##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938002_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2305714 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.491997272862115 33.0 31.0 34.0 31.0 34.0 2 32.723783175189986 34.0 31.0 34.0 31.0 34.0 3 32.68792703691785 34.0 31.0 34.0 31.0 34.0 4 36.21307065837307 37.0 35.0 37.0 35.0 37.0 5 36.17442189274125 37.0 35.0 37.0 35.0 37.0 6 36.234628405778 37.0 37.0 37.0 35.0 37.0 7 36.198977410034374 37.0 37.0 37.0 35.0 37.0 8 36.19336353077615 37.0 37.0 37.0 35.0 37.0 9 37.90463171061111 39.0 38.0 39.0 35.0 39.0 10-11 37.86679635895865 39.0 38.0 39.0 35.0 39.0 12-13 37.80802801214722 39.0 38.0 39.0 35.0 39.0 14-15 39.08780121905839 40.0 38.5 41.0 36.0 41.0 16-17 39.01832989694299 40.0 38.0 41.0 36.0 41.0 18-19 39.01335009459109 40.0 38.5 41.0 36.0 41.0 20-21 39.01702878154012 40.0 38.5 41.0 35.5 41.0 22-23 39.046452855818195 40.0 39.0 41.0 35.5 41.0 24-25 38.99074299761375 40.0 38.5 41.0 35.0 41.0 26-27 38.81711326730028 40.0 38.0 41.0 35.0 41.0 28-29 38.735172705721524 40.0 38.0 41.0 35.0 41.0 30-31 38.509989747210625 40.0 38.0 41.0 34.0 41.0 32-33 38.3006472181719 40.0 38.0 41.0 34.0 41.0 34-35 38.0876058348954 40.0 38.0 41.0 34.0 41.0 36-37 37.9976688349032 40.0 38.0 41.0 33.0 41.0 38-39 37.93624252617627 40.0 38.0 41.0 33.0 41.0 40-41 37.80432048380675 40.0 37.5 41.0 33.0 41.0 42-43 37.614142950947084 40.0 37.0 41.0 33.0 41.0 44-45 37.35363492610098 40.0 37.0 41.0 32.5 41.0 46-47 36.993003685626235 40.0 36.5 41.0 31.0 41.0 48-49 36.60648718791663 39.5 36.0 41.0 30.5 41.0 50-51 36.29150102744747 39.0 35.5 41.0 30.0 41.0 52-53 35.81501283333492 39.0 35.0 40.0 28.5 41.0 54-55 35.49519671563776 38.5 35.0 40.0 28.0 41.0 56-57 35.19849166028398 38.0 35.0 40.0 27.0 41.0 58-59 34.801576865127245 38.0 34.0 40.0 26.5 41.0 60-61 34.37232805109393 37.5 34.0 40.0 26.0 41.0 62-63 33.92786204186642 37.0 33.0 40.0 25.0 41.0 64-65 33.47620671948039 36.0 33.0 39.0 23.5 40.5 66-67 32.943168797170856 36.0 32.0 39.0 22.0 40.0 68-69 32.3938591256331 35.0 31.5 38.5 20.5 40.0 70-71 31.78585678882984 35.0 31.0 37.5 18.5 40.0 72-73 31.294642787440246 35.0 30.5 37.0 17.5 39.0 74-75 30.73408345527676 34.0 30.0 36.5 13.0 39.0 76-77 29.534642197601265 33.0 28.5 35.0 12.5 37.5 78-79 30.22952369634742 34.0 30.0 36.0 9.5 37.5 80-81 30.026588726962668 34.0 30.0 35.0 7.0 37.0 82-83 29.545080395920742 34.0 29.0 35.0 4.5 37.0 84-85 29.011960069635695 34.0 29.0 35.0 2.0 36.0 86-87 28.444415699431932 34.0 28.5 35.0 2.0 36.0 88-89 27.779324105244623 33.0 26.5 35.0 2.0 35.0 90-91 27.211051544120387 33.0 26.0 35.0 2.0 35.0 92-93 26.54770409513062 32.0 25.0 35.0 2.0 35.0 94-95 25.765882498870198 32.0 23.5 34.0 2.0 35.0 96-97 24.90211296804374 32.0 19.0 34.0 2.0 35.0 98-99 23.82165567802425 31.0 2.0 34.0 2.0 35.0 100-101 20.287673362784805 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.03135016326159956 1101 2 0.014960017482586352 1101 3 0.11202432387332806 1101 4 -0.04406254332180026 1101 5 0.022978480209452812 1101 6 -0.043769709599359885 1101 7 -0.04122878726447965 1101 8 0.05806925619960168 1101 9 -0.014954099732783277 1101 10-11 0.10316224287959841 1101 12-13 0.08072274455320638 1101 14-15 0.14629469674888185 1101 16-17 0.16079171767382405 1101 18-19 0.1465099481826826 1101 20-21 0.18128361694657968 1101 22-23 0.13825603658412433 1101 24-25 0.1342592347111875 1101 26-27 0.18941409176238722 1101 28-29 0.1972804925248255 1101 30-31 0.2306906509972606 1101 32-33 0.3321054940711221 1101 34-35 0.16036688816453903 1101 36-37 0.20956497213860814 1101 38-39 0.24257061418127535 1101 40-41 0.28725485492877567 1101 42-43 0.3690042312910151 1101 44-45 0.3332680159318713 1101 46-47 0.37325411912331674 1101 48-49 0.4792627228935302 1101 50-51 0.4569082555477735 1101 52-53 0.29613648612448884 1101 54-55 0.39929141513946576 1101 56-57 0.5086639896878822 1101 58-59 0.5425881576772511 1101 60-61 0.5010391417771842 1101 62-63 0.5592030078060191 1101 64-65 0.6289720658545193 1101 66-67 0.5475381301890607 1101 68-69 0.6333196562618113 1101 70-71 0.7850750195287155 1101 72-73 0.7719588117739669 1101 74-75 0.8567987117486133 1101 76-77 0.920936531028552 1101 78-79 0.8970547256674415 1101 80-81 0.9605989303184579 1101 82-83 1.1040660220992145 1101 84-85 1.3369980638629677 1101 86-87 1.3885185680680507 1101 88-89 1.599896117877428 1101 90-91 1.6830052695311473 1101 92-93 1.9446286327832425 1101 94-95 1.9824915044386415 1101 96-97 2.2785399024272586 1101 98-99 2.3426347753077295 1101 100-101 2.6435131795855185 1102 1 0.06383210315063081 1102 2 0.09503537036559351 1102 3 0.20218755257905485 1102 4 0.04943590282632471 1102 5 -0.0419569872612513 1102 6 0.005128301840464644 1102 7 0.03623822970584456 1102 8 -0.008683092228274347 1102 9 0.042266134823726986 1102 10-11 0.1437982997334899 1102 12-13 0.1769950498288111 1102 14-15 0.19410755176819094 1102 16-17 0.16833636912154049 1102 18-19 0.19934188034442712 1102 20-21 0.17990411332680623 1102 22-23 0.3014367427332729 1102 24-25 0.1635224045690009 1102 26-27 0.2803895465422528 1102 28-29 0.10583289838000098 1102 30-31 0.2318883804234133 1102 32-33 0.08806691274067191 1102 34-35 0.13046422614658582 1102 36-37 0.19100011222204216 1102 38-39 0.27329508066486596 1102 40-41 0.25842002212873894 1102 42-43 0.3109068743674257 1102 44-45 0.3605105756741338 1102 46-47 0.3859859538652799 1102 48-49 0.45602522226393916 1102 50-51 0.5324124556186263 1102 52-53 0.4675956130831693 1102 54-55 0.4538864170702155 1102 56-57 0.5858570000880761 1102 58-59 0.6141261503133038 1102 60-61 0.6101397114439351 1102 62-63 0.4777412634711595 1102 64-65 0.5449253396626332 1102 66-67 0.564421820965002 1102 68-69 0.6122531142075118 1102 70-71 0.8120054743796281 1102 72-73 0.7867920810361149 1102 74-75 0.9029257698651314 1102 76-77 0.780323591461098 1102 78-79 1.0380955718031757 1102 80-81 1.16520004520018 1102 82-83 1.2299211356903257 1102 84-85 1.2783914378546015 1102 86-87 1.2986866010366604 1102 88-89 1.3788912003958096 1102 90-91 1.6310768272186742 1102 92-93 1.7665793572325086 1102 94-95 1.8222671849672132 1102 96-97 2.013631297501682 1102 98-99 2.35492417446061 1102 100-101 2.7407140507240833 1103 1 0.23462877588590914 1103 2 0.35938638654379673 1103 3 0.3841135002847409 1103 4 0.28436483169411275 1103 5 0.12482192377339629 1103 6 0.16566406206786155 1103 7 0.19120094059110215 1103 8 0.18918008904801553 1103 9 0.1906983976339518 1103 10-11 0.25712697764322456 1103 12-13 0.3034143889208636 1103 14-15 0.38897442960266915 1103 16-17 0.39557349600392655 1103 18-19 0.4855045827401696 1103 20-21 0.4358498726379594 1103 22-23 0.49318126868502077 1103 24-25 0.5170007980026838 1103 26-27 0.4064190931250522 1103 28-29 0.45551806198673717 1103 30-31 0.4956037190433591 1103 32-33 0.44457538645711026 1103 34-35 0.5754360580730236 1103 36-37 0.5665136884414963 1103 38-39 0.7301548076444604 1103 40-41 0.8892058914514536 1103 42-43 0.7028207245287064 1103 44-45 0.7522570945889058 1103 46-47 0.7962838526610696 1103 48-49 0.9145548969657824 1103 50-51 0.9268114271461769 1103 52-53 0.8212201140012212 1103 54-55 0.8564089129639356 1103 56-57 0.7771189442778663 1103 58-59 0.6961677675225602 1103 60-61 0.756346973387096 1103 62-63 0.9203775756716652 1103 64-65 0.7462478647519717 1103 66-67 0.6819946042336369 1103 68-69 0.7913613486648572 1103 70-71 0.9040344246264702 1103 72-73 0.7985734690390949 1103 74-75 0.928560321528682 1103 76-77 0.5885195354448598 1103 78-79 0.8776359065277859 1103 80-81 1.0992320630571264 1103 82-83 1.1115151903451341 1103 84-85 1.2688925101722432 1103 86-87 1.294251352405464 1103 88-89 1.3680180341159165 1103 90-91 1.5176522541398292 1103 92-93 1.8819882226567763 1103 94-95 1.9891246498043564 1103 96-97 2.087236728092993 1103 98-99 2.5117051405766198 1103 100-101 2.587859998253766 1104 1 0.0921048282214727 1104 2 0.09620978361915888 1104 3 0.15534646458525714 1104 4 0.10226880422386131 1104 5 0.04693239680636907 1104 6 0.028789987116702775 1104 7 0.07483246033534385 1104 8 0.05288999373098591 1104 9 0.0758305982141394 1104 10-11 0.11344927946076666 1104 12-13 0.06095532788243929 1104 14-15 0.1836621331134154 1104 16-17 0.23922182943592674 1104 18-19 0.18400165218543663 1104 20-21 0.16959103657141128 1104 22-23 0.10929635956181727 1104 24-25 0.15478328571803246 1104 26-27 0.03173529702061728 1104 28-29 0.02489429785198638 1104 30-31 0.13163900410641816 1104 32-33 0.17026954310858144 1104 34-35 0.0739740960814288 1104 36-37 0.18594486480760963 1104 38-39 0.07983534757696731 1104 40-41 0.2701364461847433 1104 42-43 0.2754620127887293 1104 44-45 0.3707169844710876 1104 46-47 0.3209221807647893 1104 48-49 0.18740304094778537 1104 50-51 0.3279186440072266 1104 52-53 0.3711635158128388 1104 54-55 0.3410716327555363 1104 56-57 0.40464567448922395 1104 58-59 0.35754921326289946 1104 60-61 0.4155582693435207 1104 62-63 0.44231422832692147 1104 64-65 0.3485552473817748 1104 66-67 0.4042854099881765 1104 68-69 0.5169973678466633 1104 70-71 0.7110565073924384 1104 72-73 0.5955996960238785 1104 74-75 0.5931714161944832 1104 76-77 0.5085960258835556 1104 78-79 0.7353305368042662 1104 80-81 0.9246684492983199 1104 82-83 0.9590736534319859 1104 84-85 1.0187848788893064 1104 86-87 1.1625149247220783 1104 88-89 1.250768256127639 1104 90-91 1.115381814260605 1104 92-93 1.2430640618610838 1104 94-95 1.484353943511067 1104 96-97 1.7246428023337117 1104 98-99 2.126322629180013 1104 100-101 2.6005242573266436 1105 1 0.11911877182733832 1105 2 0.10105755485549395 1105 3 0.20852711174563154 1105 4 0.06402720358644132 1105 5 0.03403044470154981 1105 6 0.053266105925075635 1105 7 0.05808154182797409 1105 8 0.05425230615628607 1105 9 0.025794389104319748 1105 10-11 0.07856193494303199 1105 12-13 0.12398157727378845 1105 14-15 0.09289280019321922 1105 16-17 0.11441044437108161 1105 18-19 0.16985380569790465 1105 20-21 0.11992426661878142 1105 22-23 0.16879793098406992 1105 24-25 0.126615696855211 1105 26-27 0.16815395931975985 1105 28-29 0.0682795397624858 1105 30-31 0.09759128598857814 1105 32-33 0.07860914737576508 1105 34-35 0.18917457414791272 1105 36-37 0.2542370165494532 1105 38-39 0.25093206750968733 1105 40-41 0.30701405231108225 1105 42-43 0.15151734455800892 1105 44-45 0.13114786402488932 1105 46-47 0.14073467246502247 1105 48-49 0.21582019822280785 1105 50-51 0.23796016496689987 1105 52-53 0.3359882690331446 1105 54-55 0.3402067750688502 1105 56-57 0.2604686128274949 1105 58-59 0.3524943684131827 1105 60-61 0.39564600339690514 1105 62-63 0.4964981068626173 1105 64-65 0.6153706163340118 1105 66-67 0.7011507988598566 1105 68-69 0.8066451036706681 1105 70-71 0.9238443432562811 1105 72-73 0.7328304516345234 1105 74-75 0.6711827481408328 1105 76-77 0.7322730654125706 1105 78-79 1.074942771765091 1105 80-81 1.1665171501083798 1105 82-83 1.0778550265451727 1105 84-85 1.3566480987556275 1105 86-87 1.5133032917709066 1105 88-89 1.5770559813279377 1105 90-91 1.396909856401276 1105 92-93 1.7287959019744044 1105 94-95 1.860808372265879 1105 96-97 2.1132433069594505 1105 98-99 2.3641915150195096 1105 100-101 2.6431491079308636 1106 1 -0.0292687697077767 1106 2 0.035734373783093076 1106 3 0.08897114103046988 1106 4 0.030820141583653538 1106 5 0.02244404477875861 1106 6 0.02254322266458786 1106 7 0.09652788742766205 1106 8 -0.04115746097134121 1106 9 -0.07691487201881841 1106 10-11 0.025671022342557137 1106 12-13 0.00630606127329969 1106 14-15 -0.03392199113162775 1106 16-17 0.025342623223679084 1106 18-19 -0.02314761442369928 1106 20-21 -0.02330413167707235 1106 22-23 9.162905367929852E-4 1106 24-25 -0.017944065446762636 1106 26-27 0.004171448875666783 1106 28-29 -0.07632744502981126 1106 30-31 0.04963684707449545 1106 32-33 0.0879679900455983 1106 34-35 -0.16454531720589216 1106 36-37 -0.07185323700430502 1106 38-39 -0.09408268520991925 1106 40-41 -0.1745805512268177 1106 42-43 -0.2017411796185229 1106 44-45 -0.03504789904918226 1106 46-47 -0.26467661129733244 1106 48-49 -0.011375647576805648 1106 50-51 0.0049156241625283315 1106 52-53 -0.04380067745722016 1106 54-55 -0.0021225001176290448 1106 56-57 0.11790408691372534 1106 58-59 -0.07186709389844737 1106 60-61 0.054719615331443094 1106 62-63 0.16549757604046533 1106 64-65 0.16660874177348717 1106 66-67 0.043695677460135585 1106 68-69 -0.0843891722741219 1106 70-71 0.08798489393256048 1106 72-73 0.03832618437590796 1106 74-75 0.2013970417337383 1106 76-77 0.2317189767032275 1106 78-79 0.38148670591556666 1106 80-81 0.4316429963216173 1106 82-83 0.4370158449764219 1106 84-85 0.5856589082249428 1106 86-87 0.8340119048773893 1106 88-89 0.8284723113476531 1106 90-91 0.8263503215945107 1106 92-93 0.7979234233796362 1106 94-95 1.0566300435973446 1106 96-97 1.139872310530432 1106 98-99 1.3719365204656064 1106 100-101 1.6227951805363041 1107 1 0.09117032153675808 1107 2 0.04110045886032054 1107 3 0.142670208027468 1107 4 0.04063248674205511 1107 5 -0.005577672579313742 1107 6 -0.01139278492594542 1107 7 -0.04711372345209952 1107 8 0.0027925153082435372 1107 9 0.034642241470187685 1107 10-11 -0.02544647278158152 1107 12-13 -0.042268737103178466 1107 14-15 0.05122771104538515 1107 16-17 0.11635653920069444 1107 18-19 0.04865540070464647 1107 20-21 -0.028915986613561984 1107 22-23 0.004380130081358402 1107 24-25 0.17139294894361967 1107 26-27 0.06758797936957706 1107 28-29 2.723652068752358E-4 1107 30-31 -0.03296422825100365 1107 32-33 0.04172833478229876 1107 34-35 -0.008959818286484733 1107 36-37 0.03435821732072242 1107 38-39 0.026189045326169946 1107 40-41 0.1483557726359166 1107 42-43 0.22211694594154352 1107 44-45 0.2578985718801263 1107 46-47 0.2961491227177433 1107 48-49 0.3167020763175614 1107 50-51 0.4306647939479902 1107 52-53 0.30972443982705755 1107 54-55 0.32005081705067795 1107 56-57 0.3480547115513275 1107 58-59 0.45618163837713155 1107 60-61 0.5821305319937125 1107 62-63 0.5502947664890456 1107 64-65 0.5080575320323035 1107 66-67 0.6117996255915017 1107 68-69 0.6844547210367224 1107 70-71 0.5755488072739787 1107 72-73 0.6209648170257402 1107 74-75 0.7855933149929264 1107 76-77 0.6494256078771237 1107 78-79 0.708476585205009 1107 80-81 0.7011464489598573 1107 82-83 0.8686687625875145 1107 84-85 1.125976366450658 1107 86-87 1.1652273126049693 1107 88-89 1.2821964165608755 1107 90-91 1.2783006668583319 1107 92-93 1.6098172349868278 1107 94-95 1.6518345662898781 1107 96-97 1.5396074325587925 1107 98-99 1.836826352314219 1107 100-101 2.1841783443125955 1108 1 0.06141736471325743 1108 2 0.0356804650771636 1108 3 0.14465883425745574 1108 4 0.04735062923011668 1108 5 0.00976315833777619 1108 6 0.0448263537861493 1108 7 -0.01764230820248258 1108 8 0.029210287333455653 1108 9 0.015089752438846915 1108 10-11 0.05020922607849343 1108 12-13 0.03924200733860772 1108 14-15 0.10929980097562009 1108 16-17 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4505.0 13 6454.0 14 9118.0 15 12405.0 16 15853.0 17 22124.0 18 22331.0 19 17478.0 20 15727.0 21 15902.0 22 16695.0 23 18141.0 24 20391.0 25 23790.0 26 27700.0 27 33234.0 28 40207.0 29 47982.0 30 58868.0 31 72864.0 32 90481.0 33 118247.0 34 163380.0 35 241072.0 36 364634.0 37 493204.0 38 307481.0 39 18908.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.82148609931674 18.945498010594548 15.334989508672802 22.89802638141591 2 15.703987091401208 19.85577590394892 40.13411756541051 24.306119439239364 3 19.626705320748204 23.541385283863743 29.84366610021435 26.988243295173703 4 13.048973116353546 17.039580797965403 36.648777775561065 33.26266831011999 5 13.778154042055629 37.07598707208371 34.35365623053743 14.792202655323225 6 32.43657373935739 36.26945117532178 16.930540671004017 14.363434414316812 7 28.615127461601915 32.736020165553924 20.12439530661652 18.524457066227644 8 26.422392051735887 35.0510970368793 19.22157633805798 19.30493457332684 9 25.881310518130174 16.93054732720537 20.15987238660129 37.02826976806317 10-11 25.003881660951876 26.624681118300014 26.902209033730983 21.469228187017123 12-13 26.027859483006132 23.741929831713733 27.453556685694757 22.776653999585378 14-15 22.594411102157512 25.375653702063655 25.921905318699544 26.108029877079293 16-17 22.030613537163358 28.492283511557385 26.919905868556004 22.557197082723253 18-19 21.980306317262244 26.743884974459103 29.44278865462065 21.833020053658 20-21 22.39414775639997 25.900003209417992 29.92845166399649 21.777397370185547 22-23 22.186364437732614 25.820228749148583 29.882442029332783 22.110964783786017 24-25 22.17533062479602 26.001394387103982 29.874516003465367 21.948758984634633 26-27 21.69395808832365 26.356303474651234 29.686454122257643 22.263284314767475 28-29 21.864079404729093 26.785477683304443 29.464027620167617 21.886415291798848 30-31 21.906433532252667 26.282030013768004 29.47159192066126 22.339944533318068 32-33 21.615374873353595 26.327748747415026 29.468687977960055 22.58818840127132 34-35 22.42735759764095 26.475729764103594 29.157185032624337 21.939727605631116 36-37 22.46319608211391 25.981217325828474 29.280850511964935 22.27473608009268 38-39 21.955532111118533 26.408762917455817 29.14796630594817 22.487738665477476 40-41 22.459012090909898 26.21916078331171 29.23762608389911 22.08420104187928 42-43 22.15510027732266 26.210591704091996 29.060816212682578 22.57349180590277 44-45 22.187951234471246 26.490936164730915 29.16877215643937 22.152340444358465 46-47 22.605202546169544 26.811330987945098 28.370993201728183 22.212473264157175 48-49 22.291805633502275 26.851905663601517 28.347456267191045 22.50883243570516 50-51 22.51005545353847 27.0318434983697 28.34707600335514 22.111025044736685 52-53 22.780232066943253 26.719987821559833 28.26560449387912 22.234175617617797 54-55 22.361554815558218 26.944018208676358 28.328903758228467 22.365523217536953 56-57 22.393714051265682 27.09457894604448 28.1462054704096 22.365501532280238 58-59 22.396619875665415 26.80072203230756 28.093813890187597 22.708844201839433 60-61 22.102546109361352 26.995238785035784 28.36030400995093 22.54191109565193 62-63 22.17473632896361 26.963925274340184 28.525090275723702 22.336248120972506 64-65 21.912539890029727 27.088138424800302 28.290173889736543 22.70914779543343 66-67 21.85862489211566 27.08595183262422 28.209098282090984 22.84632499316913 68-69 22.410662570170885 26.714476947561284 28.387865692592367 22.48699478967546 70-71 22.28719607028452 26.92680011484512 27.927921676322388 22.85808213854797 72-73 22.216740671219416 26.987800741982742 27.939566659178023 22.85589192761982 74-75 22.268806972590703 27.38813226618739 27.689795872341495 22.653264888880408 76-77 22.297258029400005 27.005105576840837 27.999678190790355 22.6979582029688 78-79 22.331824328602767 27.199231994948203 27.93451399436357 22.534429682085463 80-81 22.49789761162609 27.236847983116704 27.688986173896033 22.576268231361173 82-83 22.50391584495184 27.28984495574331 27.77917648745184 22.42706271185301 84-85 22.35959853914083 27.464495054387534 27.74326924599117 22.432637160480468 86-87 22.574313721918482 27.367851297090944 27.53573971959901 22.522095261391563 88-89 22.45075114492759 27.599612874713188 27.20327135179631 22.746364628562915 90-91 22.320597145688716 27.542997915975427 27.43374384930832 22.702661089027533 92-93 22.38520726252646 27.248764908168845 27.60902442166128 22.75700340764341 94-95 22.254186346071275 27.01623361133881 27.704069462334814 23.02551058025511 96-97 22.42549084196102 26.759932367801365 27.713501263061886 23.101075527175727 98-99 22.155115845061108 27.049835863935996 27.37162408612709 23.42342420487581 100-101 22.450822893020003 26.50814565635835 27.70353731665659 23.33749413396506 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 901.0 1 609.5 2 440.0 3 573.5 4 461.5 5 426.0 6 857.5 7 2034.0 8 3338.0 9 3876.5 10 3214.0 11 2355.0 12 2236.0 13 2349.0 14 2528.0 15 2639.5 16 2805.5 17 3080.0 18 3355.5 19 3643.5 20 4036.5 21 4800.0 22 5874.5 23 7377.0 24 9254.0 25 11571.0 26 14668.5 27 18206.0 28 21426.0 29 24713.5 30 28412.0 31 33005.0 32 39395.0 33 42712.5 34 43136.5 35 45538.5 36 49744.5 37 54103.0 38 57655.5 39 60640.0 40 64345.0 41 69166.5 42 71461.0 43 73558.0 44 77608.0 45 82136.0 46 84064.5 47 84866.5 48 85860.0 49 84231.0 50 82311.5 51 80643.5 52 79417.5 53 79832.0 54 80808.5 55 76671.5 56 70447.5 57 64410.5 58 59577.0 59 53355.0 60 42502.0 61 34671.5 62 28946.5 63 21269.5 64 14642.0 65 10779.5 66 7918.0 67 6023.5 68 4567.5 69 3428.0 70 2624.0 71 1801.5 72 1247.0 73 969.5 74 698.5 75 479.0 76 330.5 77 205.0 78 126.0 79 62.5 80 33.5 81 21.5 82 16.5 83 13.5 84 9.5 85 4.5 86 2.5 87 2.5 88 2.0 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.011883520679494509 3 0.005898389826318442 4 0.0 5 2.6022308057287245E-4 6 2.1685256714406038E-4 7 0.0 8 1.3011154028643622E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 8.674102685762415E-5 18-19 0.0 20-21 0.0 22-23 2.1685256714406036E-5 24-25 0.0017998763072957012 26-27 0.023116483657556836 28-29 3.2527885071609053E-4 30-31 2.3853782385846643E-4 32-33 0.00355638210116259 34-35 0.016480795102948587 36-37 0.008023544984330232 38-39 0.021685256714406034 40-41 0.023962208669418672 42-43 0.019820324636967118 44-45 0.010105329628913213 46-47 0.002363692981870258 48-49 3.469641074304966E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.734820537152483E-4 68-69 1.5179679700084227E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.3011154028643622E-4 82-83 7.589839850042113E-4 84-85 0.00366480838473462 86-87 6.939282148609932E-4 88-89 2.1685256714406036E-5 90-91 0.0034262705608761536 92-93 6.505577014321811E-4 94-95 1.734820537152483E-4 96-97 3.2527885071609053E-4 98-99 2.1685256714406038E-4 100-101 0.002949194913159221 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2305714.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.60347319106138 #Duplication Level Percentage of deduplicated Percentage of total 1 75.87720994001366 42.190364087120855 2 13.81518732690323 15.363447963219096 3 4.572750979678177 7.627825095238055 4 1.976684188082337 4.396420250369246 5 1.051891598034443 2.924441313560543 6 0.6173469001519951 2.059597908731179 7 0.40633374655344556 1.5815497308175632 8 0.2930929712591523 1.3037589735917439 9 0.2030196102802113 1.0159735911727914 >10 1.0769434830486766 10.777912858194206 >50 0.06878620470669053 2.610703244967911 >100 0.03466896687068002 3.5738496821319305 >500 0.0034320476199658453 1.2985370474423936 >1k 0.0024180335504298575 2.215363187919067 >5k 1.5600216454386177E-4 0.6199061462622062 >10k+ 7.800108227193089E-5 0.44034891926118935 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10153 0.44034082284272896 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8815 0.3823110758749784 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5478 0.23758367256303253 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2859 0.12399629789297373 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2826 0.12256507094982293 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2671 0.11584264136835704 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2637 0.11436804391177742 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2457 0.10656135149459127 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.337051342881207E-5 2 0.0 0.0 0.0 0.0 4.337051342881207E-5 3 0.0 0.0 0.0 0.0 4.337051342881207E-5 4 0.0 0.0 0.0 0.0 4.337051342881207E-5 5 0.0 0.0 0.0 0.0 4.337051342881207E-5 6 0.0 0.0 0.0 0.0 4.337051342881207E-5 7 0.0 0.0 0.0 0.0 4.337051342881207E-5 8 0.0 0.0 0.0 0.0 4.337051342881207E-5 9 0.0 0.0 0.0 0.0 4.337051342881207E-5 10-11 0.0 0.0 0.0 0.0 4.337051342881207E-5 12-13 0.0 0.0 0.0 0.0 3.2527885071609053E-4 14-15 0.0 0.0 0.0 0.0 4.1201987757371467E-4 16-17 0.0 0.0 0.0 0.0 4.770756477169328E-4 18-19 2.1685256714406036E-5 0.0 0.0 2.1685256714406036E-5 5.204461611457449E-4 20-21 4.337051342881207E-5 0.0 0.0 1.0842628357203018E-4 7.156134715753992E-4 22-23 4.337051342881207E-5 0.0 0.0 2.6022308057287245E-4 7.372987282898053E-4 24-25 4.337051342881207E-5 0.0 0.0 5.63816674574557E-4 7.372987282898053E-4 26-27 4.337051342881207E-5 0.0 0.0 0.0013011154028643624 8.023544984330234E-4 28-29 4.337051342881207E-5 0.0 0.0 0.0030142506833024394 8.674102685762414E-4 30-31 4.337051342881207E-5 0.0 0.0 0.009042752049907317 8.890955252906475E-4 32-33 4.337051342881207E-5 0.0 0.0 0.02326828045455768 9.107807820050535E-4 34-35 4.337051342881207E-5 0.0 0.0 0.04415118267053069 9.541512954338656E-4 36-37 4.337051342881207E-5 0.0 0.0 0.07776333057786006 9.541512954338656E-4 38-39 4.337051342881207E-5 0.0 0.0 0.1286152575731422 9.975218088626777E-4 40-41 4.337051342881207E-5 0.0 0.0 0.17964066662213962 9.975218088626777E-4 42-43 4.337051342881207E-5 0.0 0.0 0.23337673276043774 9.975218088626777E-4 44-45 4.337051342881207E-5 0.0 0.0 0.2988011522678008 0.0010625775790058958 46-47 4.337051342881207E-5 0.0 0.0 0.3788848053141023 0.00114931860586352 48-49 4.337051342881207E-5 0.0 0.0 0.4641512347151468 0.001214374376006738 50-51 4.337051342881207E-5 0.0 0.0 0.5547088667545064 0.00125774488943555 52-53 6.505577014321811E-5 0.0 0.0 0.6400403519256942 0.0015179679700084226 54-55 8.674102685762415E-5 0.0 0.0 0.7323328045022062 0.0015613384834372347 56-57 8.674102685762415E-5 0.0 0.0 0.8405856060205212 0.0015830237401516407 58-59 8.674102685762415E-5 0.0 0.0 0.9548452236487266 0.0016047089968660468 60-61 8.674102685762415E-5 0.0 0.0 1.0740707650645311 0.0016047089968660468 62-63 8.674102685762415E-5 0.0 0.0 1.190282055797033 0.001778191050581295 64-65 8.674102685762415E-5 0.0 0.0 1.3084667048905458 0.001821561564010107 66-67 8.674102685762415E-5 0.0 0.0 1.449312447250613 0.001821561564010107 68-69 8.674102685762415E-5 0.0 0.0 1.6047523673794755 0.001821561564010107 70-71 8.674102685762415E-5 0.0 0.0 1.7667846055495176 0.001821561564010107 72-73 8.674102685762415E-5 0.0 0.0 1.929554142447849 0.0019516731042965433 74-75 8.674102685762415E-5 0.0 0.0 2.0964221928652034 0.0020817846445829796 76-77 8.674102685762415E-5 0.0 0.0 2.278079588361783 0.0020817846445829796 78-79 8.674102685762415E-5 0.0 0.0 2.4781477668088927 0.0021685256714406038 80-81 8.674102685762415E-5 0.0 0.0 2.6933088839292294 0.00219021092815501 82-83 8.674102685762415E-5 0.0 0.0 2.9128504229058763 0.00240706349529907 84-85 8.674102685762415E-5 0.0 0.0 3.133562965745101 0.002428748752013476 86-87 8.674102685762415E-5 0.0 0.0 3.3626893881895152 0.0024938045221566943 88-89 8.674102685762415E-5 0.0 0.0 3.61298062118719 0.002558860292299912 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4875 0.0 50.67174 1 GTATCAA 8440 0.0 39.79368 1 ATCAACG 10745 0.0 30.98992 3 TCAACGC 10880 0.0 30.819683 4 TATCAAC 10985 0.0 30.531052 2 CAACGCA 11060 0.0 30.361042 5 AACGCAG 11470 0.0 29.275772 6 ACGCAGA 12765 0.0 26.045313 7 CGCAGAG 12930 0.0 25.639484 8 TGGTATC 2320 0.0 24.161905 2 GTGGTAT 2470 0.0 24.04087 1 TAAGGTG 1550 0.0 23.900955 5 GCAGAGT 14255 0.0 22.889795 9 GAGTACT 9475 0.0 22.131144 12-13 GTACATG 7070 0.0 21.501448 1 AGAGTAC 12370 0.0 21.444004 10-11 GTACTTT 9720 0.0 21.377857 14-15 GTAAGGT 1535 0.0 21.349764 4 TACATGG 6845 0.0 21.236607 2 GTGTAAG 1025 0.0 20.85575 1 >>END_MODULE