FastQCFastQC Report
Thu 26 May 2016
SRR938001_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938001_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2309201
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108140.4683005074049422No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85420.36991149752663366No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54960.23800440065633088No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28330.12268312719421134No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC26480.11467169813281737No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26150.1132426324083525No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA25310.10960501056426011No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA23470.10163688652481961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47600.054.3623281
GTATCAA82900.040.4861561
ATCAACG105300.031.3203493
TATCAAC105850.031.292292
TCAACGC106300.031.0257054
CAACGCA109300.030.0002165
AACGCAG113100.029.0342716
TAAGGTG14550.028.0886545
ACGCAGA126850.025.6739887
CGCAGAG127400.025.56268
GTAAGGT15250.024.3063774
GTACATG73750.023.822731
TGGTATC21600.023.3210222
TACATGG71000.023.225582
GCAGAGT140050.022.8797459
GTGGTAT23050.022.7838691
GAGTACT93350.022.5867512-13
GGTAAGG16100.022.4327853
ACATGGG72000.021.714953
GTACTTT96650.021.57734914-15
Produced by FastQC (version 0.11.5)