30710716516463 31.221111805121797 16.747122334255376 58-59 14.088757970628157 32.947791200493434 33.06813834456313 19.895312484315276 60-61 15.087571358778218 29.872753272074654 35.066244593017935 19.97343077612919 62-63 22.08060173587953 29.001032783630716 32.947359263712265 15.971006216777491 64-65 19.45945825367897 30.253005302359405 36.28926300104173 13.99827344291989 66-67 14.83578191097237 31.54120424739835 37.37842151224066 16.244592329388617 68-69 14.26227661517974 36.03021627186168 35.029327361162075 14.678179751796502 70-71 15.071398290903412 34.69008679354738 31.331578125348624 18.906936790200586 72-73 14.68232763765728 32.695158805503276 30.464485598681858 22.158027958157586 74-75 16.34914313824572 32.83247942762103 32.996679193591746 17.821698240541505 76-77 18.92136971969622 29.43106061704532 34.827558130326594 16.820011532931876 78-79 15.47149316018745 29.749560034884944 37.68376489691768 17.09518190800993 80-81 14.475072437260076 35.60422377815796 34.41779179557413 15.502911989007828 82-83 14.499650901076741 34.173550124136455 32.1531261641278 19.173672810659003 84-85 16.062679492541477 30.39687717830754 32.49217900460059 21.0482643245504 86-87 18.32084479680246 30.5036640351337 33.986476916745936 17.189014251317904 88-89 20.65705596622793 27.259076132187666 35.31463850542526 16.76922939615914 90-91 16.377276977285458 29.587929314680885 37.48658304453871 16.54821066349495 92-93 14.905080729904535 33.35203682095742 34.907735658366484 16.835146790771557 94-95 15.399523789333685 34.3203063458307 32.05830568083174 18.221864184003874 96-97 15.665357668793789 31.163037172564596 29.978468472488736 23.193136686152886 98-99 17.99844944810392 30.137485986468924 31.489701100445643 20.37436346498151 100-101 21.38483588839003 30.228575450230295 32.22834532197289 16.158243339406784 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 12395.0 1 7450.5 2 4044.5 3 5180.5 4 3629.5 5 3213.0 6 6121.5 7 11918.0 8 17013.5 9 16693.0 10 10757.0 11 5660.0 12 4664.0 13 4509.0 14 4019.5 15 3347.0 16 2719.0 17 2246.0 18 2040.5 19 1919.0 20 1886.0 21 1878.5 22 1924.0 23 2054.5 24 2155.0 25 2260.0 26 2445.5 27 2691.5 28 2879.0 29 3045.0 30 3297.0 31 3441.5 32 3612.5 33 3895.5 34 4056.0 35 4302.0 36 4760.0 37 5066.5 38 5088.0 39 4993.0 40 4760.0 41 4515.0 42 4384.0 43 4497.0 44 5044.5 45 5645.5 46 8191.0 47 15612.5 48 62739.0 49 66180.0 50 21777.0 51 11995.5 52 6566.5 53 4761.0 54 4010.0 55 3429.5 56 3179.5 57 3011.0 58 2806.0 59 2704.0 60 2699.0 61 2744.0 62 2343.0 63 1397.5 64 779.0 65 629.5 66 524.5 67 410.5 68 337.0 69 276.5 70 236.0 71 214.5 72 189.5 73 203.5 74 181.5 75 108.0 76 77.5 77 57.5 78 32.0 79 17.0 80 11.5 81 5.5 82 6.0 83 7.5 84 6.0 85 3.5 86 1.5 87 3.5 88 2.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.480610542655022 2 0.05040277616706959 3 0.0582085158389609 4 0.07047467818050439 5 0.08541709412383917 6 0.11463286261006093 7 0.0686905091126435 8 0.05040277616706959 9 0.05040277616706959 10-11 0.07694229105150004 12-13 0.10849978143928919 14-15 0.05508621997020438 16-17 0.06489914984343917 18-19 0.062445917375130465 20-21 0.0810681820209283 22-23 0.07092072044746961 24-25 0.09556455569729788 26-27 0.07359697404926091 28-29 0.07225884724836526 30-31 0.07772286501868916 32-33 0.07738833331846526 34-35 0.08129120315441091 36-37 0.07471207971667396 38-39 0.042597036495178285 40-41 0.04538480066371089 42-43 0.04382365272933263 44-45 0.020071902013434794 46-47 0.026204983184206537 48-49 0.02575894091724132 50-51 0.024978366950052188 52-53 0.025201388083534795 54-55 0.02430930354960436 56-57 0.024086282416121752 58-59 0.022636645048484793 60-61 0.0278776416853261 62-63 0.01895679634602175 64-65 0.021633049947813055 66-67 0.014830905376593486 68-69 0.019291328046245663 70-71 0.044158184429556546 72-73 0.040701356860576104 74-75 0.034233743989580456 76-77 0.02375175071589784 78-79 0.012712204608508701 80-81 0.015388458210300007 82-83 0.0202949231469174 84-85 0.016838095577936963 86-87 0.009701419306493484 88-89 0.04616537463090002 90-91 0.0582085158389609 92-93 0.036798487024630455 94-95 0.059546642639856553 96-97 0.04638839576438263 98-99 0.03579489192395872 100-101 0.18098164982113704 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 448388.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.179746415474114 #Duplication Level Percentage of deduplicated Percentage of total 1 78.02033897934662 21.205730287079717 2 11.165315435771454 6.0694088438609395 3 4.220613414970688 3.4414560700995454 4 1.9425544395990744 2.111925482662251 5 1.1119208777988334 1.5110863746321836 6 0.7157962723249773 1.167309670016072 7 0.482625005084933 0.9182337676382768 8 0.33323418428940776 0.7245776500762776 9 0.2445894854141289 0.5983092170502595 >10 1.4675350617155143 7.539757256861942 >50 0.14035150075008387 2.6379557139253693 >100 0.11162458539187958 6.056032453778605 >500 0.013132304163750538 2.4465505551475286 >1k 0.020519225255860217 13.179739837523114 >5k 0.004103845051172043 6.878539237477788 >10k+ 0.0057453830716408605 23.513387582170132 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 20571 4.587767736870746 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18801 4.193020330606529 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 16415 3.660891906117024 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14416 3.2150726602852884 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 12313 2.7460592165713624 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11871 2.6474838755720493 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11015 2.456577785310936 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 7309 1.6300614646243878 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 6672 1.487997002595966 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 6465 1.4418316279650658 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 5383 1.2005227615368832 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 5005 1.1162207730804572 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4329 0.9654584868462136 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 4097 0.9137175838782483 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4035 0.8998902736023267 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4000 0.8920845339304352 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 3924 0.8751349277857571 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3300 0.7359697404926091 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3083 0.687574154526883 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2888 0.6440850334977742 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2814 0.6275814696200612 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2753 0.6139771804776221 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2648 0.5905599614619481 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2634 0.5874376655931917 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1843 0.411027949008448 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1830 0.4081286742731742 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1810 0.40366825160352193 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1635 0.36463955324406544 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1625 0.36240934190923935 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1522 0.3394381651605306 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1333 0.29728717093231755 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1296 0.28903538899346104 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1273 0.283905902923361 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1134 0.2529059653692784 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1126 0.25112179630141757 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1076 0.2399707396272871 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1072 0.23907865509335663 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 959 0.21387726700982185 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 917 0.20451037940355227 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 820 0.18287732945573923 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 774 0.1726183573155392 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 740 0.16503563877713054 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 726 0.161913342908374 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 725 0.16169032177489137 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 667 0.14875509603290007 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 665 0.14830905376593484 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 637 0.1420644620284218 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 635 0.1416184197614566 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 608 0.13559684915742615 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 557 0.1242227713498131 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 518 0.11552494714399136 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 513 0.11440984147657832 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 506 0.11284869354220006 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 491 0.10950337653996092 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 489 0.10905733427299572 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.2302113348260881E-4 0.0 14-15 0.0 0.0 0.0 2.2302113348260881E-4 0.0 16-17 0.0 0.0 0.0 5.57552833706522E-4 0.0 18-19 0.0 0.0 0.0 0.0012266162341543485 0.0 20-21 0.0 0.0 0.0 0.0021187007680847835 0.0 22-23 0.0 0.0 0.0 0.007025165704702177 0.0 24-25 0.0 0.0 0.0 0.01694960614467827 0.0 26-27 0.0 0.0 0.0 0.035460360223734805 0.0 28-29 0.0 0.0 0.0 0.07114374158095221 0.0 30-31 0.0 0.0 0.0 0.15053926510076096 0.0 32-33 0.0 0.0 0.0 0.2589275359733088 0.0 34-35 0.0 0.0 0.0 0.4332185517899676 0.0 36-37 0.0 0.0 0.0 0.6663871468460352 0.0 38-39 0.0 0.0 0.0 0.9184010276813831 0.0 40-41 0.0 0.0 0.0 1.1920479584645443 0.0 42-43 0.0 0.0 0.0 1.4350294833938464 0.0 44-45 0.0 0.0 0.0 1.686151279695264 0.0 46-47 0.0 0.0 0.0 2.003398842074275 0.0 48-49 0.0 0.0 0.0 2.3678153741848575 0.0 50-51 0.0 0.0 0.0 2.68773919016566 0.0 52-53 0.0 0.0 0.0 2.9369653068324757 0.0 54-55 0.0 0.0 0.0 3.175932451359091 0.0 56-57 0.0 0.0 0.0 3.411554278883467 0.0 58-59 0.0 0.0 0.0 3.792920417138728 0.0 60-61 0.0 0.0 0.0 4.3689840049243065 0.0 62-63 0.0 0.0 0.0 4.7912745211736265 0.0 64-65 0.0 0.0 0.0 5.061464624387806 0.0 66-67 0.0 0.0 0.0 5.356410073418557 0.0 68-69 0.0 0.0 0.0 5.681351864902718 0.0 70-71 0.0 0.0 0.0 6.066620872993925 0.0 72-73 0.0 0.0 0.0 6.5232566437995665 0.0 74-75 0.0 0.0 0.0 6.949561540451573 0.0 76-77 0.0 0.0 0.0 7.274837863635958 0.0 78-79 0.0 0.0 0.0 7.611376754061215 0.0 80-81 0.0 0.0 0.0 7.961965975895875 0.0 82-83 0.0 0.0 0.0 8.324040786104892 0.0 84-85 0.0 0.0 0.0 8.958981953129879 0.0 86-87 0.0 0.0 0.0 9.636854688350269 0.0 88-89 0.0 0.0 0.0 10.039965387120084 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTACCT 25 0.004999776 57.056095 8 CGTCACT 40 5.253168E-4 47.7387 1 AGATAGT 40 5.370402E-4 47.525513 6 CGACCGT 20 5.379215E-4 47.5149 18-19 TCGACCG 20 5.379215E-4 47.5149 18-19 GTTCGAC 20 5.391067E-4 47.4937 16-17 TAGTCAA 45 9.5723214E-4 42.26377 9 GTGCAGG 70 4.1585117E-6 40.918888 1 TCACTAC 70 4.288442E-6 40.736153 2 GTCACTA 75 6.6790053E-6 38.19096 1 GATAGTC 50 0.0016069781 38.037395 7 AGCGCTC 25 0.0016162368 37.997078 32-33 CGATCCG 25 0.0016175661 37.99072 70-71 GAGGGCC 25 0.0016180094 37.988598 76-77 AGTACTC 215 0.0 37.578312 5 AAAGTAC 180 0.0 36.964283 3 GTCTAAG 80 1.039788E-5 35.804028 1 TACACGG 55 0.002571173 34.564007 2 GTACACG 70 1.9918002E-4 34.09907 1 ATAGTCA 60 0.0039294325 31.69783 8 >>END_MODULE