##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938008_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 444214 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.103328575866584 33.0 31.0 34.0 31.0 34.0 2 32.07215216089543 33.0 31.0 34.0 30.0 34.0 3 32.0243306154241 34.0 31.0 34.0 30.0 34.0 4 35.54785531297978 37.0 35.0 37.0 35.0 37.0 5 35.62222262242973 37.0 35.0 37.0 35.0 37.0 6 35.7734065112761 37.0 36.0 37.0 35.0 37.0 7 35.76781911421072 37.0 36.0 37.0 35.0 37.0 8 35.802896801991835 37.0 37.0 37.0 35.0 37.0 9 37.352933496017684 39.0 37.0 39.0 35.0 39.0 10-11 37.312887482159496 39.0 37.5 39.0 35.0 39.0 12-13 37.15406538290103 39.0 37.0 39.0 34.5 39.0 14-15 37.975410500344424 40.0 38.0 41.0 33.0 41.0 16-17 37.90638070839731 40.0 37.5 41.0 33.5 41.0 18-19 38.38021989401505 40.0 38.0 41.0 34.0 41.0 20-21 38.439907341956804 40.0 38.0 41.0 35.0 41.0 22-23 38.31177990788224 40.0 38.0 41.0 34.5 41.0 24-25 38.133800375494694 40.0 38.0 41.0 34.0 41.0 26-27 37.69381424268483 40.0 36.5 41.0 33.5 41.0 28-29 37.39857591161017 39.5 35.5 41.0 33.0 41.0 30-31 37.009174632046715 39.0 35.0 40.5 33.0 41.0 32-33 36.48287649646342 38.0 35.0 40.0 33.0 41.0 34-35 36.17249118668029 38.0 35.0 40.0 33.0 41.0 36-37 35.73265588207485 37.0 35.0 40.0 31.0 41.0 38-39 35.20991684188252 36.0 35.0 40.0 30.5 41.0 40-41 34.817733119622524 36.0 35.0 40.0 29.5 41.0 42-43 34.62381194649426 36.0 35.0 40.0 27.5 41.0 44-45 34.121108294650774 36.0 35.0 40.0 23.5 41.0 46-47 33.37928791078174 35.0 33.5 40.0 20.5 41.0 48-49 32.765422746694156 35.0 33.0 40.0 11.5 41.0 50-51 31.942088723002875 35.0 32.0 39.0 6.0 40.5 52-53 31.805457504716195 35.0 32.5 39.0 2.0 40.0 54-55 31.959151445024247 35.0 33.0 39.0 2.0 41.0 56-57 31.852414601971123 35.0 33.0 39.0 2.0 41.0 58-59 31.55702431710842 35.0 32.5 39.0 2.0 41.0 60-61 31.041152462551835 35.0 31.5 38.5 2.0 40.5 62-63 30.452454222514376 35.0 31.0 38.0 2.0 40.0 64-65 29.73868563350097 35.0 29.0 37.0 2.0 40.0 66-67 29.363892178094343 35.0 29.0 36.5 2.0 39.0 68-69 28.92908147874673 35.0 29.0 36.0 2.0 39.0 70-71 28.32751444123778 35.0 27.0 35.5 2.0 38.0 72-73 27.544450422544088 34.0 26.0 35.0 2.0 37.0 74-75 26.970776472601045 33.5 25.5 35.0 2.0 37.0 76-77 26.179924540874442 33.0 22.0 35.0 2.0 36.0 78-79 25.52219425772263 33.0 19.0 35.0 2.0 35.0 80-81 25.051695804274516 32.5 15.5 35.0 2.0 35.0 82-83 24.457870530870256 32.0 4.5 35.0 2.0 35.0 84-85 23.806629237259518 32.0 2.0 35.0 2.0 35.0 86-87 22.98434988541559 31.0 2.0 35.0 2.0 35.0 88-89 22.007673103504167 30.0 2.0 34.5 2.0 35.0 90-91 21.10915009432391 29.0 2.0 34.0 2.0 35.0 92-93 20.4512937458072 29.0 2.0 34.0 2.0 35.0 94-95 19.603756297640327 27.5 2.0 34.0 2.0 35.0 96-97 18.380128721742224 24.5 2.0 33.5 2.0 35.0 98-99 17.098404147550504 11.0 2.0 33.0 2.0 35.0 100-101 13.829949078597252 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1350119454728258 1101 2 0.19664079589541927 1101 3 0.08670545297683319 1101 4 0.14057250977449343 1101 5 0.03593635184000732 1101 6 -0.12248197992549592 1101 7 -0.2137572268986787 1101 8 -0.3450497471542988 1101 9 -0.1015980533586216 1101 10-11 0.038802111868371014 1101 12-13 0.3087335989309281 1101 14-15 -0.022995661213023766 1101 16-17 0.03897576412752812 1101 18-19 0.16170120141324418 1101 20-21 -0.04114075528633521 1101 22-23 -0.10140274441138075 1101 24-25 -0.10440893129358386 1101 26-27 -3.694500929398714E-4 1101 28-29 0.07951340009575603 1101 30-31 -0.036872270705750054 1101 32-33 0.013479323281885058 1101 34-35 0.15463729368318013 1101 36-37 0.4661416967704852 1101 38-39 0.10261036735085582 1101 40-41 0.5089276265999061 1101 42-43 0.6234721512732975 1101 44-45 0.7484027089505432 1101 46-47 0.8008320316105184 1101 48-49 0.653207000238865 1101 50-51 1.0896107225829361 1101 52-53 1.1756506117728804 1101 54-55 0.882376043037489 1101 56-57 0.7543189133165704 1101 58-59 0.8230691339337959 1101 60-61 0.9054557721921093 1101 62-63 0.6922053281245546 1101 64-65 0.311563341663053 1101 66-67 0.3883936356059543 1101 68-69 0.7612317935899569 1101 70-71 0.46382076908149017 1101 72-73 0.38343348546930045 1101 74-75 0.29215711692943813 1101 76-77 -0.04625468463004978 1101 78-79 0.24519233517935035 1101 80-81 0.16936291283768412 1101 82-83 0.15655966975044677 1101 84-85 0.5694178457818602 1101 86-87 0.49282359010479126 1101 88-89 0.7063801198006239 1101 90-91 0.6739454767012312 1101 92-93 0.296576091757224 1101 94-95 0.5056638984567634 1101 96-97 0.23847821974520755 1101 98-99 -0.21543145679390818 1101 100-101 -0.26956357760086114 1102 1 0.08352082378858938 1102 2 0.14959038696423477 1102 3 0.11958165411499877 1102 4 0.2953879465378719 1102 5 0.23786439239105306 1102 6 0.08962113914655845 1102 7 0.25450784757966716 1102 8 0.261107543637749 1102 9 0.44676155730157774 1102 10-11 0.326972650164862 1102 12-13 -0.008764923941235736 1102 14-15 0.10906267756235621 1102 16-17 0.16984929088685874 1102 18-19 0.3165850525187537 1102 20-21 0.49927035271503684 1102 22-23 0.6526165981889775 1102 24-25 0.3890201821187347 1102 26-27 0.36009638938212873 1102 28-29 0.21744604306618243 1102 30-31 0.3143420838671247 1102 32-33 0.17130499109915576 1102 34-35 0.20088227922287416 1102 36-37 0.4701408260424884 1102 38-39 0.13234261849368778 1102 40-41 0.5533751807871781 1102 42-43 0.5633639322230124 1102 44-45 0.9413871291389881 1102 46-47 0.6600881811269517 1102 48-49 0.830994107245111 1102 50-51 0.20249438710388645 1102 52-53 0.2335011575327428 1102 54-55 0.18511261672284718 1102 56-57 0.379066246712096 1102 58-59 0.20379805765179881 1102 60-61 0.2502982637180615 1102 62-63 0.3808920837298686 1102 64-65 0.529400826503057 1102 66-67 -0.011379353167875195 1102 68-69 -0.19650680142823518 1102 70-71 -0.9298135873572342 1102 72-73 -0.6295590200504897 1102 74-75 -0.2996456009857873 1102 76-77 -0.9465812076265969 1102 78-79 -1.2071290878389682 1102 80-81 -0.6417202105880087 1102 82-83 -0.9791961532429916 1102 84-85 -0.9770386022698823 1102 86-87 -0.6536670651180216 1102 88-89 -1.1326658111008925 1102 90-91 -1.321113141951006 1102 92-93 -1.0673695499382099 1102 94-95 -1.2252935913588274 1102 96-97 -1.2910954345135863 1102 98-99 -1.2513645351283298 1102 100-101 -1.7176464211497002 1103 1 -0.16528400941340848 1103 2 -0.003548567593156804 1103 3 -0.06276665163756334 1103 4 0.01711108811917228 1103 5 -0.10109016500322099 1103 6 -0.30113273529526197 1103 7 -0.15263688580620283 1103 8 -0.1450390284662717 1103 9 -0.13285493862697706 1103 10-11 -0.22968613628116685 1103 12-13 -0.34293881414349414 1103 14-15 -0.3206982890780452 1103 16-17 -0.018619319567562798 1103 18-19 -0.15244568034176353 1103 20-21 -0.10304117867450913 1103 22-23 -0.19069834796284368 1103 24-25 -0.16593122318727893 1103 26-27 -0.2885771068090932 1103 28-29 0.01847047175018446 1103 30-31 -0.33406873830256245 1103 32-33 -0.5013243758564556 1103 34-35 -0.5203155143153495 1103 36-37 -0.5917451734321659 1103 38-39 -0.504930825162738 1103 40-41 -0.19466789775236037 1103 42-43 -0.147195563442871 1103 44-45 -0.6126711844937915 1103 46-47 -0.7946268175596813 1103 48-49 -0.8653809912576449 1103 50-51 -0.5331242102168439 1103 52-53 -0.3946312301741095 1103 54-55 -0.6221109188694882 1103 56-57 -0.5888875226601122 1103 58-59 -0.6644971879484096 1103 60-61 -0.7209651983255441 1103 62-63 -0.8152203407513525 1103 64-65 -1.2806858559240517 1103 66-67 -0.8133231409272668 1103 68-69 -0.8478070378348548 1103 70-71 -1.0952719535248185 1103 72-73 -1.4046312554064144 1103 74-75 -1.3123590236282894 1103 76-77 -1.1108050058929457 1103 78-79 -1.2400158753356862 1103 80-81 -1.1570866408480533 1103 82-83 -1.1562384437604578 1103 84-85 -1.4052235247810465 1103 86-87 -1.5046783601009466 1103 88-89 -1.5676014559656117 1103 90-91 -1.5308320807479596 1103 92-93 -1.589460435149661 1103 94-95 -1.80254602109353 1103 96-97 -1.45815611221256 1103 98-99 -1.4339229800981226 1103 100-101 -1.3169372012915428 1104 1 0.06918300111743036 1104 2 0.02607748011799771 1104 3 0.1874267719601157 1104 4 -0.07189600407940588 1104 5 0.18581613200946379 1104 6 0.09932933218808415 1104 7 0.021707623112774854 1104 8 0.009844917375126272 1104 9 0.15456178176846436 1104 10-11 0.12629924949145988 1104 12-13 -0.018038212381185303 1104 14-15 -0.013678624345608625 1104 16-17 -0.05032467095377058 1104 18-19 -0.03366747273376092 1104 20-21 0.05126249637384461 1104 22-23 0.007190111095830787 1104 24-25 0.1424853897506111 1104 26-27 0.11639549151456663 1104 28-29 -0.01719200407186605 1104 30-31 -0.12028193409022236 1104 32-33 0.13181284744034372 1104 34-35 0.18556241390300698 1104 36-37 0.4129157979841338 1104 38-39 0.03848739963846981 1104 40-41 0.30113051187584716 1104 42-43 0.33276628912536665 1104 44-45 0.38073898099084147 1104 46-47 0.3609018736073111 1104 48-49 0.6309520197030309 1104 50-51 0.6179826025920967 1104 52-53 0.494107218138808 1104 54-55 0.22546054040410013 1104 56-57 0.3685163028288194 1104 58-59 0.20656180624888165 1104 60-61 0.43015236023882153 1104 62-63 0.10356323973435622 1104 64-65 -0.0035958064935783796 1104 66-67 0.07374971529452523 1104 68-69 -0.0038300337514698413 1104 70-71 0.35994511073479885 1104 72-73 0.5495515298933924 1104 74-75 0.5344172497437292 1104 76-77 0.5792605432151525 1104 78-79 0.4680112039679898 1104 80-81 0.1560070621392633 1104 82-83 -0.016078869292371678 1104 84-85 -0.02221256411051442 1104 86-87 -0.3300840123683031 1104 88-89 -0.31009285936127284 1104 90-91 -0.4599880016591982 1104 92-93 -0.6111265645285577 1104 94-95 -0.8047408416394113 1104 96-97 -0.8473243907425427 1104 98-99 -0.7845435474740086 1104 100-101 -0.8094393504426289 1105 1 0.10374011067454347 1105 2 0.1378490418895595 1105 3 -0.0035988690655273103 1105 4 0.0025795203961180846 1105 5 -0.00806498687165913 1105 6 0.14466732752607925 1105 7 0.12561205201720327 1105 8 0.04883093136113814 1105 9 0.1043286815353639 1105 10-11 0.22023864343085364 1105 12-13 0.21701423517126273 1105 14-15 0.16100086283388038 1105 16-17 0.1299200842909869 1105 18-19 0.23492227085598216 1105 20-21 0.22567974579109062 1105 22-23 0.16668894559466452 1105 24-25 -0.03481647505125096 1105 26-27 0.2151425777616538 1105 28-29 0.024620350240489586 1105 30-31 0.008505944697652978 1105 32-33 0.06634081946832282 1105 34-35 -0.16373828539769164 1105 36-37 -0.10733478476645786 1105 38-39 0.10066688681795455 1105 40-41 -0.20784384709851622 1105 42-43 -0.04173837287929416 1105 44-45 -0.2815861938343289 1105 46-47 -0.20267621497077926 1105 48-49 -0.15911208286107836 1105 50-51 0.08115276576225838 1105 52-53 -0.09307226904068244 1105 54-55 0.05121811616167804 1105 56-57 -0.2431387088261907 1105 58-59 -0.3289568417697666 1105 60-61 -0.5056285255420612 1105 62-63 -0.3831500469789333 1105 64-65 -0.4299361328339053 1105 66-67 -0.11191462036980937 1105 68-69 -0.45680206172349713 1105 70-71 -0.4608590850693979 1105 72-73 -0.6306640878222254 1105 74-75 -0.8167482514217745 1105 76-77 -0.32254598359137177 1105 78-79 -0.56194217598539 1105 80-81 -0.7139812828490797 1105 82-83 -0.6742665149800189 1105 84-85 -0.9241939160918626 1105 86-87 -0.8027821475664396 1105 88-89 -0.5587816705500863 1105 90-91 -0.22814068231188145 1105 92-93 -0.7549202708222644 1105 94-95 -1.0972524966510697 1105 96-97 -1.1948768382949915 1105 98-99 -1.3208197712502319 1105 100-101 -1.2052144087176888 1106 1 0.09749963199311651 1106 2 0.03420203273073952 1106 3 0.1034253403208858 1106 4 -0.10719784134975185 1106 5 -0.0862728682291447 1106 6 -0.16658572388445236 1106 7 0.011191039958397653 1106 8 -0.10879621839199416 1106 9 0.06330671137953914 1106 10-11 -0.0685945705992097 1106 12-13 0.14383544545703586 1106 14-15 0.4849643009705389 1106 16-17 -0.24582097255242985 1106 18-19 0.20118697727816226 1106 20-21 0.47399573426456243 1106 22-23 0.47666636974530974 1106 24-25 0.29932444964085647 1106 26-27 0.4471966129652927 1106 28-29 0.36238625887254017 1106 30-31 0.48154340592409284 1106 32-33 0.5204641239853203 1106 34-35 0.5576335186512793 1106 36-37 0.5705005033050554 1106 38-39 0.7992425893306674 1106 40-41 0.7451414877765856 1106 42-43 0.7237022232699672 1106 44-45 0.8092494796785132 1106 46-47 1.1053005851320066 1106 48-49 0.6321897911179661 1106 50-51 0.42967432294523533 1106 52-53 0.5605352782676505 1106 54-55 0.25844789425759984 1106 56-57 -0.39671872461093116 1106 58-59 0.06351420424458709 1106 60-61 0.47547567209756636 1106 62-63 0.1913227243442357 1106 64-65 0.11222380696383283 1106 66-67 0.3603519581959844 1106 68-69 0.2776839247309937 1106 70-71 0.4332271436625028 1106 72-73 0.28244642371348405 1106 74-75 0.197302002785964 1106 76-77 -0.2240381921701946 1106 78-79 0.0782116334597589 1106 80-81 0.027571127994665545 1106 82-83 -0.004956822048278298 1106 84-85 -0.32525479746770714 1106 86-87 -0.39258997753174896 1106 88-89 -0.5334434916685993 1106 90-91 -0.4467960426995248 1106 92-93 -0.5372927539821468 1106 94-95 -0.9022742465547076 1106 96-97 -0.398123722644236 1106 98-99 -0.6652850172902802 1106 100-101 -1.4862378472214406 1107 1 0.016864173900025037 1107 2 -0.05217256598194808 1107 3 0.045687882046781425 1107 4 0.13530480727961702 1107 5 0.10565293820529575 1107 6 0.05768264216411012 1107 7 0.27664492718800915 1107 8 0.15581117221561414 1107 9 0.005963220086741217 1107 10-11 0.37959078554161607 1107 12-13 0.21939724769625712 1107 14-15 0.15814739446316395 1107 16-17 0.1404876100714958 1107 18-19 0.378203263021355 1107 20-21 0.4444535345445999 1107 22-23 0.38649584592894826 1107 24-25 0.2963324301341643 1107 26-27 0.38272178692301395 1107 28-29 0.4656242415486318 1107 30-31 0.2844294802560796 1107 32-33 0.3076633914196947 1107 34-35 -0.0598644715847243 1107 36-37 -0.046158976126143614 1107 38-39 -0.013010848563631328 1107 40-41 0.14688931696527874 1107 42-43 0.07149254765385393 1107 44-45 0.03218421425656004 1107 46-47 0.047251679987546424 1107 48-49 0.41496225389074226 1107 50-51 0.40018802394559216 1107 52-53 0.49644909532855763 1107 54-55 0.17405910208582753 1107 56-57 0.1493940457755336 1107 58-59 -0.11944086754791172 1107 60-61 0.3637407799327157 1107 62-63 0.3616122900754952 1107 64-65 0.17097546402089847 1107 66-67 0.19489114992480694 1107 68-69 0.4932610597392184 1107 70-71 0.29127924313383957 1107 72-73 0.5809110172620073 1107 74-75 0.4246901614162333 1107 76-77 0.3278083949577315 1107 78-79 -0.09006550633995758 1107 80-81 -0.21271412539531198 1107 82-83 -0.3774632506311164 1107 84-85 -0.028509816553810907 1107 86-87 0.0487169280705686 1107 88-89 -0.11869741403580747 1107 90-91 -0.4233869505804613 1107 92-93 -0.42913550791782384 1107 94-95 -0.486127988974836 1107 96-97 -0.7427143962745362 1107 98-99 -0.6873072391575796 1107 100-101 -0.6887981002121304 1108 1 0.10368290816334991 1108 2 0.05423327115722287 1108 3 0.08490771637357497 1108 4 0.11000583641138917 1108 5 0.16063951925616493 1108 6 0.10731408771885498 1108 7 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90-91 -0.8572725945763224 2316 92-93 -0.6778708142547352 2316 94-95 -0.36703091100779517 2316 96-97 -0.7541699548170193 2316 98-99 -0.8781680454659764 2316 100-101 -0.600845885674465 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2466.0 3 446.0 4 324.0 5 307.0 6 345.0 7 457.0 8 542.0 9 719.0 10 915.0 11 1385.0 12 2258.0 13 3371.0 14 4067.0 15 5502.0 16 7243.0 17 9922.0 18 8978.0 19 5424.0 20 4997.0 21 5269.0 22 5821.0 23 6554.0 24 7160.0 25 8008.0 26 9092.0 27 10418.0 28 11910.0 29 14141.0 30 16599.0 31 19092.0 32 23374.0 33 29335.0 34 42831.0 35 78522.0 36 47986.0 37 34419.0 38 13314.0 39 701.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 56.59065189653696 16.784566206339974 13.743975401151493 12.880806495971578 2 14.512205559734943 25.75442927352256 39.819315371482375 19.914049795260127 3 24.129511390076296 35.60796674061649 29.82725501866782 10.435266850639396 4 10.631473362711178 18.22177642033608 37.37177247574074 33.774977741212005 5 10.07977841584471 44.77538214470185 29.559966888686894 15.584872550766546 6 20.73505495769622 38.783464646851975 18.73501435689458 21.746466038557227 7 21.695345601648803 45.29610596360996 17.054702315586294 15.953846119154944 8 23.430382655474908 29.76427017017423 19.5702616366823 27.235085537668564 9 29.20783040876357 22.215460211200146 20.121716198397404 28.454993181638887 10-11 22.746493869976575 28.828405540400915 22.80305616681145 25.622044422811058 12-13 34.24346514461965 28.352597539487583 22.87072558398559 14.533211731907183 14-15 28.648687032864473 27.247294544905316 31.5081679326502 12.59585048958001 16-17 15.356229909516747 32.495300010369284 32.83106185952779 19.31740822058618 18-19 14.011160238803413 32.9564038378959 38.492229421689544 14.540206501611145 20-21 17.919188503803888 29.295914342068187 35.33231896308819 17.45257819103973 22-23 16.129014067351406 29.427302142978785 33.7661546720682 20.677529117601615 24-25 17.244767203143965 29.764225221201006 37.2133185339596 15.777689041695432 26-27 20.21372531337361 26.195328704121202 38.93779871945171 14.653147263053476 28-29 15.625158488741523 27.658356315374842 41.810034408257984 14.90645078762565 30-31 14.367592050114922 34.759114876740725 38.14333678849881 12.729956284645544 32-33 13.735102696306315 33.59851238272336 35.93350801566506 16.73287690530527 34-35 15.502635786077146 29.79221285479461 36.37734046424536 18.32781089488289 36-37 18.36906438348448 30.09846223173548 37.979947051202615 13.552526333577426 38-39 20.965258922327244 26.570071702953594 39.27017722465537 13.194492150063796 40-41 14.815907667773368 30.01135232294656 41.959672224199366 13.213067785080703 42-43 13.098626289156165 34.28544398104714 37.99627655321004 14.619653176586661 44-45 13.792575376771252 35.41672296840797 34.39000441405652 16.400697240764263 46-47 13.83129555991752 32.456285565311795 31.78532375202567 21.92709512274502 48-49 16.323628108517145 31.775902995797505 33.454310822436724 18.446158073248622 50-51 20.104758268774443 33.09976136016821 33.10268945626382 13.692790914793529 52-53 14.728578312384883 33.041323467820746 37.09486023606976 15.135237983724606 54-55 12.07810694601796 32.42210815327683 37.76377817969688 17.736006721008334 56-57 15.707202399448775 36.395580294617005 31.053676858019102 16.843540447915117 58-59 14.090954648804422 32.933362312932374 32.99777810561262 19.977904932650585 60-61 15.226577938419444 29.955354378142225 34.871327151631846 19.946740531806487 62-63 22.20009977623546 29.06833095529552 32.59101766129459 16.14055160717443 64-65 19.491830782916146 30.390388361539934 36.03249670641489 14.085284149129029 66-67 14.949825696089647 31.597425526740114 37.18303261780152 16.269716159368723 68-69 14.308675153123401 36.112456439517665 34.802657217580524 14.776211189778406 70-71 15.181563868317207 34.56189073283241 31.175896895327675 19.080648503522713 72-73 14.794092903984351 32.81990294487634 30.182701934457945 22.203302216681365 74-75 16.38174600940289 32.81957152107204 32.831620731398324 17.96706173812674 76-77 18.89986816611295 29.492625295669296 34.566935250535046 17.040571287682706 78-79 15.476127483706525 29.93732542705285 37.23783801431822 17.348709074922404 80-81 14.510305477152544 35.608528096174695 34.1364779102404 15.744688516432358 82-83 14.591568260344346 34.06605654559189 31.877568987626077 19.464806206437682 84-85 16.154954630401008 30.382883389998423 32.241348253889626 21.22081372571094 86-87 18.515199380338583 30.382436021422453 33.7229109225457 17.379453675693263 88-89 20.808192531703597 27.223588219856666 34.854415188441976 17.11380405999777 90-91 16.44202998986966 29.656999457987247 37.06319237621319 16.837778175929902 92-93 15.074230573826725 33.33723852577483 34.38071345612911 17.207817444269335 94-95 15.473580812097426 34.33964984691222 31.529999121350507 18.656770219639842 96-97 15.853356341207373 31.147360026672317 29.579573152874705 23.419710479245605 98-99 18.027484396151912 30.20805881597708 30.925351551149767 20.839105236721245 100-101 21.31451673803102 29.83112627940187 31.806260150964068 17.048096831603043 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 11858.0 1 7251.0 2 4025.0 3 4988.0 4 3531.0 5 3114.5 6 5871.0 7 11434.0 8 16348.5 9 16247.5 10 10688.0 11 5713.5 12 4727.5 13 4549.5 14 4066.5 15 3408.0 16 2766.0 17 2341.0 18 2100.5 19 1958.0 20 1860.5 21 1858.0 22 1882.0 23 1948.5 24 2117.0 25 2256.5 26 2469.5 27 2698.5 28 2866.5 29 3097.0 30 3313.0 31 3482.0 32 3700.0 33 3943.0 34 4178.0 35 4429.5 36 4758.0 37 5044.5 38 5151.5 39 5088.0 40 4851.5 41 4587.5 42 4466.5 43 4584.0 44 5074.0 45 5853.5 46 8802.5 47 17268.0 48 56253.5 49 60906.0 50 25040.5 51 13262.5 52 7275.0 53 5171.5 54 4273.0 55 3578.0 56 3266.5 57 3073.5 58 2854.0 59 2773.0 60 2726.0 61 2703.0 62 2392.0 63 1531.5 64 871.5 65 648.5 66 549.0 67 450.0 68 355.5 69 291.0 70 250.0 71 199.0 72 162.0 73 185.0 74 163.0 75 107.5 76 78.5 77 46.5 78 30.5 79 23.0 80 13.0 81 7.0 82 4.0 83 2.5 84 2.5 85 2.0 86 2.0 87 2.0 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13867190138086596 2 0.05312754663292917 3 0.15082820442399383 4 0.1280914153988843 5 0.19382549852098313 6 0.19652689919723376 7 0.1668114917584768 8 0.07113688447460008 9 0.12584024816867545 10-11 0.10254066733601372 12-13 0.15499286379988023 14-15 0.13945980991143908 16-17 0.13416956692044826 18-19 0.13180584132872894 20-21 0.13259374985930206 22-23 0.1986655080659322 24-25 0.21431112031588379 26-27 0.14722633685565967 28-29 0.12842909048341566 30-31 0.09770065779106467 32-33 0.1241518727460188 34-35 0.09421134858424093 36-37 0.08757040525512477 38-39 0.05166428793329342 40-41 0.05672941420126335 42-43 0.06089407357714975 44-45 0.04007077669771777 46-47 0.050538704318188986 48-49 0.043222410820010174 50-51 0.053915455163502275 52-53 0.024312606086255725 54-55 0.05245219646386652 56-57 0.025213072978339267 58-59 0.05031358759516809 60-61 0.03635635076787314 62-63 0.04963823742610544 64-65 0.038044726190529794 66-67 0.03646890912938358 68-69 0.06550896639907793 70-71 0.053577780078970946 72-73 0.053915455163502275 74-75 0.0453610196887086 76-77 0.021273530325473758 78-79 0.021273530325473758 80-81 0.023749814278703506 82-83 0.021273530325473758 84-85 0.018909804733754452 86-87 0.022624230663599074 88-89 0.06697222509871369 90-91 0.11199556970289096 92-93 0.08115457864902952 94-95 0.0790159697803311 96-97 0.06933595069043298 98-99 0.05695453092428424 100-101 0.13473235872800046 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 444214.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.716813445017717 #Duplication Level Percentage of deduplicated Percentage of total 1 78.62002770466435 21.79096640932306 2 10.87286518199543 6.027223517243919 3 4.023431458238016 3.3455009741039596 4 1.9493361022747968 2.1611754035355406 5 1.094738563134474 1.517133226273248 6 0.681020788106478 1.1325435681675715 7 0.4317761327353467 0.8377220964725769 8 0.3230116386008202 0.7162282662134734 9 0.25240196555571415 0.6296200373217166 >10 1.4567868042923633 7.552726986014606 >50 0.14202655804513886 2.729023113136745 >100 0.10956334477767854 6.037952390604723 >500 0.012985285306984123 2.389357361336837 >1k 0.0211010886238492 14.115698845660814 >5k 0.0032463213267460308 5.658921402669065 >10k+ 0.005681062321805554 23.358206401922153 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 20531 4.621871440341818 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18335 4.127515116587951 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 15889 3.5768796120788626 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14253 3.208588653216693 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 12229 2.752952405822419 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11763 2.648048012894686 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10840 2.4402652775464078 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 7266 1.63569810946976 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 6354 1.4303916580747118 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 6294 1.4168846546934586 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 5243 1.180286978798507 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4936 1.1111761448310948 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4181 0.9412130189503258 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4022 0.9054194599900047 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 4000 0.9004668920835454 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3969 0.8934882736698978 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 3813 0.8583700648786395 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3217 0.7242004979581914 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2923 0.6580161813900507 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2848 0.6411324271634843 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2699 0.6075900354333722 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2648 0.5961090825593071 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2646 0.5956588491132653 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2612 0.5880048805305551 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1882 0.42366967272530803 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1770 0.39845659974696884 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1730 0.38945193082613333 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1718 0.3867505301498827 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1548 0.3484806872363321 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1418 0.31921551324361686 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1311 0.29512802388038195 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1239 0.27891961982287816 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1179 0.265412616441625 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1126 0.253481430121518 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1119 0.2519056130603718 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1103 0.2483037454920376 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1095 0.24650281170787053 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 864 0.1945008486900458 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 859 0.19337526507494135 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 817 0.18392036270806414 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 760 0.1710887094958736 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 748 0.168387308819623 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 725 0.1632096241901426 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 694 0.15623100577649512 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 658 0.1481268037477432 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 657 0.1479016870247223 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 613 0.13799655121180332 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 576 0.1296672324600305 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 571 0.1285416488449261 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 532 0.11976209664711153 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 532 0.11976209664711153 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 509 0.11458441201763114 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 507 0.11413417857158938 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 486 0.10940672738815076 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 468 0.10535462637377481 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 466 0.10490439292773304 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.2511672302088633E-4 0.0 12-13 0.0 0.0 0.0 4.5023344604177266E-4 0.0 14-15 0.0 0.0 0.0 4.5023344604177266E-4 0.0 16-17 0.0 0.0 0.0 4.5023344604177266E-4 0.0 18-19 0.0 0.0 0.0 4.5023344604177266E-4 0.0 20-21 0.0 0.0 0.0 0.0015758170611462042 0.0 22-23 0.0 0.0 0.0 0.006190709883074375 0.0 24-25 0.0 0.0 0.0 0.013957236827294953 0.0 26-27 0.0 0.0 0.0 0.0328670415610494 0.0 28-29 0.0 0.0 0.0 0.06978618413647476 0.0 30-31 0.0 0.0 0.0 0.15555565560743245 0.0 32-33 0.0 0.0 0.0 0.2657502915261563 0.0 34-35 0.0 0.0 0.0 0.4417915689284894 0.0 36-37 0.0 0.0 0.0 0.6957232324960492 0.0 38-39 0.0 0.0 0.0 0.9548325806930893 0.0 40-41 0.0 0.0 0.0 1.2276740489944036 0.0 42-43 0.0 0.0 0.0 1.4682112675422205 0.0 44-45 0.0 0.0 0.0 1.7158396628651955 0.0 46-47 0.0 0.0 0.0 2.0186216553282876 0.0 48-49 0.0 0.0 0.0 2.383986096791186 0.0 50-51 0.0 0.0 0.0 2.710292786809961 0.0 52-53 0.0 0.0 0.0 2.954319314564602 0.0 54-55 0.0 0.0 0.0 3.1917048989901264 0.0 56-57 0.0 0.0 0.0 3.428077458162057 0.0 58-59 0.0 0.0 0.0 3.8031219187148535 0.0 60-61 0.0 0.0 0.0 4.36850256858181 0.0 62-63 0.0 0.0 0.0 4.793410383283733 0.0 64-65 0.0 0.0 0.0 5.052857406565304 0.0 66-67 0.0 0.0 0.0 5.346972405192092 0.0 68-69 0.0 0.0 0.0 5.657746041322426 0.0 70-71 0.0 0.0 0.0 6.032565385152202 0.0 72-73 0.0 0.0 0.0 6.4721057868504825 0.0 74-75 0.0 0.0 0.0 6.891835916922925 0.0 76-77 0.0 0.0 0.0 7.208912821297843 0.0 78-79 0.0 0.0 0.0 7.538933937246462 0.0 80-81 0.0 0.0 0.0 7.899120694079881 0.0 82-83 0.0 0.0 0.0 8.270788403787364 0.0 84-85 0.0 0.0 0.0 8.92036270806413 0.0 86-87 0.0 0.0 0.0 9.596500785657362 0.0 88-89 0.0 0.0 0.0 9.976272697393599 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACATAT 25 5.2276828E-5 76.12883 1 CATATAG 25 5.2394E-5 76.09445 3 AAGGCGT 35 2.770574E-4 54.34705 6 GGGACTA 45 1.5073732E-5 52.819523 9 GTATTAT 50 2.796029E-5 47.580517 1 GCCCTAA 20 5.3740176E-4 47.524166 28-29 GTCTATA 45 9.5388124E-4 42.293793 1 ACGATGA 45 9.5653546E-4 42.269928 6 AGGCGTG 50 0.0016067022 38.03864 7 CCTCTAG 55 0.002556597 34.604015 1 ACTATAC 55 0.0025622668 34.58839 3 AAAGGCG 55 0.0025622668 34.58839 5 GTCACTA 85 1.616151E-5 33.571083 2 TACATGA 115 1.0227268E-7 33.084545 2 CGTCACT 75 3.045014E-4 31.720348 1 ACATGAG 120 1.485987E-7 31.706026 3 CTTATGT 75 3.0530925E-4 31.706024 2 TGGGTAC 60 0.0039265887 31.702446 6 AACGCCC 30 0.0039362945 31.689928 24-25 CAACGAC 30 0.003946088 31.67385 94-95 >>END_MODULE