FastQCFastQC Report
Thu 26 May 2016
SRR938002_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938002_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2305714
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103000.44671628831676435No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84480.3663940974466044No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54380.23584885202588007No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA29030.12590460048384144No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA26420.11458489647892149No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC26000.1127633349149114No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA25910.11237300029405208No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA23920.10374226812171847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45450.053.9927021
GTATCAA83500.042.3107831
ATCAACG104350.033.2939343
TCAACGC105400.032.8720744
TATCAAC106450.032.768222
CAACGCA107900.032.1544845
AACGCAG111900.030.9605986
ACGCAGA123700.027.770667
CGCAGAG124700.027.509868
GTGGTAT21500.025.7587831
TGGTATC20600.025.376282
GCAGAGT137750.024.2806879
GAGTACT91700.022.50982512-13
GTACATG72550.021.7161251
GTGTAAG10850.021.5611631
CAGAGTA131000.021.55894510-11
AGAGTAC122250.021.54763410-11
GTACTTT95800.021.39815514-15
TACATGG71500.021.2025012
ACATGGG71200.020.7596763
Produced by FastQC (version 0.11.5)