##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938002_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2305714 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.758891605810607 33.0 31.0 34.0 30.0 34.0 2 31.96068029252544 33.0 31.0 34.0 30.0 34.0 3 32.040112954165174 34.0 31.0 34.0 30.0 34.0 4 35.56661060304964 37.0 35.0 37.0 33.0 37.0 5 35.571138918356745 37.0 35.0 37.0 33.0 37.0 6 35.589773493156564 37.0 35.0 37.0 33.0 37.0 7 35.528945046957254 37.0 35.0 37.0 33.0 37.0 8 35.49275712425739 37.0 35.0 37.0 33.0 37.0 9 37.11580230679087 39.0 37.0 39.0 34.0 39.0 10-11 37.022507561649014 39.0 37.0 39.0 33.0 39.0 12-13 36.91089788239131 39.0 37.0 39.0 33.0 39.0 14-15 38.06836342234987 40.0 38.0 41.0 33.0 41.0 16-17 37.89903236047489 40.0 38.0 41.0 33.0 41.0 18-19 38.051566889909154 40.0 38.0 41.0 33.0 41.0 20-21 38.01949743116449 40.0 38.0 41.0 33.0 41.0 22-23 37.92094314385912 40.0 38.0 41.0 33.0 41.0 24-25 37.80421531031169 40.0 38.0 41.0 32.5 41.0 26-27 37.61327575753107 40.0 38.0 41.0 32.0 41.0 28-29 37.38845082260853 40.0 37.0 41.0 31.5 41.0 30-31 37.149086139911546 40.0 37.0 41.0 31.0 41.0 32-33 36.87286671287072 39.5 36.0 41.0 30.5 41.0 34-35 36.615646172942526 39.0 36.0 41.0 30.0 41.0 36-37 36.328350784182255 39.0 35.0 41.0 30.0 41.0 38-39 36.00770997617224 39.0 35.0 40.0 29.0 41.0 40-41 35.75232964712883 39.0 35.0 40.0 27.5 41.0 42-43 35.535038604094005 39.0 35.0 40.0 26.5 41.0 44-45 35.228271806477295 38.5 35.0 40.0 25.5 41.0 46-47 34.81881425883696 38.0 34.0 40.0 24.0 41.0 48-49 34.390876752277165 38.0 34.0 40.0 23.0 41.0 50-51 33.68723289185042 37.5 33.0 39.5 22.0 40.5 52-53 33.81851283376863 38.0 33.0 39.5 22.0 40.5 54-55 33.902645557948645 38.0 33.5 40.0 21.0 41.0 56-57 33.57024331725444 38.0 33.0 40.0 18.5 41.0 58-59 33.21383462996712 37.5 33.0 40.0 16.5 41.0 60-61 32.75876648187936 37.0 32.0 40.0 12.0 41.0 62-63 32.17934683139366 36.0 31.0 39.0 8.0 41.0 64-65 31.626131645121642 35.5 31.0 39.0 4.5 40.0 66-67 31.051720421526696 35.0 30.0 38.5 2.0 40.0 68-69 30.41026076954904 35.0 29.0 38.0 2.0 40.0 70-71 29.80576949266041 34.5 28.5 37.5 2.0 39.5 72-73 29.110375354445523 34.0 27.5 36.5 2.0 39.0 74-75 28.376536942569636 34.0 26.0 36.0 2.0 39.0 76-77 27.66121817363298 33.5 25.5 35.5 2.0 38.0 78-79 26.88800128723684 33.0 24.5 35.0 2.0 37.0 80-81 26.175528491391386 32.0 22.0 35.0 2.0 36.5 82-83 25.387563245051204 32.0 19.0 35.0 2.0 36.0 84-85 24.673825548181604 31.0 16.0 34.5 2.0 35.5 86-87 23.930279731137514 31.0 7.5 34.0 2.0 35.0 88-89 23.05274526675902 30.0 2.0 34.0 2.0 35.0 90-91 22.16104295675873 29.5 2.0 34.0 2.0 35.0 92-93 21.208073074110665 29.0 2.0 34.0 2.0 35.0 94-95 20.204197051325533 28.0 2.0 34.0 2.0 35.0 96-97 18.950033482036368 25.5 2.0 33.0 2.0 35.0 98-99 17.422598162651568 21.5 2.0 32.5 2.0 34.5 100-101 13.969318614537624 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.38501141692285046 1101 2 0.29335697221273804 1101 3 0.333325754155382 1101 4 0.24280297875218082 1101 5 0.2737037134160829 1101 6 0.27451387710824093 1101 7 0.21009977234124477 1101 8 0.2505554568107158 1101 9 0.341316543029734 1101 10-11 0.3852237390273956 1101 12-13 0.4027169389823584 1101 14-15 0.5172719895074209 1101 16-17 0.7085458323064913 1101 18-19 0.6010094401787356 1101 20-21 0.6157932285592622 1101 22-23 0.6584091837885424 1101 24-25 0.7230325920756826 1101 26-27 0.7195965072308681 1101 28-29 0.8944262239314611 1101 30-31 0.8083849709999384 1101 32-33 0.733470383476714 1101 34-35 0.8983914332073084 1101 36-37 0.8948095803800413 1101 38-39 0.9675706869036489 1101 40-41 1.1169842759921025 1101 42-43 1.2929978140436518 1101 44-45 1.219800839222927 1101 46-47 1.3373416441364299 1101 48-49 1.4516184646963453 1101 50-51 1.3344825300013454 1101 52-53 1.202754147403688 1101 54-55 1.376466740862405 1101 56-57 1.448798652080221 1101 58-59 1.4483012960880473 1101 60-61 1.6266789688815066 1101 62-63 1.6063903174551797 1101 64-65 1.651977407525056 1101 66-67 1.618408940409438 1101 68-69 1.9261903013091661 1101 70-71 2.2555054532232717 1101 72-73 2.23546358435253 1101 74-75 2.517861555065565 1101 76-77 2.709395529007434 1101 78-79 2.846950903800984 1101 80-81 3.1607078645687956 1101 82-83 3.1937163596328197 1101 84-85 3.2747870019605507 1101 86-87 3.257527716756126 1101 88-89 3.6781361059740227 1101 90-91 4.105221630219329 1101 92-93 4.309427171679179 1101 94-95 4.445168579888897 1101 96-97 4.493884063711803 1101 98-99 4.7370960708249505 1101 100-101 4.541984566079718 1102 1 0.44038162283028726 1102 2 0.411761173445921 1102 3 0.34712552573765265 1102 4 0.29672458747423747 1102 5 0.33010838560478106 1102 6 0.39619065956080135 1102 7 0.238950533252968 1102 8 0.3560701565206088 1102 9 0.3836351681151342 1102 10-11 0.5085576908306564 1102 12-13 0.5511558548892381 1102 14-15 0.6543632020542915 1102 16-17 0.6230020788598836 1102 18-19 0.5686867814066758 1102 20-21 0.6972020226849764 1102 22-23 0.7105605120423846 1102 24-25 0.6278077753888809 1102 26-27 0.5786686645902748 1102 28-29 0.6261183662495711 1102 30-31 0.7484804191330525 1102 32-33 0.760583258255501 1102 34-35 0.8702641099092574 1102 36-37 0.9524566452810674 1102 38-39 0.8773731266598688 1102 40-41 0.9732661080004661 1102 42-43 1.036848379082656 1102 44-45 0.9871539788044075 1102 46-47 1.0146486481576318 1102 48-49 1.1797132214633095 1102 50-51 1.3861538624567586 1102 52-53 1.3476146192976728 1102 54-55 1.319051386937609 1102 56-57 1.5152146805820692 1102 58-59 1.5648590202105694 1102 60-61 1.654635818335457 1102 62-63 1.7169420672443465 1102 64-65 1.673300628946901 1102 66-67 1.7458835555195336 1102 68-69 1.8130024490342258 1102 70-71 2.0295942468496193 1102 72-73 2.3612334610215946 1102 74-75 2.428164008382211 1102 76-77 2.6306399493253316 1102 78-79 3.006300683842369 1102 80-81 3.1828545047919263 1102 82-83 3.4761759140675714 1102 84-85 3.5189346047582184 1102 86-87 3.5473303459277297 1102 88-89 4.054885776757171 1102 90-91 4.185577339392669 1102 92-93 4.405480465418957 1102 94-95 4.909673536329816 1102 96-97 5.027733991626334 1102 98-99 4.981349574040923 1102 100-101 4.843494680215402 1103 1 0.6473446215304648 1103 2 0.676849844771116 1103 3 0.5566861058607202 1103 4 0.445007766296861 1103 5 0.4216348244593817 1103 6 0.40483183666170675 1103 7 0.4685864629080996 1103 8 0.4135469514426475 1103 9 0.5844466405420974 1103 10-11 0.5923315035035159 1103 12-13 0.7223650968541122 1103 14-15 0.8281154130785424 1103 16-17 0.873766263709534 1103 18-19 0.8300046113857036 1103 20-21 0.8742783407453132 1103 22-23 0.9184213794109581 1103 24-25 1.0972085457596776 1103 26-27 0.9667848848763683 1103 28-29 1.0140127723388161 1103 30-31 1.1121714539571101 1103 32-33 1.1902458987917441 1103 34-35 1.1915127970125141 1103 36-37 1.1914728307291256 1103 38-39 1.324227402756172 1103 40-41 1.476422624963682 1103 42-43 1.478247371833966 1103 44-45 1.526766707263981 1103 46-47 1.7780258301286125 1103 48-49 1.7616945321289776 1103 50-51 1.5621629099912653 1103 52-53 1.7220543249189149 1103 54-55 1.7250105793372228 1103 56-57 1.8904517008279882 1103 58-59 1.8758157356418508 1103 60-61 2.006438181627253 1103 62-63 2.115350259202401 1103 64-65 2.2010979337361753 1103 66-67 2.3698818327712345 1103 68-69 2.3675705532397906 1103 70-71 2.5235163566779306 1103 72-73 2.733739992504969 1103 74-75 2.73778919156657 1103 76-77 2.9691804377928044 1103 78-79 3.341308192395285 1103 80-81 3.3518958171037276 1103 82-83 3.4678211283421554 1103 84-85 3.5099744241595303 1103 86-87 3.595519027243622 1103 88-89 4.106928770922707 1103 90-91 4.345850520270371 1103 92-93 4.700467335157338 1103 94-95 5.2854716921401845 1103 96-97 5.576077192283549 1103 98-99 5.5251463601421555 1103 100-101 5.017192149462945 1104 1 0.32207862188568015 1104 2 0.2888370568959182 1104 3 0.32326736215105 1104 4 0.3555570528991936 1104 5 0.27451529334813785 1104 6 0.2803323990914066 1104 7 0.34141009679596834 1104 8 0.2809094521826836 1104 9 0.41399184212874474 1104 10-11 0.4336280519612785 1104 12-13 0.42024704582100725 1104 14-15 0.5512643089402403 1104 16-17 0.5914556841109331 1104 18-19 0.5836476165363749 1104 20-21 0.5324622256544345 1104 22-23 0.46540450652006626 1104 24-25 0.5916536348603785 1104 26-27 0.7211787040700202 1104 28-29 0.6863863440634077 1104 30-31 0.6918447711082507 1104 32-33 0.7738752872234542 1104 34-35 0.7257706858290405 1104 36-37 0.7621695331150136 1104 38-39 0.8888839654226786 1104 40-41 0.9067406641561533 1104 42-43 0.9076117670740373 1104 44-45 0.8303708751640428 1104 46-47 0.7809420577054311 1104 48-49 0.982397746858716 1104 50-51 1.0711055421622362 1104 52-53 1.0545350543014251 1104 54-55 1.1316731807225793 1104 56-57 1.2568566502093077 1104 58-59 1.2122994251806603 1104 60-61 1.3707307634755708 1104 62-63 1.4365709092527226 1104 64-65 1.6178298027788607 1104 66-67 1.5959795179290097 1104 68-69 1.7734581857067901 1104 70-71 1.8540077426231072 1104 72-73 1.9534498971879408 1104 74-75 2.1280230439139736 1104 76-77 2.163197606699324 1104 78-79 2.531995067062269 1104 80-81 2.677051225309281 1104 82-83 2.8284345911329893 1104 84-85 2.8731961398661205 1104 86-87 3.1253489958659664 1104 88-89 3.4294498768371007 1104 90-91 3.462554208625118 1104 92-93 3.817205124512615 1104 94-95 3.821162770229108 1104 96-97 4.022789287482173 1104 98-99 4.354471089287852 1104 100-101 4.053756118440505 1105 1 0.43423248846971774 1105 2 0.30531370688927595 1105 3 0.17937714038131958 1105 4 0.18364399017509925 1105 5 0.19351825913096832 1105 6 0.24431894240511554 1105 7 0.3533323667264767 1105 8 0.3034573694948861 1105 9 0.44608657011776387 1105 10-11 0.42083136301435076 1105 12-13 0.3258079328999415 1105 14-15 0.3639415697963315 1105 16-17 0.4907123792550081 1105 18-19 0.38643040614285695 1105 20-21 0.4779080226532315 1105 22-23 0.5147165334334431 1105 24-25 0.47989134014124346 1105 26-27 0.51024665148271 1105 28-29 0.5619690097267238 1105 30-31 0.7229119217434601 1105 32-33 0.6279484136897437 1105 34-35 0.5858963996088704 1105 36-37 0.5315583162587387 1105 38-39 0.7388390802914344 1105 40-41 0.8625459848590893 1105 42-43 0.8420239666225271 1105 44-45 1.1016944996162579 1105 46-47 1.1882565634820708 1105 48-49 1.3271258244117234 1105 50-51 1.3756051216407954 1105 52-53 1.2592257718438091 1105 54-55 1.2773047154045258 1105 56-57 1.3468196443220108 1105 58-59 1.3231623351890676 1105 60-61 1.3133310910396503 1105 62-63 1.4454200969823532 1105 64-65 1.529160952351699 1105 66-67 1.7307249475248696 1105 68-69 1.8068255443894436 1105 70-71 1.9784453505200545 1105 72-73 2.1389720077629484 1105 74-75 2.302265441328874 1105 76-77 2.421367054709595 1105 78-79 2.6489645681910368 1105 80-81 2.6191653415502607 1105 82-83 2.91326828596722 1105 84-85 3.1056215305084116 1105 86-87 3.377951448169984 1105 88-89 3.4039860195487996 1105 90-91 3.5773724105639424 1105 92-93 3.6188554277307112 1105 94-95 3.8362370033307833 1105 96-97 4.097853959254117 1105 98-99 4.445982133509002 1105 100-101 4.351651209405935 1106 1 0.23910364345599788 1106 2 0.15656872212456463 1106 3 0.1026237038249107 1106 4 0.19126714780859544 1106 5 0.23124868420232048 1106 6 0.011397972861892924 1106 7 0.04288895788311464 1106 8 0.11591376624653549 1106 9 0.06793748492425777 1106 10-11 0.1391629958784506 1106 12-13 0.26868233486329274 1106 14-15 0.3384260863345432 1106 16-17 0.2474129748788343 1106 18-19 0.24186288832239455 1106 20-21 0.2296011385103398 1106 22-23 0.12697008029056178 1106 24-25 0.2266812448690061 1106 26-27 0.16492633133490386 1106 28-29 0.1487241352627109 1106 30-31 0.24663295057330714 1106 32-33 0.37012981699049163 1106 34-35 0.30372581956502387 1106 36-37 0.42301983509948116 1106 38-39 0.12338698526737346 1106 40-41 0.573998645174278 1106 42-43 0.4677338550809367 1106 44-45 0.5947824368551551 1106 46-47 0.8215982268167323 1106 48-49 0.8183429582478468 1106 50-51 0.6493842306868274 1106 52-53 0.7699099464498289 1106 54-55 0.4232396173058319 1106 56-57 0.7625369780781668 1106 58-59 0.9950289333773839 1106 60-61 1.0036177350027486 1106 62-63 0.8071062845762782 1106 64-65 1.067413927023896 1106 66-67 1.2166900442447997 1106 68-69 1.0461484359224862 1106 70-71 1.351455973856929 1106 72-73 1.5251237539610223 1106 74-75 1.7041360723867953 1106 76-77 1.8985461830582544 1106 78-79 2.1261857486843603 1106 80-81 2.2430456170262794 1106 82-83 2.143768499674838 1106 84-85 2.3343290130326437 1106 86-87 2.3699201779194645 1106 88-89 2.6927082892616028 1106 90-91 2.9994325520245972 1106 92-93 3.2779411038051087 1106 94-95 3.6210294656562 1106 96-97 3.635527855212974 1106 98-99 4.077539682903041 1106 100-101 3.990114617146288 1107 1 0.2399931590324087 1107 2 0.15626826352986356 1107 3 0.126681476214344 1107 4 0.15587233467275752 1107 5 0.2418200826525876 1107 6 0.24076648269269185 1107 7 0.1316942396289349 1107 8 0.16970112590074393 1107 9 0.23503990440662648 1107 10-11 0.1786508984666071 1107 12-13 0.21102788860320487 1107 14-15 0.3444392116310482 1107 16-17 0.36796473809637575 1107 18-19 0.2610289839420261 1107 20-21 0.478539649922503 1107 22-23 0.3508321592531871 1107 24-25 0.3499476778456554 1107 26-27 0.35791563079197175 1107 28-29 0.4423242354155832 1107 30-31 0.5297571928378133 1107 32-33 0.6045165124730758 1107 34-35 0.5307440315728229 1107 36-37 0.4611965258379769 1107 38-39 0.4529030887938106 1107 40-41 0.5355952926889032 1107 42-43 0.7658164548384647 1107 44-45 0.6991351330756999 1107 46-47 0.837896787778206 1107 48-49 0.899367284851607 1107 50-51 0.7001650276927833 1107 52-53 0.7161456483105653 1107 54-55 0.9013318370095931 1107 56-57 0.8658888045501456 1107 58-59 0.89154862132213 1107 60-61 1.0931188155994462 1107 62-63 1.1800597398501225 1107 64-65 1.2331082762938372 1107 66-67 1.5117061214152159 1107 68-69 1.4822304822036543 1107 70-71 1.6431459216720157 1107 72-73 1.8670871454532971 1107 74-75 2.0051494612434695 1107 76-77 2.1545889588739726 1107 78-79 2.3220999071367316 1107 80-81 2.313941985167123 1107 82-83 2.5582043491292694 1107 84-85 2.558881619642733 1107 86-87 2.8073312263618213 1107 88-89 3.1308171132561213 1107 90-91 3.083569524245359 1107 92-93 3.4286948858935595 1107 94-95 3.5246270619874416 1107 96-97 3.677790387499094 1107 98-99 3.943337479540009 1107 100-101 3.8383329503917683 1108 1 0.27580053363811885 1108 2 0.23152585496081102 1108 3 0.1488786725188902 1108 4 0.14219463735160787 1108 5 0.11299506647350199 1108 6 0.13725791414687194 1108 7 0.14665607227349398 1108 8 0.20156755544400795 1108 9 0.24704363960977105 1108 10-11 0.281270891230605 1108 12-13 0.2885308059344496 1108 14-15 0.31650894986108824 1108 16-17 0.467701159129696 1108 18-19 0.4917437046171145 1108 20-21 0.41819197792734997 1108 22-23 0.39631258892893584 1108 24-25 0.31486549573284606 1108 26-27 0.395522769463291 1108 28-29 0.3858318357641295 1108 30-31 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-2.69141204579471 2316 78-79 -3.53579400207483 2316 80-81 -3.5811024216829104 2316 82-83 -3.419036908523143 2316 84-85 -3.2388816949977475 2316 86-87 -3.058763306411631 2316 88-89 -3.4983035112449414 2316 90-91 -3.5995064734413056 2316 92-93 -3.9982389468494937 2316 94-95 -4.309357153716036 2316 96-97 -4.36334186669073 2316 98-99 -4.191300017957584 2316 100-101 -3.6968129984331544 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7652.0 3 2498.0 4 2672.0 5 2883.0 6 3388.0 7 4076.0 8 5117.0 9 6359.0 10 7993.0 11 10094.0 12 12196.0 13 14728.0 14 17350.0 15 21022.0 16 24682.0 17 29342.0 18 29224.0 19 25343.0 20 25601.0 21 27425.0 22 29918.0 23 32943.0 24 37081.0 25 40832.0 26 46055.0 27 52585.0 28 60534.0 29 70566.0 30 81223.0 31 97105.0 32 116919.0 33 144608.0 34 184740.0 35 242695.0 36 304406.0 37 318734.0 38 156666.0 39 8459.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.462157150475605 18.561452367922982 14.963384071635467 23.013006409965946 2 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22.179437545614157 26.517491435654357 29.135692418805448 22.167378599926042 46-47 22.589907364948612 26.799307785847436 28.410562469550776 22.200222379653173 48-49 22.32027409116199 26.852539345291742 28.317439039336072 22.509747524210194 50-51 22.488363921868018 27.09907041508157 28.272503285863632 22.14006237718678 52-53 22.742183750216615 26.774232798047493 28.253102631432263 22.23048082030363 54-55 22.29933341401596 26.98107298350908 28.38475549476886 22.3348381077061 56-57 22.400867026510717 27.15658638458664 28.06616607756608 22.376380511336563 58-59 22.37162151757595 26.758706426119243 28.123275094935522 22.746396961369282 60-61 22.083605169278606 26.951427832761897 28.44068395080579 22.524283047153702 62-63 22.166498541311014 26.92489795900662 28.56048185861984 22.348121641062523 64-65 21.975057958720853 27.063105980142755 28.323199820240667 22.63863624089573 66-67 21.88620083830281 27.056616777810717 28.236885713957307 22.820296669929167 68-69 22.416940117072198 26.744859385403497 28.35853848439435 22.479662013129957 70-71 22.359378046667107 26.78401438780576 27.981501640469258 22.875105925057873 72-73 22.261332199724766 26.94100283149104 27.983112991783145 22.81455197700105 74-75 22.3261984533623 27.28770601738189 27.811826035357175 22.57426949389864 76-77 22.512108076127983 26.958394021911214 27.8960271283365 22.633470773624303 78-79 22.563663237387992 27.159323195507923 27.741892107309273 22.53512145979481 80-81 22.723377582480506 27.13335581758261 27.489987821634116 22.65327877830277 82-83 22.682688097206068 27.229636190798836 27.592224330102255 22.495451381892845 84-85 22.64908021843389 27.36212168630199 27.509557404393963 22.479240690870157 86-87 22.887835475520426 27.30290443833813 27.226786298648108 22.582473787493335 88-89 22.799061271777614 27.384894839923152 26.98411975357408 22.831924134725156 90-91 22.711417618669966 27.425807951881243 27.046843247877188 22.8159311815716 92-93 22.889106260966503 26.997525283963363 27.247881525951982 22.86548692911815 94-95 22.748992921709288 26.807263545392818 27.336953102798994 23.106790430098897 96-97 23.031359723948185 26.488167736010265 27.2913304971271 23.189142042914447 98-99 22.819536412349382 26.77487211255483 26.898703426064746 23.50688804903104 100-101 23.70140270855427 25.663613605015495 27.125525768664176 23.509457917766056 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 917.0 1 619.0 2 423.0 3 534.5 4 443.5 5 425.5 6 853.5 7 1928.5 8 3183.0 9 3697.0 10 3054.0 11 2249.0 12 2067.0 13 2157.5 14 2366.5 15 2604.5 16 2826.0 17 3042.5 18 3317.0 19 3766.5 20 4284.5 21 5058.0 22 6186.0 23 7586.5 24 9482.0 25 12004.0 26 15090.5 27 18785.0 28 21946.5 29 24844.0 30 28675.5 31 33378.5 32 39522.0 33 42580.5 34 43299.5 35 45868.5 36 49787.5 37 54178.5 38 57525.5 39 60404.0 40 64149.5 41 68362.0 42 70709.5 43 72875.0 44 75824.0 45 80306.5 46 82947.0 47 83968.0 48 84823.5 49 83119.5 50 81207.0 51 79492.5 52 78370.5 53 78703.0 54 79177.0 55 75631.5 56 69846.5 57 64306.5 58 59957.0 59 53631.5 60 43764.0 61 35966.0 62 30072.5 63 22921.5 64 16308.5 65 12329.5 66 9241.0 67 6888.5 68 5307.0 69 4018.5 70 3045.5 71 2258.5 72 1625.0 73 1227.0 74 903.0 75 620.5 76 445.5 77 295.0 78 186.5 79 118.5 80 79.5 81 51.5 82 37.5 83 26.5 84 15.5 85 10.0 86 8.5 87 5.0 88 6.5 89 7.5 90 4.0 91 2.5 92 2.5 93 1.0 94 0.5 95 0.5 96 0.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.45703847051282165 2 0.04037794800222404 3 0.05312887895029479 4 0.05742255977974718 5 0.08253408705502938 6 0.0811896011387362 7 0.04770756477169328 8 0.04115861724394266 9 0.04688352501654585 10-11 0.05048327763113725 12-13 0.04590768846439758 14-15 0.039380426193361365 16-17 0.04011772492165117 18-19 0.04788104682540853 20-21 0.055644368729165894 22-23 0.04759913848812125 24-25 0.0642967861582139 26-27 0.05649009374102772 28-29 0.04469331408839084 30-31 0.04716543335383313 32-33 0.04536555704653743 34-35 0.050374851347565225 36-37 0.04530050127639421 38-39 0.027063200379578736 40-41 0.024742877911137287 42-43 0.02417906123656273 44-45 0.016112145738803686 46-47 0.02806072218844141 48-49 0.027822184364582947 50-51 0.016654277156663837 52-53 0.016155516252232497 54-55 0.016697647670092648 56-57 0.01576518163137319 58-59 0.014464066228508826 60-61 0.017434946398382452 62-63 0.016936185493951116 64-65 0.017760225249098545 66-67 0.009649939237910687 68-69 0.012967783515214808 70-71 0.01858426500424597 72-73 0.016979556007379926 74-75 0.014138787377792734 76-77 0.009888477061769152 78-79 0.01394362006736308 80-81 0.017196408574523987 82-83 0.014897771362796948 84-85 0.013119580312215653 86-87 0.003794919925021056 88-89 0.029513634388306616 90-91 0.03252788507160905 92-93 0.017304834858096016 94-95 0.0337856299610446 96-97 0.02773544333772532 98-99 0.024374228546992383 100-101 0.15134140660983972 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2305714.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.686745119171725 #Duplication Level Percentage of deduplicated Percentage of total 1 77.46807681797014 44.6888120227066 2 13.118560602613453 15.135341236267402 3 4.1394086015454326 7.163670268243752 4 1.8548298561869025 4.279963886131352 5 0.9499899511620865 2.7400914089230843 6 0.5597515322247446 1.9374146381708812 7 0.3715828637438149 1.5004784166008955 8 0.26755374653188824 1.234744382549161 9 0.18287044496599242 0.9494280673724453 >10 0.987223360965081 10.118599791075766 >50 0.06348604293430783 2.506123115510043 >100 0.03085045994491625 3.2776092536165127 >500 0.003247739299832824 1.2465410879759828 >1k 0.0023413934487160047 2.1673955407672545 >5k 1.5105764185264546E-4 0.6050146643701697 >10k+ 7.552882092632273E-5 0.4487722197186193 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10300 0.44671628831676435 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8448 0.3663940974466044 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5438 0.23584885202588007 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2903 0.12590460048384144 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2642 0.11458489647892149 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2600 0.1127633349149114 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2591 0.11237300029405208 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2392 0.10374226812171847 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.9516731042965434E-4 14-15 4.337051342881207E-5 0.0 0.0 0.0 3.0359359400168454E-4 16-17 4.337051342881207E-5 0.0 0.0 0.0 4.1201987757371467E-4 18-19 4.337051342881207E-5 0.0 0.0 2.1685256714406036E-5 4.337051342881207E-4 20-21 4.337051342881207E-5 0.0 0.0 1.0842628357203018E-4 5.204461611457449E-4 22-23 4.337051342881207E-5 0.0 0.0 2.1685256714406036E-4 6.07187188003369E-4 24-25 4.337051342881207E-5 0.0 0.0 5.204461611457449E-4 6.505577014321811E-4 26-27 4.337051342881207E-5 0.0 0.0 0.0012577448894355503 6.939282148609932E-4 28-29 4.337051342881207E-5 0.0 0.0 0.002970880169873627 7.372987282898053E-4 30-31 4.337051342881207E-5 0.0 0.0 0.009042752049907317 7.372987282898053E-4 32-33 4.337051342881207E-5 0.0 0.0 0.023224909941128864 7.372987282898053E-4 34-35 4.337051342881207E-5 0.0 0.0 0.044216238440673905 8.023544984330234E-4 36-37 4.337051342881207E-5 0.0 0.0 0.07767658955100243 8.240397551474294E-4 38-39 1.0842628357203018E-4 0.0 0.0 0.12865862808657103 8.240397551474294E-4 40-41 1.3011154028643622E-4 0.0 0.0 0.17966235187885402 8.240397551474294E-4 42-43 1.3011154028643622E-4 0.0 0.0 0.2332249359634369 8.240397551474294E-4 44-45 1.3011154028643622E-4 0.0 0.0 0.2985192439305135 8.890955252906475E-4 46-47 1.3011154028643622E-4 0.0 0.0 0.3782559328693845 9.758365521482717E-4 48-49 1.3011154028643622E-4 0.0 0.0 0.4630236013659977 0.0010408923222914898 50-51 1.3011154028643622E-4 0.0 0.0 0.5532776398113557 0.0010408923222914898 52-53 1.3011154028643622E-4 0.0 0.0 0.6387392365228298 0.001214374376006738 54-55 1.3011154028643622E-4 0.0 0.0 0.7311184301261995 0.001236059632721144 56-57 1.734820537152483E-4 0.0 0.0 0.8387423591997967 0.001279430146149956 58-59 1.734820537152483E-4 0.0 0.0 0.9523947896399987 0.0013011154028643621 60-61 1.734820537152483E-4 0.0 0.0 1.07081797655737 0.0013011154028643621 62-63 1.734820537152483E-4 0.0 0.0 1.1867907294660136 0.0013444859162931742 64-65 1.734820537152483E-4 0.0 0.0 1.3044332471416662 0.0014095416864363924 66-67 1.734820537152483E-4 0.0 0.0 1.4449103401375885 0.0014312269431507984 68-69 1.734820537152483E-4 0.0 0.0 1.5997647583351622 0.0014312269431507984 70-71 1.951673104296543E-4 0.0 0.0 1.7609729567500567 0.0014529121998652045 72-73 2.1685256714406036E-4 0.0 0.0 1.9226365455559535 0.0015179679700084226 74-75 2.1685256714406036E-4 0.0 0.0 2.087466181842154 0.0016047089968660468 76-77 2.1685256714406036E-4 0.0 0.0 2.267692350395582 0.0016480795102948589 78-79 2.1685256714406036E-4 0.0 0.0 2.4639872941743857 0.0016480795102948589 80-81 2.1685256714406036E-4 0.0 0.0 2.6741174317369802 0.0016480795102948589 82-83 2.1685256714406036E-4 0.0 0.0 2.8882810270484542 0.001821561564010107 84-85 2.1685256714406036E-4 0.0 0.0 3.1030301242912177 0.0018649320774389191 86-87 2.1685256714406036E-4 0.0 0.0 3.326670176786887 0.0020167288744397617 88-89 2.1685256714406036E-4 0.0 0.0 3.5687426974898013 0.0021251551580117917 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4545 0.0 53.992702 1 GTATCAA 8350 0.0 42.310783 1 ATCAACG 10435 0.0 33.293934 3 TCAACGC 10540 0.0 32.872074 4 TATCAAC 10645 0.0 32.76822 2 CAACGCA 10790 0.0 32.154484 5 AACGCAG 11190 0.0 30.960598 6 ACGCAGA 12370 0.0 27.77066 7 CGCAGAG 12470 0.0 27.50986 8 GTGGTAT 2150 0.0 25.758783 1 TGGTATC 2060 0.0 25.37628 2 GCAGAGT 13775 0.0 24.280687 9 GAGTACT 9170 0.0 22.509825 12-13 GTACATG 7255 0.0 21.716125 1 GTGTAAG 1085 0.0 21.561163 1 CAGAGTA 13100 0.0 21.558945 10-11 AGAGTAC 12225 0.0 21.547634 10-11 GTACTTT 9580 0.0 21.398155 14-15 TACATGG 7150 0.0 21.202501 2 ACATGGG 7120 0.0 20.759676 3 >>END_MODULE