FastQCFastQC Report
Thu 26 May 2016
SRR938002_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938002_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2305714
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101530.44034082284272896No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88150.3823110758749784No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54780.23758367256303253No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28590.12399629789297373No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC28260.12256507094982293No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA26710.11584264136835704No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26370.11436804391177742No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA24570.10656135149459127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48750.050.671741
GTATCAA84400.039.793681
ATCAACG107450.030.989923
TCAACGC108800.030.8196834
TATCAAC109850.030.5310522
CAACGCA110600.030.3610425
AACGCAG114700.029.2757726
ACGCAGA127650.026.0453137
CGCAGAG129300.025.6394848
TGGTATC23200.024.1619052
GTGGTAT24700.024.040871
TAAGGTG15500.023.9009555
GCAGAGT142550.022.8897959
GAGTACT94750.022.13114412-13
GTACATG70700.021.5014481
AGAGTAC123700.021.44400410-11
GTACTTT97200.021.37785714-15
GTAAGGT15350.021.3497644
TACATGG68450.021.2366072
GTGTAAG10250.020.855751
Produced by FastQC (version 0.11.5)