FastQCFastQC Report
Thu 26 May 2016
SRR938003_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938003_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2310219
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97660.42273048572451355No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84960.3677573424857124No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54340.235215795558776No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA27350.11838704469143402No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC27340.1183437587518759No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA27100.11730489620248123No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA26870.11630931959264468No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA24470.105920694098698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49550.054.2396661
GTATCAA88050.041.11191
ATCAACG110300.032.207963
TATCAAC111850.032.0662082
TCAACGC111450.031.7902774
CAACGCA113950.031.0504075
AACGCAG118200.029.8542586
ACGCAGA131650.026.65997
CGCAGAG132200.026.4052838
TGGTATC21600.025.9860652
GTGGTAT23200.025.424121
TAAGGTG14400.025.0991955
GCAGAGT146750.023.5283379
GGACCGA5750.023.127316
GAGTACT96050.022.89383512-13
AGAGTAC129550.021.59299310-11
GTACTTT100750.021.51942414-15
GTAAGGT15250.021.5177754
CAGAGTA139200.021.40964310-11
AGTACTT100650.021.2340912-13
Produced by FastQC (version 0.11.5)