##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938005_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 61893 Sequences flagged as poor quality 0 Sequence length 101 %GC 31 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.209005864960496 33.0 31.0 34.0 30.0 34.0 2 32.37091431987462 34.0 31.0 34.0 30.0 34.0 3 32.34234889244341 34.0 31.0 34.0 30.0 34.0 4 35.873798329374885 37.0 35.0 37.0 35.0 37.0 5 35.90082884978915 37.0 35.0 37.0 35.0 37.0 6 36.028306916775726 37.0 35.0 37.0 35.0 37.0 7 35.97820432035933 37.0 35.0 37.0 35.0 37.0 8 36.03678929765886 37.0 35.0 37.0 35.0 37.0 9 37.583054626532885 39.0 37.0 39.0 35.0 39.0 10-11 37.64282713715606 39.0 37.0 39.0 35.0 39.0 12-13 37.602337905740555 39.0 37.0 39.0 35.0 39.0 14-15 38.56175981128722 40.0 38.0 41.0 34.0 41.0 16-17 38.43912074063303 40.0 38.0 41.0 34.5 41.0 18-19 38.37517974569014 40.0 37.0 41.0 35.0 41.0 20-21 38.392588822645536 40.0 37.0 41.0 35.0 41.0 22-23 38.172628568658816 40.0 35.5 41.0 34.5 41.0 24-25 37.962241287383065 40.0 35.0 41.0 34.0 41.0 26-27 37.531190926276 39.5 35.0 41.0 33.5 41.0 28-29 37.18212075678994 39.0 35.0 41.0 33.0 41.0 30-31 36.54090123277269 37.5 35.0 40.0 33.0 41.0 32-33 35.74971321474157 35.5 35.0 40.0 32.5 41.0 34-35 35.17666779765078 35.0 35.0 40.0 31.0 41.0 36-37 34.6681611813937 35.0 35.0 40.0 30.0 41.0 38-39 34.027394051023535 35.0 35.0 40.0 25.5 41.0 40-41 33.688583523177094 35.0 35.0 40.0 23.5 41.0 42-43 33.159000210039906 35.0 34.5 40.0 18.5 41.0 44-45 32.50163184851276 35.0 33.0 40.0 5.0 41.0 46-47 31.55972403987527 35.0 33.0 40.0 2.0 41.0 48-49 30.714886982372803 35.0 33.0 39.5 2.0 41.0 50-51 29.862165349878016 35.0 31.0 39.0 2.0 41.0 52-53 29.142479763462752 35.0 28.5 38.0 2.0 41.0 54-55 28.573812870599262 35.0 26.5 38.0 2.0 41.0 56-57 27.902840385827155 35.0 22.5 38.0 2.0 41.0 58-59 27.210047986040422 35.0 10.0 38.0 2.0 41.0 60-61 26.34017578724573 35.0 2.0 37.0 2.0 40.0 62-63 25.606078231787116 35.0 2.0 36.5 2.0 40.0 64-65 24.768770297125684 34.5 2.0 36.0 2.0 39.5 66-67 23.808920233305866 33.5 2.0 35.5 2.0 39.0 68-69 22.943951658507423 33.0 2.0 35.0 2.0 39.0 70-71 22.156560515728756 33.0 2.0 35.0 2.0 37.5 72-73 21.715331297561924 32.5 2.0 35.0 2.0 37.0 74-75 21.34495823437222 32.0 2.0 35.0 2.0 36.5 76-77 20.32758147124877 29.5 2.0 34.5 2.0 35.0 78-79 20.748267170762446 31.0 2.0 35.0 2.0 35.0 80-81 20.615990499733414 31.0 2.0 35.0 2.0 35.0 82-83 20.369767178840902 31.0 2.0 35.0 2.0 35.0 84-85 20.06885269739712 30.0 2.0 35.0 2.0 35.0 86-87 19.775887418609535 29.5 2.0 35.0 2.0 35.0 88-89 19.404318743638214 29.0 2.0 35.0 2.0 35.0 90-91 19.158394325691113 27.5 2.0 35.0 2.0 35.0 92-93 18.943063028129192 27.0 2.0 35.0 2.0 35.0 94-95 18.765757032297675 27.0 2.0 35.0 2.0 35.0 96-97 18.393412825359896 25.0 2.0 35.0 2.0 35.0 98-99 17.98200119561178 23.5 2.0 34.0 2.0 35.0 100-101 16.783246893832903 10.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.37582854899807927 1101 2 0.1889270327567374 1101 3 -0.08905528087124992 1101 4 -0.5274725155893023 1101 5 -0.15515042422853753 1101 6 -0.44949589776739884 1101 7 0.04904663749766769 1101 8 0.37149380014892586 1101 9 -0.25283633765341307 1101 10-11 -0.24755051907147418 1101 12-13 -0.507407058370724 1101 14-15 -0.6531053786477514 1101 16-17 0.07773762803491735 1101 18-19 -0.03808434002669259 1101 20-21 -0.17127166793358128 1101 22-23 -0.4492227686400625 1101 24-25 -0.4682782216819774 1101 26-27 0.05165356220377504 1101 28-29 -0.12663029432057726 1101 30-31 -0.2521528238034634 1101 32-33 -0.8452817995179203 1101 34-35 -0.5932673322873683 1101 36-37 -1.2222825853430166 1101 38-39 -0.49788676811323995 1101 40-41 -0.31438629343703894 1101 42-43 -0.6304827486016649 1101 44-45 -0.6088691724146535 1101 46-47 -0.2466516595454067 1101 48-49 -0.49866339796031767 1101 50-51 0.31365421374963276 1101 52-53 -0.08871008688818094 1101 54-55 1.0155518488159636 1101 56-57 -0.9492827779046529 1101 58-59 -1.9816625307550133 1101 60-61 -1.224666300543415 1101 62-63 -0.5577727949827214 1101 64-65 -0.7608942249250035 1101 66-67 -0.1815341661627805 1101 68-69 -0.3779433868099815 1101 70-71 0.29681944749764 1101 72-73 0.32232139844882823 1101 74-75 -0.8136707851407117 1101 76-77 -1.9129267770556417 1101 78-79 -2.507698020604561 1101 80-81 -2.027298236016268 1101 82-83 -2.0349245698399976 1101 84-85 -1.2224751487097443 1101 86-87 -1.886682298000263 1101 88-89 -1.720292807177966 1101 90-91 -1.490198813215347 1101 92-93 -1.1255352291150658 1101 94-95 -3.224690847864256 1101 96-97 -2.7946989542302347 1101 98-99 -2.352406438048373 1101 100-101 -1.6279467819287792 1102 1 0.27378773267154344 1102 2 0.4746413184710221 1102 3 0.23747533137365195 1102 4 0.26844585175764024 1102 5 0.17138018801636434 1102 6 0.42805512264076384 1102 7 0.25312827015072514 1102 8 0.4327182899448445 1102 9 0.4002248868363836 1102 10-11 0.4871433584795426 1102 12-13 0.5130011048945917 1102 14-15 0.36730278461755717 1102 16-17 0.8430437504838935 1102 18-19 0.7884462722182022 1102 20-21 0.45117731165825603 1102 22-23 0.5609813129925882 1102 24-25 0.052129941583331174 1102 26-27 0.5924698887343922 1102 28-29 0.13867582812839885 1102 30-31 -0.4256222115585686 1102 32-33 -0.9065062893138389 1102 34-35 -0.787144883307775 1102 36-37 -1.2324866669756673 1102 38-39 -1.2529888089295724 1102 40-41 -0.48785568119213707 1102 42-43 -1.0182378506424854 1102 44-45 -2.8231548867003653 1102 46-47 -3.5629781901576543 1102 48-49 -3.0190715612256227 1102 50-51 -2.002672316862615 1102 52-53 -1.629526413418791 1102 54-55 -1.8517950899595448 1102 56-57 -1.4288746146393443 1102 58-59 -0.859213551163176 1102 60-61 -0.592013239318927 1102 62-63 -1.1292013664112908 1102 64-65 -0.5976289188025525 1102 66-67 -0.7019423294280891 1102 68-69 0.1526688580879778 1102 70-71 0.12335005974253832 1102 72-73 0.32232139844882823 1102 74-75 0.9006149291450001 1102 76-77 0.9850324066178224 1102 78-79 0.9106693263342116 1102 80-81 0.39106911092250485 1102 82-83 0.1793611444457177 1102 84-85 0.2469126063922964 1102 86-87 0.03168504893851676 1102 88-89 -0.4345785214636777 1102 90-91 0.15265832964179893 1102 92-93 -1.0030862495232284 1102 94-95 -1.8981602356193576 1102 96-97 -0.6926581379037025 1102 98-99 -0.7095492951912306 1102 100-101 -1.1891712717246978 1103 1 -0.3902625028072606 1103 2 -0.018294317321753795 1103 3 0.14446120264523188 1103 4 0.10400315160065077 1103 5 0.2435936888012904 1103 6 0.09210535811956788 1103 7 0.27510629212874704 1103 8 -0.055900234387962655 1103 9 0.3809941176056171 1103 10-11 0.31602875879352155 1103 12-13 0.30165887569521743 1103 14-15 0.2056073372078231 1103 16-17 0.3915131382389987 1103 18-19 -0.02022719716955379 1103 20-21 0.004552507890593915 1103 22-23 0.3930064307319938 1103 24-25 0.14651769668537185 1103 26-27 0.27418103473124944 1103 28-29 -0.32423625978368165 1103 30-31 0.24039034731112707 1103 32-33 -0.5623147665508839 1103 34-35 -0.13035524437528068 1103 36-37 0.41959339581867994 1103 38-39 -0.9976905357741543 1103 40-41 -0.5949985383349983 1103 42-43 -0.21682497780103915 1103 44-45 -0.8276682304994232 1103 46-47 0.11677063244831132 1103 48-49 1.1512581091040488 1103 50-51 0.1762915763869941 1103 52-53 1.0959445441950244 1103 54-55 1.9904341094125115 1103 56-57 1.9653561545914258 1103 58-59 1.1706137643784267 1103 60-61 2.3781594451394703 1103 62-63 3.2301000464615512 1103 64-65 3.0534699822963454 1103 66-67 3.02902313368023 1103 68-69 3.967817994665687 1103 70-71 4.228923058172679 1103 72-73 4.60548466375495 1103 74-75 4.768158100259601 1103 76-77 4.584129737858014 1103 78-79 4.661650488029661 1103 80-81 4.351037713748251 1103 82-83 4.333992227648228 1103 84-85 3.844243846580678 1103 86-87 4.322501375469127 1103 88-89 4.037948951063793 1103 90-91 3.8997148445554544 1103 92-93 4.2783109247310875 1103 94-95 4.67935153832099 1103 96-97 4.747687231013092 1103 98-99 3.84088241595477 1103 100-101 4.331629356218791 1104 1 0.102869365324608 1104 2 0.4363760123485747 1104 3 0.3216590048430348 1104 4 -0.04022761763011573 1104 5 0.2147475349551371 1104 6 0.384687775701984 1104 7 0.23527112729358635 1104 8 0.12404482055708854 1104 9 0.2930820296935295 1104 10-11 0.27413315439791575 1104 12-13 0.22473579877213723 1104 14-15 0.3086293152298012 1104 16-17 -0.054915433189570706 1104 18-19 0.006558517116154405 1104 20-21 0.13229976063784932 1104 22-23 0.4232262109517748 1104 24-25 0.6018748395425106 1104 26-27 0.36415356220377504 1104 28-29 0.7547472566998294 1104 30-31 0.3983573802781635 1104 32-33 0.27971820048207974 1104 34-35 0.6031612391412082 1104 36-37 0.9154725166978039 1104 38-39 1.5709907829071597 1104 40-41 1.8782157473792935 1104 42-43 1.6169662309901724 1104 44-45 0.7444471541159601 1104 46-47 -2.015784312606634 1104 48-49 -1.7690715612256227 1104 50-51 -2.159560071964652 1104 52-53 -2.4267202909698113 1104 54-55 -2.43470325322485 1104 56-57 -3.1342317574964866 1104 58-59 -2.5594686532039894 1104 60-61 -3.3828295658495406 1104 62-63 -2.9608340194725145 1104 64-65 -2.6065574902311255 1104 66-67 -3.5705647784076824 1104 68-69 -3.2656984888507985 1104 70-71 -2.3383846341350143 1104 72-73 -1.9391581933879074 1104 74-75 -2.2524462953447966 1104 76-77 -1.9996614709331944 1104 78-79 -1.9056572042780324 1104 80-81 -2.2938798686693325 1104 82-83 -2.277271508615506 1104 84-85 -2.509464944628114 1104 86-87 -2.3560700531023038 1104 88-89 -3.13993566432082 1104 90-91 -2.523362078521469 1104 92-93 -2.10767808625792 1104 94-95 -2.9058132968438457 1104 96-97 -2.6135765052506432 1104 98-99 -3.168732968660617 1104 100-101 -2.298865149275718 1105 1 0.3398548374795709 1105 2 0.444547925181503 1105 3 -0.03578651573807434 1105 4 0.20341645016649323 1105 5 0.32703567054835503 1105 6 0.2941914076632486 1105 7 0.3572444929110148 1105 8 0.25146612806314295 1105 9 0.6375130224296015 1105 10-11 0.16804961928895068 1105 12-13 0.273464612331459 1105 14-15 0.3470675718884024 1105 16-17 0.49108940942543455 1105 18-19 1.1132473791016295 1105 20-21 1.0260648938097603 1105 22-23 0.9262528453343393 1105 24-25 0.9282973577023199 1105 26-27 0.8494743854483318 1105 28-29 0.9256007675957179 1105 30-31 0.8332847410529851 1105 32-33 0.31089253462251065 1105 34-35 0.6367568807877007 1105 36-37 1.2962231220246778 1105 38-39 1.0083394511880286 1105 40-41 0.8244929870887319 1105 42-43 1.5367604198521505 1105 44-45 1.8641505439464652 1105 46-47 2.063967503373995 1105 48-49 1.7751173007598453 1105 50-51 1.6247922282774745 1105 52-53 1.3803317671415662 1105 54-55 1.7609686596080785 1105 56-57 1.935532165021673 1105 58-59 2.417680257207632 1105 60-61 2.5802454946831475 1105 62-63 2.6099892250795413 1105 64-65 3.1796107906405453 1105 66-67 3.3212330424155674 1105 68-69 3.999434683062038 1105 70-71 3.871361128576847 1105 72-73 3.5694677146024105 1105 74-75 4.240289782137044 1105 76-77 3.976730780882786 1105 78-79 3.8146817787728153 1105 80-81 4.205666136173285 1105 82-83 4.433079580972869 1105 84-85 4.419863142538958 1105 86-87 3.0894505280115006 1105 88-89 3.710699928899519 1105 90-91 3.711807413003953 1105 92-93 3.789719008173069 1105 94-95 3.2222133375387223 1105 96-97 4.041689838575021 1105 98-99 3.5770297432037914 1105 100-101 2.46233339793978 1106 1 -0.0489163489611073 1106 2 0.6127152980628594 1106 3 0.20335543341446538 1106 4 0.27227238236988427 1106 5 0.3709975349551371 1106 6 0.2507592042734146 1106 7 0.32678898443644044 1106 8 0.44770553484280384 1106 9 0.19709988683638358 1106 10-11 0.26855279725505454 1106 12-13 0.06848579877213723 1106 14-15 0.1947900295155165 1106 16-17 0.2587006382389987 1106 18-19 0.5657103028304462 1106 20-21 0.722701546352134 1106 22-23 0.8082708538089136 1106 24-25 0.5762051966853718 1106 26-27 0.8931714193466362 1106 28-29 0.8752829709855448 1106 30-31 1.139428808849594 1106 32-33 0.9370842719106491 1106 34-35 1.271688024855493 1106 36-37 1.8150260881263733 1106 38-39 1.2685354257643056 1106 40-41 1.3737514616650017 1106 42-43 0.48750194527588064 1106 44-45 -0.044615345884039925 1106 46-47 0.5110014016790814 1106 48-49 0.430705224488662 1106 50-51 0.14624349946391746 1106 52-53 0.544261851887331 1106 54-55 0.2885110324894349 1106 56-57 0.6414378853606557 1106 58-59 1.02758491822458 1106 60-61 0.864938291293317 1106 62-63 1.564835623384628 1106 64-65 2.024022866911732 1106 66-67 2.5879173644494635 1106 68-69 2.237649725434917 1106 70-71 1.8256778658649857 1106 72-73 1.7304846637549502 1106 74-75 1.909384061798061 1106 76-77 1.5360528147810903 1106 78-79 1.2662177957219676 1106 80-81 1.1971915599020981 1106 82-83 1.565362419955921 1106 84-85 2.2829457696576014 1106 86-87 2.041251375469127 1106 88-89 1.99287683567918 1106 90-91 1.695387921478531 1106 92-93 1.4291522708849342 1106 94-95 1.8464188460132966 1106 96-97 2.2524949233207856 1106 98-99 2.221892031339383 1106 100-101 1.8953312792957107 1107 1 -0.21946719641873358 1107 2 -0.08087580363205404 1107 3 -0.3747695665855346 1107 4 -0.35590558373181125 1107 5 0.10669668749751082 1107 6 -0.06174079572658542 1107 7 0.25554957765677955 1107 8 -0.5281948888860128 1107 9 -0.4302835877398863 1107 10-11 -0.42517071969409415 1107 12-13 -0.15873877749904608 1107 14-15 0.1182540125663678 1107 16-17 -0.593656353286427 1107 18-19 -0.30200685818650896 1107 20-21 0.009115741267386568 1107 22-23 0.20591386228348796 1107 24-25 0.37744990007519874 1107 26-27 0.3748981142618888 1107 28-29 0.6544143269177525 1107 30-31 0.5620983003750197 1107 32-33 -0.4602939060554547 1107 34-35 -0.2700227802292545 1107 36-37 -0.6020819627210869 1107 38-39 0.24562758678125363 1107 40-41 0.7312726481056799 1107 42-43 0.2655739791741851 1107 44-45 -0.43245962554505724 1107 46-47 -0.9529816491683789 1107 48-49 -0.7757301568672688 1107 50-51 -0.2904620090106569 1107 52-53 -1.2637360294685998 1107 54-55 -0.6034381200529388 1107 56-57 -0.9119254620969706 1107 58-59 -1.4552010987245723 1107 60-61 -0.8434833188761743 1107 62-63 -0.4239090800052061 1107 64-65 -0.7186942941052159 1107 66-67 -0.5770720423301974 1107 68-69 -0.17853141863288258 1107 70-71 0.10864926417007226 1107 72-73 0.10336601968715442 1107 74-75 -0.27665937040532995 1107 76-77 -0.3368285411511138 1107 78-79 -0.21074195004074525 1107 80-81 -0.2689101350131544 1107 82-83 -0.11776787665424848 1107 84-85 -0.28352668796951974 1107 86-87 0.047077646655569794 1107 88-89 -0.39099498635471974 1107 90-91 0.5592650401226003 1107 92-93 0.5693800251222214 1107 94-95 -0.10829513703755111 1107 96-97 0.39762204196485484 1107 98-99 0.09397889574616158 1107 100-101 0.250468991160119 1108 1 0.4454018328570726 1108 2 0.2789084798810393 1108 3 0.24644255462659004 1108 4 0.26659056418806415 1108 5 0.46853541374301244 1108 6 0.3170470830612899 1108 7 0.24014125716371382 1108 8 0.3118153833276551 1108 9 0.5062854928969927 1108 10-11 0.43024787301263245 1108 12-13 0.5239782230145593 1108 14-15 0.7463998780003607 1108 16-17 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78-79 2.058198217408716 2316 80-81 1.88996264423945 2316 82-83 2.140757751281221 2316 84-85 2.3094894443563945 2316 86-87 2.5845495682402095 2316 88-89 2.332202889896049 2316 90-91 2.607662017864076 2316 92-93 2.705790072089755 2316 94-95 3.334935412278355 2316 96-97 2.3918021522364477 2316 98-99 2.060182693989983 2316 100-101 2.263742423874021 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 1.0 5 3.0 6 16.0 7 29.0 8 77.0 9 133.0 10 291.0 11 463.0 12 641.0 13 943.0 14 1191.0 15 1500.0 16 1863.0 17 2319.0 18 2271.0 19 1779.0 20 1702.0 21 1717.0 22 1723.0 23 1689.0 24 1807.0 25 1777.0 26 1323.0 27 865.0 28 876.0 29 978.0 30 1132.0 31 1263.0 32 1392.0 33 1908.0 34 3062.0 35 8891.0 36 5168.0 37 6498.0 38 4222.0 39 379.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.16354030342688 25.542468453621574 15.323219103937442 12.970772139014105 2 14.260552000517096 29.262814297718307 37.79975437916101 18.676879322603583 3 20.59850051706308 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33.34221963711567 38.549593653563406 16.079362771234226 48-49 11.902799993537233 32.44066372610796 39.031069749406235 16.625466530948575 50-51 10.993973470343981 32.73795097991695 40.67665164073482 15.591423909004249 52-53 10.877643675375245 33.17095632785614 41.0426057874073 14.908794209361318 54-55 10.184511980353191 32.21608259415443 39.44226326078878 18.1571421647036 56-57 12.848787423456612 33.75906806908697 35.93136542096845 17.46077908648797 58-59 10.988318549755222 34.688090737240074 37.802336290048956 16.521254422955746 60-61 11.892297998158112 36.25208020293086 34.89328357003215 16.96233822887887 62-63 11.157158321619569 37.47839012489296 34.27285799686556 17.091593556621913 64-65 10.986702858158434 37.60360622364403 34.22761863215549 17.18207228604204 66-67 10.663564538800834 36.29974310503611 34.40292117040699 18.63377118575606 68-69 10.8672294704528 36.04475501878257 34.44036030213677 18.647655208627864 70-71 11.922996138497084 35.003958444412135 33.427043445947035 19.646001971143747 72-73 11.798587885544407 32.66201347486792 34.243775548123374 21.295623091464304 74-75 12.405280080138304 32.42127542694651 33.81965650396652 21.353787988948667 76-77 12.922301391110466 30.873442877223596 35.08393517845313 21.120320553212803 78-79 12.41658992131582 30.711065871746403 35.84492592054029 21.02741828639749 80-81 12.456174365437125 31.41873879113955 34.71878887757905 21.406297965844278 82-83 12.060329924224064 31.424393711728303 34.54833341411791 21.96694294992972 84-85 12.87667027516117 30.991581974762084 33.5024478518686 22.629299898208142 86-87 13.285832000387765 30.942917615885477 33.497326030407315 22.273924353319437 88-89 13.826280839513352 30.49294750618002 32.853472929087296 22.82729872521933 90-91 13.499103291163783 30.008240027143618 33.55064385310132 22.94201282859128 92-93 13.285939330290422 30.889041483216868 32.98218685624268 22.84283233025003 94-95 13.11295299953145 30.91221947554651 32.45924417947103 23.51558334545102 96-97 12.932803386489585 30.57857916080978 31.953532709676374 24.535084743024253 98-99 13.691370591181556 30.52687702971257 31.388040650800576 24.393711728305302 100-101 14.020971676926308 30.748226778472525 31.920411031942226 23.310390512658945 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4922.0 1 2737.5 2 700.5 3 868.0 4 577.5 5 341.5 6 683.5 7 1184.5 8 1431.5 9 1368.0 10 940.5 11 470.5 12 351.5 13 348.5 14 345.5 15 319.0 16 312.5 17 334.5 18 364.0 19 388.0 20 423.0 21 485.0 22 576.0 23 647.0 24 686.5 25 785.0 26 859.0 27 996.0 28 1123.0 29 1170.0 30 1235.0 31 1278.0 32 1268.5 33 1231.0 34 1205.5 35 1131.5 36 1030.5 37 936.5 38 831.0 39 785.0 40 799.5 41 863.5 42 948.0 43 1012.0 44 1029.0 45 1040.5 46 1201.5 47 1698.0 48 3595.5 49 4074.5 50 3207.5 51 2740.0 52 1532.5 53 704.5 54 391.5 55 331.5 56 284.5 57 227.0 58 192.0 59 150.0 60 127.5 61 121.5 62 92.5 63 56.5 64 24.0 65 16.0 66 18.0 67 20.5 68 21.0 69 23.5 70 24.5 71 23.0 72 19.5 73 16.0 74 18.5 75 21.5 76 13.5 77 1.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014541224371092046 3 0.008078457983940026 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.007270612185546023 26-27 0.026658911347002084 28-29 0.0 30-31 0.0 32-33 8.078457983940026E-4 34-35 0.02504321975021408 36-37 0.008886303782334027 38-39 0.027466757145396085 40-41 0.01615691596788005 42-43 0.01211768697591004 44-45 0.004847074790364015 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 8.078457983940026E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0032313831935760103 86-87 0.0 88-89 0.0 90-91 0.0 92-93 8.078457983940026E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 61893.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.933207309388784 #Duplication Level Percentage of deduplicated Percentage of total 1 86.35318170201892 32.75653143328002 2 6.891558054348752 5.2283780072059844 3 2.406508220461709 2.7385972565556687 4 1.0818638725615468 1.6415426623366134 5 0.7113041996762927 1.349102483317984 6 0.38333759263991823 0.8724734622655227 7 0.3066700741119346 0.8143085647811545 8 0.25129908850839083 0.7626064336839383 9 0.17037226339551922 0.5816489748436818 >10 1.069085952806883 7.813484562066793 >50 0.17889087656529518 5.079734380301488 >100 0.14481642388619134 10.392128350540448 >500 0.021296532924439903 5.404488391255877 >1k 0.02981514609421586 24.564975037564828 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4741 7.659993860371932 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2373 3.834036159177936 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2120 3.425266185190571 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1714 2.7692953968946408 No Hit CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT 1668 2.6949735834423927 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1558 2.517247507795712 No Hit ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT 1030 1.6641623446916454 RNA PCR Primer, Index 46 (95% over 21bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 777 1.25539237070428 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 742 1.1988431648166997 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 705 1.1390625757355437 No Hit ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT 605 0.9774934160567431 RNA PCR Primer, Index 46 (95% over 23bp) GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 516 0.8336968639426106 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 473 0.7642221252807264 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 451 0.7286769101513904 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 413 0.667280629473446 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 358 0.5784175916501058 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 296 0.4782447126492495 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 266 0.4297739647456093 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 260 0.42007981516488135 No Hit CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT 256 0.4136170487777293 RNA PCR Primer, Index 46 (96% over 26bp) GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 252 0.40715428239057727 No Hit CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG 198 0.319906936164025 TruSeq Adapter, Index 4 (95% over 23bp) TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 188 0.30375002019614494 No Hit TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC 188 0.30375002019614494 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 172 0.2778989546475369 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 169 0.2730518798571729 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 166 0.2682048050668088 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 146 0.23589097313104876 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 145 0.23427528153426072 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 145 0.23427528153426072 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 142 0.22942820674389672 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 140 0.22619682355032072 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 136 0.21973405716316868 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 125 0.20196144959850065 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 121 0.19549868321134864 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 120 0.19388299161456063 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 117 0.1890359168241966 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 115 0.18580453363062058 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 115 0.18580453363062058 No Hit TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT 114 0.1841888420338326 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 114 0.1841888420338326 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 112 0.18095745884025657 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 108 0.17449469245310456 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 106 0.17126330925952854 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 103 0.16641623446916454 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 102 0.16480054287237653 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 100 0.1615691596788005 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 98 0.1583377764852245 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 97 0.1567220848884365 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 96 0.1551063932916485 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 96 0.1551063932916485 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 93 0.15025931850128446 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 91 0.14702793530770847 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 90 0.14541224371092046 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 90 0.14541224371092046 No Hit GGTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 89 0.14379655211413245 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 89 0.14379655211413245 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 89 0.14379655211413245 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 88 0.14218086051734444 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 86 0.13894947732376844 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 85 0.13733378572698043 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 82 0.1324867109366164 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 79 0.1276396361462524 No Hit CTTTACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC 78 0.1260239445494644 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 77 0.12440825295267639 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 75 0.12117686975910039 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 75 0.12117686975910039 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 73 0.11794548656552438 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 73 0.11794548656552438 No Hit GTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 72 0.11632979496873637 No Hit ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGC 71 0.11471410337194836 TruSeq Adapter, Index 18 (95% over 24bp) CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 70 0.11309841177516036 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 70 0.11309841177516036 No Hit CTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC 69 0.11148272017837235 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 66 0.10663564538800833 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 65 0.10501995379122034 No Hit GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAAGGCG 62 0.10017287900085632 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0016156915967880051 0.0 5 0.0 0.0 0.0 0.0016156915967880051 0.0 6 0.0 0.0 0.0 0.0016156915967880051 0.0 7 0.0 0.0 0.0 0.0016156915967880051 0.0 8 0.0 0.0 0.0 0.0016156915967880051 0.0016156915967880051 9 0.0 0.0 0.0 0.0016156915967880051 0.0016156915967880051 10-11 0.0 0.0 0.0 0.0016156915967880051 0.0016156915967880051 12-13 0.0 0.0 0.0 0.0016156915967880051 0.0016156915967880051 14-15 0.0 0.0 0.0 0.0016156915967880051 0.0016156915967880051 16-17 0.0 0.0 0.0 0.0024235373951820077 0.0016156915967880051 18-19 0.0 0.0 0.0 0.0032313831935760103 0.0016156915967880051 20-21 0.0 0.0 0.0 0.004847074790364015 0.0016156915967880051 22-23 0.0 0.0 0.0 0.023427528153426073 0.0016156915967880051 24-25 0.0 0.0 0.0 0.05493351429079217 0.0016156915967880051 26-27 0.0 0.0 0.0 0.11794548656552437 0.0016156915967880051 28-29 0.0 0.0 0.0 0.30778924918811496 0.0016156915967880051 30-31 0.0 0.0 0.0 0.6656649378766581 0.0016156915967880051 32-33 0.0 0.0 0.0 1.1657214870825459 0.0016156915967880051 34-35 0.0 0.0 0.0 1.914594542193786 0.0016156915967880051 36-37 0.0 0.0 0.0 2.825844602782221 0.0016156915967880051 38-39 0.0 0.0 0.0 3.749212350346566 0.0016156915967880051 40-41 0.0 0.0 0.0 4.716203771024187 0.0016156915967880051 42-43 0.0 0.0 0.0 5.437610068990031 0.0016156915967880051 44-45 0.0 0.0 0.0 6.049149338374291 0.0016156915967880051 46-47 0.0 0.0 0.0 6.683308290113583 0.0016156915967880051 48-49 0.0 0.0 0.0 7.321506470844845 0.0016156915967880051 50-51 0.0 0.0 0.0 7.842567010808977 0.0016156915967880051 52-53 0.0 0.0 0.0 8.241642835215615 0.0016156915967880051 54-55 0.0 0.0 0.0 8.694844328114648 0.0016156915967880051 56-57 0.0 0.0 0.0 9.210249947490023 0.0016156915967880051 58-59 0.0 0.0 0.0 9.614980692485418 0.0016156915967880051 60-61 0.0 0.0 0.0 10.093225405134667 0.0016156915967880051 62-63 0.0 0.0 0.0 10.487454154750942 0.0016156915967880051 64-65 0.0 0.0 0.0 10.785549254358328 0.0016156915967880051 66-67 0.0 0.0 0.0 11.119189569095052 0.0016156915967880051 68-69 0.0 0.0 0.0 11.509379089719355 0.0016156915967880051 70-71 0.0 0.0 0.0 11.91814906370672 0.0016156915967880051 72-73 0.0 0.0 0.0 12.313993504919782 0.0016156915967880051 74-75 0.0 0.0 0.0 12.688026109576203 0.0016156915967880051 76-77 0.0 0.0 0.0 13.00227812515147 0.0016156915967880051 78-79 0.0 0.0 0.0 13.389236262582198 0.0016156915967880051 80-81 0.0 0.0 0.0 13.758421792448257 0.0016156915967880051 82-83 0.0 0.0 0.0 14.104179794160892 0.0016156915967880051 84-85 0.0 0.0 0.0 14.550918520672774 0.0016156915967880051 86-87 0.0 0.0 0.0 14.936260966506714 0.0016156915967880051 88-89 0.0 0.0 0.0 15.281211122420952 0.0016156915967880051 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATACA 15 6.588101E-4 95.0 2 GTACCCA 55 1.15887815E-8 60.454544 1 TACCCAT 55 1.15887815E-8 60.454544 2 ACATGGA 25 0.0049989 57.0 3 CATGGAA 25 0.0049989 57.0 4 ACCCATG 75 1.3349927E-7 44.333336 3 CGCAGAA 80 1.0612308E-5 35.625 9 GCAGAGA 55 0.002560877 34.545456 9 GAGTACC 35 2.0427947E-4 33.928574 12-13 ATGGGGA 115 3.7874124E-6 28.913044 5 TGGGGAA 115 3.7874124E-6 28.913044 6 CATGGGG 225 3.6379788E-12 27.444445 4 CAATTCC 35 0.008363159 27.142859 80-81 AGTACTC 215 5.4569682E-11 26.51163 5 AGTAAAA 95 3.2380922E-7 22.5 12-13 GTACAAA 130 2.8981862E-4 21.923077 3 GAGTACT 1425 0.0 21.833332 12-13 GGGGAAA 155 3.8301136E-5 21.451612 7 GTACTTT 1360 0.0 21.30515 14-15 CAGAGAA 80 4.943266E-5 20.78125 10-11 >>END_MODULE