##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938009_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 448388 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.39087575938696 33.0 31.0 34.0 31.0 34.0 2 32.53812992319152 34.0 31.0 34.0 31.0 34.0 3 32.543937393507406 34.0 31.0 34.0 31.0 34.0 4 35.98885563395987 37.0 35.0 37.0 35.0 37.0 5 36.02548462492306 37.0 35.0 37.0 35.0 37.0 6 36.16709635405051 37.0 36.0 37.0 35.0 37.0 7 36.14935502288197 37.0 36.0 37.0 35.0 37.0 8 36.20508131350527 37.0 37.0 37.0 35.0 37.0 9 37.78973121492993 39.0 38.0 39.0 35.0 39.0 10-11 37.81160735791323 39.0 38.0 39.0 35.0 39.0 12-13 37.750999134678 39.0 38.0 39.0 35.0 39.0 14-15 38.59919087932772 40.0 38.0 41.0 34.0 41.0 16-17 38.62053957733035 40.0 38.0 41.0 35.0 41.0 18-19 38.897502832368396 40.0 38.0 41.0 35.0 41.0 20-21 38.962410903057176 40.0 38.0 41.0 35.0 41.0 22-23 38.795068110654164 40.0 38.0 41.0 35.0 41.0 24-25 38.65292447612336 40.0 38.0 41.0 35.0 41.0 26-27 38.25564466488844 40.0 36.5 41.0 34.5 41.0 28-29 37.99757687538471 40.0 36.5 41.0 34.0 41.0 30-31 37.622438602281946 39.0 35.5 41.0 34.0 41.0 32-33 37.179313451742686 38.0 35.0 41.0 33.0 41.0 34-35 36.89266438887749 38.0 35.0 41.0 33.0 41.0 36-37 36.530698859023886 38.0 35.0 41.0 33.0 41.0 38-39 36.14545995878569 38.0 35.0 40.5 32.5 41.0 40-41 35.84676663960677 37.5 35.0 40.0 31.5 41.0 42-43 35.68763214002159 38.0 35.0 40.0 31.0 41.0 44-45 35.33593784847052 38.0 35.0 41.0 30.5 41.0 46-47 34.654840227659975 37.0 35.0 40.0 25.0 41.0 48-49 34.09504714666762 37.0 35.0 40.0 22.5 41.0 50-51 33.773697110538194 37.0 35.0 40.0 20.0 41.0 52-53 33.08506472073294 35.0 33.5 40.0 18.5 41.0 54-55 32.98834380045854 35.0 33.5 40.0 15.0 41.0 56-57 32.97972849407209 35.0 34.0 40.0 11.0 41.0 58-59 32.779720911353564 35.0 33.0 40.0 4.5 41.0 60-61 32.36411656868604 35.0 33.0 39.5 2.0 41.0 62-63 31.84348599873324 35.0 33.0 39.0 2.0 40.0 64-65 31.20905889542093 35.0 32.0 38.0 2.0 40.0 66-67 30.777342390964968 35.0 31.5 37.5 2.0 39.5 68-69 30.450580747031587 35.0 31.0 37.0 2.0 39.0 70-71 29.92990334264075 35.0 31.0 36.5 2.0 38.5 72-73 29.444961729573492 35.0 30.5 35.5 2.0 37.0 74-75 29.05226054220898 35.0 30.0 35.0 2.0 37.0 76-77 27.605144428486042 33.0 27.5 35.0 2.0 35.5 78-79 28.22515544573004 34.0 29.0 35.0 2.0 36.0 80-81 28.255065925047056 34.0 29.0 35.0 2.0 36.0 82-83 28.01914301899248 34.0 29.0 35.0 2.0 35.0 84-85 27.749393382516928 34.0 29.0 35.0 2.0 35.0 86-87 27.254077941425727 34.0 27.0 35.0 2.0 35.0 88-89 26.629269739600524 33.5 25.5 35.0 2.0 35.0 90-91 26.20116952282398 33.0 24.5 35.0 2.0 35.0 92-93 26.083900550416157 33.0 24.0 35.0 2.0 35.0 94-95 25.911985824776757 33.0 23.5 35.0 2.0 35.0 96-97 25.342350821163812 33.0 19.0 35.0 2.0 35.0 98-99 24.74320231585145 33.0 2.0 35.0 2.0 35.0 100-101 22.889170762821486 31.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.022691433063968702 1101 2 0.14451878660909045 1101 3 0.20365396972274397 1101 4 -0.15646381913844465 1101 5 -0.04770208779137164 1101 6 -0.1321575089397129 1101 7 0.013607476826152265 1101 8 -0.009475443861717281 1101 9 -0.029130759963827302 1101 10-11 0.012260253757844453 1101 12-13 0.06651714266665465 1101 14-15 0.027048982170079228 1101 16-17 0.024940569864050133 1101 18-19 0.15863988470682244 1101 20-21 0.30760780350524897 1101 22-23 0.2877379856530595 1101 24-25 0.27871375839072954 1101 26-27 0.3678060448985718 1101 28-29 0.2536334198974046 1101 30-31 0.2034088862812098 1101 32-33 0.39792964008244525 1101 34-35 0.711845875244876 1101 36-37 0.8519935824351776 1101 38-39 1.028388244227294 1101 40-41 0.6895027246564993 1101 42-43 0.7918634619856704 1101 44-45 0.9416565103156032 1101 46-47 1.0353873525029442 1101 48-49 0.9027003096456667 1101 50-51 0.938004275784408 1101 52-53 0.811441566584989 1101 54-55 0.8549809423275079 1101 56-57 1.0100213125242377 1101 58-59 0.9735526576076907 1101 60-61 1.0666687966110047 1101 62-63 0.969928030376014 1101 64-65 1.3300421304268006 1101 66-67 0.8660708675017865 1101 68-69 0.8630177489464508 1101 70-71 0.7727238943210928 1101 72-73 0.24886949717422624 1101 74-75 0.43952627493744956 1101 76-77 0.1524195316129493 1101 78-79 0.36152716973264987 1101 80-81 0.6674533178489348 1101 82-83 0.6122786739749877 1101 84-85 0.6345759967662765 1101 86-87 0.7019084128409432 1101 88-89 0.77495622976733 1101 90-91 0.5740871037037074 1101 92-93 0.6334082568450121 1101 94-95 0.2346556002493685 1101 96-97 0.3620510290022665 1101 98-99 0.2750794110434427 1101 100-101 0.5167908853466265 1102 1 0.21344495815068854 1102 2 0.17805840558736463 1102 3 0.3877093028133842 1102 4 0.18902823279699987 1102 5 0.1959819311383697 1102 6 0.07547505992329206 1102 7 0.15899031871427383 1102 8 0.2831104073699322 1102 9 0.5383489148329232 1102 10-11 0.20554990307223875 1102 12-13 0.4075497843363536 1102 14-15 0.36297908773978804 1102 16-17 0.46380721226181265 1102 18-19 0.5516686433526203 1102 20-21 0.5188503711548123 1102 22-23 0.4839034995470044 1102 24-25 0.40477855635214866 1102 26-27 0.3094818124025167 1102 28-29 0.321258708090582 1102 30-31 0.417770007504366 1102 32-33 0.4357223836815223 1102 34-35 0.5488984174120901 1102 36-37 0.637074276525702 1102 38-39 0.6860390147648516 1102 40-41 0.574874645539893 1102 42-43 0.6130016733677834 1102 44-45 0.5082321686095028 1102 46-47 0.6955742230283661 1102 48-49 0.865926859821613 1102 50-51 0.8147546701540236 1102 52-53 0.6047918881479148 1102 54-55 0.7978580203520238 1102 56-57 0.8202870569727239 1102 58-59 0.8150585428157981 1102 60-61 0.6569369679494415 1102 62-63 0.5532795653839067 1102 64-65 0.8076783396247151 1102 66-67 0.6630311878512565 1102 68-69 0.7523637021074769 1102 70-71 0.7196356768717536 1102 72-73 1.010142400948041 1102 74-75 0.7873966790146234 1102 76-77 0.9867054192479472 1102 78-79 0.9277084584112103 1102 80-81 1.1718277869667624 1102 82-83 1.1587960869103107 1102 84-85 1.1009575038649295 1102 86-87 0.9846896287128004 1102 88-89 0.6770008845422346 1102 90-91 0.7916274507489724 1102 92-93 0.7732274535444432 1102 94-95 0.8005153260108067 1102 96-97 0.9436370015784092 1102 98-99 0.8420494389526745 1102 100-101 0.5179011874944237 1103 1 0.09502298351681304 1103 2 0.12347218177684027 1103 3 -0.014610086157254898 1103 4 0.09993146312423562 1103 5 0.130884881176911 1103 6 0.08864862869913281 1103 7 0.03969244190115262 1103 8 -0.06637456214867399 1103 9 -0.006252024134212775 1103 10-11 -0.053244154807373434 1103 12-13 0.1048029551022438 1103 14-15 0.13365037916996414 1103 16-17 0.011211038202532109 1103 18-19 -0.2040247208124768 1103 20-21 -0.25304718332630216 1103 22-23 -0.18929739737682638 1103 24-25 -0.11296722179934449 1103 26-27 -0.023290943536103725 1103 28-29 -0.01718989396953674 1103 30-31 -0.19411773687235012 1103 32-33 -0.293115819667932 1103 34-35 -0.16489178649793246 1103 36-37 -0.07219627213942914 1103 38-39 -0.04849255274968556 1103 40-41 0.18401205707377244 1103 42-43 0.056663645198767654 1103 44-45 0.2608423571036482 1103 46-47 0.5769595499150597 1103 48-49 0.5918220318487997 1103 50-51 0.4253110432182794 1103 52-53 0.42094002072446557 1103 54-55 0.32898758380238036 1103 56-57 0.4502709403726257 1103 58-59 0.5003819259003777 1103 60-61 0.2871485859159435 1103 62-63 0.563790391534841 1103 64-65 0.6738930407668917 1103 66-67 0.7786152394243793 1103 68-69 0.5882897058913734 1103 70-71 0.5278481357043532 1103 72-73 0.5726642985025201 1103 74-75 0.5505877658340488 1103 76-77 0.3560208141046459 1103 78-79 0.476058258705514 1103 80-81 0.5453475122838363 1103 82-83 0.3613397068388373 1103 84-85 0.5184475185800856 1103 86-87 0.7679739115240984 1103 88-89 0.6652287592531891 1103 90-91 0.6635179980126509 1103 92-93 0.46107343490524855 1103 94-95 0.4296057891878782 1103 96-97 0.5590108366285094 1103 98-99 0.7754458372429127 1103 100-101 0.38910853105829446 1104 1 0.13729760502865673 1104 2 0.05690574193931752 1104 3 0.0526262909066304 1104 4 -0.033169765973191545 1104 5 -0.015857746876051237 1104 6 0.043013770862977196 1104 7 0.15446798655484884 1104 8 0.13640013848961274 1104 9 0.07692569385913828 1104 10-11 0.13400593750681367 1104 12-13 0.18707071898893446 1104 14-15 0.19431398628078966 1104 16-17 0.2502625758931032 1104 18-19 0.25936611107021434 1104 20-21 0.23438226226107872 1104 22-23 0.1770501259654722 1104 24-25 0.2751374361106613 1104 26-27 0.170930188499554 1104 28-29 0.2973166207743603 1104 30-31 0.5389254661661056 1104 32-33 0.6068498922063128 1104 34-35 0.5964443953873797 1104 36-37 0.5596943223639173 1104 38-39 0.38902129437801847 1104 40-41 0.5438052732999736 1104 42-43 0.6594436209333239 1104 44-45 0.5306397934068627 1104 46-47 0.6542555355667972 1104 48-49 0.47963919701206237 1104 50-51 0.5259598800595526 1104 52-53 0.4534530765877349 1104 54-55 0.5322897940677151 1104 56-57 0.5666395641000079 1104 58-59 0.3447639483722895 1104 60-61 0.39052464752906957 1104 62-63 0.049771823195964515 1104 64-65 -0.004885246936861876 1104 66-67 -0.04031444354233571 1104 68-69 -0.028020891777032375 1104 70-71 0.0867551357571017 1104 72-73 -0.05158268150486478 1104 74-75 0.07701599814928883 1104 76-77 -0.10204744564109092 1104 78-79 0.2960297731856514 1104 80-81 0.4241811884984301 1104 82-83 0.2476719324019534 1104 84-85 0.12545020887918312 1104 86-87 -0.10054431393351848 1104 88-89 -0.3343887962672021 1104 90-91 -0.3125119645341634 1104 92-93 0.0424655328110326 1104 94-95 -0.20976752937518484 1104 96-97 -0.14621150867929344 1104 98-99 0.12079056478419403 1104 100-101 0.5001835956782266 1105 1 0.16201670615225083 1105 2 0.1782540565460593 1105 3 0.06386224596280954 1105 4 0.09042573964478606 1105 5 0.12796247784304882 1105 6 -0.013166004417925592 1105 7 -0.10620617074852845 1105 8 -0.15124031094859447 1105 9 -0.010714755579066093 1105 10-11 -0.09745473665049076 1105 12-13 -0.07360343831443572 1105 14-15 -0.12141635079785829 1105 16-17 0.15588055342118423 1105 18-19 -0.03726310241293618 1105 20-21 0.02539349821613257 1105 22-23 0.18154450798794386 1105 24-25 0.06727226757133309 1105 26-27 0.07205378400517048 1105 28-29 -0.011672143270587299 1105 30-31 0.05240861223352766 1105 32-33 0.014715060745643882 1105 34-35 -0.06872414393846782 1105 36-37 0.016997693150436533 1105 38-39 -0.1323270202287219 1105 40-41 0.12133336318760968 1105 42-43 -1.0694086442697426E-4 1105 44-45 0.12389822037315668 1105 46-47 -0.21428379027589273 1105 48-49 -0.18328215130254222 1105 50-51 -0.07066933342359505 1105 52-53 -0.19935591217631554 1105 54-55 -0.14748548683116525 1105 56-57 -0.3805514471359501 1105 58-59 -0.1653484111782788 1105 60-61 -0.2701495097742992 1105 62-63 0.04303025016225703 1105 64-65 -0.05432344918405008 1105 66-67 -0.29761781432885215 1105 68-69 -0.29993100413658524 1105 70-71 -0.3233572237934581 1105 72-73 -0.4538298725160992 1105 74-75 -0.42186040634509325 1105 76-77 -0.5705867714837893 1105 78-79 -0.5972286537806397 1105 80-81 -0.8758188115015706 1105 82-83 -0.5658112136654587 1105 84-85 -0.6824149596601465 1105 86-87 -0.5657128532593596 1105 88-89 -1.0411303693009053 1105 90-91 -1.172062526331917 1105 92-93 -1.0462985121327897 1105 94-95 -1.5007787653302422 1105 96-97 -1.0776721828365936 1105 98-99 -0.8881981992607528 1105 100-101 -1.004310786344245 1106 1 -0.36262716503468084 1106 2 -0.1628773046304417 1106 3 -0.38961895868688856 1106 4 -0.17294698828978738 1106 5 -0.10329920099792389 1106 6 -0.2511692562926058 1106 7 -0.15176153755513866 1106 8 -0.17951706004461698 1106 9 -0.445092409861104 1106 10-11 -0.11712129815703065 1106 12-13 -0.39394221570663035 1106 14-15 -0.5786008097490054 1106 16-17 -0.4792421199110066 1106 18-19 -0.31392240787074854 1106 20-21 -0.12334094010745389 1106 22-23 -0.238585566999312 1106 24-25 -0.17672268474259312 1106 26-27 -0.05331811882687276 1106 28-29 -0.07461290576239321 1106 30-31 -0.11669857829235752 1106 32-33 -0.08174427868698331 1106 34-35 -0.043213915093851085 1106 36-37 0.199609870693088 1106 38-39 -0.11656513511211841 1106 40-41 0.06038983417554533 1106 42-43 -0.0723834958186842 1106 44-45 0.08961521020497543 1106 46-47 0.2033297219914374 1106 48-49 0.03417847431931875 1106 50-51 0.1742235245859689 1106 52-53 -0.11056759951098627 1106 54-55 -0.4782909325078677 1106 56-57 -0.2517286743060865 1106 58-59 -0.26473686459637946 1106 60-61 -0.30650983690318867 1106 62-63 -0.16638077223684888 1106 64-65 -5.51031876309338E-4 1106 66-67 -0.09033749787776557 1106 68-69 0.08942031966762443 1106 70-71 -0.11277892399002809 1106 72-73 0.3749006829720436 1106 74-75 0.36076633183045814 1106 76-77 -0.013831851203253365 1106 78-79 0.29239689527656054 1106 80-81 0.1208273893866263 1106 82-83 0.2549352614479936 1106 84-85 0.2581655242382226 1106 86-87 0.3091069589084796 1106 88-89 0.3339563878034646 1106 90-91 0.176836932497892 1106 92-93 0.172693406715954 1106 94-95 0.2196158278214071 1106 96-97 -0.21051126357530592 1106 98-99 -0.46354462098521765 1106 100-101 -0.7942663764138942 1107 1 0.00357109097205921 1107 2 0.040065796021089284 1107 3 0.08142949851537651 1107 4 0.07177218936271856 1107 5 -0.024151839840754974 1107 6 -0.07658621048813785 1107 7 -0.04447368904774862 1107 8 -0.1455244267581861 1107 9 0.04733923295187026 1107 10-11 0.016779027161341276 1107 12-13 0.042956774282885135 1107 14-15 0.22643669596422455 1107 16-17 0.012866893110683009 1107 18-19 0.17217090383444145 1107 20-21 0.1815173268328465 1107 22-23 0.077723817046639 1107 24-25 0.16646803427374124 1107 26-27 0.3035261137175169 1107 28-29 0.36220542677929757 1107 30-31 0.4947812170169641 1107 32-33 0.3808463489175864 1107 34-35 0.21608633673662325 1107 36-37 0.06481111102539927 1107 38-39 0.08050341475659906 1107 40-41 0.13913838906294274 1107 42-43 0.21950236493071884 1107 44-45 0.24482926610955502 1107 46-47 -0.06535143911732888 1107 48-49 -0.3575769902227677 1107 50-51 -0.20220926535515105 1107 52-53 -0.10005524547855771 1107 54-55 -0.054415879856478 1107 56-57 -0.2265792339791588 1107 58-59 -0.04941060521995411 1107 60-61 -0.10156822393347653 1107 62-63 0.08701132154087432 1107 64-65 0.09645747300346486 1107 66-67 -0.08600888151147146 1107 68-69 -0.26489440569376654 1107 70-71 -0.19807865602806274 1107 72-73 -0.16458561870473432 1107 74-75 0.06899697628346857 1107 76-77 0.04568438334860403 1107 78-79 0.11994230990065091 1107 80-81 0.2746590662549693 1107 82-83 -0.046020081211732844 1107 84-85 0.18256709078509914 1107 86-87 0.4226502405911674 1107 88-89 0.5060816219341149 1107 90-91 0.09470748625608039 1107 92-93 0.39613470153684816 1107 94-95 0.08365873758364728 1107 96-97 0.2372003053912941 1107 98-99 0.13753260404010348 1107 100-101 0.06966002814593963 1108 1 -0.0015158028976287596 1108 2 0.1118725982225186 1108 3 0.01972703840321799 1108 4 0.07042667989207274 1108 5 -0.05746839047531438 1108 6 0.049891679753017115 1108 7 0.06952134558827083 1108 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-0.17902725342065295 2316 82-83 -0.18410915872463107 2316 84-85 -0.19962135091062194 2316 86-87 -0.019254528826497364 2316 88-89 -0.16376676012416524 2316 90-91 -0.10612712239292321 2316 92-93 -0.421261130153038 2316 94-95 -0.3882461896050309 2316 96-97 -0.22599960625694138 2316 98-99 -0.0659249476693482 2316 100-101 -0.19750940214293777 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 6.0 5 27.0 6 64.0 7 122.0 8 249.0 9 402.0 10 684.0 11 1147.0 12 1788.0 13 2658.0 14 3451.0 15 4407.0 16 5989.0 17 8669.0 18 8523.0 19 4879.0 20 3712.0 21 3916.0 22 4143.0 23 4503.0 24 4894.0 25 5143.0 26 5623.0 27 6050.0 28 7340.0 29 8376.0 30 10052.0 31 11906.0 32 14712.0 33 19416.0 34 29521.0 35 72887.0 36 72327.0 37 74737.0 38 43417.0 39 2647.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 55.613218908623786 17.198051687377898 13.836008100127566 13.352721303870755 2 14.612390564880387 25.722857302180337 39.25009758545698 20.414654547482293 3 24.28836198917405 34.923845867336354 30.384261107530214 10.403531035959382 4 10.705906491699153 18.58412803197231 36.906206232102555 33.803759244225986 5 9.971720920274405 44.83661471761064 30.014853207489946 15.17681115462501 6 21.096684121787384 38.40959169290882 18.55335111555171 21.940373069752088 7 21.36430948196651 45.13657814214475 16.824268267661044 16.674844108227695 8 23.57929011898257 30.113853050391963 19.580048395909767 26.726808434715704 9 28.53673277771211 22.10791124631178 20.089788508587446 29.265567467388664 10-11 22.680245680080642 28.740398940203576 22.716375103704827 25.862980276010955 12-13 33.86007208043034 28.278856704461315 23.013885295770628 14.847185919337717 14-15 28.584172636199007 27.215826471716458 31.198872405149114 13.001128486935423 16-17 15.46357379579202 32.64218775786934 32.776670101207436 19.117568345131204 18-19 14.183809557793698 32.76191601916197 38.21143741580953 14.842837007234804 20-21 17.684349268936725 29.58208069796694 34.9730590470753 17.760510986021036 22-23 16.037338198167657 29.46153777531959 33.53245403534439 20.968669991168362 24-25 17.29985190736181 29.948056061092675 36.912759162116835 15.839332869428683 26-27 20.133835961722916 26.5105618879793 38.64758760678995 14.708014543507842 28-29 15.503648625743777 27.573886901522787 41.8171315913896 15.105332881343836 30-31 14.423222744587278 34.65436184732865 38.185901496025764 12.736513912058307 32-33 13.57032381661803 33.94894806154958 35.737472024692984 16.74325609713941 34-35 15.15277238721075 30.015569444630323 36.36848280446294 18.46317536369598 36-37 18.32543813869671 30.369401437193055 37.63777560697096 13.667384817139263 38-39 20.825549635918133 26.651528653114575 39.16102244046935 13.361899270497938 40-41 14.896863066985475 29.943502194782486 41.769454159380466 13.390180578851577 42-43 13.110898482455905 34.36789069400209 38.135767113632916 14.38544370990909 44-45 13.752078317585884 35.49627045839517 34.27598719388375 16.47566403013519 46-47 13.688033578061857 32.66367520986289 31.52771706646922 22.120574145606035 48-49 16.16122643781725 31.575109057334274 33.41603700366647 18.847627501182014 50-51 19.825129129236284 33.02798023140673 33.29928544028832 13.847605199068663 52-53 14.633308652327894 32.915020027297786 37.13435685165526 15.317314468719056 54-55 11.84086103999215 32.486261898177474 37.71911826364666 17.953758798183717 56-57 15.603227561843761 36.60769244493608 30.728409324067545 17.060670669152607 58-59 13.809022542976173 33.32549042347252 32.87576830780485 19.989718725746453 60-61 14.893908846802322 30.423427924030083 34.45163563699296 20.231027592174637 62-63 21.54350696271979 29.824727691196017 32.2897802795793 16.3419850665049 64-65 19.09027449441109 30.94329018617804 35.540982363488766 14.425452955922102 66-67 14.42701410385648 32.20135240015344 36.712846909373134 16.658786586616948 68-69 13.857702961287819 36.35584072651689 34.48223182704188 15.304224485153417 70-71 14.504179416041463 35.02669562967787 30.699862618981776 19.769262335298894 72-73 14.042748690865947 32.96207748646262 29.80365219408191 23.191521628589527 74-75 15.695112268838596 33.02240470306966 32.28320115614156 18.999281871950185 76-77 17.994794686744516 29.73161636796703 34.50906357886473 17.764525366423722 78-79 14.588592915064632 29.697940176811155 37.45740296350482 18.25606394461939 80-81 13.525116640052811 35.39869488032686 34.29797407602344 16.778214403596888 82-83 13.322962839062194 34.248947617852856 31.973794987594435 20.454294555490506 84-85 14.666442149963032 30.43398261036353 32.23940914935305 22.660166090320395 86-87 16.74868277996153 30.622954475760363 33.840818488472586 18.787544255805525 88-89 19.05082099746313 27.352903459619192 35.06353321624711 18.532742326670572 90-91 14.604571497389532 29.543898462711898 37.619400205625944 18.232129834272627 92-93 12.69267782450107 33.213124004063474 35.08607010278009 19.00812806865536 94-95 12.935337252558051 34.15602112456176 32.306841396290714 20.60180022658947 96-97 12.836092848158293 31.383868435372936 30.33210076986895 25.44793794659982 98-99 14.949775640739716 30.222708903895736 32.02806497943745 22.7994504759271 100-101 18.089801689608105 30.790855241442678 32.59654584868462 18.522797220264593 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 11707.0 1 7089.5 2 3890.5 3 5009.0 4 3599.0 5 3189.5 6 6282.0 7 12135.0 8 16656.0 9 16459.0 10 10948.5 11 5728.0 12 4697.5 13 4517.0 14 4058.0 15 3373.5 16 2699.5 17 2242.0 18 1996.0 19 1862.0 20 1821.0 21 1757.5 22 1791.5 23 1898.0 24 1985.0 25 2132.5 26 2333.5 27 2570.0 28 2738.0 29 2943.0 30 3192.5 31 3363.0 32 3592.0 33 3850.5 34 4098.0 35 4375.5 36 4760.0 37 5074.0 38 5279.0 39 5323.0 40 5167.5 41 4891.5 42 4742.5 43 4941.0 44 5526.0 45 5911.0 46 7900.0 47 14362.0 48 64228.5 49 67453.5 50 22158.0 51 13782.0 52 6273.0 53 3842.5 54 3426.5 55 3097.5 56 2980.5 57 2893.0 58 2703.0 59 2656.0 60 2640.5 61 2592.0 62 2231.0 63 1316.0 64 645.5 65 523.5 66 465.5 67 358.5 68 281.5 69 230.5 70 186.0 71 152.0 72 132.0 73 169.5 74 160.0 75 82.5 76 44.5 77 34.0 78 26.5 79 12.0 80 3.5 81 1.0 82 0.5 83 0.5 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014050331409404355 3 0.004683443803134785 4 0.0 5 0.0 6 0.0 7 0.0 8 6.690634004478265E-4 9 2.2302113348260881E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.4604226696521763E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.004460422669652177 26-27 0.017395648411643486 28-29 0.0 30-31 0.0 32-33 3.3453170022391325E-4 34-35 0.01650356387771305 36-37 0.0031222958687565235 38-39 0.015276947643558702 40-41 0.011597098941095658 42-43 0.007136676271443482 44-45 0.0018956796346021748 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 4.4604226696521763E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.1151056674130441E-4 84-85 0.003902869835945654 86-87 1.1151056674130441E-4 88-89 1.1151056674130441E-4 90-91 2.2302113348260881E-4 92-93 7.805739671891309E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 448388.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.81649389194542 #Duplication Level Percentage of deduplicated Percentage of total 1 76.67186689175944 19.793987832951597 2 11.799990217454013 6.092687507478345 3 4.390190402893421 3.4001797116232626 4 2.1390985711237276 2.208961007827395 5 1.1807040866369678 1.5240819920429136 6 0.7968321378641533 1.2342847212045436 7 0.5081639729475038 0.9183308472594165 8 0.36815824456911284 0.760364405775028 9 0.23938912786796077 0.5562169161661216 >10 1.5763383123196073 7.653392045105753 >50 0.1538312602425447 2.6407471091949763 >100 0.12790464334773377 6.552783254229171 >500 0.015555970136886543 2.850471871162134 >1k 0.022469734642169448 14.39336579151625 >5k 0.003456882252641454 6.079786982558559 >10k+ 0.006049543942122544 23.340358003904534 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 20216 4.50859523448442 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17911 3.9945315218070063 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 16614 3.705273111680063 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14766 3.293130057004202 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 12612 2.8127425354826623 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11428 2.5486855134392536 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11066 2.467951863118549 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 8005 1.7852841735282836 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 6908 1.5406299900978617 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 6683 1.4904502350642748 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 5654 1.2609614887106702 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4793 1.068940292782144 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4355 0.9712570363167614 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 4331 0.9659045291131787 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4078 0.9094801823420787 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 3874 0.8639838711116266 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3609 0.8048832707387352 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3374 0.7524733043703221 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3068 0.6842288375246438 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3048 0.6797684148549916 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2986 0.6659411045790699 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2766 0.616876455212896 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2711 0.6046102928713525 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2682 0.5981426800003569 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 2004 0.44693435149914806 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1883 0.41994879434775234 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1819 0.40567544180486537 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1637 0.3650855955110306 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1596 0.35594172903824367 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1542 0.3438985878301828 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1419 0.3164669884118219 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1287 0.28702819879211755 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1236 0.2756541209845045 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1172 0.2613807684416175 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1117 0.24911460610007405 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1069 0.23840959169290885 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1056 0.23551031695763489 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 997 0.222352070082161 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 976 0.2176686262790262 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 956 0.21320820360937404 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 928 0.20696361187186096 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 811 0.18087013925439574 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 794 0.1770787799851914 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 735 0.16392053310971746 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 718 0.16012917384051312 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 682 0.15210041303513921 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 653 0.14563280016414354 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 646 0.14407165222976528 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 629 0.14028029296056094 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 616 0.137381018225287 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 557 0.1242227713498131 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 529 0.11797817961230006 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 526 0.11730911621185224 TruSeq Adapter, Index 11 (95% over 21bp) GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 512 0.11418682034309571 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 511 0.1139637992096131 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 499 0.11128754560782178 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 494 0.11017243994040876 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 470 0.10481993273682615 No Hit TTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCG 461 0.10281274253548267 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 454 0.10125159460110442 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.2302113348260881E-4 2.2302113348260881E-4 14-15 0.0 0.0 0.0 2.2302113348260881E-4 2.2302113348260881E-4 16-17 0.0 0.0 0.0 5.57552833706522E-4 2.2302113348260881E-4 18-19 0.0 0.0 0.0 0.001338126800895653 2.2302113348260881E-4 20-21 0.0 0.0 0.0 0.0023417219015673925 2.2302113348260881E-4 22-23 0.0 0.0 0.0 0.007359697404926091 2.2302113348260881E-4 24-25 0.0 0.0 0.0 0.017395648411643486 2.2302113348260881E-4 26-27 0.0 0.0 0.0 0.036352444757665234 2.2302113348260881E-4 28-29 0.0 0.0 0.0 0.07214733668162394 2.2302113348260881E-4 30-31 0.0 0.0 0.0 0.15176588133491528 2.2302113348260881E-4 32-33 0.0 0.0 0.0 0.2595965993737567 2.2302113348260881E-4 34-35 0.0 0.0 0.0 0.4333300623567089 2.2302113348260881E-4 36-37 0.0 0.0 0.0 0.6683943370473786 2.2302113348260881E-4 38-39 0.0 0.0 0.0 0.9208542601496917 2.2302113348260881E-4 40-41 0.0 0.0 0.0 1.1969544234011615 2.2302113348260881E-4 42-43 0.0 0.0 0.0 1.442946733632479 2.2302113348260881E-4 44-45 0.0 0.0 0.0 1.697190825802653 2.2302113348260881E-4 46-47 0.0 0.0 0.0 2.0170031312167143 2.2302113348260881E-4 48-49 0.0 0.0 0.0 2.383984406362347 2.2302113348260881E-4 50-51 0.0 0.0 0.0 2.706361454811458 2.2302113348260881E-4 52-53 0.0 0.0 0.0 2.9591559096139948 2.2302113348260881E-4 54-55 0.0 0.0 0.0 3.1986806069743166 2.2302113348260881E-4 56-57 0.0 0.0 0.0 3.436086603566554 2.2302113348260881E-4 58-59 0.0 0.0 0.0 3.818902379189452 2.2302113348260881E-4 60-61 0.0 0.0 0.0 4.3992033685112 2.2302113348260881E-4 62-63 0.0 0.0 0.0 4.8252852440297245 2.2302113348260881E-4 64-65 0.0 0.0 0.0 5.096590452911318 2.2302113348260881E-4 66-67 0.0 0.0 0.0 5.392427986475998 2.2302113348260881E-4 68-69 0.0 0.0 0.0 5.7190424364612795 2.2302113348260881E-4 70-71 0.0 0.0 0.0 6.108660356655397 2.2302113348260881E-4 72-73 0.0 0.0 0.0 6.568195402196313 2.2302113348260881E-4 74-75 0.0 0.0 0.0 6.995949936215956 2.2302113348260881E-4 76-77 0.0 0.0 0.0 7.323567981301908 2.2302113348260881E-4 78-79 0.0 0.0 0.0 7.666909016298384 2.2302113348260881E-4 80-81 0.0 0.0 0.0 8.021401107968991 2.2302113348260881E-4 82-83 0.0 0.0 0.0 8.388605404248107 2.2302113348260881E-4 84-85 0.0 0.0 0.0 9.032244395478916 2.2302113348260881E-4 86-87 0.0 0.0 0.0 9.719372507738832 2.2302113348260881E-4 88-89 0.0 0.0 0.0 10.130400456747282 2.2302113348260881E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCATACT 15 6.615271E-4 95.0 1 GCTTATG 75 0.0 63.333336 1 CATACTC 25 0.005019284 57.0 2 TTAGGAG 35 2.7861053E-4 54.28571 3 CGTCACT 70 1.2914825E-9 54.28571 1 CTTATGT 95 7.2759576E-12 50.0 2 ATAACGC 50 2.8242144E-5 47.5 3 AGATAGT 40 5.3846434E-4 47.499996 6 TAACGCA 75 1.360313E-7 44.333336 4 GTATATG 60 8.282658E-5 39.583336 1 TATGTAC 120 8.731149E-11 39.583336 4 TATAACG 60 8.282658E-5 39.583336 2 TAGTCAA 50 0.0016147647 38.0 9 GTCACTA 115 2.4956535E-9 37.173912 2 TCACTAC 120 3.783498E-9 35.625 3 TTATGTA 150 2.5465852E-11 34.833336 3 GTACCCT 55 0.0025779544 34.545456 6 GATAGTC 55 0.0025779544 34.545456 7 CGAGTGC 35 2.0529737E-4 33.92857 82-83 ACTACCT 130 8.263669E-9 32.884617 5 >>END_MODULE