FastQCFastQC Report
Thu 26 May 2016
SRR938004_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938004_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64178
Sequences flagged as poor quality0
Sequence length101
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46927.310916513446976No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24923.8829505438000558No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23143.6055969335286235No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17902.789117766212721No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT16592.5849979743837452No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16002.493066159743214No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT9981.5550500171398298RNA PCR Primer, Index 46 (95% over 21bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7781.2122534201751378No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7671.1951135903269032No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7031.0953909439371747No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT6270.9769703013493721RNA PCR Primer, Index 46 (95% over 23bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA5540.863224157811088No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5030.783757673969273No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA4730.7370126834740877No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4200.6544298669325938No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT3830.5967777119885319No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2970.4627754059023341No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2830.440961077004581No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT2740.42693757985602543RNA PCR Primer, Index 46 (96% over 26bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2710.4222630808065069No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG2440.38019258936084016No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT2300.358378260463087No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC2180.3396802642650129No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT2070.3225404344167784No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG1970.30695877091838325TruSeq Adapter, Index 4 (95% over 23bp)
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1860.28981894107014866No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC1780.27735361027143257No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA1580.2461902832746424No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1460.2274922870765683No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1440.2243759543768893No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1350.21035245722833368No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC1350.21035245722833368No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1350.21035245722833368No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1350.21035245722833368No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT1340.20879429087849422No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA1340.20879429087849422No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG1330.20723612452865467No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA1260.19632896007977813No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG1250.1947707937299386No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC1240.1932126273800991No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC1200.18697996198074107No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1200.18697996198074107No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT1180.18386362928106204No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT1170.18230546293122254No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG1140.17763096388170402No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC1070.16672379943282745No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA1050.16360746673314844No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1040.16204930038330892No Hit
GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1040.16204930038330892No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG1020.1589329676836299No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG1010.1573748013337904No Hit
GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA980.15270030228427187No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT970.15114213593443235No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT970.15114213593443235No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT940.14646763688491382No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGC940.14646763688491382TruSeq Adapter, Index 18 (95% over 24bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA900.1402349714855558No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT900.1402349714855558No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT890.13867680513571629No Hit
CTTTACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC880.1371186387858768No Hit
CTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC870.13556047243603728No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT850.13244413973635827No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA850.13244413973635827No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC800.12465330798716072No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC790.1230951416373212No Hit
GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA780.1215369752874817No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT770.11997880893764219No Hit
TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT770.11997880893764219No Hit
GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT760.11842064258780267No Hit
GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC740.11530430988812367No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA740.11530430988812367No Hit
GGTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA730.11374614353828415No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG720.11218797718844464No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG720.11218797718844464No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG720.11218797718844464No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAAGGCG710.11062981083860514No Hit
CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA700.10907164448876562No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650.10128081273956809No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGC200.00206116371.2845151
GTATATG200.00206116371.2845151
ATGGGAT200.00206755571.228965
TTATATA250.001612808937.98877392-93
ATGGGGG550.00256529934.535255
ACAACAG300.003928658631.68200988-89
TACAACC300.003943694331.65731662-63
GAGTAAA1056.7839195E-527.1560061
AGTAAAA1056.7839195E-527.1560062
CGCAGAA908.870701E-426.3810949
AGTACTC2355.456968E-1226.2893262
CATGGGG1501.0369058E-625.3258534
GGCTTGA1502.9828303E-522.1601228
GGGCTTG1502.9828303E-522.1601227
TGGGCTT1553.841729E-521.4452766
CATGGGC1805.0573544E-621.1048764
ATGGGCT1604.906905E-520.7751125
GTAAAAA1604.906905E-520.7751123
GCTTGAT1656.218627E-520.1455639
AGTACTT13200.019.7858212-13
Produced by FastQC (version 0.11.5)