FastQCFastQC Report
Thu 26 May 2016
SRR938003_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938003_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2310219
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105710.4575756670687931No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85620.37061421449654774No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54200.23460979240496246No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28990.12548593877896425No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26420.11436145231252969No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA25260.10934028332378878No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC25240.10925371144467255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47900.056.684991
GTATCAA88250.042.8795051
ATCAACG110200.033.5087663
TATCAAC111250.033.40612
TCAACGC111150.033.222374
CAACGCA112750.032.835225
AACGCAG116200.031.8936396
ACGCAGA130950.028.006077
CGCAGAG132250.027.731378
GTGGTAT21000.027.4952321
TGGTATC21400.025.761032
GCAGAGT144550.024.5823759
GAGTACT96300.023.23307812-13
CAGAGTA139250.022.15720210-11
GTACTTT102250.021.69530114-15
GTACCGT2005.376405E-721.3810026
TAAGGTG15650.021.2570385
AGAGTAC129700.021.13327210-11
GTGTAAG10250.020.9498041
AGTACTT99850.020.33762212-13
Produced by FastQC (version 0.11.5)