##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938003_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2310219 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.47791140147319 33.0 31.0 34.0 31.0 34.0 2 32.705246991735414 34.0 31.0 34.0 31.0 34.0 3 32.77151300374553 34.0 31.0 34.0 31.0 34.0 4 36.23029288565283 37.0 35.0 37.0 35.0 37.0 5 36.155699524590524 37.0 35.0 37.0 35.0 37.0 6 36.21003333450205 37.0 37.0 37.0 35.0 37.0 7 36.18457124627579 37.0 37.0 37.0 35.0 37.0 8 36.18046947064326 37.0 37.0 37.0 35.0 37.0 9 37.878518443489554 39.0 38.0 39.0 35.0 39.0 10-11 37.844475134175596 39.0 38.0 39.0 35.0 39.0 12-13 37.76435956937416 39.0 38.0 39.0 35.0 39.0 14-15 39.00111288150604 40.0 38.0 41.0 36.0 41.0 16-17 38.90397555383277 40.0 38.0 41.0 35.0 41.0 18-19 39.16486921802651 40.0 38.5 41.0 36.0 41.0 20-21 39.13652645052265 40.0 39.0 41.0 36.0 41.0 22-23 39.02973397760126 40.0 39.0 41.0 35.0 41.0 24-25 38.910776640656145 40.0 38.0 41.0 35.0 41.0 26-27 38.74282286657672 40.0 38.0 41.0 35.0 41.0 28-29 38.61647575403025 40.0 38.0 41.0 34.5 41.0 30-31 38.36176938203694 40.0 38.0 41.0 34.0 41.0 32-33 38.12284333216894 40.0 38.0 41.0 34.0 41.0 34-35 38.01649042796375 40.0 38.0 41.0 33.0 41.0 36-37 37.9488860147025 40.0 37.5 41.0 33.0 41.0 38-39 37.745583860231434 40.0 37.0 41.0 33.0 41.0 40-41 37.51337860176892 40.0 37.0 41.0 33.0 41.0 42-43 37.246385515832046 40.0 36.5 41.0 32.0 41.0 44-45 36.9590344032319 40.0 36.0 41.0 31.0 41.0 46-47 36.569506613875134 39.0 36.0 41.0 30.5 41.0 48-49 36.12916849008687 39.0 35.0 40.5 29.5 41.0 50-51 35.72370173563632 39.0 35.0 40.0 28.5 41.0 52-53 35.172170041022085 38.0 34.5 40.0 27.0 41.0 54-55 34.764002243943104 38.0 34.0 40.0 26.0 41.0 56-57 34.35705662536755 38.0 34.0 40.0 25.0 41.0 58-59 33.82343145822972 37.0 33.0 40.0 23.0 41.0 60-61 33.31528114867032 37.0 32.5 39.5 22.0 41.0 62-63 32.734418901411516 36.0 31.5 39.0 19.5 40.0 64-65 32.132174482159485 35.5 31.0 39.0 17.0 40.0 66-67 31.53634374057178 35.0 30.5 38.0 10.5 40.0 68-69 30.867727475187415 35.0 29.5 38.0 7.5 40.0 70-71 30.215770452931086 34.0 29.0 37.0 4.5 39.0 72-73 29.521375679102285 34.0 28.0 36.5 2.0 39.0 74-75 28.847941472215403 34.0 27.0 36.0 2.0 39.0 76-77 27.54999396161143 31.5 26.0 34.5 2.0 36.5 78-79 28.189170593783533 33.0 27.0 35.0 2.0 37.0 80-81 27.896176076813497 33.0 26.0 35.0 2.0 37.0 82-83 27.310330535763057 33.0 26.0 35.0 2.0 36.0 84-85 26.62439318523482 32.5 24.5 35.0 2.0 36.0 86-87 25.88223865356488 32.0 22.0 35.0 2.0 35.0 88-89 25.038770566773106 31.5 19.0 34.0 2.0 35.0 90-91 24.337527524446816 31.0 13.5 34.0 2.0 35.0 92-93 23.35860647843343 30.5 4.5 34.0 2.0 35.0 94-95 22.42443725032129 30.0 2.0 34.0 2.0 35.0 96-97 21.34754302514177 29.0 2.0 34.0 2.0 35.0 98-99 19.907381291557208 28.5 2.0 34.0 2.0 35.0 100-101 16.115158346459793 13.5 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18066312274054752 1101 2 0.14810562407110694 1101 3 0.13592023381355745 1101 4 0.07401611692620236 1101 5 0.05379967367809968 1101 6 0.06851184758018292 1101 7 0.09093049817465726 1101 8 0.0947997915104537 1101 9 0.08707208560973356 1101 10-11 0.10195621740790983 1101 12-13 0.21828798485388745 1101 14-15 0.37793333544779273 1101 16-17 0.31651534673014936 1101 18-19 0.25852510112667915 1101 20-21 0.2541177042564584 1101 22-23 0.29828470838683074 1101 24-25 0.3464783834914442 1101 26-27 0.43545271556391896 1101 28-29 0.4048551614814002 1101 30-31 0.4822930010026667 1101 32-33 0.5314379357231758 1101 34-35 0.49128461001168944 1101 36-37 0.5592165404955907 1101 38-39 0.6834819064843956 1101 40-41 0.7784747583742444 1101 42-43 0.853673168492179 1101 44-45 0.829613546217189 1101 46-47 0.8701752958037119 1101 48-49 0.8953081864059413 1101 50-51 0.9115457691848405 1101 52-53 1.032297084967368 1101 54-55 1.2018312807132716 1101 56-57 1.257602732063262 1101 58-59 1.4392505534855644 1101 60-61 1.2515643027426009 1101 62-63 1.3548196951267286 1101 64-65 1.4476648712403701 1101 66-67 1.5855974165208302 1101 68-69 1.9287896283479746 1101 70-71 1.9281011268274568 1101 72-73 2.1338658109029858 1101 74-75 2.2407945554614592 1101 76-77 2.349924021030013 1101 78-79 2.3776500684742388 1101 80-81 2.5814462252104313 1101 82-83 2.6302862594321965 1101 84-85 2.9052909603284185 1101 86-87 2.9699483274243654 1101 88-89 3.1857338190881563 1101 90-91 3.279424422694575 1101 92-93 3.4244628141117808 1101 94-95 3.850875446868624 1101 96-97 4.1668022619252305 1101 98-99 4.389841355090272 1101 100-101 4.319972230357621 1102 1 0.17199160287757564 1102 2 0.15238511108167074 1102 3 0.17556931038864576 1102 4 0.09004154679642085 1102 5 -0.1150712444763684 1102 6 0.003051342935613377 1102 7 0.0519594416711584 1102 8 0.15921581584231603 1102 9 0.1299973441906701 1102 10-11 0.20504477726033343 1102 12-13 0.19407759001013147 1102 14-15 0.3251493356234789 1102 16-17 0.39188263047743277 1102 18-19 0.3912941954895075 1102 20-21 0.24758043785771378 1102 22-23 0.28054364068364634 1102 24-25 0.3328026911972657 1102 26-27 0.30464356586147545 1102 28-29 0.35323642287323054 1102 30-31 0.39980925152421776 1102 32-33 0.2958197845494084 1102 34-35 0.43281086887810716 1102 36-37 0.5004881987076004 1102 38-39 0.5388124618569563 1102 40-41 0.619799532733559 1102 42-43 0.6313608180957999 1102 44-45 0.5806573402747688 1102 46-47 0.6422576134794511 1102 48-49 0.6828909487749968 1102 50-51 0.8292315247242925 1102 52-53 0.972159861390935 1102 54-55 1.055669917426279 1102 56-57 1.0523650956357358 1102 58-59 1.2250778752239313 1102 60-61 1.058235937896434 1102 62-63 1.2991623450597487 1102 64-65 1.4330009715543994 1102 66-67 1.455488933309372 1102 68-69 1.7203746306537973 1102 70-71 1.7566286154630681 1102 72-73 1.9583951771326475 1102 74-75 2.1023267984726353 1102 76-77 2.3426709905273455 1102 78-79 2.1853874578499095 1102 80-81 2.1855044635660548 1102 82-83 2.414707444402662 1102 84-85 2.759224986504936 1102 86-87 2.7579799006505326 1102 88-89 2.9852244805283057 1102 90-91 3.2848074079593026 1102 92-93 3.3774454161716427 1102 94-95 3.726315336408998 1102 96-97 3.7713196049649547 1102 98-99 4.220612211756279 1102 100-101 3.6609518183849445 1103 1 0.20272178456912116 1103 2 0.2027966461173989 1103 3 0.21523740353151766 1103 4 0.14441306013363686 1103 5 0.019747499286985715 1103 6 0.12866496209994693 1103 7 0.14876627804161302 1103 8 0.14553168991058385 1103 9 0.2619490717002293 1103 10-11 0.32404202476400457 1103 12-13 0.3346993461161887 1103 14-15 0.4249246488789922 1103 16-17 0.46986902202840497 1103 18-19 0.37130377230156597 1103 20-21 0.37864145487496614 1103 22-23 0.3741506163531838 1103 24-25 0.4187555839972461 1103 26-27 0.4073488478416678 1103 28-29 0.38135675585161977 1103 30-31 0.4158125732716158 1103 32-33 0.47066391021326126 1103 34-35 0.5259075277091938 1103 36-37 0.5801103499408669 1103 38-39 0.4853477319833317 1103 40-41 0.6221374803490605 1103 42-43 0.8153732344661222 1103 44-45 0.819489638910575 1103 46-47 0.7619429850664474 1103 48-49 0.8289621118553896 1103 50-51 0.8692471958713028 1103 52-53 0.9508471014608517 1103 54-55 1.1177969742643228 1103 56-57 1.2228959724833004 1103 58-59 1.3797417157264746 1103 60-61 1.3440917231615401 1103 62-63 1.442697327212052 1103 64-65 1.6590783629111598 1103 66-67 1.8216576727196276 1103 68-69 1.8963706525934008 1103 70-71 1.9024499777813304 1103 72-73 2.131186650421377 1103 74-75 2.2124212941496744 1103 76-77 2.199250193441909 1103 78-79 2.2745870908503996 1103 80-81 2.5721796355133613 1103 82-83 2.680282823289474 1103 84-85 2.995959427533876 1103 86-87 3.1681323259949288 1103 88-89 3.1944798331625996 1103 90-91 3.5617942616082026 1103 92-93 3.4808481981350887 1103 94-95 3.5747679780688024 1103 96-97 3.784999042946726 1103 98-99 4.265970471589256 1103 100-101 3.9850053547734774 1104 1 0.2350673916159849 1104 2 0.19764103874669559 1104 3 0.22371889580782067 1104 4 0.11246254360136732 1104 5 -0.012970529146578258 1104 6 0.011203256733843148 1104 7 0.09720372744087769 1104 8 0.1257026799037888 1104 9 0.19991986090972347 1104 10-11 0.23618620041735738 1104 12-13 0.29067104209079986 1104 14-15 0.3098091982491127 1104 16-17 0.3512026144374829 1104 18-19 0.2790630909323468 1104 20-21 0.24522554114719242 1104 22-23 0.32849337356682184 1104 24-25 0.27482605260049553 1104 26-27 0.3971914593251995 1104 28-29 0.4343377461686586 1104 30-31 0.4473760954064048 1104 32-33 0.5261449814914698 1104 34-35 0.5314015527747742 1104 36-37 0.6431272870178404 1104 38-39 0.7878397168273992 1104 40-41 0.8261126738462607 1104 42-43 0.8834291948275279 1104 44-45 0.764617395638453 1104 46-47 0.8124724711249414 1104 48-49 0.8666479177428812 1104 50-51 0.9914710373169484 1104 52-53 1.2371533289941254 1104 54-55 1.3709350292531468 1104 56-57 1.368507877013883 1104 58-59 1.403508677091807 1104 60-61 1.428765773487008 1104 62-63 1.7307442199223075 1104 64-65 1.7886227134406454 1104 66-67 1.9268472837821626 1104 68-69 2.0004850993047576 1104 70-71 2.1623855326891928 1104 72-73 2.361867695792018 1104 74-75 2.1589950173920123 1104 76-77 2.1901776846171437 1104 78-79 2.1724772427335566 1104 80-81 2.4550790169700143 1104 82-83 2.7011886168708727 1104 84-85 2.77217465471092 1104 86-87 2.92905884563611 1104 88-89 3.2001552112512677 1104 90-91 3.3440070923345893 1104 92-93 3.6056530817129264 1104 94-95 3.7443719160201603 1104 96-97 3.916356923707113 1104 98-99 4.161075559189243 1104 100-101 3.969190399625969 1105 1 0.17033878700853933 1105 2 0.21163316764358342 1105 3 0.12943969601642635 1105 4 0.07862043865736723 1105 5 0.03249085211680125 1105 6 -0.016701562346000287 1105 7 0.11876618385570481 1105 8 0.052651202404348396 1105 9 0.13368859116374665 1105 10-11 0.14465460988451184 1105 12-13 0.2602548074858433 1105 14-15 0.3410125431647373 1105 16-17 0.3222778265267223 1105 18-19 0.2848966819867016 1105 20-21 0.3300857957149219 1105 22-23 0.31153017612826517 1105 24-25 0.2904621383943109 1105 26-27 0.2790045418505045 1105 28-29 0.27696298342360137 1105 30-31 0.41582952673436324 1105 32-33 0.43974173602584443 1105 34-35 0.4466340777737088 1105 36-37 0.5539012383964987 1105 38-39 0.49173918743810674 1105 40-41 0.4122281813747506 1105 42-43 0.4021646032698669 1105 44-45 0.5478443053511981 1105 46-47 0.7381742302982914 1105 48-49 0.7852796313754169 1105 50-51 0.8651953182753047 1105 52-53 0.9925648639805118 1105 54-55 1.1759708979549046 1105 56-57 1.265679919437794 1105 58-59 1.4440424324812042 1105 60-61 1.5177337978886385 1105 62-63 1.5383854965206325 1105 64-65 1.5517601181596703 1105 66-67 1.7839512878571817 1105 68-69 1.957256188464104 1105 70-71 2.110160035894026 1105 72-73 2.331641568338359 1105 74-75 2.3325422288505884 1105 76-77 2.37265962898579 1105 78-79 2.6106245014915253 1105 80-81 2.6301943850259484 1105 82-83 2.7590985297308492 1105 84-85 2.8486065223696606 1105 86-87 3.0730742603850274 1105 88-89 3.031692683887357 1105 90-91 3.204737382740692 1105 92-93 3.480476163813748 1105 94-95 3.61169167806786 1105 96-97 3.8223136144479533 1105 98-99 3.7640660326075945 1105 100-101 3.1250767476888157 1106 1 0.12253041174589185 1106 2 0.12971183108060558 1106 3 0.04847209537049224 1106 4 0.043335911663618276 1106 5 -0.05048982299184246 1106 6 0.029223306254714032 1106 7 0.021009518810096495 1106 8 0.10141896390918959 1106 9 0.10270902741561372 1106 10-11 0.1807499585368575 1106 12-13 0.10136746951378228 1106 14-15 0.018324617247117203 1106 16-17 0.019384450889994298 1106 18-19 0.015499115757101833 1106 20-21 -0.05844712268971364 1106 22-23 0.013491403986783723 1106 24-25 -0.08844389241203743 1106 26-27 -0.1167380940222813 1106 28-29 -0.006662880881307842 1106 30-31 0.10536354636787593 1106 32-33 0.1743438206451131 1106 34-35 0.10783834131423475 1106 36-37 0.2505276825416729 1106 38-39 0.18303152980572435 1106 40-41 0.23915209760341583 1106 42-43 0.18818532866249882 1106 44-45 0.14681520578701424 1106 46-47 0.4234749873639245 1106 48-49 0.5443436296857698 1106 50-51 0.7445771454267884 1106 52-53 0.5382952834606485 1106 54-55 0.6097480156188269 1106 56-57 0.6425041951050758 1106 58-59 0.8785315885330576 1106 60-61 0.8849542743552306 1106 62-63 0.9087898337881555 1106 64-65 0.9024600180904656 1106 66-67 1.1613545270132342 1106 68-69 1.003874585766905 1106 70-71 1.145936725691957 1106 72-73 1.4556940672628507 1106 74-75 1.5716081677553753 1106 76-77 1.6906885695615124 1106 78-79 1.8900951361672185 1106 80-81 1.7291561280382517 1106 82-83 2.079972019340488 1106 84-85 2.3678261033571353 1106 86-87 2.623847497639453 1106 88-89 2.7718205916615837 1106 90-91 2.761129576095602 1106 92-93 2.5837511507205804 1106 94-95 2.8486730366710056 1106 96-97 3.2234866024645896 1106 98-99 3.2614515881943227 1106 100-101 3.1436429367666214 1107 1 0.20987957450373074 1107 2 0.2084270335016356 1107 3 0.21317864692564115 1107 4 0.13905550713977277 1107 5 0.052355161510746484 1107 6 0.1118152485546915 1107 7 0.08827310370855201 1107 8 0.16124669441331463 1107 9 0.20931639075516273 1107 10-11 0.19344333808663094 1107 12-13 0.23144370755658628 1107 14-15 0.35070215745447797 1107 16-17 0.3275784096038663 1107 18-19 0.2862446065509374 1107 20-21 0.16745770187448272 1107 22-23 0.32641175539936995 1107 24-25 0.3883705499356793 1107 26-27 0.474539818027516 1107 28-29 0.4803522680784269 1107 30-31 0.533418617076812 1107 32-33 0.5881746649518007 1107 34-35 0.5528671762079114 1107 36-37 0.6492183182220046 1107 38-39 0.6609544787056265 1107 40-41 0.6868650358747104 1107 42-43 0.6543771218754628 1107 44-45 0.8185124986722059 1107 46-47 0.7504390736211874 1107 48-49 0.9315204951033138 1107 50-51 0.9954239143594066 1107 52-53 1.1416532020976433 1107 54-55 1.1795019862342073 1107 56-57 1.1837980779230008 1107 58-59 1.144594044379751 1107 60-61 1.1624574088422222 1107 62-63 1.4232373128332299 1107 64-65 1.4134046681417018 1107 66-67 1.6842200726367373 1107 68-69 1.8641461763360816 1107 70-71 1.943605987989649 1107 72-73 2.0666002124484706 1107 74-75 2.00257735670289 1107 76-77 2.0727242597532722 1107 78-79 2.064896983286989 1107 80-81 2.1224363925444045 1107 82-83 2.5073820937726197 1107 84-85 2.4719275676558077 1107 86-87 2.6255991110251458 1107 88-89 2.812211718358501 1107 90-91 2.880893630371549 1107 92-93 3.047319799963528 1107 94-95 3.258572148354208 1107 96-97 3.641775836854208 1107 98-99 3.8140587613962076 1107 100-101 3.2982530988689227 1108 1 0.21145879335631435 1108 2 0.1789195775594763 1108 3 0.10012968240211961 1108 4 0.09020011575609743 1108 5 -0.03115352216803302 1108 6 0.03774542956058724 1108 7 0.059743574085146633 1108 8 0.12114723922143611 1108 9 0.09742567636591559 1108 10-11 0.12810163937660235 1108 12-13 0.15943450379494806 1108 14-15 0.3090251885997617 1108 16-17 0.2922941971031179 1108 18-19 0.28155512160349616 1108 20-21 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0.21776567811470215 2316 58-59 0.6137419130958719 2316 60-61 0.6856763199646778 2316 62-63 0.8340655599961551 2316 64-65 0.953070036717186 2316 66-67 1.1109852281948385 2316 68-69 1.3325031381116368 2316 70-71 1.4427171775200307 2316 72-73 1.5681247698752756 2316 74-75 1.8873204479938828 2316 76-77 2.016578964572883 2316 78-79 2.0855715711531957 2316 80-81 2.097240047142087 2316 82-83 2.286392628793287 2316 84-85 2.425721859404316 2316 86-87 2.456633811941966 2316 88-89 2.911807818309242 2316 90-91 3.3815050892409673 2316 92-93 3.3571936504734374 2316 94-95 3.7764752233596646 2316 96-97 4.116972274067166 2316 98-99 4.313904686447678 2316 100-101 4.52872276371501 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 15.0 5 41.0 6 72.0 7 201.0 8 552.0 9 1130.0 10 2057.0 11 3350.0 12 5143.0 13 7903.0 14 10570.0 15 14994.0 16 19660.0 17 26189.0 18 27445.0 19 23559.0 20 22541.0 21 24061.0 22 26336.0 23 28970.0 24 32391.0 25 36272.0 26 40335.0 27 46593.0 28 54999.0 29 66532.0 30 78923.0 31 94182.0 32 113473.0 33 140555.0 34 180887.0 35 244462.0 36 328706.0 37 395138.0 38 203715.0 39 8259.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.74871776225544 18.97607975694079 15.325127184911908 22.95007529589186 2 15.696966128595141 19.86359311021389 40.1850073641018 24.254433397089166 3 19.389588627909827 23.544778752174643 29.960734100470667 27.104898519444866 4 13.04921657518017 17.025751706470977 36.651434067606075 33.27359765074278 5 13.78082684527692 37.09033151386057 34.33147919018655 14.797362450675964 6 32.47303822371511 36.27138599654145 16.91007114056484 14.345504639178605 7 28.622481245284536 32.711271095943715 20.14073124669133 18.525516412080414 8 26.4012133951343 35.0309408833475 19.263859766982424 19.303985954535776 9 25.844173214747173 16.914327169848402 20.172200124750077 37.06929949065435 10-11 25.0175632699757 26.653771785272305 26.88833829173771 21.44032665301428 12-13 26.052313655112354 23.711042113323455 27.465686153563794 22.770958078000398 14-15 22.563731525827535 25.31805974196813 25.969102056796796 26.14910667540754 16-17 22.0394473424381 28.476564343034145 26.90214650645675 22.581841808071008 18-19 21.97592522613657 26.73640464388874 29.474413464697502 21.81325666527719 20-21 22.44661653289147 25.90195994405725 29.917618199833 21.733805323218274 22-23 22.21312974455813 25.857881039081903 29.780204201994405 22.14878501436556 24-25 22.13797770891909 25.999429055367994 29.845319022347137 22.017274213365777 26-27 21.687087298199803 26.37928004287294 29.662605050383494 22.27102760854377 28-29 21.875674990119983 26.785599114196536 29.45521615050348 21.883509745180003 30-31 21.962874515359797 26.311769576823668 29.392646324872228 22.3327095829443 32-33 21.663725387073693 26.321790271831375 29.400394508053136 22.614089833041803 34-35 22.4486876008672 26.447747328101517 29.14927825840012 21.95428681263117 36-37 22.428300704180238 26.01404464713939 29.235296969445123 22.322357679235253 38-39 21.959432769576114 26.442701974163946 29.10708414671179 22.490781109548145 40-41 22.483457175887597 26.208430817542677 29.22967831671962 22.078433689850105 42-43 22.137422424273776 26.247580442508195 28.996704228833487 22.618292904384543 44-45 22.213203990242995 26.523514859521196 29.130743441400607 22.132537708835198 46-47 22.626815899271886 26.80133355322591 28.333634170613266 22.238216376888946 48-49 22.370130277692287 26.843558987264842 28.314393570479684 22.471917164563187 50-51 22.51238951805002 27.020446979269064 28.295780616469695 22.171382886211223 52-53 22.815932169201275 26.72787731379579 28.19888504076886 22.25730547623407 54-55 22.345024432748584 26.964521545359986 28.308485039729998 22.381968982161432 56-57 22.419541177697873 27.125545240516157 28.063811266377776 22.391102315408194 58-59 22.39707577506721 26.832607644556646 28.044159449818395 22.726157130557752 60-61 22.138290785419045 26.994886631960004 28.31653622448781 22.550286358133146 62-63 22.211855239698057 26.981294846938752 28.40100873553546 22.405841177827728 64-65 22.046459664646513 27.090375414625196 28.133869559552576 22.729295361175716 66-67 21.927661403529275 27.09526672579526 28.15124886428516 22.8258230063903 68-69 22.446221635837077 26.799828326514657 28.279981880352757 22.473968157295506 70-71 22.346084938267758 26.862150298305053 27.843377619178096 22.948387144249093 72-73 22.281805318023963 26.948830392270168 27.84238204256826 22.92698224713761 74-75 22.265746234447903 27.39227320007324 27.619286310085755 22.7226942553931 76-77 22.328640704625837 26.973048875452932 28.01619673286385 22.682113687057374 78-79 22.36415681803327 27.153010169165782 27.96133180447395 22.521501208327003 80-81 22.529959332789375 27.206082550758264 27.65673324777131 22.60722486868105 82-83 22.50896249732705 27.297438037349437 27.749566917238553 22.444032548084955 84-85 22.416475821974096 27.385818383381498 27.696126334316613 22.501579460327786 86-87 22.608334298276844 27.32657259639786 27.47599996104153 22.589093144283765 88-89 22.46728556903047 27.563858664481593 27.124008589661848 22.844847176826093 90-91 22.384954354008578 27.448109879359528 27.466398576728118 22.700537189903773 92-93 22.4542348582537 27.11374982198657 27.522152661717353 22.909862658042375 94-95 22.276805792005 26.981446347727207 27.613940496550327 23.127807363717466 96-97 22.481925948113986 26.70386517635998 27.626007588894208 23.188201286631823 98-99 22.193025855990275 26.97605724825222 27.327539077464085 23.503377818293416 100-101 22.659864714124506 26.216280794158475 27.606949817311694 23.516904674405325 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 881.0 1 594.5 2 412.5 3 544.0 4 467.5 5 435.5 6 842.0 7 1982.0 8 3195.0 9 3619.5 10 2993.5 11 2272.0 12 2203.5 13 2315.0 14 2464.5 15 2592.5 16 2814.5 17 3104.0 18 3340.5 19 3646.0 20 4159.0 21 4903.5 22 5974.5 23 7384.0 24 9214.0 25 11734.5 26 14948.5 27 18557.0 28 21759.5 29 24945.5 30 28518.0 31 33208.0 32 39504.5 33 42508.5 34 42866.0 35 45388.0 36 49871.0 37 54454.5 38 57801.0 39 60478.0 40 64032.5 41 68735.0 42 71369.5 43 73441.0 44 77248.5 45 81980.5 46 84053.5 47 84828.5 48 85720.5 49 83968.0 50 82231.5 51 80676.0 52 79323.5 53 79805.0 54 80447.5 55 76437.5 56 70218.5 57 64468.5 58 59747.5 59 53365.5 60 43108.5 61 35252.0 62 29624.5 63 22152.5 64 15365.0 65 11298.0 66 8469.5 67 6473.5 68 4862.0 69 3677.5 70 2746.0 71 1882.5 72 1342.5 73 1057.5 74 778.0 75 560.0 76 395.5 77 234.5 78 139.5 79 88.0 80 54.0 81 32.5 82 23.0 83 17.0 84 12.5 85 9.0 86 5.5 87 2.5 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.015799367938710573 3 0.003116587648184003 4 0.0018180094614406689 5 0.12007519633420036 6 1.7314375823244464E-4 7 0.0 8 3.030015769067781E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.164296977905558E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.8135860712772255E-4 24-25 5.410742444763894E-4 26-27 0.005930173719461229 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0108647708290859 36-37 6.492890933716674E-4 38-39 0.006601105782611952 40-41 0.00993412312858651 42-43 0.005951816689240284 44-45 0.0036360189228813377 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 8.440758213831675E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.7314375823244464E-4 82-83 0.0016015797636501129 84-85 0.004090521288241505 86-87 0.002878514980614392 88-89 0.0 90-91 0.0021210110383474466 92-93 0.0 94-95 0.0 96-97 4.328593955811116E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2310219.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.90177985503709 #Duplication Level Percentage of deduplicated Percentage of total 1 76.12138358561285 42.553208274637626 2 13.786015571847427 15.41325615151056 3 4.495192883578932 7.538678489512765 4 1.9417925711610293 4.341986433487612 5 0.9911108198760663 2.7702429432328586 6 0.6127122144185331 2.0551021994950274 7 0.3981930317947773 1.5581789442240999 8 0.2825595443116768 1.2636465153640764 9 0.21032670144396212 1.0581873265580835 >10 1.0501755099680334 10.636716101554178 >50 0.07006079561844515 2.684496298270165 >100 0.03458881086600436 3.639761190332292 >500 0.00317639920517207 1.2119121235375443 >1k 0.002479140843060675 2.2483800126306246 >5k 2.324194540369383E-4 1.0262469956524178 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9766 0.42273048572451355 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8496 0.3677573424857124 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5434 0.235215795558776 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2735 0.11838704469143402 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2734 0.1183437587518759 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2710 0.11730489620248123 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2687 0.11630931959264468 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2447 0.105920694098698 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 2.1642969779055578E-4 14-15 0.0 0.0 0.0 0.0 2.8135860712772255E-4 16-17 0.0 0.0 0.0 4.328593955811116E-5 3.030015769067781E-4 18-19 0.0 0.0 0.0 4.328593955811116E-5 4.3285939558111155E-4 20-21 0.0 0.0 0.0 6.492890933716674E-5 5.627172142554451E-4 22-23 0.0 0.0 0.0 2.1642969779055578E-4 6.925750329297785E-4 24-25 0.0 0.0 0.0 3.462875164648893E-4 6.925750329297785E-4 26-27 0.0 0.0 0.0 9.306477004993899E-4 8.440758213831675E-4 28-29 0.0 0.0 0.0 0.0033113743761955033 8.657187911622231E-4 30-31 0.0 0.0 0.0 0.0096960504610169 8.873617609412787E-4 32-33 0.0 0.0 0.0 0.023114691724031355 9.522906702784454E-4 34-35 0.0 0.0 0.0 0.043091152830099656 9.955766098365567E-4 36-37 0.0 0.0 0.0 0.07652954113874053 9.955766098365567E-4 38-39 0.0 0.0 0.0 0.12645987241902173 0.0010388625493946678 40-41 0.0 0.0 0.0 0.17749399515803482 0.0010388625493946678 42-43 0.0 0.0 0.0 0.2327484970039637 0.0010388625493946678 44-45 0.0 0.0 0.0 0.2983266954345021 0.0011037914587318344 46-47 0.0 0.0 0.0 0.3760682428808697 0.0011903633378480569 48-49 0.0 4.328593955811116E-5 0.0 0.45582258651668955 0.0012985781867433347 50-51 0.0 4.328593955811116E-5 0.0 0.5437579727289923 0.0013202211565223904 52-53 0.0 4.328593955811116E-5 0.0 0.6274513368646003 0.001385150065859557 54-55 0.0 4.328593955811116E-5 0.0 0.7164255856262978 0.001385150065859557 56-57 0.0 4.328593955811116E-5 0.0 0.8221298500272052 0.001385150065859557 58-59 0.0 4.328593955811116E-5 0.0 0.9313402755323197 0.001385150065859557 60-61 0.0 4.328593955811116E-5 0.0 1.0515669726549735 0.001385150065859557 62-63 0.0 4.328593955811116E-5 0.0 1.1699107314068493 0.0015150078845338904 64-65 0.0 4.328593955811116E-5 0.0 1.2926047270843153 0.0015150078845338904 66-67 0.0 4.328593955811116E-5 0.0 1.4329593861014907 0.0015150078845338904 68-69 4.328593955811116E-5 4.328593955811116E-5 0.0 1.5884641239640052 0.001536650854312946 70-71 4.328593955811116E-5 4.328593955811116E-5 0.0 1.7455920845599486 0.0016665086729872798 72-73 4.328593955811116E-5 4.328593955811116E-5 0.0 1.9063127781392155 0.0018829383707778353 74-75 4.328593955811116E-5 4.328593955811116E-5 0.0 2.0736995064104313 0.0020344391592312244 76-77 4.328593955811116E-5 4.328593955811116E-5 0.0 2.2556519533429515 0.0020777250987893355 78-79 4.328593955811116E-5 4.328593955811116E-5 0.0 2.459507085691876 0.0020777250987893355 80-81 8.657187911622232E-5 4.328593955811116E-5 0.0 2.6730799114715964 0.0020777250987893355 82-83 8.657187911622232E-5 4.328593955811116E-5 0.0 2.893838203217963 0.0021642969779055578 84-85 8.657187911622232E-5 4.328593955811116E-5 0.0 3.1176048677636192 0.0021642969779055578 86-87 8.657187911622232E-5 4.328593955811116E-5 0.0 3.348881642822607 0.002315797766358947 88-89 8.657187911622232E-5 4.328593955811116E-5 0.0 3.59584957097141 0.002467298554812336 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4955 0.0 54.239666 1 GTATCAA 8805 0.0 41.1119 1 ATCAACG 11030 0.0 32.20796 3 TATCAAC 11185 0.0 32.066208 2 TCAACGC 11145 0.0 31.790277 4 CAACGCA 11395 0.0 31.050407 5 AACGCAG 11820 0.0 29.854258 6 ACGCAGA 13165 0.0 26.6599 7 CGCAGAG 13220 0.0 26.405283 8 TGGTATC 2160 0.0 25.986065 2 GTGGTAT 2320 0.0 25.42412 1 TAAGGTG 1440 0.0 25.099195 5 GCAGAGT 14675 0.0 23.528337 9 GGACCGA 575 0.0 23.12731 6 GAGTACT 9605 0.0 22.893835 12-13 AGAGTAC 12955 0.0 21.592993 10-11 GTACTTT 10075 0.0 21.519424 14-15 GTAAGGT 1525 0.0 21.517775 4 CAGAGTA 13920 0.0 21.409643 10-11 AGTACTT 10065 0.0 21.23409 12-13 >>END_MODULE